Evaluation of chemically modified SLA implants (modSLA) biofunctionalized with integrin (RGD)‐ and heparin (KRSR)‐binding peptides
Identifieur interne : 000D83 ( Istex/Corpus ); précédent : 000D82; suivant : 000D84Evaluation of chemically modified SLA implants (modSLA) biofunctionalized with integrin (RGD)‐ and heparin (KRSR)‐binding peptides
Auteurs : Nina Broggini ; Samuele Tosatti ; Stephen J. Ferguson ; Martin Schuler ; Marcus Textor ; Michael M. Bornstein ; Dieter D. Bosshardt ; Daniel BuserSource :
- Journal of Biomedical Materials Research Part A [ 1549-3296 ] ; 2012-03.
English descriptors
- KwdEn :
- Active peptides, Additional improvement, Adhesion, Adsorption, Animal model, Apical chambers, Apposition, Berne, Biofunctionalized, Biofunctionalized implant surfaces, Biomaterials, Biomechanical, Biomechanical evaluation, Biomed, Biomed mater, Biomedical materials research, Blood plasma, Bone apposition, Bone area, Bone chamber, Bone chamber implants, Bone chambers, Bone formation, Bone matrix, Bone trabeculae, Bony ingrowth, Bony wall, Broggini, Bronectin, Bronectin fragment, Buser, Cell adhesion, Cell adhesion molecules, Cell attachment, Cochran, Coronal chambers, Current investigation, Dental implants, Different concentrations, Early stages, Experimental implants, Extracellular matrix, Hepes, Histomorphometric, Histomorphometric analysis, Histomorphometric study, Hydraulic actuator, Implant, Implant surface, Implant surface polymer coating, Implant types, Individual animals, Individual variability, Initial analysis, Institut straumann, Interfacial shear strength, Interfacial stiffness, Krsr, Krsr modsla, Lower container, Mater, Matrix, Maxilla, Maximum torque, Mineralized, Mineralized bone, Mineralized bone matrix, Mineralized tissue, Miniature pigs, Modsla, Modsla surfaces, Molecular architecture, Molecular weight, Molecular weight lysine unit, Native bone, Novel peptides, Osteoblast, Osteoblast attachment, Osteoblast differentiation, Osteoid, Peptide, Peptide sequence, Peptide sequences, Peptide surface density, Pmol, Pmol krsr, Pmol krsr pmol, Pmol pmol, Polymer, Potential differences, Present study, Previous studies, Protein adsorption, Rapid wound healing kinetics, Removal torque, Removal torque testing, Removal torque values, Room temperature, Salt buffer solution, Sandblasted, Shear strength, Similar outcome, Square interface, Standard deviation, Surface coverage, Surface density values, Surface topography, Surgical, Surgical research unit, Textor, Titanium, Titanium implant surfaces, Titanium implants, Titanium surface, Titanium surfaces, Torque, Tosatti, Treatment modalities, Treatment types, Wieland, Wiley periodicals.
- Teeft :
- Active peptides, Additional improvement, Adhesion, Adsorption, Animal model, Apical chambers, Apposition, Berne, Biofunctionalized, Biofunctionalized implant surfaces, Biomaterials, Biomechanical, Biomechanical evaluation, Biomed, Biomed mater, Biomedical materials research, Blood plasma, Bone apposition, Bone area, Bone chamber, Bone chamber implants, Bone chambers, Bone formation, Bone matrix, Bone trabeculae, Bony ingrowth, Bony wall, Broggini, Bronectin, Bronectin fragment, Buser, Cell adhesion, Cell adhesion molecules, Cell attachment, Cochran, Coronal chambers, Current investigation, Dental implants, Different concentrations, Early stages, Experimental implants, Extracellular matrix, Hepes, Histomorphometric, Histomorphometric analysis, Histomorphometric study, Hydraulic actuator, Implant, Implant surface, Implant surface polymer coating, Implant types, Individual animals, Individual variability, Initial analysis, Institut straumann, Interfacial shear strength, Interfacial stiffness, Krsr, Krsr modsla, Lower container, Mater, Matrix, Maxilla, Maximum torque, Mineralized, Mineralized bone, Mineralized bone matrix, Mineralized tissue, Miniature pigs, Modsla, Modsla surfaces, Molecular architecture, Molecular weight, Molecular weight lysine unit, Native bone, Novel peptides, Osteoblast, Osteoblast attachment, Osteoblast differentiation, Osteoid, Peptide, Peptide sequence, Peptide sequences, Peptide surface density, Pmol, Pmol krsr, Pmol krsr pmol, Pmol pmol, Polymer, Potential differences, Present study, Previous studies, Protein adsorption, Rapid wound healing kinetics, Removal torque, Removal torque testing, Removal torque values, Room temperature, Salt buffer solution, Sandblasted, Shear strength, Similar outcome, Square interface, Standard deviation, Surface coverage, Surface density values, Surface topography, Surgical, Surgical research unit, Textor, Titanium, Titanium implant surfaces, Titanium implants, Titanium surface, Titanium surfaces, Torque, Tosatti, Treatment modalities, Treatment types, Wieland, Wiley periodicals.
Abstract
Enhancing osseointegration through surface immobilization of multiple short peptide sequences that mimic extracellular matrix (ECM) proteins, such as arginine–glycine–aspartic acid (RGD) and lysine–arginine–serine–arginine (KRSR), has not yet been extensively explored. Additionally, the effect of biofunctionalizing chemically modified sandblasted and acid‐etched surfaces (modSLA) is unknown. The present study evaluated modSLA implant surfaces modified with RGD and KRSR for potentially enhanced effects on bone apposition and interfacial shear strength during early stages of bone regeneration. Two sets of experimental implants were placed in the maxillae of eight miniature pigs, known for their rapid wound healing kinetics: bone chamber implants creating two circular bone defects for histomorphometric analysis on one side and standard thread configuration implants for removal torque testing on the other side. Three different biofunctionalized modSLA surfaces using poly‐L‐lysine‐graft‐poly(ethylene glycol) (PLL‐g‐PEG) as a carrier minimizing nonspecific protein adsorption [(i) 20 pmol cm−2 KRSR alone (KRSR); or in combination with RGD in two different concentrations; (ii) 0.05 pmol cm−2 RGD (KRSR/RGD‐1); (iii) 1.26 pmol cm−2 RGD (KRSR/RGD‐2)] were compared with (iv) control modSLA. Animals were sacrificed at 2 weeks. Removal torque values (701.48–780.28 N mm), bone‐to‐implant contact (BIC) (35.22%–41.49%), and new bone fill (28.58%–30.62%) demonstrated no significant differences among treatments. It may be concluded that biofunctionalizing modSLA surfaces with KRSR and RGD derivatives of PLL‐g‐PEG polymer does not increase BIC, bone fill, or interfacial shear strength. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Url:
DOI: 10.1002/jbm.a.34004
Links to Exploration step
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Active peptides</term>
<term>Additional improvement</term>
<term>Adhesion</term>
<term>Adsorption</term>
<term>Animal model</term>
<term>Apical chambers</term>
<term>Apposition</term>
<term>Berne</term>
<term>Biofunctionalized</term>
<term>Biofunctionalized implant surfaces</term>
<term>Biomaterials</term>
<term>Biomechanical</term>
<term>Biomechanical evaluation</term>
<term>Biomed</term>
<term>Biomed mater</term>
<term>Biomedical materials research</term>
<term>Blood plasma</term>
<term>Bone apposition</term>
<term>Bone area</term>
<term>Bone chamber</term>
<term>Bone chamber implants</term>
<term>Bone chambers</term>
<term>Bone formation</term>
<term>Bone matrix</term>
<term>Bone trabeculae</term>
<term>Bony ingrowth</term>
<term>Bony wall</term>
<term>Broggini</term>
<term>Bronectin</term>
<term>Bronectin fragment</term>
<term>Buser</term>
<term>Cell adhesion</term>
<term>Cell adhesion molecules</term>
<term>Cell attachment</term>
<term>Cochran</term>
<term>Coronal chambers</term>
<term>Current investigation</term>
<term>Dental implants</term>
<term>Different concentrations</term>
<term>Early stages</term>
<term>Experimental implants</term>
<term>Extracellular matrix</term>
<term>Hepes</term>
<term>Histomorphometric</term>
<term>Histomorphometric analysis</term>
<term>Histomorphometric study</term>
<term>Hydraulic actuator</term>
<term>Implant</term>
<term>Implant surface</term>
<term>Implant surface polymer coating</term>
<term>Implant types</term>
<term>Individual animals</term>
<term>Individual variability</term>
<term>Initial analysis</term>
<term>Institut straumann</term>
<term>Interfacial shear strength</term>
<term>Interfacial stiffness</term>
<term>Krsr</term>
<term>Krsr modsla</term>
<term>Lower container</term>
<term>Mater</term>
<term>Matrix</term>
<term>Maxilla</term>
<term>Maximum torque</term>
<term>Mineralized</term>
<term>Mineralized bone</term>
<term>Mineralized bone matrix</term>
<term>Mineralized tissue</term>
<term>Miniature pigs</term>
<term>Modsla</term>
<term>Modsla surfaces</term>
<term>Molecular architecture</term>
<term>Molecular weight</term>
<term>Molecular weight lysine unit</term>
<term>Native bone</term>
<term>Novel peptides</term>
<term>Osteoblast</term>
<term>Osteoblast attachment</term>
<term>Osteoblast differentiation</term>
<term>Osteoid</term>
<term>Peptide</term>
<term>Peptide sequence</term>
<term>Peptide sequences</term>
<term>Peptide surface density</term>
<term>Pmol</term>
<term>Pmol krsr</term>
<term>Pmol krsr pmol</term>
<term>Pmol pmol</term>
<term>Polymer</term>
<term>Potential differences</term>
<term>Present study</term>
<term>Previous studies</term>
<term>Protein adsorption</term>
<term>Rapid wound healing kinetics</term>
<term>Removal torque</term>
<term>Removal torque testing</term>
<term>Removal torque values</term>
<term>Room temperature</term>
<term>Salt buffer solution</term>
<term>Sandblasted</term>
<term>Shear strength</term>
<term>Similar outcome</term>
<term>Square interface</term>
<term>Standard deviation</term>
<term>Surface coverage</term>
<term>Surface density values</term>
<term>Surface topography</term>
<term>Surgical</term>
<term>Surgical research unit</term>
<term>Textor</term>
<term>Titanium</term>
<term>Titanium implant surfaces</term>
<term>Titanium implants</term>
<term>Titanium surface</term>
<term>Titanium surfaces</term>
<term>Torque</term>
<term>Tosatti</term>
<term>Treatment modalities</term>
<term>Treatment types</term>
<term>Wieland</term>
<term>Wiley periodicals</term>
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<term>Additional improvement</term>
<term>Adhesion</term>
<term>Adsorption</term>
<term>Animal model</term>
<term>Apical chambers</term>
<term>Apposition</term>
<term>Berne</term>
<term>Biofunctionalized</term>
<term>Biofunctionalized implant surfaces</term>
<term>Biomaterials</term>
<term>Biomechanical</term>
<term>Biomechanical evaluation</term>
<term>Biomed</term>
<term>Biomed mater</term>
<term>Biomedical materials research</term>
<term>Blood plasma</term>
<term>Bone apposition</term>
<term>Bone area</term>
<term>Bone chamber</term>
<term>Bone chamber implants</term>
<term>Bone chambers</term>
<term>Bone formation</term>
<term>Bone matrix</term>
<term>Bone trabeculae</term>
<term>Bony ingrowth</term>
<term>Bony wall</term>
<term>Broggini</term>
<term>Bronectin</term>
<term>Bronectin fragment</term>
<term>Buser</term>
<term>Cell adhesion</term>
<term>Cell adhesion molecules</term>
<term>Cell attachment</term>
<term>Cochran</term>
<term>Coronal chambers</term>
<term>Current investigation</term>
<term>Dental implants</term>
<term>Different concentrations</term>
<term>Early stages</term>
<term>Experimental implants</term>
<term>Extracellular matrix</term>
<term>Hepes</term>
<term>Histomorphometric</term>
<term>Histomorphometric analysis</term>
<term>Histomorphometric study</term>
<term>Hydraulic actuator</term>
<term>Implant</term>
<term>Implant surface</term>
<term>Implant surface polymer coating</term>
<term>Implant types</term>
<term>Individual animals</term>
<term>Individual variability</term>
<term>Initial analysis</term>
<term>Institut straumann</term>
<term>Interfacial shear strength</term>
<term>Interfacial stiffness</term>
<term>Krsr</term>
<term>Krsr modsla</term>
<term>Lower container</term>
<term>Mater</term>
<term>Matrix</term>
<term>Maxilla</term>
<term>Maximum torque</term>
<term>Mineralized</term>
<term>Mineralized bone</term>
<term>Mineralized bone matrix</term>
<term>Mineralized tissue</term>
<term>Miniature pigs</term>
<term>Modsla</term>
<term>Modsla surfaces</term>
<term>Molecular architecture</term>
<term>Molecular weight</term>
<term>Molecular weight lysine unit</term>
<term>Native bone</term>
<term>Novel peptides</term>
<term>Osteoblast</term>
<term>Osteoblast attachment</term>
<term>Osteoblast differentiation</term>
<term>Osteoid</term>
<term>Peptide</term>
<term>Peptide sequence</term>
<term>Peptide sequences</term>
<term>Peptide surface density</term>
<term>Pmol</term>
<term>Pmol krsr</term>
<term>Pmol krsr pmol</term>
<term>Pmol pmol</term>
<term>Polymer</term>
<term>Potential differences</term>
<term>Present study</term>
<term>Previous studies</term>
<term>Protein adsorption</term>
<term>Rapid wound healing kinetics</term>
<term>Removal torque</term>
<term>Removal torque testing</term>
<term>Removal torque values</term>
<term>Room temperature</term>
<term>Salt buffer solution</term>
<term>Sandblasted</term>
<term>Shear strength</term>
<term>Similar outcome</term>
<term>Square interface</term>
<term>Standard deviation</term>
<term>Surface coverage</term>
<term>Surface density values</term>
<term>Surface topography</term>
<term>Surgical</term>
<term>Surgical research unit</term>
<term>Textor</term>
<term>Titanium</term>
<term>Titanium implant surfaces</term>
<term>Titanium implants</term>
<term>Titanium surface</term>
<term>Titanium surfaces</term>
<term>Torque</term>
<term>Tosatti</term>
<term>Treatment modalities</term>
<term>Treatment types</term>
<term>Wieland</term>
<term>Wiley periodicals</term>
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<front><div type="abstract">Enhancing osseointegration through surface immobilization of multiple short peptide sequences that mimic extracellular matrix (ECM) proteins, such as arginine–glycine–aspartic acid (RGD) and lysine–arginine–serine–arginine (KRSR), has not yet been extensively explored. Additionally, the effect of biofunctionalizing chemically modified sandblasted and acid‐etched surfaces (modSLA) is unknown. The present study evaluated modSLA implant surfaces modified with RGD and KRSR for potentially enhanced effects on bone apposition and interfacial shear strength during early stages of bone regeneration. Two sets of experimental implants were placed in the maxillae of eight miniature pigs, known for their rapid wound healing kinetics: bone chamber implants creating two circular bone defects for histomorphometric analysis on one side and standard thread configuration implants for removal torque testing on the other side. Three different biofunctionalized modSLA surfaces using poly‐L‐lysine‐graft‐poly(ethylene glycol) (PLL‐g‐PEG) as a carrier minimizing nonspecific protein adsorption [(i) 20 pmol cm−2 KRSR alone (KRSR); or in combination with RGD in two different concentrations; (ii) 0.05 pmol cm−2 RGD (KRSR/RGD‐1); (iii) 1.26 pmol cm−2 RGD (KRSR/RGD‐2)] were compared with (iv) control modSLA. Animals were sacrificed at 2 weeks. Removal torque values (701.48–780.28 N mm), bone‐to‐implant contact (BIC) (35.22%–41.49%), and new bone fill (28.58%–30.62%) demonstrated no significant differences among treatments. It may be concluded that biofunctionalizing modSLA surfaces with KRSR and RGD derivatives of PLL‐g‐PEG polymer does not increase BIC, bone fill, or interfacial shear strength. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.</div>
</front>
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<p>Enhancing osseointegration through surface immobilization of multiple short peptide sequences that mimic extracellular matrix (ECM) proteins, such as arginine–glycine–aspartic acid (RGD) and lysine–arginine–serine–arginine (KRSR), has not yet been extensively explored. Additionally, the effect of biofunctionalizing chemically modified sandblasted and acid‐etched surfaces (modSLA) is unknown. The present study evaluated modSLA implant surfaces modified with RGD and KRSR for potentially enhanced effects on bone apposition and interfacial shear strength during early stages of bone regeneration. Two sets of experimental implants were placed in the maxillae of eight miniature pigs, known for their rapid wound healing kinetics: bone chamber implants creating two circular bone defects for histomorphometric analysis on one side and standard thread configuration implants for removal torque testing on the other side. Three different biofunctionalized modSLA surfaces using poly‐L‐lysine‐<hi rend="italic">graft</hi>
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<correspondenceTo>Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Berne, Freiburgstrasse 7, Berne CH‐3010, Switzerland</correspondenceTo>
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<titleGroup><title type="main" xml:lang="en">Evaluation of chemically modified SLA implants (modSLA) biofunctionalized with integrin (RGD)‐ and heparin (KRSR)‐binding peptides<link href="#fn6"></link>
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<title type="short" xml:lang="en">Histomorphometric and Biomechanical Evaluation of Biofunctionalized Implant Surfaces</title>
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<creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Nina</givenNames>
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<creator xml:id="au2" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Samuele</givenNames>
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<keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">extracellular matrix protein binding sequences</keyword>
<keyword xml:id="kwd2">titanium surfaces biofunctionalized with biologically active peptides</keyword>
<keyword xml:id="kwd3">chemically modified sandblasted and acid‐etched titanium implant surfaces</keyword>
<keyword xml:id="kwd4">removal torque value</keyword>
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<p>Enhancing osseointegration through surface immobilization of multiple short peptide sequences that mimic extracellular matrix (ECM) proteins, such as arginine–glycine–aspartic acid (RGD) and lysine–arginine–serine–arginine (KRSR), has not yet been extensively explored. Additionally, the effect of biofunctionalizing chemically modified sandblasted and acid‐etched surfaces (modSLA) is unknown. The present study evaluated modSLA implant surfaces modified with RGD and KRSR for potentially enhanced effects on bone apposition and interfacial shear strength during early stages of bone regeneration. Two sets of experimental implants were placed in the maxillae of eight miniature pigs, known for their rapid wound healing kinetics: bone chamber implants creating two circular bone defects for histomorphometric analysis on one side and standard thread configuration implants for removal torque testing on the other side. Three different biofunctionalized modSLA surfaces using poly‐L‐lysine‐<i>graft</i>
‐poly(ethylene glycol) (PLL‐<i>g</i>
‐PEG) as a carrier minimizing nonspecific protein adsorption [(i) 20 pmol cm<sup>−2</sup>
KRSR alone (<b>KRSR</b>
); or in combination with RGD in two different concentrations; (ii) 0.05 pmol cm<sup>−2</sup>
RGD (<b>KRSR/RGD‐1</b>
); (iii) 1.26 pmol cm<sup>−2</sup>
RGD (<b>KRSR/RGD‐2</b>
)] were compared with (iv) control <b>modSLA</b>
. Animals were sacrificed at 2 weeks. Removal torque values (701.48–780.28 N mm), bone‐to‐implant contact (BIC) (35.22%–41.49%), and new bone fill (28.58%–30.62%) demonstrated no significant differences among treatments. It may be concluded that biofunctionalizing modSLA surfaces with KRSR and RGD derivatives of PLL‐<i>g</i>
‐PEG polymer does not increase BIC, bone fill, or interfacial shear strength. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.</p>
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<note xml:id="fn7"><p><b>How to cite this article</b>
: Broggini N, Tosatti S, Ferguson SJ, Schuler M, Textor M, Bornstein MM, Bosshardt DD, Buser D. 2012. Evaluation of chemically modified SLA implants (modSLA) biofunctionalized with integrin (RGD)‐ and heparin (KRSR)‐binding peptides. J Biomed Mater Res Part A 2012:100A:703–711.</p>
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<abstract>Enhancing osseointegration through surface immobilization of multiple short peptide sequences that mimic extracellular matrix (ECM) proteins, such as arginine–glycine–aspartic acid (RGD) and lysine–arginine–serine–arginine (KRSR), has not yet been extensively explored. Additionally, the effect of biofunctionalizing chemically modified sandblasted and acid‐etched surfaces (modSLA) is unknown. The present study evaluated modSLA implant surfaces modified with RGD and KRSR for potentially enhanced effects on bone apposition and interfacial shear strength during early stages of bone regeneration. Two sets of experimental implants were placed in the maxillae of eight miniature pigs, known for their rapid wound healing kinetics: bone chamber implants creating two circular bone defects for histomorphometric analysis on one side and standard thread configuration implants for removal torque testing on the other side. Three different biofunctionalized modSLA surfaces using poly‐L‐lysine‐graft‐poly(ethylene glycol) (PLL‐g‐PEG) as a carrier minimizing nonspecific protein adsorption [(i) 20 pmol cm−2 KRSR alone (KRSR); or in combination with RGD in two different concentrations; (ii) 0.05 pmol cm−2 RGD (KRSR/RGD‐1); (iii) 1.26 pmol cm−2 RGD (KRSR/RGD‐2)] were compared with (iv) control modSLA. Animals were sacrificed at 2 weeks. Removal torque values (701.48–780.28 N mm), bone‐to‐implant contact (BIC) (35.22%–41.49%), and new bone fill (28.58%–30.62%) demonstrated no significant differences among treatments. It may be concluded that biofunctionalizing modSLA surfaces with KRSR and RGD derivatives of PLL‐g‐PEG polymer does not increase BIC, bone fill, or interfacial shear strength. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.</abstract>
<note type="content">*No benefit of any kind will be received either directly or indirectly by the author(s). Company affiliation took place with one author only after the study was completed.</note>
<note type="content">*How to cite this article: Broggini N, Tosatti S, Ferguson SJ, Schuler M, Textor M, Bornstein MM, Bosshardt DD, Buser D. 2012. Evaluation of chemically modified SLA implants (modSLA) biofunctionalized with integrin (RGD)‐ and heparin (KRSR)‐binding peptides. J Biomed Mater Res Part A 2012:100A:703–711.</note>
<note type="funding">ITI Foundation for the Promotion of Oral Implantology, Basel, Switzerland - No. 459_2006; </note>
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<topic>removal torque value</topic>
<topic>histomorphometry</topic>
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