Leukocyte migration inhibition by dental plaque antigens as indicated by the capillary tube and the agarose plate technique
Identifieur interne : 006659 ( Istex/Curation ); précédent : 006658; suivant : 006660Leukocyte migration inhibition by dental plaque antigens as indicated by the capillary tube and the agarose plate technique
Auteurs : E. Budtz-Jörgensen [Danemark] ; J. Kelstrup [Danemark] ; A. Budtz-Jörgensen [Danemark]Source :
- Journal of Periodontal Research [ 0022-3484 ] ; 1976-04.
Descripteurs français
- Wicri :
- topic : Migration.
English descriptors
- KwdEn :
- Acta, Acta aliergol, Actinomyces viscosus, Agarose, Agarose plate migration technique, Agarose plate technique, Antigen, Antigen concentration, Bacterial antigens, Bacteroides melaninogenicus, Candida, Candida albicans, Capillary tube technique, Cell migration, Cellular immunity, Clausen, Control cultures, Culture chambers, Dental plaque antigens, Edentulous, Edentulous persons, Fusobacterium nucleatum, Horse serum, Hypersensitivity, Inflammation, Inhibition, Leucocyte migration test, Leukocyte, Leukocyte migration inhibition, Leukocyte migration test, Melaninogenicus, Migration, Migration area, Migration areas, Migration index, Migration indices, Migration inhibition, Mild gingivitis, Mygind stenbjerg, Natural teeth, Nucleatum, Parvula, Parvula antigen, Periodontal, Periodontal disease, Plaque, Plaque antigens, Polymorphonuclear leukocytes, Protein synthesis, Puromycin, Rosenberg david, Skin test, Soluble, Soluble antigens, Tuberculin, Veillonelia, Veillonelia antigen, Veillonelia parvula, Viscosus.
- Teeft :
- Acta, Acta aliergol, Actinomyces viscosus, Agarose, Agarose plate migration technique, Agarose plate technique, Antigen, Antigen concentration, Bacterial antigens, Bacteroides melaninogenicus, Candida, Candida albicans, Capillary tube technique, Cell migration, Cellular immunity, Clausen, Control cultures, Culture chambers, Dental plaque antigens, Edentulous, Edentulous persons, Fusobacterium nucleatum, Horse serum, Hypersensitivity, Inflammation, Inhibition, Leucocyte migration test, Leukocyte, Leukocyte migration inhibition, Leukocyte migration test, Melaninogenicus, Migration, Migration area, Migration areas, Migration index, Migration indices, Migration inhibition, Mild gingivitis, Mygind stenbjerg, Natural teeth, Nucleatum, Parvula, Parvula antigen, Periodontal, Periodontal disease, Plaque, Plaque antigens, Polymorphonuclear leukocytes, Protein synthesis, Puromycin, Rosenberg david, Skin test, Soluble, Soluble antigens, Tuberculin, Veillonelia, Veillonelia antigen, Veillonelia parvula, Viscosus.
Abstract
The response of peripheral blood leukocytes to various concentrations of whole cells of Candida albicans (particulate antigen), and of PPD and ultrasonicates of Veillonella parvula, Bacteroides melaninogenicus, Actinomyces viscosus, and Fusobacterium nucleatum (soluble antigens) was studied in vitro by means of two different leukocyte migration tests: The capillary tube technique and the agarose plate technique. Using the capillary tube technique migration inhibition to Candida cells was significant in persons with a delayed hypersensitivity skin reaction to this antigen; however, migration inhibition to PPD or the soluble antigens of dental plaque bacteria was not detected in persons with mild periodontal inflammation. With the agarose plate technique the migration of leukocytes from tuberculin positive persons was inhibited by PPD, while no inhibition of cells from tuberculin negative persons was found. Migration inhibition to soluble V. parvula antigen was significant in persons with mild gingivitis as compared with edentulous persons. Migration inhibition to antigens from the other dental plaque bacteria was not significant. Blocking of protein synthesis by means of puromycin prevented migration inhibition by PPD and V. parvula. The results suggest that the leukocyte migration test in agarose is suitable faor detection of cell‐mediated immunity to bacteria involved in periodontal disease.
Url:
DOI: 10.1111/j.1600-0765.1976.tb00055.x
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :006659
Links to Exploration step
ISTEX:CE5D011B58174F00295ACEE0532CBDC3A91AD8BCLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Leukocyte migration inhibition by dental plaque antigens as indicated by the capillary tube and the agarose plate technique</title><author><name sortKey="Budtz Rgensen, E" sort="Budtz Rgensen, E" uniqKey="Budtz Rgensen E" first="E." last="Budtz-Jörgensen">E. Budtz-Jörgensen</name><affiliation wicri:level="1"><mods:affiliation>Departments of Prosthetic Dentistry and Microbiology, Royal Dental College, Århus, Denmark</mods:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Departments of Prosthetic Dentistry and Microbiology, Royal Dental College, Århus</wicri:regionArea></affiliation><affiliation><mods:affiliation>Correspondence address: Address:</mods:affiliation><wicri:noCountry code="no comma">Correspondence address: Address:</wicri:noCountry></affiliation></author><author><name sortKey="Kelstrup, J" sort="Kelstrup, J" uniqKey="Kelstrup J" first="J." last="Kelstrup">J. Kelstrup</name><affiliation wicri:level="1"><mods:affiliation>Departments of Prosthetic Dentistry and Microbiology, Royal Dental College, Århus, Denmark</mods:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Departments of Prosthetic Dentistry and Microbiology, Royal Dental College, Århus</wicri:regionArea></affiliation><affiliation><mods:affiliation>Correspondence address: Address:</mods:affiliation><wicri:noCountry code="no comma">Correspondence address: Address:</wicri:noCountry></affiliation></author><author><name sortKey="Budtz Rgensen, A" sort="Budtz Rgensen, A" uniqKey="Budtz Rgensen A" first="A." last="Budtz-Jörgensen">A. Budtz-Jörgensen</name><affiliation wicri:level="1"><mods:affiliation>Departments of Prosthetic Dentistry and Microbiology, Royal Dental College, Århus, Denmark</mods:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Departments of Prosthetic Dentistry and Microbiology, Royal Dental College, Århus</wicri:regionArea></affiliation><affiliation><mods:affiliation>Correspondence address: Address:</mods:affiliation><wicri:noCountry code="no comma">Correspondence address: Address:</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:CE5D011B58174F00295ACEE0532CBDC3A91AD8BC</idno><date when="1976" year="1976">1976</date><idno type="doi">10.1111/j.1600-0765.1976.tb00055.x</idno><idno type="url">https://api.istex.fr/document/CE5D011B58174F00295ACEE0532CBDC3A91AD8BC/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">006659</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">006659</idno><idno type="wicri:Area/Istex/Curation">006659</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Leukocyte migration inhibition by dental plaque antigens as indicated by the capillary tube and the agarose plate technique</title><author><name sortKey="Budtz Rgensen, E" sort="Budtz Rgensen, E" uniqKey="Budtz Rgensen E" first="E." last="Budtz-Jörgensen">E. Budtz-Jörgensen</name><affiliation wicri:level="1"><mods:affiliation>Departments of Prosthetic Dentistry and Microbiology, Royal Dental College, Århus, Denmark</mods:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Departments of Prosthetic Dentistry and Microbiology, Royal Dental College, Århus</wicri:regionArea></affiliation><affiliation><mods:affiliation>Correspondence address: Address:</mods:affiliation></affiliation></author><author><name sortKey="Kelstrup, J" sort="Kelstrup, J" uniqKey="Kelstrup J" first="J." last="Kelstrup">J. Kelstrup</name><affiliation wicri:level="1"><mods:affiliation>Departments of Prosthetic Dentistry and Microbiology, Royal Dental College, Århus, Denmark</mods:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Departments of Prosthetic Dentistry and Microbiology, Royal Dental College, Århus</wicri:regionArea></affiliation><affiliation><mods:affiliation>Correspondence address: Address:</mods:affiliation></affiliation></author><author><name sortKey="Budtz Rgensen, A" sort="Budtz Rgensen, A" uniqKey="Budtz Rgensen A" first="A." last="Budtz-Jörgensen">A. Budtz-Jörgensen</name><affiliation wicri:level="1"><mods:affiliation>Departments of Prosthetic Dentistry and Microbiology, Royal Dental College, Århus, Denmark</mods:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Departments of Prosthetic Dentistry and Microbiology, Royal Dental College, Århus</wicri:regionArea></affiliation><affiliation><mods:affiliation>Correspondence address: Address:</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Periodontal Research</title><title level="j" type="alt">JOURNAL OF PERIODONTAL RESEARCH</title><idno type="ISSN">0022-3484</idno><idno type="eISSN">1600-0765</idno><imprint><biblScope unit="vol">11</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="86">86</biblScope><biblScope unit="page" to="95">95</biblScope><biblScope unit="page-count">10</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1976-04">1976-04</date></imprint><idno type="ISSN">0022-3484</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-3484</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acta</term><term>Acta aliergol</term><term>Actinomyces viscosus</term><term>Agarose</term><term>Agarose plate migration technique</term><term>Agarose plate technique</term><term>Antigen</term><term>Antigen concentration</term><term>Bacterial antigens</term><term>Bacteroides melaninogenicus</term><term>Candida</term><term>Candida albicans</term><term>Capillary tube technique</term><term>Cell migration</term><term>Cellular immunity</term><term>Clausen</term><term>Control cultures</term><term>Culture chambers</term><term>Dental plaque antigens</term><term>Edentulous</term><term>Edentulous persons</term><term>Fusobacterium nucleatum</term><term>Horse serum</term><term>Hypersensitivity</term><term>Inflammation</term><term>Inhibition</term><term>Leucocyte migration test</term><term>Leukocyte</term><term>Leukocyte migration inhibition</term><term>Leukocyte migration test</term><term>Melaninogenicus</term><term>Migration</term><term>Migration area</term><term>Migration areas</term><term>Migration index</term><term>Migration indices</term><term>Migration inhibition</term><term>Mild gingivitis</term><term>Mygind stenbjerg</term><term>Natural teeth</term><term>Nucleatum</term><term>Parvula</term><term>Parvula antigen</term><term>Periodontal</term><term>Periodontal disease</term><term>Plaque</term><term>Plaque antigens</term><term>Polymorphonuclear leukocytes</term><term>Protein synthesis</term><term>Puromycin</term><term>Rosenberg david</term><term>Skin test</term><term>Soluble</term><term>Soluble antigens</term><term>Tuberculin</term><term>Veillonelia</term><term>Veillonelia antigen</term><term>Veillonelia parvula</term><term>Viscosus</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acta</term><term>Acta aliergol</term><term>Actinomyces viscosus</term><term>Agarose</term><term>Agarose plate migration technique</term><term>Agarose plate technique</term><term>Antigen</term><term>Antigen concentration</term><term>Bacterial antigens</term><term>Bacteroides melaninogenicus</term><term>Candida</term><term>Candida albicans</term><term>Capillary tube technique</term><term>Cell migration</term><term>Cellular immunity</term><term>Clausen</term><term>Control cultures</term><term>Culture chambers</term><term>Dental plaque antigens</term><term>Edentulous</term><term>Edentulous persons</term><term>Fusobacterium nucleatum</term><term>Horse serum</term><term>Hypersensitivity</term><term>Inflammation</term><term>Inhibition</term><term>Leucocyte migration test</term><term>Leukocyte</term><term>Leukocyte migration inhibition</term><term>Leukocyte migration test</term><term>Melaninogenicus</term><term>Migration</term><term>Migration area</term><term>Migration areas</term><term>Migration index</term><term>Migration indices</term><term>Migration inhibition</term><term>Mild gingivitis</term><term>Mygind stenbjerg</term><term>Natural teeth</term><term>Nucleatum</term><term>Parvula</term><term>Parvula antigen</term><term>Periodontal</term><term>Periodontal disease</term><term>Plaque</term><term>Plaque antigens</term><term>Polymorphonuclear leukocytes</term><term>Protein synthesis</term><term>Puromycin</term><term>Rosenberg david</term><term>Skin test</term><term>Soluble</term><term>Soluble antigens</term><term>Tuberculin</term><term>Veillonelia</term><term>Veillonelia antigen</term><term>Veillonelia parvula</term><term>Viscosus</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Migration</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The response of peripheral blood leukocytes to various concentrations of whole cells of Candida albicans (particulate antigen), and of PPD and ultrasonicates of Veillonella parvula, Bacteroides melaninogenicus, Actinomyces viscosus, and Fusobacterium nucleatum (soluble antigens) was studied in vitro by means of two different leukocyte migration tests: The capillary tube technique and the agarose plate technique. Using the capillary tube technique migration inhibition to Candida cells was significant in persons with a delayed hypersensitivity skin reaction to this antigen; however, migration inhibition to PPD or the soluble antigens of dental plaque bacteria was not detected in persons with mild periodontal inflammation. With the agarose plate technique the migration of leukocytes from tuberculin positive persons was inhibited by PPD, while no inhibition of cells from tuberculin negative persons was found. Migration inhibition to soluble V. parvula antigen was significant in persons with mild gingivitis as compared with edentulous persons. Migration inhibition to antigens from the other dental plaque bacteria was not significant. Blocking of protein synthesis by means of puromycin prevented migration inhibition by PPD and V. parvula. The results suggest that the leukocyte migration test in agarose is suitable faor detection of cell‐mediated immunity to bacteria involved in periodontal disease.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV2/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006659 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 006659 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Santé
|area= EdenteV2
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:CE5D011B58174F00295ACEE0532CBDC3A91AD8BC
|texte= Leukocyte migration inhibition by dental plaque antigens as indicated by the capillary tube and the agarose plate technique
}}
| This area was generated with Dilib version V0.6.32. |  |