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Bacterial leakage along the implant–abutment interface: culture and DNA Checkerboard hybridization analyses

Identifieur interne : 006016 ( Istex/Corpus ); précédent : 006015; suivant : 006017

Bacterial leakage along the implant–abutment interface: culture and DNA Checkerboard hybridization analyses

Auteurs : Cássio Do Nascimento ; Paola Kirsten Miani ; Vinícius Pedrazzi ; Kátia Muller ; Rubens Ferreira De Albuquerque Junior

Source :

RBID : ISTEX:C141201F09F70F2D6F857965156EA8A273BDEAEB

English descriptors

Abstract

Bacterial species have been found harboring the internal surface of dental implants as consequence of their failed connections. The aim of the present study was to compare the detection frequency of bacterial leakage from human saliva through the implant–abutment interface, under non‐loading conditions, using either DNA Checkerboard or culture method.

Url:
DOI: 10.1111/j.1600-0501.2011.02280.x

Links to Exploration step

ISTEX:C141201F09F70F2D6F857965156EA8A273BDEAEB

Le document en format XML

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<div type="abstract">Bacterial species have been found harboring the internal surface of dental implants as consequence of their failed connections. The aim of the present study was to compare the detection frequency of bacterial leakage from human saliva through the implant–abutment interface, under non‐loading conditions, using either DNA Checkerboard or culture method.</div>
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Objective
<p>Bacterial species have been found harboring the internal surface of dental implants as consequence of their failed connections. The aim of the present study was to compare the detection frequency of bacterial leakage from human saliva through the implant–abutment interface, under non‐loading conditions, using either
<hi rend="fc">DNA</hi>
Checkerboard or culture method.</p>
Materials and methods
<p>Thirty dental implants with hexagonal platforms were connected to pre‐machined abutments according to the manufacturers’ specifications. The assemblies were individually incubated in human saliva under anaerobic conditions for 7 days at 37°C. Afterward, contents from the inner parts of the implants were collected and evaluated with either
<hi rend="fc">DNA</hi>
Checkerboard (
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Results
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<hi rend="italic">C</hi>
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<hi rend="italic">apnocytophaga gingivalis</hi>
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<hi rend="fc">DNA</hi>
Checkerboard hybridization and conventional culture methods.</p>
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<p>Bacterial species have been found harboring the internal surface of dental implants as consequence of their failed connections. The aim of the present study was to compare the detection frequency of bacterial leakage from human saliva through the implant–abutment interface, under non‐loading conditions, using either
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<p>Thirty dental implants with hexagonal platforms were connected to pre‐machined abutments according to the manufacturers’ specifications. The assemblies were individually incubated in human saliva under anaerobic conditions for 7 days at 37°C. Afterward, contents from the inner parts of the implants were collected and evaluated with either
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