Blastogenic responsiveness of human lymphoid cells to mitogens and to homogenates of periodontal pocket bacteria
Identifieur interne : 003A79 ( Istex/Corpus ); précédent : 003A78; suivant : 003A80Blastogenic responsiveness of human lymphoid cells to mitogens and to homogenates of periodontal pocket bacteria
Auteurs : Jon B. Suzuki ; Tom J. Sims ; David L. Singer ; Roy C. PageSource :
- Journal of Periodontal Research [ 0022-3484 ] ; 1984-07.
English descriptors
- KwdEn :
- Actinomyces viscosus, Activator, Activator concentrations, Activator dose, American academy, Amlr, Assay system, Autologous, Bacterial homogenates, Bacterial preparations, Bacterial substances, Blastogenesis, Blastogenic, Blastogenic response, Blastogenic responsiveness, Bone loss score, Cell concentration, Cell concentrations, Chronic gingivitis, Clinical immunology, Control cells, Control donors, Control subject, Control subjects, Conventional antigen, Culture conditions, Experimental medicine, Greatest extent, Human autologous, Human lymphocytes, Human periodonta, Immunology, Incorporation, Incubation time, Incubation times, Ivanyi lehner, Johns hopkins hospital, Juvenile periodontitis, Label incorporation, Lymphocyte, Lymphocyte reaction, Lymphocyte reactions, Lymphocyte transformation, Maximally, Mitogen, Oral biology, Patient cells, Patient cultures, Pediatric dentistry, Periodontal, Periodontal disease, Periodontal research, Periodontal status, Periodontitis, Peripheral blood, Peripheral blood lymphocytes, Plaque antigens, Pokeweed mitogen, Precursor, Radiographic measurements, Responsiveness, Severe periodontitis, Significant differences, Sims, Sims page, Smith lang, Spontaneous lymphocyte proliferation, Stimulation index, Suzuki, Test conditions, Unstimulated, Unstimulated cultures, Young adults.
- Teeft :
- Actinomyces viscosus, Activator, Activator concentrations, Activator dose, American academy, Amlr, Assay system, Autologous, Bacterial homogenates, Bacterial preparations, Bacterial substances, Blastogenesis, Blastogenic, Blastogenic response, Blastogenic responsiveness, Bone loss score, Cell concentration, Cell concentrations, Chronic gingivitis, Clinical immunology, Control cells, Control donors, Control subject, Control subjects, Conventional antigen, Culture conditions, Experimental medicine, Greatest extent, Human autologous, Human lymphocytes, Human periodonta, Immunology, Incorporation, Incubation time, Incubation times, Ivanyi lehner, Johns hopkins hospital, Juvenile periodontitis, Label incorporation, Lymphocyte, Lymphocyte reaction, Lymphocyte reactions, Lymphocyte transformation, Maximally, Mitogen, Oral biology, Patient cells, Patient cultures, Pediatric dentistry, Periodontal, Periodontal disease, Periodontal research, Periodontal status, Periodontitis, Peripheral blood, Peripheral blood lymphocytes, Plaque antigens, Pokeweed mitogen, Precursor, Radiographic measurements, Responsiveness, Severe periodontitis, Significant differences, Sims, Sims page, Smith lang, Spontaneous lymphocyte proliferation, Stimulation index, Suzuki, Test conditions, Unstimulated, Unstimulated cultures, Young adults.
Abstract
Experiments aimed at demonstrating significant differences in the blastogenic responsiveness of peripheral blood mononuclear cells (PBM) activated using bacterial preparations and pokeweed mitogen (PWM) were performed. The known variables in the in vitro assay system were controlled to the greatest extent possible. A range of cell and activator concentrations, incubation times extending from 3 to 7 days, conical microtest wells, and a labeling procedure which accurately reflects blastogenesis was used. Under these test conditions, cultures of activated patient cells did not differ significantly from activated control cells in counts incorporated, in Stimulation Index, or in incubation time, cell concentration, or activator dose required for maximal blastogenesis, except for cultures activated with LPS and PWM. Responsiveness of cultures of patient cells activated with LPS was significantly lower than that of cultures of control cells (P < 0.01). Cultures from most normal control donors exposed to PWM became maximally activated at 3 days' incubation, while cultures from most patients required 5 to 7 days. Cultures of patient cells did differ significantly (p < 0.05) from control cells in that their spontaneous lymphocyte proliferation was suppressed. These observations indicate that patients with periodontitis may have abnormalities in basic immunologic control mechanisms.
Url:
DOI: 10.1111/j.1600-0765.1984.tb01008.x
Links to Exploration step
ISTEX:76FE5864E492C71C28772616A5F2E9FCCB2BA0FCLe document en format XML
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Suzuki</name><affiliation><mods:affiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</mods:affiliation></affiliation></author><author><name sortKey="Sims, Tom J" sort="Sims, Tom J" uniqKey="Sims T" first="Tom J." last="Sims">Tom J. Sims</name><affiliation><mods:affiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</mods:affiliation></affiliation></author><author><name sortKey="Singer, David L" sort="Singer, David L" uniqKey="Singer D" first="David L." last="Singer">David L. Singer</name><affiliation><mods:affiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</mods:affiliation></affiliation></author><author><name sortKey="Page, Roy C" sort="Page, Roy C" uniqKey="Page R" first="Roy C." last="Page">Roy C. Page</name><affiliation><mods:affiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Periodontal Research</title><title level="j" type="alt">JOURNAL OF PERIODONTAL RESEARCH</title><idno type="ISSN">0022-3484</idno><idno type="eISSN">1600-0765</idno><imprint><biblScope unit="vol">19</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="352">352</biblScope><biblScope unit="page" to="365">365</biblScope><biblScope unit="page-count">14</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1984-07">1984-07</date></imprint><idno type="ISSN">0022-3484</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-3484</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actinomyces viscosus</term><term>Activator</term><term>Activator concentrations</term><term>Activator dose</term><term>American academy</term><term>Amlr</term><term>Assay system</term><term>Autologous</term><term>Bacterial homogenates</term><term>Bacterial preparations</term><term>Bacterial substances</term><term>Blastogenesis</term><term>Blastogenic</term><term>Blastogenic response</term><term>Blastogenic responsiveness</term><term>Bone loss score</term><term>Cell concentration</term><term>Cell concentrations</term><term>Chronic gingivitis</term><term>Clinical immunology</term><term>Control cells</term><term>Control donors</term><term>Control subject</term><term>Control subjects</term><term>Conventional antigen</term><term>Culture conditions</term><term>Experimental medicine</term><term>Greatest extent</term><term>Human autologous</term><term>Human lymphocytes</term><term>Human periodonta</term><term>Immunology</term><term>Incorporation</term><term>Incubation time</term><term>Incubation times</term><term>Ivanyi lehner</term><term>Johns hopkins hospital</term><term>Juvenile periodontitis</term><term>Label incorporation</term><term>Lymphocyte</term><term>Lymphocyte reaction</term><term>Lymphocyte reactions</term><term>Lymphocyte transformation</term><term>Maximally</term><term>Mitogen</term><term>Oral biology</term><term>Patient cells</term><term>Patient cultures</term><term>Pediatric dentistry</term><term>Periodontal</term><term>Periodontal disease</term><term>Periodontal research</term><term>Periodontal status</term><term>Periodontitis</term><term>Peripheral blood</term><term>Peripheral blood lymphocytes</term><term>Plaque antigens</term><term>Pokeweed mitogen</term><term>Precursor</term><term>Radiographic measurements</term><term>Responsiveness</term><term>Severe periodontitis</term><term>Significant differences</term><term>Sims</term><term>Sims page</term><term>Smith lang</term><term>Spontaneous lymphocyte proliferation</term><term>Stimulation index</term><term>Suzuki</term><term>Test conditions</term><term>Unstimulated</term><term>Unstimulated cultures</term><term>Young adults</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Actinomyces viscosus</term><term>Activator</term><term>Activator concentrations</term><term>Activator dose</term><term>American academy</term><term>Amlr</term><term>Assay system</term><term>Autologous</term><term>Bacterial homogenates</term><term>Bacterial preparations</term><term>Bacterial substances</term><term>Blastogenesis</term><term>Blastogenic</term><term>Blastogenic response</term><term>Blastogenic responsiveness</term><term>Bone loss score</term><term>Cell concentration</term><term>Cell concentrations</term><term>Chronic gingivitis</term><term>Clinical immunology</term><term>Control cells</term><term>Control donors</term><term>Control subject</term><term>Control subjects</term><term>Conventional antigen</term><term>Culture conditions</term><term>Experimental medicine</term><term>Greatest extent</term><term>Human autologous</term><term>Human lymphocytes</term><term>Human periodonta</term><term>Immunology</term><term>Incorporation</term><term>Incubation time</term><term>Incubation times</term><term>Ivanyi lehner</term><term>Johns hopkins hospital</term><term>Juvenile periodontitis</term><term>Label incorporation</term><term>Lymphocyte</term><term>Lymphocyte reaction</term><term>Lymphocyte reactions</term><term>Lymphocyte transformation</term><term>Maximally</term><term>Mitogen</term><term>Oral biology</term><term>Patient cells</term><term>Patient cultures</term><term>Pediatric dentistry</term><term>Periodontal</term><term>Periodontal disease</term><term>Periodontal research</term><term>Periodontal status</term><term>Periodontitis</term><term>Peripheral blood</term><term>Peripheral blood lymphocytes</term><term>Plaque antigens</term><term>Pokeweed mitogen</term><term>Precursor</term><term>Radiographic measurements</term><term>Responsiveness</term><term>Severe periodontitis</term><term>Significant differences</term><term>Sims</term><term>Sims page</term><term>Smith lang</term><term>Spontaneous lymphocyte proliferation</term><term>Stimulation index</term><term>Suzuki</term><term>Test conditions</term><term>Unstimulated</term><term>Unstimulated cultures</term><term>Young adults</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Experiments aimed at demonstrating significant differences in the blastogenic responsiveness of peripheral blood mononuclear cells (PBM) activated using bacterial preparations and pokeweed mitogen (PWM) were performed. The known variables in the in vitro assay system were controlled to the greatest extent possible. A range of cell and activator concentrations, incubation times extending from 3 to 7 days, conical microtest wells, and a labeling procedure which accurately reflects blastogenesis was used. Under these test conditions, cultures of activated patient cells did not differ significantly from activated control cells in counts incorporated, in Stimulation Index, or in incubation time, cell concentration, or activator dose required for maximal blastogenesis, except for cultures activated with LPS and PWM. Responsiveness of cultures of patient cells activated with LPS was significantly lower than that of cultures of control cells (P < 0.01). Cultures from most normal control donors exposed to PWM became maximally activated at 3 days' incubation, while cultures from most patients required 5 to 7 days. Cultures of patient cells did differ significantly (p < 0.05) from control cells in that their spontaneous lymphocyte proliferation was suppressed. These observations indicate that patients with periodontitis may have abnormalities in basic immunologic control mechanisms.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>lymphocyte</json:string><json:string>periodontitis</json:string><json:string>periodontal</json:string><json:string>blastogenic</json:string><json:string>activator</json:string><json:string>autologous</json:string><json:string>immunology</json:string><json:string>mitogen</json:string><json:string>suzuki</json:string><json:string>blastogenesis</json:string><json:string>patient cells</json:string><json:string>amlr</json:string><json:string>unstimulated</json:string><json:string>maximally</json:string><json:string>control subjects</json:string><json:string>control cells</json:string><json:string>cell concentration</json:string><json:string>periodontal disease</json:string><json:string>precursor</json:string><json:string>spontaneous lymphocyte proliferation</json:string><json:string>blastogenic responsiveness</json:string><json:string>sims page</json:string><json:string>incubation time</json:string><json:string>bacterial preparations</json:string><json:string>activator dose</json:string><json:string>lymphocyte reaction</json:string><json:string>blastogenic response</json:string><json:string>experimental medicine</json:string><json:string>significant differences</json:string><json:string>stimulation index</json:string><json:string>pokeweed mitogen</json:string><json:string>bacterial homogenates</json:string><json:string>bacterial substances</json:string><json:string>young adults</json:string><json:string>actinomyces viscosus</json:string><json:string>clinical immunology</json:string><json:string>periodontal research</json:string><json:string>peripheral blood</json:string><json:string>unstimulated cultures</json:string><json:string>oral biology</json:string><json:string>incorporation</json:string><json:string>sims</json:string><json:string>control subject</json:string><json:string>label incorporation</json:string><json:string>american academy</json:string><json:string>ivanyi lehner</json:string><json:string>chronic gingivitis</json:string><json:string>radiographic measurements</json:string><json:string>bone loss score</json:string><json:string>control donors</json:string><json:string>patient cultures</json:string><json:string>cell concentrations</json:string><json:string>periodontal status</json:string><json:string>test conditions</json:string><json:string>incubation times</json:string><json:string>activator concentrations</json:string><json:string>culture conditions</json:string><json:string>greatest extent</json:string><json:string>conventional antigen</json:string><json:string>pediatric dentistry</json:string><json:string>assay system</json:string><json:string>johns hopkins hospital</json:string><json:string>lymphocyte reactions</json:string><json:string>smith lang</json:string><json:string>human autologous</json:string><json:string>lymphocyte transformation</json:string><json:string>peripheral blood lymphocytes</json:string><json:string>juvenile periodontitis</json:string><json:string>human periodonta</json:string><json:string>plaque antigens</json:string><json:string>human lymphocytes</json:string><json:string>severe periodontitis</json:string><json:string>responsiveness</json:string></teeft></keywords><author><json:item><name>Jon B. Suzuki</name><affiliations><json:string>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</json:string><json:string>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</json:string></affiliations></json:item><json:item><name>Tom J. Sims</name><affiliations><json:string>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</json:string><json:string>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</json:string></affiliations></json:item><json:item><name>David L. Singer</name><affiliations><json:string>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</json:string><json:string>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</json:string></affiliations></json:item><json:item><name>Roy C. Page</name><affiliations><json:string>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</json:string><json:string>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</json:string></affiliations></json:item></author><articleId><json:string>JRE352</json:string></articleId><arkIstex>ark:/67375/WNG-VRPC13VV-S</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Experiments aimed at demonstrating significant differences in the blastogenic responsiveness of peripheral blood mononuclear cells (PBM) activated using bacterial preparations and pokeweed mitogen (PWM) were performed. The known variables in the in vitro assay system were controlled to the greatest extent possible. A range of cell and activator concentrations, incubation times extending from 3 to 7 days, conical microtest wells, and a labeling procedure which accurately reflects blastogenesis was used. Under these test conditions, cultures of activated patient cells did not differ significantly from activated control cells in counts incorporated, in Stimulation Index, or in incubation time, cell concentration, or activator dose required for maximal blastogenesis, except for cultures activated with LPS and PWM. Responsiveness of cultures of patient cells activated with LPS was significantly lower than that of cultures of control cells (P > 0.01). Cultures from most normal control donors exposed to PWM became maximally activated at 3 days' incubation, while cultures from most patients required 5 to 7 days. Cultures of patient cells did differ significantly (p > 0.05) from control cells in that their spontaneous lymphocyte proliferation was suppressed. These observations indicate that patients with periodontitis may have abnormalities in basic immunologic control mechanisms.</abstract><qualityIndicators><score>8.988</score><pdfWordCount>4612</pdfWordCount><pdfCharCount>29866</pdfCharCount><pdfVersion>1.6</pdfVersion><pdfPageCount>15</pdfPageCount><pdfPageSize>444 x 671 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>198</abstractWordCount><abstractCharCount>1394</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Blastogenic responsiveness of human lymphoid cells to mitogens and to homogenates of periodontal pocket bacteria</title><pmid><json:string>6235350</json:string></pmid><genre><json:string>article</json:string></genre><host><title>Journal of Periodontal Research</title><language><json:string>unknown</json:string></language><doi><json:string>10.1111/(ISSN)1600-0765</json:string></doi><issn><json:string>0022-3484</json:string></issn><eissn><json:string>1600-0765</json:string></eissn><publisherId><json:string>JRE</json:string></publisherId><volume>19</volume><issue>4</issue><pages><first>352</first><last>365</last><total>14</total></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1984</json:string><json:string>11S</json:string></date><geogName></geogName><orgName><json:string>TjO O CO</json:string><json:string>CO CO</json:string><json:string>Difco Laboratories, Detroit</json:string><json:string>Department of Periodontics, University of Washington, Seattle</json:string><json:string>American Academy of Periodontology</json:string><json:string>Johns Hopkins Hospital, Baltimore, Maryland</json:string><json:string>University of Maryland</json:string><json:string>Johns Hopkins Hospital, Baltimore, Experimental</json:string><json:string>University of Maryland Dental School Drew Award</json:string><json:string>Flow Laboratories</json:string><json:string>Department of Microbiology, University of Maryland, Baltimore</json:string><json:string>University of Washington</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Tim Meiller</json:string><json:string>Camille Sommerville</json:string><json:string>Steve Martin</json:string><json:string>Kathleen Kula</json:string><json:string>Betsy Williams</json:string><json:string>Stefan Levin</json:string><json:string>William Falkler</json:string><json:string>The</json:string><json:string>U.S.A. Experiments</json:string><json:string>Patient</json:string><json:string>Fellowship Award</json:string><json:string>Charles Craig</json:string><json:string>Laurel Risom</json:string><json:string>Searle Model</json:string><json:string>Donald Tilghman</json:string></persName><placeName><json:string>Germany</json:string><json:string>Seattle</json:string><json:string>Baltimore</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Page et al. 1978</json:string><json:string>Sims & Page 1983a, 1983b</json:string><json:string>Ivanyi & Lehner 1970</json:string><json:string>Ranney et al. (1981)</json:string><json:string>Kuntz et al. 1976</json:string><json:string>Patters et al. 1976, 1977</json:string><json:string>Horton et al. 1973</json:string><json:string>Opelz et al. 1975</json:string><json:string>Suzuki et al. 1983</json:string><json:string>Falkler et al. 1982</json:string><json:string>Engelman et al. 1980</json:string><json:string>Suzuki et al. 1982</json:string><json:string>Suzuki et al 1983</json:string><json:string>Beale et al. 1979</json:string><json:string>Hausman et al. 1980</json:string><json:string>Horton et al. (1972)</json:string><json:string>Loe & Silness 1963</json:string><json:string>Sims & Page 1983b</json:string><json:string>Schei et al. (1959)</json:string><json:string>Osterberg et al. (1983)</json:string><json:string>Sims and Page (1983b)</json:string><json:string>Ranney et al. 1981</json:string><json:string>Mackler et al. 1974</json:string><json:string>Accepted for publication February S, 1984</json:string><json:string>Osterberg et al. 1983</json:string><json:string>Sims & Page 1983a</json:string><json:string>Van de Stouwe et al. 1977</json:string><json:string>Baker et al. 1976</json:string><json:string>Suzuki et al. (1982,1983)</json:string><json:string>Patters et al. 1976</json:string><json:string>Moody et al. 1979</json:string><json:string>Suzuki et al. 1982, 1983</json:string><json:string>Takasugi, Kiuchi & Opelz 1977</json:string><json:string>Tew et al. (1981)</json:string><json:string>Baker et al 1976</json:string><json:string>Tew et al. 1981</json:string><json:string>Engel et al. 1977</json:string><json:string>Budtz-Jorgensen et al. 1977</json:string><json:string>Francheschi et al. 1981</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/WNG-VRPC13VV-S</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - dentistry, oral surgery & medicine</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - dentistry</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Dentistry</json:string><json:string>3 - Periodontics</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>1984</publicationDate><copyrightDate>1984</copyrightDate><doi><json:string>10.1111/j.1600-0765.1984.tb01008.x</json:string></doi><id>76FE5864E492C71C28772616A5F2E9FCCB2BA0FC</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/76FE5864E492C71C28772616A5F2E9FCCB2BA0FC/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/76FE5864E492C71C28772616A5F2E9FCCB2BA0FC/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/76FE5864E492C71C28772616A5F2E9FCCB2BA0FC/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main">Blastogenic responsiveness of human lymphoid cells to mitogens and to homogenates of periodontal pocket bacteria</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1984-07"></date></publicationStmt><notesStmt><note type="content-type" subtype="article" source="article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="article"><analytic><title level="a" type="main">Blastogenic responsiveness of human lymphoid cells to mitogens and to homogenates of periodontal pocket bacteria</title><author xml:id="author-0000" role="corresp"><persName><forename type="first">Jon B.</forename><surname>Suzuki</surname></persName><affiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</affiliation><affiliation>Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</affiliation></author><author xml:id="author-0001" role="corresp"><persName><forename type="first">Tom J.</forename><surname>Sims</surname></persName><affiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</affiliation><affiliation>Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</affiliation></author><author xml:id="author-0002" role="corresp"><persName><forename type="first">David L.</forename><surname>Singer</surname></persName><affiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</affiliation><affiliation>Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</affiliation></author><author xml:id="author-0003" role="corresp"><persName><forename type="first">Roy C.</forename><surname>Page</surname></persName><affiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</affiliation><affiliation>Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</affiliation></author><idno type="istex">76FE5864E492C71C28772616A5F2E9FCCB2BA0FC</idno><idno type="ark">ark:/67375/WNG-VRPC13VV-S</idno><idno type="DOI">10.1111/j.1600-0765.1984.tb01008.x</idno><idno type="unit">JRE352</idno><idno type="toTypesetVersion">file:JRE.JRE352.pdf</idno></analytic><monogr><title level="j" type="main">Journal of Periodontal Research</title><title level="j" type="alt">JOURNAL OF PERIODONTAL RESEARCH</title><idno type="pISSN">0022-3484</idno><idno type="eISSN">1600-0765</idno><idno type="book-DOI">10.1111/(ISSN)1600-0765</idno><idno type="book-part-DOI">10.1111/jre.1984.19.issue-4</idno><idno type="product">JRE</idno><idno type="publisherDivision">ST</idno><imprint><biblScope unit="vol">19</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="352">352</biblScope><biblScope unit="page" to="365">365</biblScope><biblScope unit="page-count">14</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1984-07"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en" style="main"><p>Experiments aimed at demonstrating significant differences in the blastogenic responsiveness of peripheral blood mononuclear cells (PBM) activated using bacterial preparations and pokeweed mitogen (PWM) were performed. The known variables in the in vitro assay system were controlled to the greatest extent possible. A range of cell and activator concentrations, incubation times extending from 3 to 7 days, conical microtest wells, and a labeling procedure which accurately reflects blastogenesis was used. Under these test conditions, cultures of activated patient cells did not differ significantly from activated control cells in counts incorporated, in Stimulation Index, or in incubation time, cell concentration, or activator dose required for maximal blastogenesis, except for cultures activated with LPS and PWM. Responsiveness of cultures of patient cells activated with LPS was significantly lower than that of cultures of control cells (P < 0.01). Cultures from most normal control donors exposed to PWM became maximally activated at 3 days' incubation, while cultures from most patients required 5 to 7 days. Cultures of patient cells did differ significantly (p < 0.05) from control cells in that their spontaneous lymphocyte proliferation was suppressed. These observations indicate that patients with periodontitis may have abnormalities in basic immunologic control mechanisms.</p></abstract><langUsage><language ident="en"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/76FE5864E492C71C28772616A5F2E9FCCB2BA0FC/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1600-0765</doi><issn type="print">0022-3484</issn><issn type="electronic">1600-0765</issn><idGroup><id type="product" value="JRE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF PERIODONTAL RESEARCH">Journal of Periodontal Research</title></titleGroup></publicationMeta><publicationMeta level="part" position="07004"><doi origin="wiley">10.1111/jre.1984.19.issue-4</doi><numberingGroup><numbering type="journalVolume" number="19">19</numbering><numbering type="journalIssue" number="4">4</numbering></numberingGroup><coverDate startDate="1984-07">July 1984</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="0035200" status="forIssue"><doi origin="wiley">10.1111/j.1600-0765.1984.tb01008.x</doi><idGroup><id type="unit" value="JRE352"></id></idGroup><countGroup><count type="pageTotal" number="14"></count></countGroup><eventGroup><event type="firstOnline" date="2006-06-30"></event><event type="publishedOnlineFinalForm" date="2006-06-30"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-10"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-01"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="352">352</numbering><numbering type="pageLast" number="365">365</numbering></numberingGroup><correspondenceTo>Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JRE.JRE352.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Accepted for publication February 8, 1984</unparsedEditorialHistory><countGroup><count type="referenceTotal" number="50"></count><count type="linksCrossRef" number="8"></count></countGroup><titleGroup><title type="main">Blastogenic responsiveness of human lymphoid cells to mitogens and to homogenates of periodontal pocket bacteria</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" corresponding="yes"><personName><givenNames>Jon B.</givenNames><familyName>Suzuki</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1" corresponding="yes"><personName><givenNames>Tom J.</givenNames><familyName>Sims</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1" corresponding="yes"><personName><givenNames>David L.</givenNames><familyName>Singer</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1" corresponding="yes"><personName><givenNames>Roy C.</givenNames><familyName>Page</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1"><unparsedAffiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><p>Experiments aimed at demonstrating significant differences in the blastogenic responsiveness of peripheral blood mononuclear cells (PBM) activated using bacterial preparations and pokeweed mitogen (PWM) were performed. The known variables in the in vitro assay system were controlled to the greatest extent possible. A range of cell and activator concentrations, incubation times extending from 3 to 7 days, conical microtest wells, and a labeling procedure which accurately reflects blastogenesis was used. Under these test conditions, cultures of activated patient cells did not differ significantly from activated control cells in counts incorporated, in Stimulation Index, or in incubation time, cell concentration, or activator dose required for maximal blastogenesis, except for cultures activated with LPS and PWM. Responsiveness of cultures of patient cells activated with LPS was significantly lower than that of cultures of control cells (P < 0.01). Cultures from most normal control donors exposed to PWM became maximally activated at 3 days' incubation, while cultures from most patients required 5 to 7 days. Cultures of patient cells did differ significantly (p < 0.05) from control cells in that their spontaneous lymphocyte proliferation was suppressed. These observations indicate that patients with periodontitis may have abnormalities in basic immunologic control mechanisms.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Blastogenic responsiveness of human lymphoid cells to mitogens and to homogenates of periodontal pocket bacteria</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Blastogenic responsiveness of human lymphoid cells to mitogens and to homogenates of periodontal pocket bacteria</title></titleInfo><name type="personal"><namePart type="given">Jon B.</namePart><namePart type="family">Suzuki</namePart><affiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</affiliation><affiliation>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tom J.</namePart><namePart type="family">Sims</namePart><affiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</affiliation><affiliation>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">David L.</namePart><namePart type="family">Singer</namePart><affiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</affiliation><affiliation>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Roy C.</namePart><namePart type="family">Page</namePart><affiliation>Departments of Periodontics and Pathology and the Center for Research in Oral Biology, University of Washington, Seattle, Washington and the Departments of Periodontics and Microbiology, Baltimore College of Dental Surgery, University of Maryland and The Johns Hopkins Hospital, Baltimote, Maryland, U.S.A.</affiliation><affiliation>Correspondence address: Address: Department of Periodontics, Baltimore College of Dental Surgery, University of Maryland, Baltimore, Maryland 21201, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1984-07</dateIssued><edition>Accepted for publication February 8, 1984</edition><copyrightDate encoding="w3cdtf">1984</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="references">50</extent><extent unit="linksCrossRef">8</extent></physicalDescription><abstract lang="en">Experiments aimed at demonstrating significant differences in the blastogenic responsiveness of peripheral blood mononuclear cells (PBM) activated using bacterial preparations and pokeweed mitogen (PWM) were performed. The known variables in the in vitro assay system were controlled to the greatest extent possible. A range of cell and activator concentrations, incubation times extending from 3 to 7 days, conical microtest wells, and a labeling procedure which accurately reflects blastogenesis was used. Under these test conditions, cultures of activated patient cells did not differ significantly from activated control cells in counts incorporated, in Stimulation Index, or in incubation time, cell concentration, or activator dose required for maximal blastogenesis, except for cultures activated with LPS and PWM. Responsiveness of cultures of patient cells activated with LPS was significantly lower than that of cultures of control cells (P < 0.01). Cultures from most normal control donors exposed to PWM became maximally activated at 3 days' incubation, while cultures from most patients required 5 to 7 days. Cultures of patient cells did differ significantly (p < 0.05) from control cells in that their spontaneous lymphocyte proliferation was suppressed. These observations indicate that patients with periodontitis may have abnormalities in basic immunologic control mechanisms.</abstract><relatedItem type="host"><titleInfo><title>Journal of Periodontal Research</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">0022-3484</identifier><identifier type="eISSN">1600-0765</identifier><identifier type="DOI">10.1111/(ISSN)1600-0765</identifier><identifier type="PublisherID">JRE</identifier><part><date>1984</date><detail type="volume"><caption>vol.</caption><number>19</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>352</start><end>365</end><total>14</total></extent></part></relatedItem><identifier type="istex">76FE5864E492C71C28772616A5F2E9FCCB2BA0FC</identifier><identifier type="ark">ark:/67375/WNG-VRPC13VV-S</identifier><identifier type="DOI">10.1111/j.1600-0765.1984.tb01008.x</identifier><identifier type="ArticleID">JRE352</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/76FE5864E492C71C28772616A5F2E9FCCB2BA0FC/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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