Alfacalcidol enhances collagen quality in ovariectomized rat bones
Identifieur interne : 004B10 ( Istex/Curation ); précédent : 004B09; suivant : 004B11Alfacalcidol enhances collagen quality in ovariectomized rat bones
Auteurs : Hideaki Nagaoka ; Masahiko Terajima ; Shizuka Yamada [Japon] ; Yoshiaki Azuma [Japon] ; Takayuki Chida [Japon] ; Mitsuo Yamauchi [États-Unis]Source :
- Journal of Orthopaedic Research [ 0736-0266 ] ; 2014-08.
Abstract
The aim of this study was to investigate the effects of alfacalcidol (1α(OH)D3: ALF) on bone collagen employing an ovariectomized rat model. Thirty‐five 16‐week‐old female Sprague‐Dawley rats were divided into five groups: SHAM (sham‐operated + vehicle), OVX (ovariectomy + vehicle), and three ALF‐treated groups, that is, ovariectomy + 0.022 µg/kg/day ALF, ovariectomy + 0.067 µg/kg/day ALF, and ovariectomy + 0.2 µg/kg/day ALF. After 12 weeks of treatment, tibiae were subjected to histological, biochemical and immunohistochemical analyses. Collagen matrices in OVX bone appeared as immature and poorly organized; however, with the ALF treatment, it was improved in a dose‐dependent manner. Contents of collagen and pyridinoline cross‐link were decreased in OVX compared with SHAM, but they increased to the level comparable to SHAM in ALF‐treated groups. The total aldehyde, that is, a sum of free and those involved cross‐links, in the highest dose of ALF was significantly higher than the rest of the groups (p < 0.05). In addition, the expression of lysyl oxidase was increased in the all ALF‐treated groups compared with OVX (p < 0.05). In conclusion, ALF increases not only the amount of collagen but also enhances the maturation of collagen in ovariectomy‐induced osteoporotic bones, which likely contributes to the improvement of bone quality. © 2014 The Authors. Journal of Orthopaedic Research. Published by Wiley Periodicals, Inc. J Orthop Res 32:1030–1036, 2014.
Url:
DOI: 10.1002/jor.22642
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :004B10
Links to Exploration step
ISTEX:966551EB5C792C71E58B588564A40EF5BD182BB6Curation
No country items
Hideaki Nagaoka<affiliation><mods:affiliation>NC Oral Health Institute, University of North Carolina at Chapel Hill, North Carolina, Chapel Hill</mods:affiliation>
<wicri:noCountry code="subField">Hill</wicri:noCountry>
</affiliation>
<affiliation><mods:affiliation>NC Oral Health Institute, University of North Carolina at Chapel Hill, North Carolina, Chapel Hill</mods:affiliation>
<wicri:noCountry code="subField">Hill</wicri:noCountry>
</affiliation>
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Alfacalcidol enhances collagen quality in ovariectomized rat bones</title>
<author><name sortKey="Nagaoka, Hideaki" sort="Nagaoka, Hideaki" uniqKey="Nagaoka H" first="Hideaki" last="Nagaoka">Hideaki Nagaoka</name>
<affiliation><mods:affiliation>NC Oral Health Institute, University of North Carolina at Chapel Hill, North Carolina, Chapel Hill</mods:affiliation>
<wicri:noCountry code="subField">Hill</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Terajima, Masahiko" sort="Terajima, Masahiko" uniqKey="Terajima M" first="Masahiko" last="Terajima">Masahiko Terajima</name>
<affiliation><mods:affiliation>NC Oral Health Institute, University of North Carolina at Chapel Hill, North Carolina, Chapel Hill</mods:affiliation>
<wicri:noCountry code="subField">Hill</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Yamada, Shizuka" sort="Yamada, Shizuka" uniqKey="Yamada S" first="Shizuka" last="Yamada">Shizuka Yamada</name>
<affiliation wicri:level="1"><mods:affiliation>Department of Cariology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Cariology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Azuma, Yoshiaki" sort="Azuma, Yoshiaki" uniqKey="Azuma Y" first="Yoshiaki" last="Azuma">Yoshiaki Azuma</name>
<affiliation wicri:level="1"><mods:affiliation>Teijin Institute for Bio‐Medical Research, Teijin Pharma Ltd., Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Teijin Institute for Bio‐Medical Research, Teijin Pharma Ltd., Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chida, Takayuki" sort="Chida, Takayuki" uniqKey="Chida T" first="Takayuki" last="Chida">Takayuki Chida</name>
<affiliation wicri:level="1"><mods:affiliation>Teijin Institute for Bio‐Medical Research, Teijin Pharma Ltd., Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Teijin Institute for Bio‐Medical Research, Teijin Pharma Ltd., Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yamauchi, Mitsuo" sort="Yamauchi, Mitsuo" uniqKey="Yamauchi M" first="Mitsuo" last="Yamauchi">Mitsuo Yamauchi</name>
<affiliation><mods:affiliation>NC Oral Health Institute, University of North Carolina at Chapel Hill, North Carolina, Chapel Hill</mods:affiliation>
<wicri:noCountry code="subField">Hill</wicri:noCountry>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>E-mail: mitsuo_yamauchi@unc.edu</mods:affiliation>
<country wicri:rule="url">États-Unis</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:966551EB5C792C71E58B588564A40EF5BD182BB6</idno>
<date when="2014" year="2014">2014</date>
<idno type="doi">10.1002/jor.22642</idno>
<idno type="url">https://api.istex.fr/document/966551EB5C792C71E58B588564A40EF5BD182BB6/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">004B10</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">004B10</idno>
<idno type="wicri:Area/Istex/Curation">004B10</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">Alfacalcidol enhances collagen quality in ovariectomized rat bones</title>
<author><name sortKey="Nagaoka, Hideaki" sort="Nagaoka, Hideaki" uniqKey="Nagaoka H" first="Hideaki" last="Nagaoka">Hideaki Nagaoka</name>
<affiliation><mods:affiliation>NC Oral Health Institute, University of North Carolina at Chapel Hill, North Carolina, Chapel Hill</mods:affiliation>
<wicri:noCountry code="subField">Hill</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Terajima, Masahiko" sort="Terajima, Masahiko" uniqKey="Terajima M" first="Masahiko" last="Terajima">Masahiko Terajima</name>
<affiliation><mods:affiliation>NC Oral Health Institute, University of North Carolina at Chapel Hill, North Carolina, Chapel Hill</mods:affiliation>
<wicri:noCountry code="subField">Hill</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Yamada, Shizuka" sort="Yamada, Shizuka" uniqKey="Yamada S" first="Shizuka" last="Yamada">Shizuka Yamada</name>
<affiliation wicri:level="1"><mods:affiliation>Department of Cariology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Cariology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Azuma, Yoshiaki" sort="Azuma, Yoshiaki" uniqKey="Azuma Y" first="Yoshiaki" last="Azuma">Yoshiaki Azuma</name>
<affiliation wicri:level="1"><mods:affiliation>Teijin Institute for Bio‐Medical Research, Teijin Pharma Ltd., Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Teijin Institute for Bio‐Medical Research, Teijin Pharma Ltd., Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Chida, Takayuki" sort="Chida, Takayuki" uniqKey="Chida T" first="Takayuki" last="Chida">Takayuki Chida</name>
<affiliation wicri:level="1"><mods:affiliation>Teijin Institute for Bio‐Medical Research, Teijin Pharma Ltd., Tokyo, Japan</mods:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Teijin Institute for Bio‐Medical Research, Teijin Pharma Ltd., Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yamauchi, Mitsuo" sort="Yamauchi, Mitsuo" uniqKey="Yamauchi M" first="Mitsuo" last="Yamauchi">Mitsuo Yamauchi</name>
<affiliation><mods:affiliation>NC Oral Health Institute, University of North Carolina at Chapel Hill, North Carolina, Chapel Hill</mods:affiliation>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>E-mail: mitsuo_yamauchi@unc.edu</mods:affiliation>
<country wicri:rule="url">États-Unis</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Journal of Orthopaedic Research</title>
<title level="j" type="alt">JOURNAL OF ORTHOPAEDIC RESEARCH</title>
<idno type="ISSN">0736-0266</idno>
<idno type="eISSN">1554-527X</idno>
<imprint><biblScope unit="vol">32</biblScope>
<biblScope unit="issue">8</biblScope>
<biblScope unit="page" from="1030">1030</biblScope>
<biblScope unit="page" to="1036">1036</biblScope>
<biblScope unit="page-count">7</biblScope>
<date type="published" when="2014-08">2014-08</date>
</imprint>
<idno type="ISSN">0736-0266</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0736-0266</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The aim of this study was to investigate the effects of alfacalcidol (1α(OH)D3: ALF) on bone collagen employing an ovariectomized rat model. Thirty‐five 16‐week‐old female Sprague‐Dawley rats were divided into five groups: SHAM (sham‐operated + vehicle), OVX (ovariectomy + vehicle), and three ALF‐treated groups, that is, ovariectomy + 0.022 µg/kg/day ALF, ovariectomy + 0.067 µg/kg/day ALF, and ovariectomy + 0.2 µg/kg/day ALF. After 12 weeks of treatment, tibiae were subjected to histological, biochemical and immunohistochemical analyses. Collagen matrices in OVX bone appeared as immature and poorly organized; however, with the ALF treatment, it was improved in a dose‐dependent manner. Contents of collagen and pyridinoline cross‐link were decreased in OVX compared with SHAM, but they increased to the level comparable to SHAM in ALF‐treated groups. The total aldehyde, that is, a sum of free and those involved cross‐links, in the highest dose of ALF was significantly higher than the rest of the groups (p < 0.05). In addition, the expression of lysyl oxidase was increased in the all ALF‐treated groups compared with OVX (p < 0.05). In conclusion, ALF increases not only the amount of collagen but also enhances the maturation of collagen in ovariectomy‐induced osteoporotic bones, which likely contributes to the improvement of bone quality. © 2014 The Authors. Journal of Orthopaedic Research. Published by Wiley Periodicals, Inc. J Orthop Res 32:1030–1036, 2014.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV2/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004B10 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 004B10 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Santé |area= EdenteV2 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:966551EB5C792C71E58B588564A40EF5BD182BB6 |texte= Alfacalcidol enhances collagen quality in ovariectomized rat bones }}
This area was generated with Dilib version V0.6.32. |