Colonization of osseointegrated titanium implants in edentulous patients. Early results
Identifieur interne : 003864 ( Istex/Corpus ); précédent : 003863; suivant : 003865Colonization of osseointegrated titanium implants in edentulous patients. Early results
Auteurs : A. Mombelli ; D. Buser ; N. P. LangSource :
- Oral Microbiology and Immunology [ 0902-0055 ] ; 1988-09.
English descriptors
- KwdEn :
- Actinomyces odontolytieus, Anaerobic, Anaerobic rods, Appl microbiol, Bacteroides, Bacteroides gingivalis, Bacteroides melaninogenicus, Biochemical tests, Biol buccale, Clin microbiol, Clin periodontol, Clinical signs, Clinical success, Cocci, Coccoid cells, Colonization, Cylinder implants, Darkfield microscope, Darkfield microscopy, Dental medicine, Dental plaque, Denture, Early results, Edentulous patients, Etsa, Etsa plates, Experimental gingivitis, Facultative, Facultative cocci, First time, Fusiform organisms, Hollow cylinder implants, Implant, Implantation, Initial healing phase, International team, Longitudinal, Longitudinal development, Median, Median range, Microbiol, Microbiological, Microbiological data, Microbiota, Mombelli, Motile rods, Oral implantology, Oral microbiol immunol, Oral mucosa, Osseointegrated, Osseointegrated implants, Osseointegrated titanium implants, Particular site, Periodontal, Periodontal disease, Plaque, Plaque index, Positive samples, Predominant cultivable microflora, Preoperative swabs, Representative colonies, Significant shifts, Small amounts, Small spirochetes, Spirochete, Standard deviations, Sterile cotton pellets, Sterile paperpoints, Syed, Titanium, Total counts, Transmucosal implants.
- Teeft :
- Actinomyces odontolytieus, Anaerobic, Anaerobic rods, Appl microbiol, Bacteroides, Bacteroides gingivalis, Bacteroides melaninogenicus, Biochemical tests, Biol buccale, Clin microbiol, Clin periodontol, Clinical signs, Clinical success, Cocci, Coccoid cells, Colonization, Cylinder implants, Darkfield microscope, Darkfield microscopy, Dental medicine, Dental plaque, Denture, Early results, Edentulous patients, Etsa, Etsa plates, Experimental gingivitis, Facultative, Facultative cocci, First time, Fusiform organisms, Hollow cylinder implants, Implant, Implantation, Initial healing phase, International team, Longitudinal, Longitudinal development, Median, Median range, Microbiol, Microbiological, Microbiological data, Microbiota, Mombelli, Motile rods, Oral implantology, Oral microbiol immunol, Oral mucosa, Osseointegrated, Osseointegrated implants, Osseointegrated titanium implants, Particular site, Periodontal, Periodontal disease, Plaque, Plaque index, Positive samples, Predominant cultivable microflora, Preoperative swabs, Representative colonies, Significant shifts, Small amounts, Small spirochetes, Spirochete, Standard deviations, Sterile cotton pellets, Sterile paperpoints, Syed, Titanium, Total counts, Transmucosal implants.
Abstract
The purpose of this investigation was to study the colonization of newly set implants in edentates and to monitor the development of the predominant subgingival microbiota during the first 6 months of wearing the implants in vivo. Microbial samples of the receiving sites were taken one day prior to the insertion with sterile cotton pellets. After implantation, one site per implant was sampled with sterile paperpoints once every week for 2 months, and thereafter in monthly intervals up to 180 days. A total of 114 samples of 9 sites from 5 patients were evaluated using the darkfield microscope and cultured anaerobically on non‐selective and selective media. Representative colonies were identified by atmospheric growth capabilities, Gram‐staining characteristics and biochemical tests. Small amounts of bacteria were collected from the preoperative swabs. On an average 86% of the microorganisms were identified morphologically as coccoid cells and over 80% of the cultivated bacteria were Gram‐positive facultative cocci. After implantation, no significant changes in these proportions could be observed in all but one site. In this particular site a steady decrease of coccoid cells and a simultaneous increase of rods was observed after 21 days. Actinomyces odontolyticus was first detected at Day 21 and Fusobacterium spp. were first detected at Day 42; at Day 120 small spirochetes were found for the first time, pus formation was noted clinically and a pocket probing depth of 6 mm was recorded. None of the other sites showed probing depths of over 3 mm or pus formation. In these clinically successful sites spirochetes were never seen. Fusobacteria could only be detected in 13 of 104 samples. Black‐pigmenting Bacteroides were found infrequently and no trend of increase was apparent in any site over the 180 days of monitoring.
Url:
DOI: 10.1111/j.1399-302X.1988.tb00095.x
Links to Exploration step
ISTEX:7287DB9E195848A80085A5F1E591029C98E5498FLe document en format XML
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Mombelli</name><affiliation><mods:affiliation>University of Berne, School of Dental Medicine, Switzerland</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Dr. A. Mombelli, School of Dental Medicine, University of Berne, Freiburgstrasse 7, CH‐3010 Berne, Switzerland.</mods:affiliation></affiliation></author><author><name sortKey="Buser, D" sort="Buser, D" uniqKey="Buser D" first="D." last="Buser">D. Buser</name><affiliation><mods:affiliation>University of Berne, School of Dental Medicine, Switzerland</mods:affiliation></affiliation></author><author><name sortKey="Lang, N P" sort="Lang, N P" uniqKey="Lang N" first="N. P." last="Lang">N. P. Lang</name><affiliation><mods:affiliation>University of Berne, School of Dental Medicine, Switzerland</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Oral Microbiology and Immunology</title><title level="j" type="alt">ORAL MICROBIOLOGY IMMUNOLOGY</title><idno type="ISSN">0902-0055</idno><idno type="eISSN">1399-302X</idno><imprint><biblScope unit="vol">3</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="113">113</biblScope><biblScope unit="page" to="120">120</biblScope><biblScope unit="page-count">8</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1988-09">1988-09</date></imprint><idno type="ISSN">0902-0055</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0902-0055</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actinomyces odontolytieus</term><term>Anaerobic</term><term>Anaerobic rods</term><term>Appl microbiol</term><term>Bacteroides</term><term>Bacteroides gingivalis</term><term>Bacteroides melaninogenicus</term><term>Biochemical tests</term><term>Biol buccale</term><term>Clin microbiol</term><term>Clin periodontol</term><term>Clinical signs</term><term>Clinical success</term><term>Cocci</term><term>Coccoid cells</term><term>Colonization</term><term>Cylinder implants</term><term>Darkfield microscope</term><term>Darkfield microscopy</term><term>Dental medicine</term><term>Dental plaque</term><term>Denture</term><term>Early results</term><term>Edentulous patients</term><term>Etsa</term><term>Etsa plates</term><term>Experimental gingivitis</term><term>Facultative</term><term>Facultative cocci</term><term>First time</term><term>Fusiform organisms</term><term>Hollow cylinder implants</term><term>Implant</term><term>Implantation</term><term>Initial healing phase</term><term>International team</term><term>Longitudinal</term><term>Longitudinal development</term><term>Median</term><term>Median range</term><term>Microbiol</term><term>Microbiological</term><term>Microbiological data</term><term>Microbiota</term><term>Mombelli</term><term>Motile rods</term><term>Oral implantology</term><term>Oral microbiol immunol</term><term>Oral mucosa</term><term>Osseointegrated</term><term>Osseointegrated implants</term><term>Osseointegrated titanium implants</term><term>Particular site</term><term>Periodontal</term><term>Periodontal disease</term><term>Plaque</term><term>Plaque index</term><term>Positive samples</term><term>Predominant cultivable microflora</term><term>Preoperative swabs</term><term>Representative colonies</term><term>Significant shifts</term><term>Small amounts</term><term>Small spirochetes</term><term>Spirochete</term><term>Standard deviations</term><term>Sterile cotton pellets</term><term>Sterile paperpoints</term><term>Syed</term><term>Titanium</term><term>Total counts</term><term>Transmucosal implants</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Actinomyces odontolytieus</term><term>Anaerobic</term><term>Anaerobic rods</term><term>Appl microbiol</term><term>Bacteroides</term><term>Bacteroides gingivalis</term><term>Bacteroides melaninogenicus</term><term>Biochemical tests</term><term>Biol buccale</term><term>Clin microbiol</term><term>Clin periodontol</term><term>Clinical signs</term><term>Clinical success</term><term>Cocci</term><term>Coccoid cells</term><term>Colonization</term><term>Cylinder implants</term><term>Darkfield microscope</term><term>Darkfield microscopy</term><term>Dental medicine</term><term>Dental plaque</term><term>Denture</term><term>Early results</term><term>Edentulous patients</term><term>Etsa</term><term>Etsa plates</term><term>Experimental gingivitis</term><term>Facultative</term><term>Facultative cocci</term><term>First time</term><term>Fusiform organisms</term><term>Hollow cylinder implants</term><term>Implant</term><term>Implantation</term><term>Initial healing phase</term><term>International team</term><term>Longitudinal</term><term>Longitudinal development</term><term>Median</term><term>Median range</term><term>Microbiol</term><term>Microbiological</term><term>Microbiological data</term><term>Microbiota</term><term>Mombelli</term><term>Motile rods</term><term>Oral implantology</term><term>Oral microbiol immunol</term><term>Oral mucosa</term><term>Osseointegrated</term><term>Osseointegrated implants</term><term>Osseointegrated titanium implants</term><term>Particular site</term><term>Periodontal</term><term>Periodontal disease</term><term>Plaque</term><term>Plaque index</term><term>Positive samples</term><term>Predominant cultivable microflora</term><term>Preoperative swabs</term><term>Representative colonies</term><term>Significant shifts</term><term>Small amounts</term><term>Small spirochetes</term><term>Spirochete</term><term>Standard deviations</term><term>Sterile cotton pellets</term><term>Sterile paperpoints</term><term>Syed</term><term>Titanium</term><term>Total counts</term><term>Transmucosal implants</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The purpose of this investigation was to study the colonization of newly set implants in edentates and to monitor the development of the predominant subgingival microbiota during the first 6 months of wearing the implants in vivo. Microbial samples of the receiving sites were taken one day prior to the insertion with sterile cotton pellets. After implantation, one site per implant was sampled with sterile paperpoints once every week for 2 months, and thereafter in monthly intervals up to 180 days. A total of 114 samples of 9 sites from 5 patients were evaluated using the darkfield microscope and cultured anaerobically on non‐selective and selective media. Representative colonies were identified by atmospheric growth capabilities, Gram‐staining characteristics and biochemical tests. Small amounts of bacteria were collected from the preoperative swabs. On an average 86% of the microorganisms were identified morphologically as coccoid cells and over 80% of the cultivated bacteria were Gram‐positive facultative cocci. After implantation, no significant changes in these proportions could be observed in all but one site. In this particular site a steady decrease of coccoid cells and a simultaneous increase of rods was observed after 21 days. Actinomyces odontolyticus was first detected at Day 21 and Fusobacterium spp. were first detected at Day 42; at Day 120 small spirochetes were found for the first time, pus formation was noted clinically and a pocket probing depth of 6 mm was recorded. None of the other sites showed probing depths of over 3 mm or pus formation. In these clinically successful sites spirochetes were never seen. Fusobacteria could only be detected in 13 of 104 samples. Black‐pigmenting Bacteroides were found infrequently and no trend of increase was apparent in any site over the 180 days of monitoring.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>etsa</json:string><json:string>median range</json:string><json:string>median</json:string><json:string>implant</json:string><json:string>cocci</json:string><json:string>bacteroides</json:string><json:string>facultative</json:string><json:string>osseointegrated</json:string><json:string>microbiota</json:string><json:string>colonization</json:string><json:string>denture</json:string><json:string>microbiol</json:string><json:string>mombelli</json:string><json:string>syed</json:string><json:string>spirochete</json:string><json:string>microbiological</json:string><json:string>osseointegrated titanium implants</json:string><json:string>facultative cocci</json:string><json:string>periodontal</json:string><json:string>implantation</json:string><json:string>anaerobic rods</json:string><json:string>anaerobic</json:string><json:string>longitudinal development</json:string><json:string>particular site</json:string><json:string>small spirochetes</json:string><json:string>fusiform organisms</json:string><json:string>motile rods</json:string><json:string>plaque</json:string><json:string>longitudinal</json:string><json:string>coccoid cells</json:string><json:string>sterile cotton pellets</json:string><json:string>clin periodontol</json:string><json:string>sterile paperpoints</json:string><json:string>cylinder implants</json:string><json:string>periodontal disease</json:string><json:string>clinical signs</json:string><json:string>oral microbiol immunol</json:string><json:string>representative colonies</json:string><json:string>dental plaque</json:string><json:string>experimental gingivitis</json:string><json:string>preoperative swabs</json:string><json:string>titanium</json:string><json:string>significant shifts</json:string><json:string>plaque index</json:string><json:string>oral implantology</json:string><json:string>darkfield microscopy</json:string><json:string>edentulous patients</json:string><json:string>international team</json:string><json:string>microbiological data</json:string><json:string>hollow cylinder implants</json:string><json:string>standard deviations</json:string><json:string>clinical success</json:string><json:string>early results</json:string><json:string>initial healing phase</json:string><json:string>first time</json:string><json:string>etsa plates</json:string><json:string>transmucosal implants</json:string><json:string>total counts</json:string><json:string>bacteroides melaninogenicus</json:string><json:string>bacteroides gingivalis</json:string><json:string>positive samples</json:string><json:string>oral mucosa</json:string><json:string>osseointegrated implants</json:string><json:string>actinomyces odontolytieus</json:string><json:string>appl microbiol</json:string><json:string>dental medicine</json:string><json:string>biol buccale</json:string><json:string>clin microbiol</json:string><json:string>small amounts</json:string><json:string>biochemical tests</json:string><json:string>darkfield microscope</json:string><json:string>predominant cultivable microflora</json:string></teeft></keywords><author><json:item><name>A. Mombelli</name><affiliations><json:string>University of Berne, School of Dental Medicine, Switzerland</json:string><json:string>Correspondence address: Dr. A. Mombelli, School of Dental Medicine, University of Berne, Freiburgstrasse 7, CH‐3010 Berne, Switzerland.</json:string></affiliations></json:item><json:item><name>D. Buser</name><affiliations><json:string>University of Berne, School of Dental Medicine, Switzerland</json:string></affiliations></json:item><json:item><name>N. P. Lang</name><affiliations><json:string>University of Berne, School of Dental Medicine, Switzerland</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>titanium‐implants</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>osseointegration</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>colonization</value></json:item></subject><articleId><json:string>OMI113</json:string></articleId><arkIstex>ark:/67375/WNG-XHP2TSRX-W</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>The purpose of this investigation was to study the colonization of newly set implants in edentates and to monitor the development of the predominant subgingival microbiota during the first 6 months of wearing the implants in vivo. Microbial samples of the receiving sites were taken one day prior to the insertion with sterile cotton pellets. After implantation, one site per implant was sampled with sterile paperpoints once every week for 2 months, and thereafter in monthly intervals up to 180 days. A total of 114 samples of 9 sites from 5 patients were evaluated using the darkfield microscope and cultured anaerobically on non‐selective and selective media. Representative colonies were identified by atmospheric growth capabilities, Gram‐staining characteristics and biochemical tests. Small amounts of bacteria were collected from the preoperative swabs. On an average 86% of the microorganisms were identified morphologically as coccoid cells and over 80% of the cultivated bacteria were Gram‐positive facultative cocci. After implantation, no significant changes in these proportions could be observed in all but one site. In this particular site a steady decrease of coccoid cells and a simultaneous increase of rods was observed after 21 days. Actinomyces odontolyticus was first detected at Day 21 and Fusobacterium spp. were first detected at Day 42; at Day 120 small spirochetes were found for the first time, pus formation was noted clinically and a pocket probing depth of 6 mm was recorded. None of the other sites showed probing depths of over 3 mm or pus formation. In these clinically successful sites spirochetes were never seen. Fusobacteria could only be detected in 13 of 104 samples. Black‐pigmenting Bacteroides were found infrequently and no trend of increase was apparent in any site over the 180 days of monitoring.</abstract><qualityIndicators><score>9</score><pdfWordCount>4000</pdfWordCount><pdfCharCount>27772</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>766 x 1101 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>288</abstractWordCount><abstractCharCount>1845</abstractCharCount><keywordCount>3</keywordCount></qualityIndicators><title>Colonization of osseointegrated titanium implants in edentulous patients. Early results</title><pmid><json:string>3269993</json:string></pmid><genre><json:string>article</json:string></genre><host><title>Oral Microbiology and Immunology</title><language><json:string>unknown</json:string></language><doi><json:string>10.1111/(ISSN)1399-302X</json:string></doi><issn><json:string>0902-0055</json:string></issn><eissn><json:string>1399-302X</json:string></eissn><publisherId><json:string>OMI</json:string></publisherId><volume>3</volume><issue>3</issue><pages><first>113</first><last>120</last><total>8</total></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1988</json:string><json:string>1250</json:string></date><geogName></geogName><orgName><json:string>Straumann Institute, Waldenburg, Switzerland</json:string><json:string>University of Berne</json:string><json:string>Spiral System Inc.</json:string><json:string>Clinical Research Foundation for the Promotion of Oral Health</json:string><json:string>University of Berne, Switzerland</json:string><json:string>Switzerland, Accepted</json:string><json:string>CRF</json:string><json:string>Switzerland Mombelli A, Buser</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Rosmarie Veragut</json:string><json:string>D. Buser</json:string><json:string>Barbara Frutig</json:string></persName><placeName><json:string>Switzerland</json:string><json:string>MD</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Mombelli et al.</json:string><json:string>Gusberti et al.</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/WNG-XHP2TSRX-W</json:string></ark><categories><wos></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - dentistry</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Microbiology (medical)</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Dentistry</json:string><json:string>3 - General Dentistry</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Immunology</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Microbiology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string><json:string>4 - dermatologie</json:string></inist></categories><publicationDate>1988</publicationDate><copyrightDate>1988</copyrightDate><doi><json:string>10.1111/j.1399-302X.1988.tb00095.x</json:string></doi><id>7287DB9E195848A80085A5F1E591029C98E5498F</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/7287DB9E195848A80085A5F1E591029C98E5498F/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/7287DB9E195848A80085A5F1E591029C98E5498F/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/7287DB9E195848A80085A5F1E591029C98E5498F/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main">Colonization of osseointegrated titanium implants in edentulous patients. Early results</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1988-09"></date></publicationStmt><notesStmt><note type="content-type" subtype="article" source="article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="article"><analytic><title level="a" type="main">Colonization of osseointegrated titanium implants in edentulous patients. Early results</title><author xml:id="author-0000" role="corresp"><persName><forename type="first">A.</forename><surname>Mombelli</surname></persName><affiliation>University of Berne, School of Dental Medicine, Switzerland<address><country key="CH"></country></address></affiliation><affiliation>Dr. A. Mombelli, School of Dental Medicine, University of Berne, Freiburgstrasse 7, CH‐3010 Berne, Switzerland.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">D.</forename><surname>Buser</surname></persName><affiliation>University of Berne, School of Dental Medicine, Switzerland<address><country key="CH"></country></address></affiliation></author><author xml:id="author-0002"><persName><forename type="first">N. P.</forename><surname>Lang</surname></persName><affiliation>University of Berne, School of Dental Medicine, Switzerland<address><country key="CH"></country></address></affiliation></author><idno type="istex">7287DB9E195848A80085A5F1E591029C98E5498F</idno><idno type="ark">ark:/67375/WNG-XHP2TSRX-W</idno><idno type="DOI">10.1111/j.1399-302X.1988.tb00095.x</idno><idno type="unit">OMI113</idno><idno type="toTypesetVersion">file:OMI.OMI113.pdf</idno></analytic><monogr><title level="j" type="main">Oral Microbiology and Immunology</title><title level="j" type="alt">ORAL MICROBIOLOGY IMMUNOLOGY</title><idno type="pISSN">0902-0055</idno><idno type="eISSN">1399-302X</idno><idno type="book-DOI">10.1111/(ISSN)1399-302X</idno><idno type="book-part-DOI">10.1111/omi.1988.3.issue-3</idno><idno type="product">OMI</idno><idno type="publisherDivision">ST</idno><imprint><biblScope unit="vol">3</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="113">113</biblScope><biblScope unit="page" to="120">120</biblScope><biblScope unit="page-count">8</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1988-09"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><abstract xml:lang="en" style="main"><p>The purpose of this investigation was to study the colonization of newly set implants in edentates and to monitor the development of the predominant subgingival microbiota during the first 6 months of wearing the implants <hi rend="italic">in vivo.</hi> Microbial samples of the receiving sites were taken one day prior to the insertion with sterile cotton pellets. After implantation, one site per implant was sampled with sterile paperpoints once every week for 2 months, and thereafter in monthly intervals up to 180 days. A total of 114 samples of 9 sites from 5 patients were evaluated using the darkfield microscope and cultured anaerobically on non‐selective and selective media. Representative colonies were identified by atmospheric growth capabilities, Gram‐staining characteristics and biochemical tests. Small amounts of bacteria were collected from the preoperative swabs. On an average 86% of the microorganisms were identified morphologically as coccoid cells and over 80% of the cultivated bacteria were Gram‐positive facultative cocci. After implantation, no significant changes in these proportions could be observed in all but one site. In this particular site a steady decrease of coccoid cells and a simultaneous increase of rods was observed after 21 days. <hi rend="italic">Actinomyces odontolyticus</hi> was first detected at Day 21 and <hi rend="italic">Fusobacterium</hi> spp. were first detected at Day 42; at Day 120 small spirochetes were found for the first time, pus formation was noted clinically and a pocket probing depth of 6 mm was recorded. None of the other sites showed probing depths of over 3 mm or pus formation. In these clinically successful sites spirochetes were never seen. Fusobacteria could only be detected in 13 of 104 samples. Black‐pigmenting <hi rend="italic">Bacteroides</hi> were found infrequently and no trend of increase was apparent in any site over the 180 days of monitoring.</p></abstract><textClass><keywords xml:lang="en"><term xml:id="k1">titanium‐implants</term><term xml:id="k2">osseointegration</term><term xml:id="k3">colonization</term></keywords><keywords rend="tocHeading1"><term>Original Article</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/7287DB9E195848A80085A5F1E591029C98E5498F/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1399-302X</doi><issn type="print">0902-0055</issn><issn type="electronic">1399-302X</issn><idGroup><id type="product" value="OMI"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="ORAL MICROBIOLOGY IMMUNOLOGY">Oral Microbiology and Immunology</title></titleGroup></publicationMeta><publicationMeta level="part" position="09003"><doi origin="wiley">10.1111/omi.1988.3.issue-3</doi><numberingGroup><numbering type="journalVolume" number="3">3</numbering><numbering type="journalIssue" number="3">3</numbering></numberingGroup><coverDate startDate="1988-09">September 1988</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="0011300" status="forIssue"><doi origin="wiley">10.1111/j.1399-302X.1988.tb00095.x</doi><idGroup><id type="unit" value="OMI113"></id></idGroup><countGroup><count type="pageTotal" number="8"></count></countGroup><titleGroup><title type="tocHeading1">Original Article</title></titleGroup><eventGroup><event type="firstOnline" date="2007-12-18"></event><event type="publishedOnlineFinalForm" date="2007-12-18"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-09"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-06"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-11-03"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="113">113</numbering><numbering type="pageLast" number="120">120</numbering></numberingGroup><correspondenceTo>Dr. A. Mombelli, School of Dental Medicine, University of Berne, Freiburgstrasse 7, CH‐3010 Berne, Switzerland.</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:OMI.OMI113.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Accepted for publication April 30, 1988</unparsedEditorialHistory><countGroup><count type="referenceTotal" number="36"></count><count type="linksCrossRef" number="4"></count></countGroup><titleGroup><title type="main">Colonization of osseointegrated titanium implants in edentulous patients. Early results</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" corresponding="yes"><personName><givenNames>A.</givenNames><familyName>Mombelli</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>D.</givenNames><familyName>Buser</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>N. P.</givenNames><familyName>Lang</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CH"><unparsedAffiliation>University of Berne, School of Dental Medicine, Switzerland</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">titanium‐implants</keyword><keyword xml:id="k2">osseointegration</keyword><keyword xml:id="k3">colonization</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p>The purpose of this investigation was to study the colonization of newly set implants in edentates and to monitor the development of the predominant subgingival microbiota during the first 6 months of wearing the implants <i>in vivo.</i> Microbial samples of the receiving sites were taken one day prior to the insertion with sterile cotton pellets. After implantation, one site per implant was sampled with sterile paperpoints once every week for 2 months, and thereafter in monthly intervals up to 180 days. A total of 114 samples of 9 sites from 5 patients were evaluated using the darkfield microscope and cultured anaerobically on non‐selective and selective media. Representative colonies were identified by atmospheric growth capabilities, Gram‐staining characteristics and biochemical tests. Small amounts of bacteria were collected from the preoperative swabs. On an average 86% of the microorganisms were identified morphologically as coccoid cells and over 80% of the cultivated bacteria were Gram‐positive facultative cocci. After implantation, no significant changes in these proportions could be observed in all but one site. In this particular site a steady decrease of coccoid cells and a simultaneous increase of rods was observed after 21 days. <i>Actinomyces odontolyticus</i> was first detected at Day 21 and <i>Fusobacterium</i> spp. were first detected at Day 42; at Day 120 small spirochetes were found for the first time, pus formation was noted clinically and a pocket probing depth of 6 mm was recorded. None of the other sites showed probing depths of over 3 mm or pus formation. In these clinically successful sites spirochetes were never seen. Fusobacteria could only be detected in 13 of 104 samples. Black‐pigmenting <i>Bacteroides</i> were found infrequently and no trend of increase was apparent in any site over the 180 days of monitoring.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Colonization of osseointegrated titanium implants in edentulous patients. Early results</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Colonization of osseointegrated titanium implants in edentulous patients. Early results</title></titleInfo><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Mombelli</namePart><affiliation>University of Berne, School of Dental Medicine, Switzerland</affiliation><affiliation>Correspondence address: Dr. A. Mombelli, School of Dental Medicine, University of Berne, Freiburgstrasse 7, CH‐3010 Berne, Switzerland.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Buser</namePart><affiliation>University of Berne, School of Dental Medicine, Switzerland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N. P.</namePart><namePart type="family">Lang</namePart><affiliation>University of Berne, School of Dental Medicine, Switzerland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1988-09</dateIssued><edition>Accepted for publication April 30, 1988</edition><copyrightDate encoding="w3cdtf">1988</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="references">36</extent><extent unit="linksCrossRef">4</extent></physicalDescription><abstract lang="en">The purpose of this investigation was to study the colonization of newly set implants in edentates and to monitor the development of the predominant subgingival microbiota during the first 6 months of wearing the implants in vivo. Microbial samples of the receiving sites were taken one day prior to the insertion with sterile cotton pellets. After implantation, one site per implant was sampled with sterile paperpoints once every week for 2 months, and thereafter in monthly intervals up to 180 days. A total of 114 samples of 9 sites from 5 patients were evaluated using the darkfield microscope and cultured anaerobically on non‐selective and selective media. Representative colonies were identified by atmospheric growth capabilities, Gram‐staining characteristics and biochemical tests. Small amounts of bacteria were collected from the preoperative swabs. On an average 86% of the microorganisms were identified morphologically as coccoid cells and over 80% of the cultivated bacteria were Gram‐positive facultative cocci. After implantation, no significant changes in these proportions could be observed in all but one site. In this particular site a steady decrease of coccoid cells and a simultaneous increase of rods was observed after 21 days. Actinomyces odontolyticus was first detected at Day 21 and Fusobacterium spp. were first detected at Day 42; at Day 120 small spirochetes were found for the first time, pus formation was noted clinically and a pocket probing depth of 6 mm was recorded. None of the other sites showed probing depths of over 3 mm or pus formation. In these clinically successful sites spirochetes were never seen. Fusobacteria could only be detected in 13 of 104 samples. Black‐pigmenting Bacteroides were found infrequently and no trend of increase was apparent in any site over the 180 days of monitoring.</abstract><subject lang="en"><genre>keywords</genre><topic>titanium‐implants</topic><topic>osseointegration</topic><topic>colonization</topic></subject><relatedItem type="host"><titleInfo><title>Oral Microbiology and Immunology</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">0902-0055</identifier><identifier type="eISSN">1399-302X</identifier><identifier type="DOI">10.1111/(ISSN)1399-302X</identifier><identifier type="PublisherID">OMI</identifier><part><date>1988</date><detail type="volume"><caption>vol.</caption><number>3</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>113</start><end>120</end><total>8</total></extent></part></relatedItem><identifier type="istex">7287DB9E195848A80085A5F1E591029C98E5498F</identifier><identifier type="ark">ark:/67375/WNG-XHP2TSRX-W</identifier><identifier type="DOI">10.1111/j.1399-302X.1988.tb00095.x</identifier><identifier type="ArticleID">OMI113</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/7287DB9E195848A80085A5F1E591029C98E5498F/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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