Antibiotic resistance in human peri‐implantitis microbiota
Identifieur interne : 006D58 ( Istex/Corpus ); précédent : 006D57; suivant : 006D59Antibiotic resistance in human peri‐implantitis microbiota
Auteurs : Thomas E. Rams ; John E. Degener ; Arie J. Van WinkelhoffSource :
- Clinical Oral Implants Research [ 0905-7161 ] ; 2014-01.
Abstract
Because antimicrobial therapy is often employed in the treatment of infectious dental implant complications, this study determined the occurrence of in vitro antibiotic resistance among putative peri‐implantitis bacterial pathogens.
Url:
DOI: 10.1111/clr.12160
Links to Exploration step
ISTEX:DC98875019BDD22F7D4E7977DBB095EA5339C98ALe document en format XML
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Rams</name><affiliation><mods:affiliation>Department of Periodontology and Oral Implantology, and Oral Microbiology Testing Service Laboratory, Temple University School of Dentistry, Philadelphia, PA, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Center for Dentistry and Oral Hygiene, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands</mods:affiliation></affiliation><affiliation><mods:affiliation>, DDS, MHSDepartment of Periodontology and Oral Implantology, Temple University School of Dentistry, 3223 North Broad Street, Philadelphia, PA 19140, USATel.: +215 707 2941Fax: +215 707 4223e‐mail:</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: trams@temple.edu</mods:affiliation></affiliation></author><author><name sortKey="Degener, John E" sort="Degener, John E" uniqKey="Degener J" first="John E." last="Degener">John E. 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Rams</name><affiliation><mods:affiliation>Department of Periodontology and Oral Implantology, and Oral Microbiology Testing Service Laboratory, Temple University School of Dentistry, Philadelphia, PA, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Center for Dentistry and Oral Hygiene, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands</mods:affiliation></affiliation><affiliation><mods:affiliation>, DDS, MHSDepartment of Periodontology and Oral Implantology, Temple University School of Dentistry, 3223 North Broad Street, Philadelphia, PA 19140, USATel.: +215 707 2941Fax: +215 707 4223e‐mail:</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: trams@temple.edu</mods:affiliation></affiliation></author><author><name sortKey="Degener, John E" sort="Degener, John E" uniqKey="Degener J" first="John E." last="Degener">John E. 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Van Winkelhoff</name><affiliation><mods:affiliation>Center for Dentistry and Oral Hygiene, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Clinical Oral Implants Research</title><title level="j" type="alt">CLINICAL ORAL IMPLANTS RESEARCH</title><idno type="ISSN">0905-7161</idno><idno type="eISSN">1600-0501</idno><imprint><biblScope unit="vol">25</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="82">82</biblScope><biblScope unit="page" to="90">90</biblScope><biblScope unit="page-count">9</biblScope><date type="published" when="2014-01">2014-01</date></imprint><idno type="ISSN">0905-7161</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0905-7161</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Because antimicrobial therapy is often employed in the treatment of infectious dental implant complications, this study determined the occurrence of in vitro antibiotic resistance among putative peri‐implantitis bacterial pathogens.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Thomas E. Rams</name><affiliations><json:string>Department of Periodontology and Oral Implantology, and Oral Microbiology Testing Service Laboratory, Temple University School of Dentistry, Philadelphia, PA, USA</json:string><json:string>Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA, USA</json:string><json:string>Center for Dentistry and Oral Hygiene, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands</json:string><json:string>, DDS, MHSDepartment of Periodontology and Oral Implantology, Temple University School of Dentistry, 3223 North Broad Street, Philadelphia, PA 19140, USATel.: +215 707 2941Fax: +215 707 4223e‐mail:</json:string><json:string>E-mail: trams@temple.edu</json:string></affiliations></json:item><json:item><name>John E. Degener</name><affiliations><json:string>Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands</json:string></affiliations></json:item><json:item><name>Arie J. van Winkelhoff</name><affiliations><json:string>Center for Dentistry and Oral Hygiene, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands</json:string><json:string>Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>peri‐implantitis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>antibiotic resistance</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>submucosal microbiota</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>in vitro</value></json:item></subject><articleId><json:string>CLR12160</json:string></articleId><arkIstex>ark:/67375/WNG-0SZT4N49-1</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Because antimicrobial therapy is often employed in the treatment of infectious dental implant complications, this study determined the occurrence of in vitro antibiotic resistance among putative peri‐implantitis bacterial pathogens.</abstract><qualityIndicators><score>7.348</score><pdfWordCount>6561</pdfWordCount><pdfCharCount>48359</pdfCharCount><pdfVersion>1.7</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>595.276 x 782.362 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>29</abstractWordCount><abstractCharCount>232</abstractCharCount><keywordCount>4</keywordCount></qualityIndicators><title>Antibiotic resistance in human peri‐implantitis microbiota</title><genre><json:string>article</json:string></genre><host><title>Clinical Oral Implants Research</title><language><json:string>unknown</json:string></language><doi><json:string>10.1111/(ISSN)1600-0501</json:string></doi><issn><json:string>0905-7161</json:string></issn><eissn><json:string>1600-0501</json:string></eissn><publisherId><json:string>CLR</json:string></publisherId><volume>25</volume><issue>1</issue><pages><first>82</first><last>90</last><total>9</total></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Original Article</value></json:item></subject></host><ark><json:string>ark:/67375/WNG-0SZT4N49-1</json:string></ark><publicationDate>2014</publicationDate><copyrightDate>2014</copyrightDate><doi><json:string>10.1111/clr.12160</json:string></doi><id>DC98875019BDD22F7D4E7977DBB095EA5339C98A</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/DC98875019BDD22F7D4E7977DBB095EA5339C98A/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/DC98875019BDD22F7D4E7977DBB095EA5339C98A/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/DC98875019BDD22F7D4E7977DBB095EA5339C98A/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main">Antibiotic resistance in human peri‐implantitis microbiota</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Publishing Ltd</publisher><availability><licence>© 2013 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd</licence></availability><date type="published" when="2014-01"></date></publicationStmt><notesStmt><note type="content-type" subtype="article" source="article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="article"><analytic><title level="a" type="main">Antibiotic resistance in human peri‐implantitis microbiota</title><author xml:id="author-0000" role="corresp"><persName><forename type="first">Thomas E.</forename><surname>Rams</surname></persName><affiliation><orgName>Department of Periodontology and Oral Implantology</orgName><orgName>and Oral Microbiology Testing Service Laboratory</orgName><orgName>Temple University School of Dentistry</orgName><address><settlement type="city">Philadelphia</settlement><region>PA</region><country key="US">USA</country></address></affiliation><affiliation><orgName>Department of Microbiology and Immunology</orgName><orgName>Temple University School of Medicine</orgName><address><settlement type="city">Philadelphia</settlement><region>PA</region><country key="US">USA</country></address></affiliation><affiliation><orgName>Center for Dentistry and Oral Hygiene</orgName><orgName>University Medical Center Groningen</orgName><orgName>University of Groningen</orgName><address><settlement type="city">Groningen</settlement><country key="NL">The Netherlands</country></address></affiliation><affiliation>Corresponding author: Thomas E. Rams, DDS, MHS Department of Periodontology and Oral Implantology, Temple University School of Dentistry, 3223 North Broad Street, Philadelphia, PA 19140, USA Tel.: +215 707 2941 Fax: +215 707 4223 e‐mail: trams@temple.edu</affiliation></author><author xml:id="author-0001"><persName><forename type="first">John E.</forename><surname>Degener</surname></persName><affiliation><orgName>Department of Medical Microbiology</orgName><orgName>University Medical Center Groningen</orgName><orgName>University of Groningen</orgName><address><settlement type="city">Groningen</settlement><country key="NL">The Netherlands</country></address></affiliation></author><author xml:id="author-0002"><persName><forename type="first">Arie J.</forename><surname>Winkelhoff</surname></persName><affiliation><orgName>Center for Dentistry and Oral Hygiene</orgName><orgName>University Medical Center Groningen</orgName><orgName>University of Groningen</orgName><address><settlement type="city">Groningen</settlement><country key="NL">The Netherlands</country></address></affiliation><affiliation><orgName>Department of Medical Microbiology</orgName><orgName>University Medical Center Groningen</orgName><orgName>University of Groningen</orgName><address><settlement type="city">Groningen</settlement><country key="NL">The Netherlands</country></address></affiliation></author><idno type="istex">DC98875019BDD22F7D4E7977DBB095EA5339C98A</idno><idno type="ark">ark:/67375/WNG-0SZT4N49-1</idno><idno type="DOI">10.1111/clr.12160</idno><idno type="unit">CLR12160</idno><idno type="toTypesetVersion">file:CLR.CLR12160.pdf</idno></analytic><monogr><title level="j" type="main">Clinical Oral Implants Research</title><title level="j" type="alt">CLINICAL ORAL IMPLANTS RESEARCH</title><idno type="pISSN">0905-7161</idno><idno type="eISSN">1600-0501</idno><idno type="book-DOI">10.1111/(ISSN)1600-0501</idno><idno type="book-part-DOI">10.1111/clr.2013.25.issue-1</idno><idno type="product">CLR</idno><imprint><biblScope unit="vol">25</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="82">82</biblScope><biblScope unit="page" to="90">90</biblScope><biblScope unit="page-count">9</biblScope><date type="published" when="2014-01"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en" style="main" xml:id="clr12160-abs-0001"><head>Abstract</head>
Objectives
<p>Because antimicrobial therapy is often employed in the treatment of infectious dental implant complications, this study determined the occurrence of <hi rend="italic">in vitro</hi> antibiotic resistance among putative peri‐implantitis bacterial pathogens.</p>
Methods
<p>Submucosal biofilm specimens were cultured from 160 dental implants with peri‐implantitis in 120 adults, with isolated putative pathogens identified to species level, and tested <hi rend="italic">in vitro</hi> for susceptibility to 4 mg/l of doxycycline, 8 mg/l of amoxicillin, 16 mg/l of metronidazole, and 4 mg/l of clindamycin. Findings for amoxicillin and metronidazole were combined <hi rend="italic">post‐hoc</hi> to identify peri‐implantitis species resistant to both antibiotics. Gram‐negative enteric rods/pseudomonads were subjected to ciprofloxacin disk diffusion testing.</p>
Results
<p>One or more cultivable submucosal bacterial pathogens, most often <hi rend="italic"><hi rend="fc">P</hi>revotella intermedia</hi>/<hi rend="italic">nigrescens</hi> or <hi rend="italic"><hi rend="fc">S</hi>treptococcus constellatus</hi>, were resistant <hi rend="italic">in vitro</hi> to clindamycin, amoxicillin, doxycycline, or metronidazole in 46.7%, 39.2%, 25%, and 21.7% of the peri‐implantitis subjects, respectively. Only 6.7% subjects revealed submucosal test species resistant <hi rend="italic">in vitro</hi> to both amoxicillin and metronidazole, which were either <hi rend="italic"><hi rend="fc">S</hi>. constellatus</hi> (one subject) or ciprofloxacin‐susceptible strains of gram‐negative enteric rods/pseudomonads (seven subjects). Overall, 71.7% of the 120 peri‐implantitis subjects exhibited submucosal bacterial pathogens resistant <hi rend="italic">in vitro</hi> to one or more of the tested antibiotics.</p>
Conclusions
<p>Peri‐implantitis patients frequently yielded submucosal bacterial pathogens resistant <hi rend="italic">in vitro</hi> to individual therapeutic concentrations of clindamycin, amoxicillin, doxycycline, or metronidazole, but only rarely to both amoxicillin and metronidazole. Due to the wide variation in observed drug resistance patterns, antibiotic susceptibility testing of cultivable submucosal bacterial pathogens may aid in the selection of antimicrobial therapy for peri‐implantitis patients.</p></abstract><textClass><keywords><term xml:id="clr12160-kwd-0001">peri‐implantitis</term><term xml:id="clr12160-kwd-0002">antibiotic resistance</term><term xml:id="clr12160-kwd-0003">submucosal microbiota</term><term xml:id="clr12160-kwd-0004"><hi rend="italic">in vitro</hi></term></keywords><keywords rend="articleCategory"><term>Original Article</term></keywords><keywords rend="tocHeading1"><term>Original Articles</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component type="serialArticle" version="2.0" xml:id="clr12160" xml:lang="en"><header><publicationMeta level="product"><doi origin="wiley" registered="yes">10.1111/(ISSN)1600-0501</doi><issn type="print">0905-7161</issn><issn type="electronic">1600-0501</issn><idGroup><id type="product" value="CLR"></id></idGroup><titleGroup><title sort="CLINICAL ORAL IMPLANTS RESEARCH" type="main">Clinical Oral Implants Research</title><title type="short">Clin. Oral Impl. Res.</title></titleGroup></publicationMeta><publicationMeta level="part" position="10"><doi origin="wiley">10.1111/clr.2013.25.issue-1</doi><copyright ownership="publisher">Copyright © 2014 John Wiley & Sons Ltd</copyright><numberingGroup><numbering type="journalVolume" number="25">25</numbering><numbering type="journalIssue">1</numbering></numberingGroup><coverDate startDate="2014-01">January 2014</coverDate></publicationMeta><publicationMeta level="unit" position="140" status="forIssue" type="article"><doi>10.1111/clr.12160</doi><idGroup><id type="unit" value="CLR12160"></id></idGroup><countGroup><count number="9" type="pageTotal"></count></countGroup><titleGroup><title type="articleCategory">Original Article</title><title type="tocHeading1">Original Articles</title></titleGroup><copyright ownership="publisher">© 2013 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd</copyright><eventGroup><event date="2013-02-26" type="manuscriptAccepted"></event><event agent="SPS" date="2013-03-12" type="xmlCreated"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.2.7 mode:FullText" date="2013-12-17"></event><event type="publishedOnlineEarlyUnpaginated" date="2013-04-02"></event><event type="firstOnline" date="2013-04-02"></event><event type="publishedOnlineFinalForm" date="2013-12-17"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.6.4 mode:FullText" date="2015-10-08"></event></eventGroup><numberingGroup><numbering type="pageFirst">82</numbering><numbering type="pageLast">90</numbering></numberingGroup><correspondenceTo><lineatedText><line><b>Corresponding author:</b></line><line><i>Thomas E. Rams</i>, DDS, MHS</line><line>Department of Periodontology and Oral Implantology, Temple University School of Dentistry, 3223 North Broad Street, Philadelphia, PA 19140, USA</line><line>Tel.: +215 707 2941</line><line>Fax: +215 707 4223</line><line>e‐mail: <email>trams@temple.edu</email></line></lineatedText></correspondenceTo><selfCitationGroup><citation type="self" xml:id="clr12160-cit-1001"><author><familyName>Rams</familyName> <givenNames>TE</givenNames></author>, <author><familyName>Degener</familyName> <givenNames>JE</givenNames></author>, <author><familyNamePrefix>van</familyNamePrefix> <familyName>Winkelhoff</familyName> <givenNames>AJ</givenNames></author>. <articleTitle>Antibiotic resistance in human peri‐implantitis microbiota</articleTitle>. <journalTitle>Clin. Oral Impl. Res.</journalTitle> <vol>25</vol>, <pubYear year="2014">2014</pubYear>, <pageFirst>82</pageFirst>–<pageLast>90</pageLast></citation></selfCitationGroup><linkGroup><link type="toTypesetVersion" href="file:CLR.CLR12160.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Antibiotic resistance in human peri‐implantitis microbiota</title><title type="shortAuthors">Rams et al</title></titleGroup><creators><creator affiliationRef="#clr12160-aff-0001 #clr12160-aff-0002 #clr12160-aff-0003" corresponding="yes" creatorRole="author" xml:id="clr12160-cr-0001"><personName><givenNames>Thomas E.</givenNames> <familyName>Rams</familyName></personName></creator><creator affiliationRef="#clr12160-aff-0004" creatorRole="author" xml:id="clr12160-cr-0002"><personName><givenNames>John E.</givenNames> <familyName>Degener</familyName></personName></creator><creator affiliationRef="#clr12160-aff-0003 #clr12160-aff-0004" creatorRole="author" xml:id="clr12160-cr-0003"><personName><givenNames>Arie J.</givenNames> <familyNamePrefix>van</familyNamePrefix> <familyName>Winkelhoff</familyName></personName></creator></creators><affiliationGroup><affiliation countryCode="US" type="organization" xml:id="clr12160-aff-0001"> <orgDiv>Department of Periodontology and Oral Implantology</orgDiv> <orgDiv>and Oral Microbiology Testing Service Laboratory</orgDiv> <orgName>Temple University School of Dentistry</orgName> <address><city>Philadelphia</city> <countryPart>PA</countryPart> <country>USA</country></address></affiliation><affiliation countryCode="US" type="organization" xml:id="clr12160-aff-0002"><orgDiv>Department of Microbiology and Immunology</orgDiv> <orgName>Temple University School of Medicine</orgName> <address><city>Philadelphia</city> <countryPart>PA</countryPart> <country>USA</country></address></affiliation><affiliation countryCode="NL" type="organization" xml:id="clr12160-aff-0003"><orgDiv>Center for Dentistry and Oral Hygiene</orgDiv> <orgName>University Medical Center Groningen</orgName> <orgName>University of Groningen</orgName> <address><city>Groningen</city> <country>The Netherlands</country></address></affiliation><affiliation countryCode="NL" type="organization" xml:id="clr12160-aff-0004"><orgDiv>Department of Medical Microbiology</orgDiv> <orgName>University Medical Center Groningen</orgName> <orgName>University of Groningen</orgName> <address><city>Groningen</city> <country>The Netherlands</country></address></affiliation></affiliationGroup><keywordGroup type="author"><keyword xml:id="clr12160-kwd-0001">peri‐implantitis</keyword><keyword xml:id="clr12160-kwd-0002">antibiotic resistance</keyword><keyword xml:id="clr12160-kwd-0003">submucosal microbiota</keyword><keyword xml:id="clr12160-kwd-0004"><i>in vitro</i></keyword></keywordGroup><fundingInfo><fundingAgency>Temple University School of Dentistry</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:id="clr12160-abs-0001" xml:lang="en"><title type="main">Abstract</title><section xml:id="clr12160-sec-0001"><title type="main">Objectives</title><p>Because antimicrobial therapy is often employed in the treatment of infectious dental implant complications, this study determined the occurrence of <i>in vitro</i> antibiotic resistance among putative peri‐implantitis bacterial pathogens.</p></section><section xml:id="clr12160-sec-0002"><title type="main">Methods</title><p>Submucosal biofilm specimens were cultured from 160 dental implants with peri‐implantitis in 120 adults, with isolated putative pathogens identified to species level, and tested <i>in vitro</i> for susceptibility to 4 mg/l of doxycycline, 8 mg/l of amoxicillin, 16 mg/l of metronidazole, and 4 mg/l of clindamycin. Findings for amoxicillin and metronidazole were combined <i>post‐hoc</i> to identify peri‐implantitis species resistant to both antibiotics. Gram‐negative enteric rods/pseudomonads were subjected to ciprofloxacin disk diffusion testing.</p></section><section xml:id="clr12160-sec-0003"><title type="main">Results</title><p>One or more cultivable submucosal bacterial pathogens, most often <i><fc>P</fc>revotella intermedia</i>/<i>nigrescens</i> or <i><fc>S</fc>treptococcus constellatus</i>, were resistant <i>in vitro</i> to clindamycin, amoxicillin, doxycycline, or metronidazole in 46.7%, 39.2%, 25%, and 21.7% of the peri‐implantitis subjects, respectively. Only 6.7% subjects revealed submucosal test species resistant <i>in vitro</i> to both amoxicillin and metronidazole, which were either <i><fc>S</fc>. constellatus</i> (one subject) or ciprofloxacin‐susceptible strains of gram‐negative enteric rods/pseudomonads (seven subjects). Overall, 71.7% of the 120 peri‐implantitis subjects exhibited submucosal bacterial pathogens resistant <i>in vitro</i> to one or more of the tested antibiotics.</p></section><section xml:id="clr12160-sec-0004"><title type="main">Conclusions</title><p>Peri‐implantitis patients frequently yielded submucosal bacterial pathogens resistant <i>in vitro</i> to individual therapeutic concentrations of clindamycin, amoxicillin, doxycycline, or metronidazole, but only rarely to both amoxicillin and metronidazole. Due to the wide variation in observed drug resistance patterns, antibiotic susceptibility testing of cultivable submucosal bacterial pathogens may aid in the selection of antimicrobial therapy for peri‐implantitis patients.</p></section></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Antibiotic resistance in human peri‐implantitis microbiota</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Antibiotic resistance in human peri‐implantitis microbiota</title></titleInfo><name type="personal"><namePart type="given">Thomas E.</namePart><namePart type="family">Rams</namePart><affiliation>Department of Periodontology and Oral Implantology, and Oral Microbiology Testing Service Laboratory, Temple University School of Dentistry, Philadelphia, PA, USA</affiliation><affiliation>Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA, USA</affiliation><affiliation>Center for Dentistry and Oral Hygiene, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands</affiliation><affiliation>, DDS, MHSDepartment of Periodontology and Oral Implantology, Temple University School of Dentistry, 3223 North Broad Street, Philadelphia, PA 19140, USATel.: +215 707 2941Fax: +215 707 4223e‐mail:</affiliation><affiliation>E-mail: trams@temple.edu</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">John E.</namePart><namePart type="family">Degener</namePart><affiliation>Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Arie J.</namePart><namePart type="family">van Winkelhoff</namePart><affiliation>Center for Dentistry and Oral Hygiene, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands</affiliation><affiliation>Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><dateIssued encoding="w3cdtf">2014-01</dateIssued><dateCreated encoding="w3cdtf">2013-03-12</dateCreated><dateValid encoding="w3cdtf">2013-02-26</dateValid><edition>Rams TE, Degener JE, van Winkelhoff AJ. Antibiotic resistance in human peri‐implantitis microbiota. Clin. Oral Impl. Res. 25, 2014, 82–90</edition><copyrightDate encoding="w3cdtf">2014</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract>Because antimicrobial therapy is often employed in the treatment of infectious dental implant complications, this study determined the occurrence of in vitro antibiotic resistance among putative peri‐implantitis bacterial pathogens.</abstract><abstract>Submucosal biofilm specimens were cultured from 160 dental implants with peri‐implantitis in 120 adults, with isolated putative pathogens identified to species level, and tested in vitro for susceptibility to 4 mg/l of doxycycline, 8 mg/l of amoxicillin, 16 mg/l of metronidazole, and 4 mg/l of clindamycin. Findings for amoxicillin and metronidazole were combined post‐hoc to identify peri‐implantitis species resistant to both antibiotics. Gram‐negative enteric rods/pseudomonads were subjected to ciprofloxacin disk diffusion testing.</abstract><abstract>One or more cultivable submucosal bacterial pathogens, most often Prevotella intermedia/nigrescens or Streptococcus constellatus, were resistant in vitro to clindamycin, amoxicillin, doxycycline, or metronidazole in 46.7%, 39.2%, 25%, and 21.7% of the peri‐implantitis subjects, respectively. Only 6.7% subjects revealed submucosal test species resistant in vitro to both amoxicillin and metronidazole, which were either S. constellatus (one subject) or ciprofloxacin‐susceptible strains of gram‐negative enteric rods/pseudomonads (seven subjects). Overall, 71.7% of the 120 peri‐implantitis subjects exhibited submucosal bacterial pathogens resistant in vitro to one or more of the tested antibiotics.</abstract><abstract>Peri‐implantitis patients frequently yielded submucosal bacterial pathogens resistant in vitro to individual therapeutic concentrations of clindamycin, amoxicillin, doxycycline, or metronidazole, but only rarely to both amoxicillin and metronidazole. Due to the wide variation in observed drug resistance patterns, antibiotic susceptibility testing of cultivable submucosal bacterial pathogens may aid in the selection of antimicrobial therapy for peri‐implantitis patients.</abstract><note type="funding">Temple University School of Dentistry</note><subject><genre>keywords</genre><topic>peri‐implantitis</topic><topic>antibiotic resistance</topic><topic>submucosal microbiota</topic><topic>in vitro</topic></subject><relatedItem type="host"><titleInfo><title>Clinical Oral Implants Research</title></titleInfo><titleInfo type="abbreviated"><title>Clin. Oral Impl. Res.</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><subject><genre>article-category</genre><topic>Original Article</topic></subject><identifier type="ISSN">0905-7161</identifier><identifier type="eISSN">1600-0501</identifier><identifier type="DOI">10.1111/(ISSN)1600-0501</identifier><identifier type="PublisherID">CLR</identifier><part><date>2014</date><detail type="volume"><caption>vol.</caption><number>25</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>82</start><end>90</end><total>9</total></extent></part></relatedItem><identifier type="istex">DC98875019BDD22F7D4E7977DBB095EA5339C98A</identifier><identifier type="ark">ark:/67375/WNG-0SZT4N49-1</identifier><identifier type="DOI">10.1111/clr.12160</identifier><identifier type="ArticleID">CLR12160</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2014 John Wiley & Sons Ltd© 2013 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/DC98875019BDD22F7D4E7977DBB095EA5339C98A/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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