Bacterial profiles of saliva in relation to diet, lifestyle factors, and socioeconomic status
Identifieur interne : 000697 ( Pmc/Corpus ); précédent : 000696; suivant : 000698Bacterial profiles of saliva in relation to diet, lifestyle factors, and socioeconomic status
Auteurs : Daniel Belstr M ; Palle Holmstrup ; Claus H. Nielsen ; Nikolai Kirkby ; Svante Twetman ; Berit L. Heitmann ; Vanja Klepac-Ceraj ; Bruce J. Paster ; Nils-Erik FiehnSource :
- Journal of Oral Microbiology [ 2000-2297 ] ; 2014.
Abstract
The bacterial profile of saliva is composed of bacteria from different oral surfaces. The objective of this study was to determine whether different diet intake, lifestyle, or socioeconomic status is associated with characteristic bacterial saliva profiles.
Stimulated saliva samples from 292 participants with low levels of dental caries and periodontitis, enrolled in the Danish Health Examination Survey (DANHES), were analyzed for the presence of approximately 300 bacterial species by means of the Human Oral Microbe Identification Microarray (HOMIM). Using presence and levels (mean HOMIM-value) of bacterial probes as endpoints, the influence of diet intake, lifestyle, and socioeconomic status on the bacterial saliva profile was analyzed by Mann–Whitney tests with Benjamini–Hochberg's correction for multiple comparisons and principal component analysis.
Targets for 131 different probes were identified in 292 samples, with
The bacterial profile of saliva seems independent of diet intake, but influenced by smoking and maybe socioeconomic status.
Url:
DOI: 10.3402/jom.v6.23609
PubMed: 24765243
PubMed Central: 3974179
Links to Exploration step
PMC:3974179Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Bacterial profiles of saliva in relation to diet, lifestyle factors, and socioeconomic status</title><author><name sortKey="Belstr M, Daniel" sort="Belstr M, Daniel" uniqKey="Belstr M D" first="Daniel" last="Belstr M">Daniel Belstr M</name><affiliation><nlm:aff id="AF0001">Section of Periodontology, School of Dentistry, Faculty of Health Science, University of Copenhagen, Denmark</nlm:aff></affiliation></author><author><name sortKey="Holmstrup, Palle" sort="Holmstrup, Palle" uniqKey="Holmstrup P" first="Palle" last="Holmstrup">Palle Holmstrup</name><affiliation><nlm:aff id="AF0001">Section of Periodontology, School of Dentistry, Faculty of Health Science, University of Copenhagen, Denmark</nlm:aff></affiliation></author><author><name sortKey="Nielsen, Claus H" sort="Nielsen, Claus H" uniqKey="Nielsen C" first="Claus H." last="Nielsen">Claus H. Nielsen</name><affiliation><nlm:aff id="AF0002">Institute for Inflammation Research, Department of Infectious Diseases and Rheumatology, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark</nlm:aff></affiliation></author><author><name sortKey="Kirkby, Nikolai" sort="Kirkby, Nikolai" uniqKey="Kirkby N" first="Nikolai" last="Kirkby">Nikolai Kirkby</name><affiliation><nlm:aff id="AF0003">Department of Medical Microbiology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark</nlm:aff></affiliation></author><author><name sortKey="Twetman, Svante" sort="Twetman, Svante" uniqKey="Twetman S" first="Svante" last="Twetman">Svante Twetman</name><affiliation><nlm:aff id="AF0004">Section of Cariology, School of Dentistry, Faculty of Health Science, University of Copenhagen, Denmark</nlm:aff></affiliation></author><author><name sortKey="Heitmann, Berit L" sort="Heitmann, Berit L" uniqKey="Heitmann B" first="Berit L." last="Heitmann">Berit L. Heitmann</name><affiliation><nlm:aff id="AF0005">Institute of Preventive Medicine, Bispebejerg and Frederiksberg Hospitals, The Capital Region, Copenhagen, Denmark</nlm:aff></affiliation><affiliation><nlm:aff id="AF0006">The Boden Institute of Obesity, Nutrition, Exercise & Eating Disorders, University of Sydney, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="AF0007">National Institute of Public Health, University of Southern Denmark, Copenhagen, Denmark</nlm:aff></affiliation></author><author><name sortKey="Klepac Ceraj, Vanja" sort="Klepac Ceraj, Vanja" uniqKey="Klepac Ceraj V" first="Vanja" last="Klepac-Ceraj">Vanja Klepac-Ceraj</name><affiliation><nlm:aff id="AF0008">Department of Biological Sciences, Wellesley College, Wellesley, MA, USA</nlm:aff></affiliation></author><author><name sortKey="Paster, Bruce J" sort="Paster, Bruce J" uniqKey="Paster B" first="Bruce J." last="Paster">Bruce J. Paster</name><affiliation><nlm:aff id="AF0009">Department of Microbiology, The Forsyth Institute, Cambridge, MA, USA</nlm:aff></affiliation><affiliation><nlm:aff id="AF0010">Department of Oral Medicine, Infection & Immunity, Harvard School of Dental Medicine, Boston, MA, USA</nlm:aff></affiliation></author><author><name sortKey="Fiehn, Nils Erik" sort="Fiehn, Nils Erik" uniqKey="Fiehn N" first="Nils-Erik" last="Fiehn">Nils-Erik Fiehn</name><affiliation><nlm:aff id="AF0011">Department of International Health, Immunology & Microbiology, University of Copenhagen, Copenhagen, Denmark</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24765243</idno><idno type="pmc">3974179</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974179</idno><idno type="RBID">PMC:3974179</idno><idno type="doi">10.3402/jom.v6.23609</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000697</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000697</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Bacterial profiles of saliva in relation to diet, lifestyle factors, and socioeconomic status</title><author><name sortKey="Belstr M, Daniel" sort="Belstr M, Daniel" uniqKey="Belstr M D" first="Daniel" last="Belstr M">Daniel Belstr M</name><affiliation><nlm:aff id="AF0001">Section of Periodontology, School of Dentistry, Faculty of Health Science, University of Copenhagen, Denmark</nlm:aff></affiliation></author><author><name sortKey="Holmstrup, Palle" sort="Holmstrup, Palle" uniqKey="Holmstrup P" first="Palle" last="Holmstrup">Palle Holmstrup</name><affiliation><nlm:aff id="AF0001">Section of Periodontology, School of Dentistry, Faculty of Health Science, University of Copenhagen, Denmark</nlm:aff></affiliation></author><author><name sortKey="Nielsen, Claus H" sort="Nielsen, Claus H" uniqKey="Nielsen C" first="Claus H." last="Nielsen">Claus H. Nielsen</name><affiliation><nlm:aff id="AF0002">Institute for Inflammation Research, Department of Infectious Diseases and Rheumatology, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark</nlm:aff></affiliation></author><author><name sortKey="Kirkby, Nikolai" sort="Kirkby, Nikolai" uniqKey="Kirkby N" first="Nikolai" last="Kirkby">Nikolai Kirkby</name><affiliation><nlm:aff id="AF0003">Department of Medical Microbiology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark</nlm:aff></affiliation></author><author><name sortKey="Twetman, Svante" sort="Twetman, Svante" uniqKey="Twetman S" first="Svante" last="Twetman">Svante Twetman</name><affiliation><nlm:aff id="AF0004">Section of Cariology, School of Dentistry, Faculty of Health Science, University of Copenhagen, Denmark</nlm:aff></affiliation></author><author><name sortKey="Heitmann, Berit L" sort="Heitmann, Berit L" uniqKey="Heitmann B" first="Berit L." last="Heitmann">Berit L. Heitmann</name><affiliation><nlm:aff id="AF0005">Institute of Preventive Medicine, Bispebejerg and Frederiksberg Hospitals, The Capital Region, Copenhagen, Denmark</nlm:aff></affiliation><affiliation><nlm:aff id="AF0006">The Boden Institute of Obesity, Nutrition, Exercise & Eating Disorders, University of Sydney, Australia</nlm:aff></affiliation><affiliation><nlm:aff id="AF0007">National Institute of Public Health, University of Southern Denmark, Copenhagen, Denmark</nlm:aff></affiliation></author><author><name sortKey="Klepac Ceraj, Vanja" sort="Klepac Ceraj, Vanja" uniqKey="Klepac Ceraj V" first="Vanja" last="Klepac-Ceraj">Vanja Klepac-Ceraj</name><affiliation><nlm:aff id="AF0008">Department of Biological Sciences, Wellesley College, Wellesley, MA, USA</nlm:aff></affiliation></author><author><name sortKey="Paster, Bruce J" sort="Paster, Bruce J" uniqKey="Paster B" first="Bruce J." last="Paster">Bruce J. Paster</name><affiliation><nlm:aff id="AF0009">Department of Microbiology, The Forsyth Institute, Cambridge, MA, USA</nlm:aff></affiliation><affiliation><nlm:aff id="AF0010">Department of Oral Medicine, Infection & Immunity, Harvard School of Dental Medicine, Boston, MA, USA</nlm:aff></affiliation></author><author><name sortKey="Fiehn, Nils Erik" sort="Fiehn, Nils Erik" uniqKey="Fiehn N" first="Nils-Erik" last="Fiehn">Nils-Erik Fiehn</name><affiliation><nlm:aff id="AF0011">Department of International Health, Immunology & Microbiology, University of Copenhagen, Copenhagen, Denmark</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Oral Microbiology</title><idno type="eISSN">2000-2297</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Background and objective</title><p>The bacterial profile of saliva is composed of bacteria from different oral surfaces. The objective of this study was to determine whether different diet intake, lifestyle, or socioeconomic status is associated with characteristic bacterial saliva profiles.</p></sec><sec id="st2"><title>Design</title><p>Stimulated saliva samples from 292 participants with low levels of dental caries and periodontitis, enrolled in the Danish Health Examination Survey (DANHES), were analyzed for the presence of approximately 300 bacterial species by means of the Human Oral Microbe Identification Microarray (HOMIM). Using presence and levels (mean HOMIM-value) of bacterial probes as endpoints, the influence of diet intake, lifestyle, and socioeconomic status on the bacterial saliva profile was analyzed by Mann–Whitney tests with Benjamini–Hochberg's correction for multiple comparisons and principal component analysis.</p></sec><sec id="st3"><title>Results</title><p>Targets for 131 different probes were identified in 292 samples, with <italic>Streptococcus</italic> and <italic>Veillonella</italic> being the most predominant genera identified. Two bacterial taxa (<italic>Streptococcus sobrinus</italic> and <italic>Eubacterium</italic> [11][G-3] <italic>brachy</italic>) were more associated with smokers than non-smokers (adjusted <italic>p-</italic>value<0.01). Stratification of the group based on extreme ends of the parameters age, gender, alcohol consumption, body mass index (BMI), and diet intake had no statistical influence on the composition of the bacterial profile of saliva. Conversely, differences in socioeconomic status were reflected by the bacterial profiles of saliva.</p></sec><sec id="st4"><title>Conclusions</title><p>The bacterial profile of saliva seems independent of diet intake, but influenced by smoking and maybe socioeconomic status.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Aas, Ja" uniqKey="Aas J">JA Aas</name></author><author><name sortKey="Paster, Bj" uniqKey="Paster B">BJ Paster</name></author><author><name sortKey="Stokes, Ln" uniqKey="Stokes L">LN Stokes</name></author><author><name sortKey="Olsen, I" uniqKey="Olsen I">I Olsen</name></author><author><name sortKey="Dewhirst, Fe" uniqKey="Dewhirst F">FE Dewhirst</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paster, Bj" uniqKey="Paster B">BJ Paster</name></author><author><name sortKey="Boches, Sk" uniqKey="Boches S">SK Boches</name></author><author><name sortKey="Galvin, Jl" 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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Oral Microbiol</journal-id><journal-id journal-id-type="iso-abbrev">J Oral Microbiol</journal-id><journal-id journal-id-type="publisher-id">JOM</journal-id><journal-title-group><journal-title>Journal of Oral Microbiology</journal-title></journal-title-group><issn pub-type="epub">2000-2297</issn><publisher><publisher-name>Co-Action Publishing</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24765243</article-id><article-id pub-id-type="pmc">3974179</article-id><article-id pub-id-type="publisher-id">23609</article-id><article-id pub-id-type="doi">10.3402/jom.v6.23609</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Bacterial profiles of saliva in relation to diet, lifestyle factors, and socioeconomic status</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Belstrøm</surname><given-names>Daniel</given-names></name><xref ref-type="aff" rid="AF0001">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Holmstrup</surname><given-names>Palle</given-names></name><xref ref-type="aff" rid="AF0001">1</xref></contrib><contrib contrib-type="author"><name><surname>Nielsen</surname><given-names>Claus H.</given-names></name><xref ref-type="aff" rid="AF0002">2</xref></contrib><contrib contrib-type="author"><name><surname>Kirkby</surname><given-names>Nikolai</given-names></name><xref ref-type="aff" rid="AF0003">3</xref></contrib><contrib contrib-type="author"><name><surname>Twetman</surname><given-names>Svante</given-names></name><xref ref-type="aff" rid="AF0004">4</xref></contrib><contrib contrib-type="author"><name><surname>Heitmann</surname><given-names>Berit L.</given-names></name><xref ref-type="aff" rid="AF0005">5</xref><xref ref-type="aff" rid="AF0006">6</xref><xref ref-type="aff" rid="AF0007">7</xref></contrib><contrib contrib-type="author"><name><surname>Klepac-Ceraj</surname><given-names>Vanja</given-names></name><xref ref-type="aff" rid="AF0008">8</xref></contrib><contrib contrib-type="author"><name><surname>Paster</surname><given-names>Bruce J.</given-names></name><xref ref-type="aff" rid="AF0009">9</xref><xref ref-type="aff" rid="AF0010">10</xref></contrib><contrib contrib-type="author"><name><surname>Fiehn</surname><given-names>Nils-Erik</given-names></name><xref ref-type="aff" rid="AF0011">11</xref></contrib></contrib-group><aff id="AF0001"><label>1</label>Section of Periodontology, School of Dentistry, Faculty of Health Science, University of Copenhagen, Denmark</aff><aff id="AF0002"><label>2</label>Institute for Inflammation Research, Department of Infectious Diseases and Rheumatology, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark</aff><aff id="AF0003"><label>3</label>Department of Medical Microbiology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark</aff><aff id="AF0004"><label>4</label>Section of Cariology, School of Dentistry, Faculty of Health Science, University of Copenhagen, Denmark</aff><aff id="AF0005"><label>5</label>Institute of Preventive Medicine, Bispebejerg and Frederiksberg Hospitals, The Capital Region, Copenhagen, Denmark</aff><aff id="AF0006"><label>6</label>The Boden Institute of Obesity, Nutrition, Exercise & Eating Disorders, University of Sydney, Australia</aff><aff id="AF0007"><label>7</label>National Institute of Public Health, University of Southern Denmark, Copenhagen, Denmark</aff><aff id="AF0008"><label>8</label>Department of Biological Sciences, Wellesley College, Wellesley, MA, USA</aff><aff id="AF0009"><label>9</label>Department of Microbiology, The Forsyth Institute, Cambridge, MA, USA</aff><aff id="AF0010"><label>10</label>Department of Oral Medicine, Infection & Immunity, Harvard School of Dental Medicine, Boston, MA, USA</aff><aff id="AF0011"><label>11</label>Department of International Health, Immunology & Microbiology, University of Copenhagen, Copenhagen, Denmark</aff><author-notes><corresp id="cor1"><label>*</label>Correspondence to: Daniel Belstrøm, Section of Periodontology, School of Dentistry, Faculty of Health Science, University of Copenhagen, Copenhagen, Denmark, Email: <email xlink:href="dbel@sund.ku.dk">dbel@sund.ku.dk</email></corresp></author-notes><pub-date pub-type="epub"><day>01</day><month>4</month><year>2014</year></pub-date><pub-date pub-type="collection"><year>2014</year></pub-date><volume>6</volume><elocation-id content-type="doi">10.3402/jom.v6.23609</elocation-id><history><date date-type="received"><day>18</day><month>12</month><year>2013</year></date><date date-type="rev-recd"><day>10</day><month>3</month><year>2014</year></date><date date-type="accepted"><day>13</day><month>3</month><year>2014</year></date></history><permissions><copyright-statement>© 2014 Daniel Belstrøm et al.</copyright-statement><copyright-year>2014</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-nd/3.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><sec id="st1"><title>Background and objective</title><p>The bacterial profile of saliva is composed of bacteria from different oral surfaces. The objective of this study was to determine whether different diet intake, lifestyle, or socioeconomic status is associated with characteristic bacterial saliva profiles.</p></sec><sec id="st2"><title>Design</title><p>Stimulated saliva samples from 292 participants with low levels of dental caries and periodontitis, enrolled in the Danish Health Examination Survey (DANHES), were analyzed for the presence of approximately 300 bacterial species by means of the Human Oral Microbe Identification Microarray (HOMIM). Using presence and levels (mean HOMIM-value) of bacterial probes as endpoints, the influence of diet intake, lifestyle, and socioeconomic status on the bacterial saliva profile was analyzed by Mann–Whitney tests with Benjamini–Hochberg's correction for multiple comparisons and principal component analysis.</p></sec><sec id="st3"><title>Results</title><p>Targets for 131 different probes were identified in 292 samples, with <italic>Streptococcus</italic> and <italic>Veillonella</italic> being the most predominant genera identified. Two bacterial taxa (<italic>Streptococcus sobrinus</italic> and <italic>Eubacterium</italic> [11][G-3] <italic>brachy</italic>) were more associated with smokers than non-smokers (adjusted <italic>p-</italic>value<0.01). Stratification of the group based on extreme ends of the parameters age, gender, alcohol consumption, body mass index (BMI), and diet intake had no statistical influence on the composition of the bacterial profile of saliva. Conversely, differences in socioeconomic status were reflected by the bacterial profiles of saliva.</p></sec><sec id="st4"><title>Conclusions</title><p>The bacterial profile of saliva seems independent of diet intake, but influenced by smoking and maybe socioeconomic status.</p></sec></abstract><kwd-group><kwd>saliva</kwd><kwd>HOMIM</kwd><kwd>bacteria</kwd><kwd>diet lifestyle</kwd></kwd-group></article-meta><notes><fn-group><fn><p>To access the supplementary material for this article, please see <ext-link ext-link-type="uri" xlink:href="http://www.journaloforalmicrobiology.net/index.php/jom/rt/suppFiles/23609/0">Supplementary Files</ext-link> under Article Tools online.</p></fn></fn-group></notes></front><body><p>The oral cavity is an open environment harboring a complex community comprising >700 different oral bacterial species, of which only around 50% have been cultivated (<xref rid="CIT0001" ref-type="bibr">1</xref>, <xref rid="CIT0002" ref-type="bibr">2</xref>). Over recent decades, molecular methods based on sequencing of the bacterial 16S ribosomal RNA (rRNA) gene have expanded the knowledge about oral bacteria considerably (<xref rid="CIT0003" ref-type="bibr">3</xref>). Thus, studies have revealed a hitherto unknown diversity of the oral microbiota, with significant variation between different oral locations (<xref rid="CIT0004" ref-type="bibr">4</xref>, <xref rid="CIT0005" ref-type="bibr">5</xref>). The resident oral microbiota is believed to play an essential role in maintaining oral homeostasis, protecting the oral cavity against the development of disease (<xref rid="CIT0003" ref-type="bibr">3</xref>). However, dental caries and periodontitis, the two most prevalent oral diseases, have been found to be associated with site-specific changes in the oral microbiota (<xref rid="CIT0006" ref-type="bibr">6</xref>, <xref rid="CIT0007" ref-type="bibr">7</xref>).</p><p>Differences in diet intake, lifestyle, and socioeconomic status are generally considered important factors in the multifactorial background of dental caries and periodontitis. Obesity often measured by a body mass index (BMI) above 30 kg/m<sup>2</sup> is associated with high levels of dental caries (<xref rid="CIT0008" ref-type="bibr">8</xref>) and periodontitis (<xref rid="CIT0009" ref-type="bibr">9</xref>, <xref rid="CIT0010" ref-type="bibr">10</xref>), and a high frequency of sugar intake increases the risk of dental caries (<xref rid="CIT0011" ref-type="bibr">11</xref>). Smoking increases the risk of acquiring periodontitis and of aggravating existing diseases (<xref rid="CIT0012" ref-type="bibr">12</xref>). High intake of alcohol has been suggested to protect against periodontitis (<xref rid="CIT0013" ref-type="bibr">13</xref>), as well as being a risk factor for periodontitis (<xref rid="CIT0014" ref-type="bibr">14</xref>). In addition, low socioeconomic status is also known to be associated with periodontitis (<xref rid="CIT0015" ref-type="bibr">15</xref>, <xref rid="CIT0016" ref-type="bibr">16</xref>) and dental caries (<xref rid="CIT0017" ref-type="bibr">17</xref>, <xref rid="CIT0018" ref-type="bibr">18</xref>). At present, little is known about the influence of differences in diet, lifestyle, and socioeconomic status on composition of the oral microbiome, and whether differences in bacterial composition may be detected in saliva samples.</p><p>Being an easily collectable, non-invasive biological material, saliva is suitable for medical investigation, and several health and disease-associated factors are reflected in saliva (<xref rid="CIT0019" ref-type="bibr">19</xref>). Thus, saliva is currently being thoroughly investigated in general and in dental medicine in the search for biomarkers of health and disease (<xref rid="CIT0020" ref-type="bibr">20</xref>–<xref rid="CIT0022" ref-type="bibr">22</xref>). A few studies have investigated the bacterial composition of saliva in oral health (<xref rid="CIT0005" ref-type="bibr">5</xref>, <xref rid="CIT0023" ref-type="bibr">23</xref>–<xref rid="CIT0025" ref-type="bibr">25</xref>) and verified that a salivary bacterial profile resembles that of the throat, tonsils, and dorsum of the tongue (<xref rid="CIT0026" ref-type="bibr">26</xref>). In addition, the salivary microbiome has been shown to be highly diverse and is proposed to depend on lifestyle and diet intake (<xref rid="CIT0025" ref-type="bibr">25</xref>, <xref rid="CIT0027" ref-type="bibr">27</xref>). Also, oral disease may influence the bacterial profile, and recently it has been shown that both dental caries and periodontitis are associated with characteristic bacterial profiles of saliva different from that of oral health (<xref rid="CIT0028" ref-type="bibr">28</xref>, <xref rid="CIT0029" ref-type="bibr">29</xref>).</p><p>In this study, we investigated stimulated saliva samples collected from 292 participants of the Danish Health Examination Survey (DANHES). We used the Human Oral Microbe Identification Microarray (HOMIM), a 16S rRNA high-throughput based microarray technique able to identify around 300 different bacterial species simultaneously. The purpose of the study was to determine whether differences in diet intake, lifestyle, and socioeconomic status associate with the bacterial profile of saliva.</p><sec sec-type="materials|methods" id="S0002"><title>Materials and methods</title><sec id="S0002-S20001"><title>Study population</title><p>The cohort of the Danish population-based study DANHES (<xref rid="CIT0030" ref-type="bibr">30</xref>) and the subpopulation participating in the oral part of the study (<xref rid="CIT0031" ref-type="bibr">31</xref>) have been described in detail elsewhere. In brief, the DANHES study was carried out in 13 different municipalities in 2007–2008, and oral examinations were performed in 2008–2009. A total of 76,484 individuals participated in the questionnaire survey concerning general health, and 18,065 had a general medical examination performed. In addition to the general medical examination, 4,402 subjects had a dental examination performed, of which 4,337 delivered a stimulated saliva sample and 4,230 answered a questionnaire concerning self-perceived oral health. All participants received written information prior to participation and signed informed consent. The study was approved by the regional ethical committee (H-C-2007-0118) and reported to the Danish data authority (2007-41-1567). This study is part of a group of studies conducted on saliva samples collected from the DANHES cohort, including studies investigating how periodontitis and dental caries associate with the bacterial profile of saliva (<xref rid="CIT0028" ref-type="bibr">28</xref>, <xref rid="CIT0029" ref-type="bibr">29</xref>). Population characteristics are presented in <xref ref-type="table" rid="T0001">Table 1</xref>, and a flowchart depicting the sample selection strategy is shown in <xref ref-type="fig" rid="F0001">Fig. 1</xref>. In this study, dental caries was defined as: untreated caries lesions ≥3 surfaces, and manifest periodontitis was defined as bleeding on probing (BOP) on ≥25% of total sites+minimum 2 teeth with clinical attachment level ≥4 mm+minimum 2 teeth with probing depth ≥6 mm. Finally, a total of 292 participants with low levels of oral diseases from 12 different municipalities were included in this study.</p><fig id="F0001" position="float"><label>Fig. 1</label><caption><p>Flowchart of sample selection and group establishment.</p></caption><graphic xlink:href="JOM-6-23609-g001"></graphic></fig><table-wrap id="T0001" position="float"><label>Table 1</label><caption><p>Population characteristics</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="1" colspan="1"></th><th align="center" rowspan="1" colspan="1">Mean (SD)</th><th align="center" rowspan="1" colspan="1">Range</th><th align="center" rowspan="1" colspan="1">Lower quintile <italic>n</italic>=58, mean (SD)</th><th align="center" rowspan="1" colspan="1">Upper quintile <italic>n</italic>=58, mean (SD)</th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1">Age (years)</td><td align="center" rowspan="1" colspan="1">55 (13)</td><td align="center" rowspan="1" colspan="1">20–81</td><td align="center" rowspan="1" colspan="1">35 (5)</td><td align="center" rowspan="1" colspan="1">71 (4)</td></tr><tr><td align="left" rowspan="1" colspan="1">BMI</td><td align="center" rowspan="1" colspan="1">25.1 (3.7)</td><td align="center" rowspan="1" colspan="1">17.8–38.4</td><td align="center" rowspan="1" colspan="1">20.4 (1.2)</td><td align="center" rowspan="1" colspan="1">30.6 (2.4)</td></tr><tr><td align="left" rowspan="1" colspan="1">Alcohol intake (units/week)</td><td align="center" rowspan="1" colspan="1">7.7 (7.2)</td><td align="center" rowspan="1" colspan="1">0–48</td><td align="center" rowspan="1" colspan="1">0.3 (0.6)</td><td align="center" rowspan="1" colspan="1">19.5 (6.5)</td></tr><tr><td align="left" rowspan="1" colspan="1">Energy intake from fat (%)</td><td align="center" rowspan="1" colspan="1">30.8 (9.8)</td><td align="center" rowspan="1" colspan="1">3.8–86.5</td><td align="center" rowspan="1" colspan="1">19.3 (4.6)</td><td align="center" rowspan="1" colspan="1">44.5 (10.6)</td></tr><tr><td align="left" rowspan="1" colspan="1">Energy intake from protein (%)</td><td align="center" rowspan="1" colspan="1">17.0 (4.2)</td><td align="center" rowspan="1" colspan="1">4.3–36.5</td><td align="center" rowspan="1" colspan="1">11.8 (1.8)</td><td align="center" rowspan="1" colspan="1">23.0 (3.8)</td></tr><tr><td align="left" rowspan="1" colspan="1">Energy intake from carbohydrates (%)</td><td align="center" rowspan="1" colspan="1">52.2 (9.1)</td><td align="center" rowspan="1" colspan="1">9.1–79.1</td><td align="center" rowspan="1" colspan="1">39.0 (7.6)</td><td align="center" rowspan="1" colspan="1">63.6 (4.1)</td></tr><tr><td align="left" rowspan="1" colspan="1">Proportions of carbohydrates originating from added sugar (%)</td><td align="center" rowspan="1" colspan="1">10.8 (7.4)</td><td align="center" rowspan="1" colspan="1">0–52</td><td align="center" rowspan="1" colspan="1">3.3 (1.3)</td><td align="center" rowspan="1" colspan="1">22.3 (7.6)</td></tr></tbody></table></table-wrap></sec><sec id="S0002-S20002"><title>Clinical examination and collection of saliva samples</title><p>The dental examination has been presented in detail previously (<xref rid="CIT0031" ref-type="bibr">31</xref>). In brief, the oral examination was performed by 3 dental hygienists and 3 dental nurses working in pairs. The hygienists were calibrated intra- and interindividually prior and halfway through the project, with a perfect agreement for periodontal measurements between 60 and 80% and Kappa-coefficients between 0.85 and 0.93 for caries registration. The periodontal examination was performed in 2 randomly selected quadrants, and caries was registered full-mouth. As part of the periodontal examination, gingival margin position (GMP), probing pocket depth (PD), and BOP were recorded in six sites on each tooth. Caries was scored as a clinically manifested lesion present on surface and tooth levels. No information from dental radiographs was included in this study. Stimulated saliva samples were collected by a dental hygienist according to a standardized protocol: the participant was instructed to flush thoroughly and subsequently chew for 1 minute on 1 gram of paraffin gum until a solid bolus was formed. Finally, the participant was instructed to spit repeatedly for 4 minutes, and for the last 3 minutes saliva was collected in a plastic cup, transferred by pipette to a plastic tube, and stored at −80°C until further analysis.</p></sec><sec id="S0002-S20003"><title>DNA isolation and microarray analysis</title><p>DNA isolation from saliva samples was performed according to the manufacturer's guidelines (Roche, Mannheim, Germany). In brief, 200 µl of sample material was thoroughly vortexed, supplemented with 180 µl MagNA Pure Bacterial Lysis buffer (Roche) and 20 µl Proteinase K 20 mg/ml (Roche), and incubated for 1 hour at 37°C. Purification was performed in a Roche MagNA Pure 96 automated DNA extractor, using MagNA Pure 96 DNA and viral NA Small Volume kit (Roche) by specifications according to protocol Pathogen_Universal_200. The final elution volume was 50 µl. HOMIM analyses were performed as described (<xref rid="CIT0006" ref-type="bibr">6</xref>). In brief, HOMIM is a molecular technique using 2 separate polymerase chain reaction (PCR) amplifications and subsequent DNA–DNA hybridization for identification of around 300 bacterial species, as single-stranded fluorescent labeled PCR products are captured by highly specific bacterial 16S rRNA probes (18–20 bases) printed on a customized aldehyde-coated glass slide. In conjunction with studies conducted on saliva samples from DANHES, all probes were thoroughly validated. Probes with cross-reactions and/or missing reactions were excluded from statistical analysis. Further analysis and generation of microbial profiles from scanned microarrays were performed with the HOMIM online tool for analysis (<ext-link ext-link-type="uri" xlink:href="http://bioinformatics.forsyth.org/homim/">http://bioinformatics.forsyth.org/homim/</ext-link>).</p></sec><sec id="S0002-S20004"><title>Collection of data</title><p>The data used in this study were collected through a medical examination, performed by accredited medical personal, and a self-reported questionnaire. The medical examination and the questionnaire gathered information about the physical status and lifestyle-related factors of the participants. Age was registered in whole years at the time point of questionnaire answering. Height was recorded in centimeters (cm), and weight was recorded in kilograms (kg). BMI was calculated using the formula [height (cm)//weight (kg)*weight (kg)]. Smoking status was recorded by questionnaire, and the probands were grouped in current smokers and non-smokers. Information about alcohol consumption was gathered from the questionnaire and recorded as intake of units/week. Dietary data were collected using an Internet-based, 267 items food frequency questionnaire (FFQ), that included 32 photoseries of food portions to quantify portion sizes. The amount of daily energy originating from fat, protein, and carbohydrates was calculated using the Foodcalc program. In addition, the proportion of carbohydrates consisting of added sugar was determined. Information concerning the socioeconomic status of the 12 municipalities was gathered by information from Denmark's statistics using the parameters: average income per year (2007), percentage of unemployed inhabitants, and percentage of inhabitants with a non-western citizenship. In addition, average level of education (International Standard Classification of Education, levels 1–6) was calculated using individual educational information gathered from the questionnaire. In each of the four categories, the municipalities were scored from 1 to 12 (1 being the best ranked municipality) and the values were added (theoretical range 4–48).</p></sec><sec id="S0002-S20005"><title>Formation of subgroups for analysis</title><p>The influence of each of the following variables on the bacterial profile of saliva was tested separately: age, gender, BMI, smoking status, alcohol consumption, proportion of energy originating from fat, proportion of energy origination from protein, and proportion of energy originating from carbohydrates and proportion of carbohydrates originating from added sugar. Based on each of the variables, the total cohort of 292 was divided into quintiles of 58 individuals, and the top quintile was compared to the bottom quintile. In addition, the cohort was split into two subgroups according to gender, establishing a subgroup of males (<italic>n</italic>=126) and females (<italic>n</italic>=166). Each gender subgroup was split into quintiles (males: <italic>n</italic>=25, females: <italic>n</italic>=33), and based on the same parameters, the top quintile was compared to the bottom quintile separately by gender. The subgroup of smokers (<italic>n</italic>=21) was compared to the subgroup of non-smokers (<italic>n</italic>=271). The 12 municipalities were ranked socioeconomically from 1 to 12 based on each of the 4 socioeconomic parameters and the values added up. A total of 133 participants from 5 municipalities constituted a subgroup of high socioeconomic status (16–19 points) that was compared to a subgroup of 159 participants from 7 municipalities with low socioeconomic status (26–44 points) (<xref ref-type="fig" rid="F0001">Fig. 1</xref>).</p></sec><sec id="S0002-S20006"><title>Statistical analysis</title><p>Differences in hybridization patterns were analyzed using the Mann–Whitney test, and differences in data between groups were compared with unpaired <italic>t</italic>-test. For these analyses, a <italic>p-</italic>value <0.05 was considered statistically significant. Comparison of quintiles using frequency and levels (mean HOMIM-value) at taxon/cluster level as parameters of investigation was carried out using a Mann–Whitney test with Benjamini–Hochberg's correction for multiple comparisons (<xref rid="CIT0032" ref-type="bibr">32</xref>). For these analyses, an adjusted <italic>p-</italic>value <0.01 was considered statistically significant. Principal component analysis was used for visualization through data reduction of patterns in this <italic>n</italic>-dimensional dataset. Graph Pad prism 5 (San Diego, CA) and MeV 4.8.1 (<xref rid="CIT0033" ref-type="bibr">33</xref>) were used as statistical software.</p></sec></sec><sec sec-type="results" id="S0003"><title>Results</title><sec id="S0003-S20001"><title>Population characteristics</title><p>Population characteristics are presented in <xref ref-type="table" rid="T0001">Table 1</xref>. For each parameter presented in <xref ref-type="table" rid="T0001">Table 1</xref>, the highest and the lowest quintile were highly significantly different (<italic>p</italic><0.0001).</p></sec><sec id="S0003-S20002"><title>General findings</title><p>In 292 samples from participants with low levels of oral diseases, we observed positive identification for targets of 131 probes. The number of hits per sample was 24.61±9.3 (mean±SD). Sixty-six percent of the targets identified were present in >1% of the samples, while 42% were identified in >5% of the samples. A total of 6 bacterial phyla were identified (<italic>Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Fusobacteria</italic>, and TM7). <italic>Firmicutes</italic> was the predominant phylum present, representing approximately 50% of the total probe signal, followed by <italic>Proteobacteria</italic> and <italic>Bacteroidetes</italic>, each of which accounted for approximately 17% of the targets present. No statistical differences were found in number of targets identified, mean probes per sample, or phylogenetic distribution between subgroups divided on the basis of the parameters investigated (data not shown). A complete list of targets identified is presented in the <ext-link ext-link-type="uri" xlink:href="http://www.journaloforalmicrobiology.net/index.php/jom/rt/suppFiles/23609/0">Supplementary file</ext-link>. The predominant bacterial profile (taxa/clusters identified in >50% of the samples) was dominated by taxa/clusters traditionally associated with oral health representing the genera <italic>Streptococcus</italic> and <italic>Veillonella</italic>. In general, the targets most frequently identified were also those present at the highest levels (mean HOMIM-value). Putative periodontal pathogens such as <italic>Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans</italic>, and <italic>Prevotella intermedia</italic> and suggested cariogenic bacteria such as <italic>Streptococcus mutans</italic> and <italic>Lactobacillus</italic> species were all found in <4% of the samples.</p></sec><sec id="S0003-S20003"><title>Differences between subgroups at bacterial taxon/cluster level</title><p>By comparison of the parameters, that is, age, gender, BMI, alcohol consumption, proportion of energy originating from fat, proportion of energy origination from protein, and proportion of energy originating from carbohydrates and proportion of carbohydrates originating from added sugar separately, no statistical difference in presence or levels (mean HOMIM-value) of any taxon/cluster was observed (adjusted <italic>p-</italic>values>0.01) between the top and bottom quintile of the cohort. Based on the same parameters, no statistical difference in presence or levels of any taxon/cluster was observed when analyzing each gender separately by comparison of the top and bottom quintile of each gender subgroup (adjusted <italic>p-</italic>values>0.01).</p><p>Comparison of smokers (<italic>n</italic>=21) with non-smokers (<italic>n</italic>=271) revealed that 2 bacterial taxa (<italic>Streptococcus sobrinus</italic> and <italic>Eubacterium</italic>[11][G-3] <italic>brachy</italic>) were identified at higher levels in smokers than in non-smokers (adjusted <italic>p-</italic>value<0.01). Principal component analysis displayed clustering of the 21 samples from smokers combined with a random distribution of the samples from non-smokers (<xref ref-type="fig" rid="F0002">Fig. 2</xref>). In addition current smokers (<italic>n</italic>=21) and never-smokers (<italic>n</italic>=148) were compared, and the same bacterial taxa (<italic>S. sobrinus</italic> and <italic>Eubacterium</italic>[11][G-3] <italic>brachy</italic>) were found to be associated with current smokers, when compared to never-smokers. Finally, former-smokers (<italic>n</italic>=123) were compared against never-smokers, and no statistical differences were observed between these groups (data not shown).</p><fig id="F0002" position="float"><label>Fig. 2</label><caption><p>Principal component analysis of smoking status. Smokers (blue, <italic>n</italic>=21) and non-smokers (red, <italic>n</italic>=271) displayed 2-dimensionally with principal component analysis by the 2 principal components, accounting for 30.8% of the variation of the dataset using mean HOMIM-value (level), as parameter for investigation.</p></caption><graphic xlink:href="JOM-6-23609-g002"></graphic></fig><p>When comparing the high socioeconomic status subgroup to the low socioeconomic status subgroup, highly significant differences were observed, as 20 bacterial probes were present with a different frequency between subgroups. Seventeen probes (9 recognizing a bacterial taxon and 8 recognizing a bacterial cluster) were associated with the high socioeconomic status subgroup, and three (1 identifying a bacterial taxon and 2 identifying a bacterial cluster) were associated with the low socioeconomic subgroup. In addition, 25 bacterial probes were identified at different levels in the 2 subgroups. Twenty-two probes (10 recognizing a bacterial taxon and 12 recognizing a bacterial cluster) were more associated with the high socioeconomic status subgroup, and 3 (1 identifying a bacterial taxon and 2 identifying a bacterial cluster) were associated with the low socioeconomic status subgroup. A total of 19 out of 20 taxa/clusters identified with statistically different frequency between the high and low socioeconomic status subgroup were also identified at statistically different levels (<xref ref-type="fig" rid="F0003">Fig. 3</xref>A and <xref ref-type="fig" rid="F0003">3</xref>B). As can be observed from <xref ref-type="fig" rid="F0004">Fig. 4</xref>, a marked difference in the distribution of samples from the high and low socioeconomic status group was identified using principal component analysis, based on the most decisive variable, representing 20% of the mathematical variation in the dataset.</p><fig id="F0003" position="float"><label>Fig. 3</label><caption><p>Differences at taxon/cluster level by socioeconomic status. A. The 20 statistically significant probes are presented based on presence (%) of total amount of samples in the high and low socioeconomic status subgroup, respectively. Black bars: low socioeconomic status subgroup. White bars: high socioeconomic status subgroup. *Adjusted <italic>p-</italic>value < 0.01, *adjusted <italic>p-</italic>value < 0.001, ***adjusted <italic>p-</italic>value < 1.0×10<sup>−4</sup>, ****adjusted <italic>p-</italic>value < 1.0×10<sup>−6</sup>. B. Levels (mean HOMIM-value) of the 25 statistically significant probes are visualized based on means of HOMIM-value in samples from the high and low socioeconomic status subgroup. Black bars: low socioeconomic status subgroup. White bars: high socioeconomic status subgroup. *Adjusted <italic>p-</italic>value < 0.01, **adjusted <italic>p-</italic>value < 0.001, ***adjusted <italic>p-</italic>value < 1.0×10<sup>−5</sup>, ****adjusted <italic>p-</italic>value < 1.0×10<sup>−12</sup>.
</p></caption><graphic xlink:href="JOM-6-23609-g003"></graphic></fig><fig id="F0004" position="float"><label>Fig. 4</label><caption><p>Principal component analysis of socioeconomic status. The subgroup with high socioeconomic status (blue, <italic>n</italic>=133) and the subgroup with low socioeconomic status (red, <italic>n</italic>=159) visualized 2-dimensionally by principal component analysis, with axes expressing the 2 most crucial components accounting for 30.8% of the total variation of the dataset.
</p></caption><graphic xlink:href="JOM-6-23609-g004"></graphic></fig></sec></sec><sec sec-type="discussion" id="S0004"><title>Discussion</title><p>The purpose of this study was to examine whether the bacterial saliva profiles in individuals with low levels of oral diseases were associated with differences in diet intake, lifestyle, or socioeconomic status. By comparison of high-throughput data collected using the HOMIM with available data from the DANHES, we were able to show that the bacterial saliva profile associated with low levels of oral diseases was independent of diet intake, but influenced by smoking and socioeconomic status. To the best of our knowledge, this is the first study to combine high-throughput microbiologic information with comprehensive data, characterizing the composition of the bacterial profile of saliva in individuals with low levels of oral diseases.</p><p>The bacterial profile described in this study was, in spite of high diversity, largely dominated by the phylum of <italic>Firmicutes</italic>, expressed by a massive probe signal representing <italic>Streptococcus</italic> and <italic>Veillonella</italic> species. These findings are in line with studies using 16S rRNA pyrosequencing of saliva samples showing that the microbial profile of saliva in oral health resembles the profiles found on the tongue, the tonsils, and the throat and is characterized by high numbers of <italic>Firmicutes</italic> (<xref rid="CIT0025" ref-type="bibr">25</xref>–<xref rid="CIT0027" ref-type="bibr">27</xref>). Using molecular methods, putative periodontal pathogens such as <italic>P. gingivalis</italic> and <italic>A. actinomycetemcomitans</italic> have been identified to be associated with saliva samples from patients with periodontitis (<xref rid="CIT0034" ref-type="bibr">34</xref>) and <italic>S. mutans</italic> with samples from carious lesions (<xref rid="CIT0035" ref-type="bibr">35</xref>). Interestingly, these bacteria were only found sporadically in this cohort, indicating that saliva is not a major reservoir of putative oral pathogens in individuals with low levels of dental caries and periodontitis.</p><p>One possible limitation of this study was that samples were not collected at the same time of the day. As studies have shown that the oral microbiome might vary considerably over time because of food consumption and mental and physical stress (<xref rid="CIT0036" ref-type="bibr">36</xref>), it cannot be excluded that some of the differences observed might result from diurnal variation in sample collection. In addition, it is important to emphasize that the microarray technique used only provides information of bacterial taxa/clusters with a corresponding probe present on the microarray. Therefore, only parts of the complex saliva bacterial profile were visualized using this technique. Moreover, with a probe length of 18–20 bases, it is not possible to detect all bacteria at species level. As a consequence, some taxa are targeted using cluster probes recognizing 2 or more closely related bacterial taxa, which is why some detail may be lost.</p><p>It is highly interesting that the bacterial profile of saliva appears not to be influenced by diet. Based on different carbohydrate fermentation abilities, aciduric and acidogenic oral streptococci, for example, <italic>S. mutans</italic>, are believed to play an essential role in caries development (<xref rid="CIT0037" ref-type="bibr">37</xref>). However, according to our data, the amount of carbohydrate intake had no influence on the presence of such bacteria in saliva. As frequency of sugar intake is an important risk factor for caries development (<xref rid="CIT0011" ref-type="bibr">11</xref>), it is however possible that the bacterial profile of saliva might be influenced by frequency of sugar intake rather than the amount of sugar consumed, but no information about frequency of sugar intake was included in this study. High levels of BMI have been described to be associated with high occurrence of caries lesions (<xref rid="CIT0008" ref-type="bibr">8</xref>), and intake of high amounts of alcohol has been suggested as a risk factor for periodontitis (<xref rid="CIT0014" ref-type="bibr">14</xref>), as well as protective against periodontitis (<xref rid="CIT0013" ref-type="bibr">13</xref>). In the population examined in this study, these parameters had no influence on the bacterial saliva profile, even when the upper and lower quintiles were compared. These findings suggest that the bacterial profile of saliva is, in general, not associated with the mechanism linking different lifestyle factors with major oral diseases. However, the value of these results might be limited by a problem often encountered in epidemiological research: individuals participating in medical studies tend to be healthier than the general population, as visualized by the small percentage of current smokers in this group (7.2%), compared to the background Danish population (22%). This may limit the variability, and hence the chance to find significant associations. This may also compromise the generalizability of the conclusions drawn to general population subsets. Furthermore, in this study quintiles were chosen for comparison of the most extreme ends of the cohort based on each parameter investigated. The advantage with this approach was accomplishment of major differences between the subgroups analyzed. However, as this strategy resulted in relatively small subgroups, there was risk of loss of statistical power, theoretically masking some of the associations investigated.</p><p>In this study, the bacterial taxa <italic>S. sobrinus</italic> and <italic>Eubacterium</italic>[11][G-3] <italic>brachy</italic> were found to be associated with saliva samples from smokers when compared to a group of non-smokers and never-smokers, respectively. As discussed in a recent review of the literature (<xref rid="CIT0038" ref-type="bibr">38</xref>), a possible association between smoking and alterations of the subgingival bacterial profile remains controversial. Thus, even though our data suggest that smoking is associated with a different bacterial profile of saliva than the one observed in non-smokers, this needs to be addressed in future studies.</p><p>Our results suggest that the bacterial profile of saliva is influenced by socioeconomic status. Thus, when dividing the cohort into subgroups of high and low socioeconomic status, 20 and 25 probes differed significantly between subgroups based on presence and levels (mean HOMIM-value), receptively (<xref ref-type="fig" rid="F0003">Fig. 3</xref>A and <xref ref-type="fig" rid="F0003">3</xref>B). As can be observed from <xref ref-type="fig" rid="F0004">Fig. 4</xref>, the 2 subgroups clustered almost completely separately based on the principal component, expressing 20% of the total variation of the dataset. This finding is in accordance with other studies suggesting that differences of the salivary microbiome observed in various geographic locations might be explained by different lifestyle and socioeconomic profiles between populations (<xref rid="CIT0025" ref-type="bibr">25</xref>, <xref rid="CIT0027" ref-type="bibr">27</xref>). It is noteworthy that the differences observed at taxon/cluster level affected probes targeting bacterial taxa as <italic>Veillonella parvula</italic> and <italic>Veillonella atypica</italic> and clusters as <italic>Streptococcus parasanguinis</italic>, all being part of the predominant commensal bacterial profile of saliva. In this study, we used general information about the municipalities from Denmark's statistics concerning the parameters: average income a year, percentage of unemployed inhabitants, and percentage of inhabitants with a non-western citizenship, in combination with average level of education (International Standard Classification of Education, levels 1–6), calculated from individually based information. As the socioeconomic variable was mostly constructed using general municipality based information, there is a risk that this variable was not fully representative of the actual individuals participating in the study, questioning extrapolation of the results obtained.</p><p>In conclusion, with the limitations mentioned above, this study suggests that the bacterial saliva profile in individuals with low levels of oral diseases is not influenced by diet. Conversely, smoking and possibly socioeconomic status seem to affect the bacterial profile of saliva. Prospective studies may reveal if bacterial profiles of saliva can be used as biomarkers of health and disease.</p></sec></body><back><ack><title>Acknowledgements</title><p>The authors wish to thank the National Institute of Public Health, University of Southern Denmark, Copenhagen, Denmark, for conducting the DANHES, Dr. Lisa B. Christensen for her assistance analyzing socioeconomic parameters, Dr. Mogens Kilian for his help interpreting microbiological data, and Sean Cotton, Alexis Kokaras, and Christina Murphy from the Forsyth Institute for laboratory assistance.</p></ack><sec id="S0005"><title>Conflict of interest and funding</title><p>The authors declare no conflicts of interest in this study. 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