Biological behavior of neurally differentiated periodontal ligament stem cells on different titanium implant surfaces
Identifieur interne : 000244 ( Istex/Checkpoint ); précédent : 000243; suivant : 000245Biological behavior of neurally differentiated periodontal ligament stem cells on different titanium implant surfaces
Auteurs : Li Xiaojie [République populaire de Chine] ; Liao Dapeng [République populaire de Chine] ; Gong Ping [République populaire de Chine] ; Dong Yan [République populaire de Chine] ; Sun Gang [République populaire de Chine]Source :
- Journal of Biomedical Materials Research Part A [ 1549-3296 ] ; 2014-08.
Abstract
We investigated the biological behavior of periodontal ligament stem cells (PDLSCs) induced to differentiate into Schwann cells (SCs) on the surfaces of titanium discs. Two types of titanium surfaces, sand blasted and acid etched (SA) and smooth polished, were prepared on titanium discs, and the behavior of SC‐like cells on these discs was investigated. Cell morphology was examined by scanning electron microscopy, and cell proliferation was assessed using assays for methylthiazol tetrazolium metabolism and total protein content. Real‐time polymerase chain reaction and Western blotting analyses were used to determine the gene and protein expression, respectively, of SC markers by PDLSCs. Differentiated PDLSCs could adhere, proliferate, differentiate, and express SC marker genes and proteins on the prepared titanium surfaces, and the highest levels of SC marker protein expression were observed in PDLSCs on SA titanium surfaces. SA titanium surfaces show good biocompatibility for the SC‐like cells, which is important for the application of these cells in peri‐implant nerve tissue engineering, through which they are expected to improve the osseoperception of dental implants. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2805–2812, 2014.
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DOI: 10.1002/jbm.a.34953
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uniqKey="Ping G" first="Gong" last="Ping">Gong Ping</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Dental Implant Center, West China College of Stomatology, Sichuan University, Sichuan, Chengdu</wicri:regionArea><wicri:noRegion>Chengdu</wicri:noRegion></affiliation></author><author><name sortKey="Yan, Dong" sort="Yan, Dong" uniqKey="Yan D" first="Dong" last="Yan">Dong Yan</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Dentistry, Second Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region><settlement type="city">Hangzhou</settlement></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation></author><author><name sortKey="Gang, Sun" sort="Gang, Sun" uniqKey="Gang S" first="Sun" last="Gang">Sun Gang</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Dentistry, Second Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region><settlement type="city">Hangzhou</settlement></placeName><orgName type="university">Université de Zhejiang</orgName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Biomedical Materials Research Part A</title><title level="j" type="alt">JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A</title><idno type="ISSN">1549-3296</idno><idno type="eISSN">1552-4965</idno><imprint><biblScope unit="vol">102</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="2805">2805</biblScope><biblScope unit="page" to="2812">2812</biblScope><biblScope unit="page-count">8</biblScope><date type="published" when="2014-08">2014-08</date></imprint><idno type="ISSN">1549-3296</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1549-3296</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">We investigated the biological behavior of periodontal ligament stem cells (PDLSCs) induced to differentiate into Schwann cells (SCs) on the surfaces of titanium discs. Two types of titanium surfaces, sand blasted and acid etched (SA) and smooth polished, were prepared on titanium discs, and the behavior of SC‐like cells on these discs was investigated. Cell morphology was examined by scanning electron microscopy, and cell proliferation was assessed using assays for methylthiazol tetrazolium metabolism and total protein content. Real‐time polymerase chain reaction and Western blotting analyses were used to determine the gene and protein expression, respectively, of SC markers by PDLSCs. Differentiated PDLSCs could adhere, proliferate, differentiate, and express SC marker genes and proteins on the prepared titanium surfaces, and the highest levels of SC marker protein expression were observed in PDLSCs on SA titanium surfaces. SA titanium surfaces show good biocompatibility for the SC‐like cells, which is important for the application of these cells in peri‐implant nerve tissue engineering, through which they are expected to improve the osseoperception of dental implants. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2805–2812, 2014.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement><orgName><li>Université de Zhejiang</li></orgName></list><tree><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Xiaojie, Li" sort="Xiaojie, Li" uniqKey="Xiaojie L" first="Li" last="Xiaojie">Li Xiaojie</name></region><name sortKey="Dapeng, Liao" sort="Dapeng, Liao" uniqKey="Dapeng L" first="Liao" last="Dapeng">Liao Dapeng</name><name sortKey="Gang, Sun" sort="Gang, Sun" uniqKey="Gang S" first="Sun" last="Gang">Sun Gang</name><name sortKey="Ping, Gong" sort="Ping, Gong" uniqKey="Ping G" first="Gong" last="Ping">Gong Ping</name><name sortKey="Yan, Dong" sort="Yan, Dong" uniqKey="Yan D" first="Dong" last="Yan">Dong Yan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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