Cellular Fatty Acid Analysis and Enzyme Profiles of Porphyromonas catoniae — a Frequent Colonizer of the Oral Cavity in Children
Identifieur interne : 002E10 ( Istex/Corpus ); précédent : 002E09; suivant : 002E11Cellular Fatty Acid Analysis and Enzyme Profiles of Porphyromonas catoniae — a Frequent Colonizer of the Oral Cavity in Children
Auteurs : E. Könönen ; M.-L. V Is Nen ; S. M. Finegold ; R. Heine ; H. Jousimies-SomerSource :
- Anaerobe [ 1075-9964 ] ; 1996.
English descriptors
- KwdEn :
- API ZYM, Abdominal abscess, Acid analysis, Anaerobe reference laboratory, Anaerobic, Anaerobic bacilli, Anaerobic species, Atcc, Bacterial species, Bacteroides, Broth culture, Catoniae, Clin, Clin microbiol, Cluster analysis, Crevice, Enzymatic reactions, Fatty acid, Genus porphyromonas, Gingival, Gingival crevices, Health institute, Lactamase, Lactamase production, Leucine arylamidase, Main cfas, Major amounts, Microbiol, Moderate numbers, Numerical analysis, Opportunistic pathogen, Oral cavity, Oral microbiol immunol, Oral mucosal surfaces, Oribaculum catoniae, Periodontal pockets, Porphyromonas, Porphyromonas catoniae, Positive reactions, Present study, Previous study, Prevotella melaninogenica, Prevotella species, Propionic acid, Rosco diagnostica, Similarity indices, Sodium polyanethol sulfonate, Subgroup, Syst bacteriol, Trypsin, Type strain, Variable reactions, Wide heterogeneity, Young children, cellular fatty acid analysis, children, oral flora.
- Teeft :
- Abdominal abscess, Acid analysis, Anaerobe reference laboratory, Anaerobic, Anaerobic bacilli, Anaerobic species, Atcc, Bacterial species, Bacteroides, Broth culture, Catoniae, Clin, Clin microbiol, Cluster analysis, Crevice, Enzymatic reactions, Fatty acid, Genus porphyromonas, Gingival, Gingival crevices, Health institute, Lactamase, Lactamase production, Leucine arylamidase, Main cfas, Major amounts, Microbiol, Moderate numbers, Numerical analysis, Opportunistic pathogen, Oral cavity, Oral microbiol immunol, Oral mucosal surfaces, Oribaculum catoniae, Periodontal pockets, Porphyromonas, Porphyromonas catoniae, Positive reactions, Present study, Previous study, Prevotella melaninogenica, Prevotella species, Propionic acid, Rosco diagnostica, Similarity indices, Sodium polyanethol sulfonate, Subgroup, Syst bacteriol, Trypsin, Type strain, Variable reactions, Wide heterogeneity, Young children.
Abstract
Abstract: During a previous study on the Gram-negative anaerobic microflora in children we frequently isolated a non-pigmentedPrevotella-like species with phenotypic characteristics not conforming to those of previously described species at the time. Among recently described Gram-negative anaerobic bacilli isolated from gingival crevicesPorphyromonas catoniae(BacteroidesD26) most closely resembled our isolates biochemically which also typically produced a large amount of propionic acid. Sixty-three isolates originated from oral mucosal surfaces pooled to saliva and from the gingival crevices of 23 healthy children (mean age 32 months). In addition, one biochemically similar strain which was isolated from an abdominal abscess was included in the tests. The aim of the present study was to determine the cellular fatty acid (CFA) composition for the precise identification of our isolates, and further, to determine the enzyme profiles by API ZYM to emend the description of this species. CFA profiles agreed with the identification ofBacteroidesD26 (P. catoniae). API ZYM patterns revealed positive reactions for β-galactosidase, α-glucosidase (except for one isolate), N-acetyl-β-glucosaminidase and α-fucosidase, and variable reactions for leucine arylamidase, trypsin, chymotrypsin and β-galactosidase. However, numerical analysis of the CFA data separated this species into at least three subgroups. Twenty-five oral isolates formed one subgroup which most closely resembled the type strain (ATCC 51270). The ubiquitous presence ofP. catoniaein moderate numbers in the studied oral samples suggests that this anaerobic species belongs to the normal oral flora of children even at this young age; nevertheless, it may occasionally act as an opportunistic pathogen outside the oral cavity.
Url:
DOI: 10.1006/anae.1996.0042
Links to Exploration step
ISTEX:5DF59AAF439CD927216C177F355AA70E3E6C18A2Le document en format XML
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Könönen</name><affiliation><mods:affiliation>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</mods:affiliation></affiliation></author><author><name sortKey="V Is Nen, M L" sort="V Is Nen, M L" uniqKey="V Is Nen M" first="M.-L." last="V Is Nen">M.-L. V Is Nen</name><affiliation><mods:affiliation>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</mods:affiliation></affiliation></author><author><name sortKey="Finegold, S M" sort="Finegold, S M" uniqKey="Finegold S" first="S. M." last="Finegold">S. M. Finegold</name><affiliation><mods:affiliation>Wadsworth V.A. Medical Center, West Los Angeles and UCLA School of Medicine, Los Angeles, California, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Heine, R" sort="Heine, R" uniqKey="Heine R" first="R." last="Heine">R. 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Among recently described Gram-negative anaerobic bacilli isolated from gingival crevicesPorphyromonas catoniae(BacteroidesD26) most closely resembled our isolates biochemically which also typically produced a large amount of propionic acid. Sixty-three isolates originated from oral mucosal surfaces pooled to saliva and from the gingival crevices of 23 healthy children (mean age 32 months). In addition, one biochemically similar strain which was isolated from an abdominal abscess was included in the tests. The aim of the present study was to determine the cellular fatty acid (CFA) composition for the precise identification of our isolates, and further, to determine the enzyme profiles by API ZYM to emend the description of this species. CFA profiles agreed with the identification ofBacteroidesD26 (P. catoniae). API ZYM patterns revealed positive reactions for β-galactosidase, α-glucosidase (except for one isolate), N-acetyl-β-glucosaminidase and α-fucosidase, and variable reactions for leucine arylamidase, trypsin, chymotrypsin and β-galactosidase. However, numerical analysis of the CFA data separated this species into at least three subgroups. Twenty-five oral isolates formed one subgroup which most closely resembled the type strain (ATCC 51270). The ubiquitous presence ofP. catoniaein moderate numbers in the studied oral samples suggests that this anaerobic species belongs to the normal oral flora of children even at this young age; nevertheless, it may occasionally act as an opportunistic pathogen outside the oral cavity.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>catoniae</json:string><json:string>atcc</json:string><json:string>porphyromonas</json:string><json:string>bacteroides</json:string><json:string>microbiol</json:string><json:string>gingival</json:string><json:string>oral cavity</json:string><json:string>trypsin</json:string><json:string>lactamase</json:string><json:string>clin</json:string><json:string>subgroup</json:string><json:string>abdominal abscess</json:string><json:string>anaerobic</json:string><json:string>acid analysis</json:string><json:string>oral microbiol immunol</json:string><json:string>oral mucosal surfaces</json:string><json:string>gingival crevices</json:string><json:string>lactamase production</json:string><json:string>present study</json:string><json:string>propionic acid</json:string><json:string>anaerobic species</json:string><json:string>anaerobic bacilli</json:string><json:string>porphyromonas catoniae</json:string><json:string>syst bacteriol</json:string><json:string>type strain</json:string><json:string>previous study</json:string><json:string>positive reactions</json:string><json:string>variable reactions</json:string><json:string>clin microbiol</json:string><json:string>cluster analysis</json:string><json:string>fatty acid</json:string><json:string>oribaculum catoniae</json:string><json:string>genus porphyromonas</json:string><json:string>wide heterogeneity</json:string><json:string>periodontal pockets</json:string><json:string>broth culture</json:string><json:string>sodium polyanethol sulfonate</json:string><json:string>rosco diagnostica</json:string><json:string>major amounts</json:string><json:string>enzymatic reactions</json:string><json:string>prevotella species</json:string><json:string>similarity indices</json:string><json:string>young children</json:string><json:string>bacterial species</json:string><json:string>main cfas</json:string><json:string>opportunistic pathogen</json:string><json:string>numerical analysis</json:string><json:string>prevotella melaninogenica</json:string><json:string>leucine arylamidase</json:string><json:string>anaerobe reference laboratory</json:string><json:string>health institute</json:string><json:string>moderate numbers</json:string><json:string>crevice</json:string></teeft></keywords><author><json:item><name>E. Könönen</name><affiliations><json:string>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</json:string></affiliations></json:item><json:item><name>M.-L. Väisänen</name><affiliations><json:string>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</json:string></affiliations></json:item><json:item><name>S.M. Finegold</name><affiliations><json:string>Wadsworth V.A. Medical Center, West Los Angeles and UCLA School of Medicine, Los Angeles, California, U.S.A.</json:string></affiliations></json:item><json:item><name>R. Heine</name><affiliations><json:string>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</json:string></affiliations></json:item><json:item><name>H. Jousimies-Somer</name><affiliations><json:string>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Porphyromonas catoniae</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cellular fatty acid analysis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>API ZYM</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>oral flora</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>children</value></json:item></subject><arkIstex>ark:/67375/6H6-PJSQQTG7-6</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Miscellaneous</json:string></originalGenre><abstract>Abstract: During a previous study on the Gram-negative anaerobic microflora in children we frequently isolated a non-pigmentedPrevotella-like species with phenotypic characteristics not conforming to those of previously described species at the time. Among recently described Gram-negative anaerobic bacilli isolated from gingival crevicesPorphyromonas catoniae(BacteroidesD26) most closely resembled our isolates biochemically which also typically produced a large amount of propionic acid. Sixty-three isolates originated from oral mucosal surfaces pooled to saliva and from the gingival crevices of 23 healthy children (mean age 32 months). In addition, one biochemically similar strain which was isolated from an abdominal abscess was included in the tests. The aim of the present study was to determine the cellular fatty acid (CFA) composition for the precise identification of our isolates, and further, to determine the enzyme profiles by API ZYM to emend the description of this species. CFA profiles agreed with the identification ofBacteroidesD26 (P. catoniae). API ZYM patterns revealed positive reactions for β-galactosidase, α-glucosidase (except for one isolate), N-acetyl-β-glucosaminidase and α-fucosidase, and variable reactions for leucine arylamidase, trypsin, chymotrypsin and β-galactosidase. However, numerical analysis of the CFA data separated this species into at least three subgroups. Twenty-five oral isolates formed one subgroup which most closely resembled the type strain (ATCC 51270). The ubiquitous presence ofP. catoniaein moderate numbers in the studied oral samples suggests that this anaerobic species belongs to the normal oral flora of children even at this young age; nevertheless, it may occasionally act as an opportunistic pathogen outside the oral cavity.</abstract><qualityIndicators><refBibsNative>false</refBibsNative><abstractWordCount>247</abstractWordCount><abstractCharCount>1800</abstractCharCount><keywordCount>5</keywordCount><score>8.025</score><pdfWordCount>3061</pdfWordCount><pdfCharCount>21620</pdfCharCount><pdfVersion>1.1</pdfVersion><pdfPageCount>7</pdfPageCount><pdfPageSize>595 x 842 pts (A4)</pdfPageSize></qualityIndicators><title>Cellular Fatty Acid Analysis and Enzyme Profiles of Porphyromonas catoniae — a Frequent Colonizer of the Oral Cavity in Children</title><pii><json:string>S1075-9964(96)90042-6</json:string></pii><genre><json:string>other</json:string></genre><host><title>Anaerobe</title><language><json:string>unknown</json:string></language><publicationDate>1996</publicationDate><issn><json:string>1075-9964</json:string></issn><pii><json:string>S1075-9964(00)X0035-2</json:string></pii><volume>2</volume><issue>5</issue><pages><first>329</first><last>335</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>16S</json:string><json:string>1996</json:string></date><geogName></geogName><orgName><json:string>Medical Center</json:string><json:string>Microbial ID, Inc.</json:string><json:string>Department of Periodontology, Institute of Dentistry, University of Helsinki, Helsinki, Finland</json:string><json:string>Innovative Diagnostic Systems</json:string><json:string>Laboratory, National Public Health Institute, Mannerheimintie</json:string><json:string>Hewlett-Packard Co, Palo Alto</json:string><json:string>Microbial ID, Inc., Newark</json:string><json:string>Wadsworth Anaerobe Laboratory Collection, Los Angeles, CA</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>L. Vaisanen</json:string><json:string>R. Heine</json:string><json:string>H. Jousimies-Somer</json:string><json:string>S.M. Finegold</json:string></persName><placeName><json:string>Finland</json:string><json:string>Helsinki</json:string><json:string>Los Angeles</json:string><json:string>Denmark</json:string><json:string>France</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>[12]</json:string><json:string>[11]</json:string><json:string>[17]</json:string><json:string>[1]</json:string><json:string>Moore et al. [11]</json:string><json:string>[11,18,19]</json:string><json:string>[3]</json:string><json:string>[9,10]</json:string><json:string>E. Kononen et al.</json:string><json:string>[15]</json:string><json:string>Goldstein et al. [14]</json:string><json:string>[2]</json:string><json:string>[13]</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-PJSQQTG7-6</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - microbiology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - microbiology</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Infectious Diseases</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Microbiology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1996</publicationDate><copyrightDate>1996</copyrightDate><doi><json:string>10.1006/anae.1996.0042</json:string></doi><id>5DF59AAF439CD927216C177F355AA70E3E6C18A2</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/5DF59AAF439CD927216C177F355AA70E3E6C18A2/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/5DF59AAF439CD927216C177F355AA70E3E6C18A2/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/5DF59AAF439CD927216C177F355AA70E3E6C18A2/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Cellular Fatty Acid Analysis and Enzyme Profiles of Porphyromonas catoniae — a Frequent Colonizer of the Oral Cavity in Children</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1996</date></publicationStmt><notesStmt><note type="content">Section title: Regular Paper</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Cellular Fatty Acid Analysis and Enzyme Profiles of Porphyromonas catoniae — a Frequent Colonizer of the Oral Cavity in Children</title><author xml:id="author-0000"><persName><forename type="first">E.</forename><surname>Könönen</surname></persName><affiliation>Address correspondence to: Eija Könönen, Anaerobe Reference Laboratory, National Public Health Institute, Mannerheimintie 166, FIN-00300 Helsinki, Finland</affiliation><affiliation>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</affiliation></author><author xml:id="author-0001"><persName><forename type="first">M.-L.</forename><surname>Väisänen</surname></persName><affiliation>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</affiliation></author><author xml:id="author-0002"><persName><forename type="first">S.M.</forename><surname>Finegold</surname></persName><affiliation>Wadsworth V.A. Medical Center, West Los Angeles and UCLA School of Medicine, Los Angeles, California, U.S.A.</affiliation></author><author xml:id="author-0003"><persName><forename type="first">R.</forename><surname>Heine</surname></persName><affiliation>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</affiliation></author><author xml:id="author-0004"><persName><forename type="first">H.</forename><surname>Jousimies-Somer</surname></persName><affiliation>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</affiliation></author><idno type="istex">5DF59AAF439CD927216C177F355AA70E3E6C18A2</idno><idno type="DOI">10.1006/anae.1996.0042</idno><idno type="PII">S1075-9964(96)90042-6</idno></analytic><monogr><title level="j">Anaerobe</title><title level="j" type="abbrev">YANAE</title><idno type="pISSN">1075-9964</idno><idno type="PII">S1075-9964(00)X0035-2</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996"></date><biblScope unit="volume">2</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="329">329</biblScope><biblScope unit="page" to="335">335</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1996</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>During a previous study on the Gram-negative anaerobic microflora in children we frequently isolated a non-pigmentedPrevotella-like species with phenotypic characteristics not conforming to those of previously described species at the time. Among recently described Gram-negative anaerobic bacilli isolated from gingival crevicesPorphyromonas catoniae(BacteroidesD26) most closely resembled our isolates biochemically which also typically produced a large amount of propionic acid. Sixty-three isolates originated from oral mucosal surfaces pooled to saliva and from the gingival crevices of 23 healthy children (mean age 32 months). In addition, one biochemically similar strain which was isolated from an abdominal abscess was included in the tests. The aim of the present study was to determine the cellular fatty acid (CFA) composition for the precise identification of our isolates, and further, to determine the enzyme profiles by API ZYM to emend the description of this species. CFA profiles agreed with the identification ofBacteroidesD26 (P. catoniae). API ZYM patterns revealed positive reactions for β-galactosidase, α-glucosidase (except for one isolate), N-acetyl-β-glucosaminidase and α-fucosidase, and variable reactions for leucine arylamidase, trypsin, chymotrypsin and β-galactosidase. However, numerical analysis of the CFA data separated this species into at least three subgroups. Twenty-five oral isolates formed one subgroup which most closely resembled the type strain (ATCC 51270). The ubiquitous presence ofP. catoniaein moderate numbers in the studied oral samples suggests that this anaerobic species belongs to the normal oral flora of children even at this young age; nevertheless, it may occasionally act as an opportunistic pathogen outside the oral cavity.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Porphyromonas catoniae</term></item><item><term>cellular fatty acid analysis</term></item><item><term>API ZYM</term></item><item><term>oral flora</term></item><item><term>children</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1996">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2017-09-30">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/5DF59AAF439CD927216C177F355AA70E3E6C18A2/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="mis" xml:lang="en"><item-info><jid>YANAE</jid><aid>90042</aid><ce:pii>S1075-9964(96)90042-6</ce:pii><ce:doi>10.1006/anae.1996.0042</ce:doi><ce:copyright type="other" year="1996"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Paper</ce:textfn></ce:dochead><ce:title>Cellular Fatty Acid Analysis and Enzyme Profiles of<ce:bold><ce:italic>Porphyromonas catoniae</ce:italic></ce:bold>— a Frequent Colonizer of the Oral Cavity in Children</ce:title><ce:author-group><ce:author><ce:given-name>E.</ce:given-name><ce:surname>Könönen</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="A2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="FN1"><ce:sup>f1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.-L.</ce:given-name><ce:surname>Väisänen</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>S.M.</ce:given-name><ce:surname>Finegold</ce:surname><ce:cross-ref refid="A3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>R.</ce:given-name><ce:surname>Heine</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>H.</ce:given-name><ce:surname>Jousimies-Somer</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="A1"><ce:label>a</ce:label><ce:textfn>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</ce:textfn></ce:affiliation><ce:affiliation id="A2"><ce:label>b</ce:label><ce:textfn>Department of Periodontology, Institute of Dentistry, University of Helsinki, Helsinki, Finland</ce:textfn></ce:affiliation><ce:affiliation id="A3"><ce:label>c</ce:label><ce:textfn>Wadsworth V.A. Medical Center, West Los Angeles and UCLA School of Medicine, Los Angeles, California, U.S.A.</ce:textfn></ce:affiliation><ce:footnote id="FN1"><ce:label>f1</ce:label><ce:note-para>Address correspondence to: Eija Könönen, Anaerobe Reference Laboratory, National Public Health Institute, Mannerheimintie 166, FIN-00300 Helsinki, Finland</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="8" month="3" year="1996"></ce:date-received><ce:date-accepted day="30" month="7" year="1996"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>During a previous study on the Gram-negative anaerobic microflora in children we frequently isolated a non-pigmented<ce:italic>Prevotella-</ce:italic>like species with phenotypic characteristics not conforming to those of previously described species at the time. Among recently described Gram-negative anaerobic bacilli isolated from gingival crevices<ce:italic>Porphyromonas catoniae</ce:italic>(<ce:italic>Bacteroides</ce:italic>D26) most closely resembled our isolates biochemically which also typically produced a large amount of propionic acid. Sixty-three isolates originated from oral mucosal surfaces pooled to saliva and from the gingival crevices of 23 healthy children (mean age 32 months). In addition, one biochemically similar strain which was isolated from an abdominal abscess was included in the tests. The aim of the present study was to determine the cellular fatty acid (CFA) composition for the precise identification of our isolates, and further, to determine the enzyme profiles by API ZYM to emend the description of this species. CFA profiles agreed with the identification of<ce:italic>Bacteroides</ce:italic>D26 (<ce:italic>P. catoniae</ce:italic>). API ZYM patterns revealed positive reactions for β-galactosidase, α-glucosidase (except for one isolate), N-acetyl-β-glucosaminidase and α-fucosidase, and variable reactions for leucine arylamidase, trypsin, chymotrypsin and β-galactosidase. However, numerical analysis of the CFA data separated this species into at least three subgroups. Twenty-five oral isolates formed one subgroup which most closely resembled the type strain (ATCC 51270). The ubiquitous presence of<ce:italic>P. catoniae</ce:italic>in moderate numbers in the studied oral samples suggests that this anaerobic species belongs to the normal oral flora of children even at this young age; nevertheless, it may occasionally act as an opportunistic pathogen outside the oral cavity.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text><ce:italic>Porphyromonas catoniae</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>cellular fatty acid analysis</ce:text></ce:keyword><ce:keyword><ce:text>API ZYM</ce:text></ce:keyword><ce:keyword><ce:text>oral flora</ce:text></ce:keyword><ce:keyword><ce:text>children</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Cellular Fatty Acid Analysis and Enzyme Profiles of Porphyromonas catoniae — a Frequent Colonizer of the Oral Cavity in Children</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Cellular Fatty Acid Analysis and Enzyme Profiles of</title></titleInfo><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Könönen</namePart><affiliation>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</affiliation><description>Address correspondence to: Eija Könönen, Anaerobe Reference Laboratory, National Public Health Institute, Mannerheimintie 166, FIN-00300 Helsinki, Finland</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.-L.</namePart><namePart type="family">Väisänen</namePart><affiliation>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.M.</namePart><namePart type="family">Finegold</namePart><affiliation>Wadsworth V.A. Medical Center, West Los Angeles and UCLA School of Medicine, Los Angeles, California, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Heine</namePart><affiliation>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Jousimies-Somer</namePart><affiliation>Anaerobe Reference Laboratory, National Public Health Institute, Helsinki, Finland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="other" displayLabel="Miscellaneous" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-7474895G-0">other</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1996</dateIssued><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: During a previous study on the Gram-negative anaerobic microflora in children we frequently isolated a non-pigmentedPrevotella-like species with phenotypic characteristics not conforming to those of previously described species at the time. Among recently described Gram-negative anaerobic bacilli isolated from gingival crevicesPorphyromonas catoniae(BacteroidesD26) most closely resembled our isolates biochemically which also typically produced a large amount of propionic acid. Sixty-three isolates originated from oral mucosal surfaces pooled to saliva and from the gingival crevices of 23 healthy children (mean age 32 months). In addition, one biochemically similar strain which was isolated from an abdominal abscess was included in the tests. The aim of the present study was to determine the cellular fatty acid (CFA) composition for the precise identification of our isolates, and further, to determine the enzyme profiles by API ZYM to emend the description of this species. CFA profiles agreed with the identification ofBacteroidesD26 (P. catoniae). API ZYM patterns revealed positive reactions for β-galactosidase, α-glucosidase (except for one isolate), N-acetyl-β-glucosaminidase and α-fucosidase, and variable reactions for leucine arylamidase, trypsin, chymotrypsin and β-galactosidase. However, numerical analysis of the CFA data separated this species into at least three subgroups. Twenty-five oral isolates formed one subgroup which most closely resembled the type strain (ATCC 51270). The ubiquitous presence ofP. catoniaein moderate numbers in the studied oral samples suggests that this anaerobic species belongs to the normal oral flora of children even at this young age; nevertheless, it may occasionally act as an opportunistic pathogen outside the oral cavity.</abstract><note type="content">Section title: Regular Paper</note><subject lang="en"><genre>Keywords</genre><topic>Porphyromonas catoniae</topic><topic>cellular fatty acid analysis</topic><topic>API ZYM</topic><topic>oral flora</topic><topic>children</topic></subject><relatedItem type="host"><titleInfo><title>Anaerobe</title></titleInfo><titleInfo type="abbreviated"><title>YANAE</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">199610</dateIssued></originInfo><identifier type="ISSN">1075-9964</identifier><identifier type="PII">S1075-9964(00)X0035-2</identifier><part><date>199610</date><detail type="volume"><number>2</number><caption>vol.</caption></detail><detail type="issue"><number>5</number><caption>no.</caption></detail><extent unit="issue-pages"><start>263</start><end>335</end></extent><extent unit="pages"><start>329</start><end>335</end></extent></part></relatedItem><identifier type="istex">5DF59AAF439CD927216C177F355AA70E3E6C18A2</identifier><identifier type="ark">ark:/67375/6H6-PJSQQTG7-6</identifier><identifier type="DOI">10.1006/anae.1996.0042</identifier><identifier type="PII">S1075-9964(96)90042-6</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/5DF59AAF439CD927216C177F355AA70E3E6C18A2/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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