EdenteV1, PascalFrancis, Corpus, bibRecord, 000392

Expression of p21 and p53 in rat gingival and human oral epithelial cells after cyclosporine A treatment

Identifieur interne : 000392 ( PascalFrancis/Corpus ); précédent : 000391; suivant : 000393

Expression of p21 and p53 in rat gingival and human oral epithelial cells after cyclosporine A treatment

Auteurs : H.-P. Tu ; E. Fu ; Y.-T. Chen ; M.-H. Wu ; L.-C. Cheng ; S.-F. Yang

Source :

Journal of periodontal research [ 0022-3484 ] ; 2008.

RBID : Pascal:08-0099424

Descripteurs français

Pascal (Inist)
- Ciclosporine, Traitement, Gène CDKN1A, Gène TP53, Gène suppresseur tumeur, Animal, Rat, Gencive, Homme, Voie orale, Cellule épithéliale, Immunodépresseur, Stomatologie.

English descriptors

KwdEn :
- Animal, CDKN1A Gene, Ciclosporin, Epithelial cell, Gingiva, Human, Immunosuppressive agent, Oral administration, Rat, Stomatology, TP53 Gene, Treatment, Tumor suppressor gene.

Abstract

Background and Objective: Expression of p21 and p53 were examined, at gene and protein levels, in edentulous gingival epithelial cells from rats and from a human oral epidermoid carcinoma cell line, OECM1, after cyclosporine A therapy. Material and Methods: In vivo: 20 partially edentulous SD rats were assigned into cyclosporine A feeding and control groups. After the rats were killed, p21 and p53 in gingiva were evaluated by reverse transcription-polymerase chain reaction and immunohistochemistry. In vitro: after cyclosporine A treatment, p21 and p53 of OECM1 cells were evaluated by western blot and the luciferase assay. The distribution of OECM 1 cells in each phase of the cell cycle was evaluated by flow cytometry. Results: The mRNA expression of p21 was significantly higher in the cyclosporine A group than in the control group. A greater number of positive anti-p21-stained cells were observed in the gingival epithelium of the cyclosporine A group than in the control group. Significantly higher levels of p21 protein and activity were observed in OECM1 cells after cyclosporine A treatment than in cells without treatment. A relative increase of cells in G0/G1 phases, and a decrease of cells in G2/M phases, were observed in OECM1 cells after cyclosporine A treatment. Conclusion: In the present study, higher p21 mRNA and protein expressions were observed after cyclosporine A treatment. Thus, an up-regulation of p21 expression, via a p53-independent pathway, by cyclosporine A in gingival and oral epithelial cells was suggested.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0022-3484`
A03		`1`		`@0 J. periodontal res.`
A05				`@2 43`
A06				`@2 1`
A08	`01`	`1`	`ENG`	`@1 Expression of p21 and p53 in rat gingival and human oral epithelial cells after cyclosporine A treatment`
A11	`01`	`1`		`@1 TU (H.-P.)`
A11	`02`	`1`		`@1 FU (E.)`
A11	`03`	`1`		`@1 CHEN (Y.-T.)`
A11	`04`	`1`		`@1 WU (M.-H.)`
A11	`05`	`1`		`@1 CHENG (L.-C.)`
A11	`06`	`1`		`@1 YANG (S.-F.)`
A14	`01`			`@1 Institute of Oral Biology, National Yang-Ming University @2 Taipei @3 TWN @Z 1 aut.`
A14	`02`			`@1 Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital @2 Taipei @3 TWN @Z 2 aut. @Z 3 aut.`
A14	`03`			`@1 Digitalgene Biosciences Co., Ltd @2 Taipei @3 TWN @Z 3 aut.`
A14	`04`			`@1 Graduate Institute of Life Sciences, National Defense Medical Center @2 Taipei @3 TWN @Z 4 aut.`
A14	`05`			`@1 Department of Surgery, Chang Gung Memorial Hospital @2 Taoyuan @3 TWN @Z 5 aut.`
A14	`06`			`@1 School of Dentistry, National Yang-Ming University & West Garden Hospital @2 Taipei @3 TWN @Z 6 aut.`
A20				`@1 32-39`
A21				`@1 2008`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 15072 @5 354000183417690050`
A44				`@0 0000 @1 © 2008 INIST-CNRS. All rights reserved.`
A45				`@0 66 ref.`
A47	`01`	`1`		`@0 08-0099424`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Journal of periodontal research`
A66	`01`			`@0 GBR`
C01	`01`		`ENG`	@0 Background and Objective: Expression of p21 and p53 were examined, at gene and protein levels, in edentulous gingival epithelial cells from rats and from a human oral epidermoid carcinoma cell line, OECM1, after cyclosporine A therapy. Material and Methods: In vivo: 20 partially edentulous SD rats were assigned into cyclosporine A feeding and control groups. After the rats were killed, p21 and p53 in gingiva were evaluated by reverse transcription-polymerase chain reaction and immunohistochemistry. In vitro: after cyclosporine A treatment, p21 and p53 of OECM1 cells were evaluated by western blot and the luciferase assay. The distribution of OECM 1 cells in each phase of the cell cycle was evaluated by flow cytometry. Results: The mRNA expression of p21 was significantly higher in the cyclosporine A group than in the control group. A greater number of positive anti-p21-stained cells were observed in the gingival epithelium of the cyclosporine A group than in the control group. Significantly higher levels of p21 protein and activity were observed in OECM1 cells after cyclosporine A treatment than in cells without treatment. A relative increase of cells in G0/G1 phases, and a decrease of cells in G2/M phases, were observed in OECM1 cells after cyclosporine A treatment. Conclusion: In the present study, higher p21 mRNA and protein expressions were observed after cyclosporine A treatment. Thus, an up-regulation of p21 expression, via a p53-independent pathway, by cyclosporine A in gingival and oral epithelial cells was suggested.
C02	`01`	`X`		`@0 002B10`
C03	`01`	`X`	`FRE`	`@0 Ciclosporine @2 NK @2 FR @5 04`
C03	`01`	`X`	`ENG`	`@0 Ciclosporin @2 NK @2 FR @5 04`
C03	`01`	`X`	`SPA`	`@0 Ciclosporina @2 NK @2 FR @5 04`
C03	`02`	`X`	`FRE`	`@0 Traitement @5 05`
C03	`02`	`X`	`ENG`	`@0 Treatment @5 05`
C03	`02`	`X`	`SPA`	`@0 Tratamiento @5 05`
C03	`03`	`X`	`FRE`	`@0 Gène CDKN1A @5 07`
C03	`03`	`X`	`ENG`	`@0 CDKN1A Gene @5 07`
C03	`03`	`X`	`SPA`	`@0 Gen CDKN1A @5 07`
C03	`04`	`X`	`FRE`	`@0 Gène TP53 @5 08`
C03	`04`	`X`	`ENG`	`@0 TP53 Gene @5 08`
C03	`04`	`X`	`SPA`	`@0 Gen TP53 @5 08`
C03	`05`	`X`	`FRE`	`@0 Gène suppresseur tumeur @5 09`
C03	`05`	`X`	`ENG`	`@0 Tumor suppressor gene @5 09`
C03	`05`	`X`	`SPA`	`@0 Gen supresor tumor @5 09`
C03	`06`	`X`	`FRE`	`@0 Animal @5 13`
C03	`06`	`X`	`ENG`	`@0 Animal @5 13`
C03	`06`	`X`	`SPA`	`@0 Animal @5 13`
C03	`07`	`X`	`FRE`	`@0 Rat @5 14`
C03	`07`	`X`	`ENG`	`@0 Rat @5 14`
C03	`07`	`X`	`SPA`	`@0 Rata @5 14`
C03	`08`	`X`	`FRE`	`@0 Gencive @5 15`
C03	`08`	`X`	`ENG`	`@0 Gingiva @5 15`
C03	`08`	`X`	`SPA`	`@0 Encía @5 15`
C03	`09`	`X`	`FRE`	`@0 Homme @5 16`
C03	`09`	`X`	`ENG`	`@0 Human @5 16`
C03	`09`	`X`	`SPA`	`@0 Hombre @5 16`
C03	`10`	`X`	`FRE`	`@0 Voie orale @5 17`
C03	`10`	`X`	`ENG`	`@0 Oral administration @5 17`
C03	`10`	`X`	`SPA`	`@0 Vía oral @5 17`
C03	`11`	`X`	`FRE`	`@0 Cellule épithéliale @5 18`
C03	`11`	`X`	`ENG`	`@0 Epithelial cell @5 18`
C03	`11`	`X`	`SPA`	`@0 Célula epitelial @5 18`
C03	`12`	`X`	`FRE`	`@0 Immunodépresseur @5 19`
C03	`12`	`X`	`ENG`	`@0 Immunosuppressive agent @5 19`
C03	`12`	`X`	`SPA`	`@0 Inmunodepresor @5 19`
C03	`13`	`X`	`FRE`	`@0 Stomatologie @5 20`
C03	`13`	`X`	`ENG`	`@0 Stomatology @5 20`
C03	`13`	`X`	`SPA`	`@0 Estomatología @5 20`
C07	`01`	`X`	`FRE`	`@0 Rodentia @2 NS`
C07	`01`	`X`	`ENG`	`@0 Rodentia @2 NS`
C07	`01`	`X`	`SPA`	`@0 Rodentia @2 NS`
C07	`02`	`X`	`FRE`	`@0 Mammalia @2 NS`
C07	`02`	`X`	`ENG`	`@0 Mammalia @2 NS`
C07	`02`	`X`	`SPA`	`@0 Mammalia @2 NS`
C07	`03`	`X`	`FRE`	`@0 Vertebrata @2 NS`
C07	`03`	`X`	`ENG`	`@0 Vertebrata @2 NS`
C07	`03`	`X`	`SPA`	`@0 Vertebrata @2 NS`
C07	`04`	`X`	`FRE`	`@0 Inhibiteur de la calcineurine @4 INC @5 86`
N21				`@1 052`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 08-0099424 INIST
ET :	Expression of p21 and p53 in rat gingival and human oral epithelial cells after cyclosporine A treatment
AU :	TU (H.-P.); FU (E.); CHEN (Y.-T.); WU (M.-H.); CHENG (L.-C.); YANG (S.-F.)
AF :	Institute of Oral Biology, National Yang-Ming University/Taipei/Taïwan (1 aut.); Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital/Taipei/Taïwan (2 aut., 3 aut.); Digitalgene Biosciences Co., Ltd/Taipei/Taïwan (3 aut.); Graduate Institute of Life Sciences, National Defense Medical Center/Taipei/Taïwan (4 aut.); Department of Surgery, Chang Gung Memorial Hospital/Taoyuan/Taïwan (5 aut.); School of Dentistry, National Yang-Ming University & West Garden Hospital/Taipei/Taïwan (6 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of periodontal research; ISSN 0022-3484; Royaume-Uni; Da. 2008; Vol. 43; No. 1; Pp. 32-39; Bibl. 66 ref.
LA :	Anglais
EA :	Background and Objective: Expression of p21 and p53 were examined, at gene and protein levels, in edentulous gingival epithelial cells from rats and from a human oral epidermoid carcinoma cell line, OECM1, after cyclosporine A therapy. Material and Methods: In vivo: 20 partially edentulous SD rats were assigned into cyclosporine A feeding and control groups. After the rats were killed, p21 and p53 in gingiva were evaluated by reverse transcription-polymerase chain reaction and immunohistochemistry. In vitro: after cyclosporine A treatment, p21 and p53 of OECM1 cells were evaluated by western blot and the luciferase assay. The distribution of OECM 1 cells in each phase of the cell cycle was evaluated by flow cytometry. Results: The mRNA expression of p21 was significantly higher in the cyclosporine A group than in the control group. A greater number of positive anti-p21-stained cells were observed in the gingival epithelium of the cyclosporine A group than in the control group. Significantly higher levels of p21 protein and activity were observed in OECM1 cells after cyclosporine A treatment than in cells without treatment. A relative increase of cells in G0/G1 phases, and a decrease of cells in G2/M phases, were observed in OECM1 cells after cyclosporine A treatment. Conclusion: In the present study, higher p21 mRNA and protein expressions were observed after cyclosporine A treatment. Thus, an up-regulation of p21 expression, via a p53-independent pathway, by cyclosporine A in gingival and oral epithelial cells was suggested.
CC :	002B10
FD :	Ciclosporine; Traitement; Gène CDKN1A; Gène TP53; Gène suppresseur tumeur; Animal; Rat; Gencive; Homme; Voie orale; Cellule épithéliale; Immunodépresseur; Stomatologie
FG :	Rodentia; Mammalia; Vertebrata; Inhibiteur de la calcineurine
ED :	Ciclosporin; Treatment; CDKN1A Gene; TP53 Gene; Tumor suppressor gene; Animal; Rat; Gingiva; Human; Oral administration; Epithelial cell; Immunosuppressive agent; Stomatology
EG :	Rodentia; Mammalia; Vertebrata
SD :	Ciclosporina; Tratamiento; Gen CDKN1A; Gen TP53; Gen supresor tumor; Animal; Rata; Encía; Hombre; Vía oral; Célula epitelial; Inmunodepresor; Estomatología
LO :	INIST-15072.354000183417690050
ID :	08-0099424

Links to Exploration step

Pascal:08-0099424

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Expression of p21 and p53 in rat gingival and human oral epithelial cells after cyclosporine A treatment</title>
<author><name sortKey="Tu, H P" sort="Tu, H P" uniqKey="Tu H" first="H.-P." last="Tu">H.-P. Tu</name>
<affiliation><inist:fA14 i1="01"><s1>Institute of Oral Biology, National Yang-Ming University</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Fu, E" sort="Fu, E" uniqKey="Fu E" first="E." last="Fu">E. Fu</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Chen, Y T" sort="Chen, Y T" uniqKey="Chen Y" first="Y.-T." last="Chen">Y.-T. Chen</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Digitalgene Biosciences Co., Ltd</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Wu, M H" sort="Wu, M H" uniqKey="Wu M" first="M.-H." last="Wu">M.-H. Wu</name>
<affiliation><inist:fA14 i1="04"><s1>Graduate Institute of Life Sciences, National Defense Medical Center</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Cheng, L C" sort="Cheng, L C" uniqKey="Cheng L" first="L.-C." last="Cheng">L.-C. Cheng</name>
<affiliation><inist:fA14 i1="05"><s1>Department of Surgery, Chang Gung Memorial Hospital</s1>
<s2>Taoyuan</s2>
<s3>TWN</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Yang, S F" sort="Yang, S F" uniqKey="Yang S" first="S.-F." last="Yang">S.-F. Yang</name>
<affiliation><inist:fA14 i1="06"><s1>School of Dentistry, National Yang-Ming University & West Garden Hospital</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">08-0099424</idno>
<date when="2008">2008</date>
<idno type="stanalyst">PASCAL 08-0099424 INIST</idno>
<idno type="RBID">Pascal:08-0099424</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000392</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Expression of p21 and p53 in rat gingival and human oral epithelial cells after cyclosporine A treatment</title>
<author><name sortKey="Tu, H P" sort="Tu, H P" uniqKey="Tu H" first="H.-P." last="Tu">H.-P. Tu</name>
<affiliation><inist:fA14 i1="01"><s1>Institute of Oral Biology, National Yang-Ming University</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Fu, E" sort="Fu, E" uniqKey="Fu E" first="E." last="Fu">E. Fu</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Chen, Y T" sort="Chen, Y T" uniqKey="Chen Y" first="Y.-T." last="Chen">Y.-T. Chen</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</inist:fA14>
</affiliation>
<affiliation><inist:fA14 i1="03"><s1>Digitalgene Biosciences Co., Ltd</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Wu, M H" sort="Wu, M H" uniqKey="Wu M" first="M.-H." last="Wu">M.-H. Wu</name>
<affiliation><inist:fA14 i1="04"><s1>Graduate Institute of Life Sciences, National Defense Medical Center</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Cheng, L C" sort="Cheng, L C" uniqKey="Cheng L" first="L.-C." last="Cheng">L.-C. Cheng</name>
<affiliation><inist:fA14 i1="05"><s1>Department of Surgery, Chang Gung Memorial Hospital</s1>
<s2>Taoyuan</s2>
<s3>TWN</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Yang, S F" sort="Yang, S F" uniqKey="Yang S" first="S.-F." last="Yang">S.-F. Yang</name>
<affiliation><inist:fA14 i1="06"><s1>School of Dentistry, National Yang-Ming University & West Garden Hospital</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of periodontal research</title>
<title level="j" type="abbreviated">J. periodontal res.</title>
<idno type="ISSN">0022-3484</idno>
<imprint><date when="2008">2008</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of periodontal research</title>
<title level="j" type="abbreviated">J. periodontal res.</title>
<idno type="ISSN">0022-3484</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animal</term>
<term>CDKN1A Gene</term>
<term>Ciclosporin</term>
<term>Epithelial cell</term>
<term>Gingiva</term>
<term>Human</term>
<term>Immunosuppressive agent</term>
<term>Oral administration</term>
<term>Rat</term>
<term>Stomatology</term>
<term>TP53 Gene</term>
<term>Treatment</term>
<term>Tumor suppressor gene</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Ciclosporine</term>
<term>Traitement</term>
<term>Gène CDKN1A</term>
<term>Gène TP53</term>
<term>Gène suppresseur tumeur</term>
<term>Animal</term>
<term>Rat</term>
<term>Gencive</term>
<term>Homme</term>
<term>Voie orale</term>
<term>Cellule épithéliale</term>
<term>Immunodépresseur</term>
<term>Stomatologie</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Background and Objective: Expression of p21 and p53 were examined, at gene and protein levels, in edentulous gingival epithelial cells from rats and from a human oral epidermoid carcinoma cell line, OECM1, after cyclosporine A therapy. Material and Methods: In vivo: 20 partially edentulous SD rats were assigned into cyclosporine A feeding and control groups. After the rats were killed, p21 and p53 in gingiva were evaluated by reverse transcription-polymerase chain reaction and immunohistochemistry. In vitro: after cyclosporine A treatment, p21 and p53 of OECM1 cells were evaluated by western blot and the luciferase assay. The distribution of OECM 1 cells in each phase of the cell cycle was evaluated by flow cytometry. Results: The mRNA expression of p21 was significantly higher in the cyclosporine A group than in the control group. A greater number of positive anti-p21-stained cells were observed in the gingival epithelium of the cyclosporine A group than in the control group. Significantly higher levels of p21 protein and activity were observed in OECM1 cells after cyclosporine A treatment than in cells without treatment. A relative increase of cells in G0/G1 phases, and a decrease of cells in G2/M phases, were observed in OECM1 cells after cyclosporine A treatment. Conclusion: In the present study, higher p21 mRNA and protein expressions were observed after cyclosporine A treatment. Thus, an up-regulation of p21 expression, via a p53-independent pathway, by cyclosporine A in gingival and oral epithelial cells was suggested.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0022-3484</s0>
</fA01>
<fA03 i2="1"><s0>J. periodontal res.</s0>
</fA03>
<fA05><s2>43</s2>
</fA05>
<fA06><s2>1</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Expression of p21 and p53 in rat gingival and human oral epithelial cells after cyclosporine A treatment</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>TU (H.-P.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>FU (E.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>CHEN (Y.-T.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>WU (M.-H.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>CHENG (L.-C.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>YANG (S.-F.)</s1>
</fA11>
<fA14 i1="01"><s1>Institute of Oral Biology, National Yang-Ming University</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>1 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Digitalgene Biosciences Co., Ltd</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Graduate Institute of Life Sciences, National Defense Medical Center</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Department of Surgery, Chang Gung Memorial Hospital</s1>
<s2>Taoyuan</s2>
<s3>TWN</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="06"><s1>School of Dentistry, National Yang-Ming University & West Garden Hospital</s1>
<s2>Taipei</s2>
<s3>TWN</s3>
<sZ>6 aut.</sZ>
</fA14>
<fA20><s1>32-39</s1>
</fA20>
<fA21><s1>2008</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>15072</s2>
<s5>354000183417690050</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2008 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>66 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>08-0099424</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Journal of periodontal research</s0>
</fA64>
<fA66 i1="01"><s0>GBR</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Background and Objective: Expression of p21 and p53 were examined, at gene and protein levels, in edentulous gingival epithelial cells from rats and from a human oral epidermoid carcinoma cell line, OECM1, after cyclosporine A therapy. Material and Methods: In vivo: 20 partially edentulous SD rats were assigned into cyclosporine A feeding and control groups. After the rats were killed, p21 and p53 in gingiva were evaluated by reverse transcription-polymerase chain reaction and immunohistochemistry. In vitro: after cyclosporine A treatment, p21 and p53 of OECM1 cells were evaluated by western blot and the luciferase assay. The distribution of OECM 1 cells in each phase of the cell cycle was evaluated by flow cytometry. Results: The mRNA expression of p21 was significantly higher in the cyclosporine A group than in the control group. A greater number of positive anti-p21-stained cells were observed in the gingival epithelium of the cyclosporine A group than in the control group. Significantly higher levels of p21 protein and activity were observed in OECM1 cells after cyclosporine A treatment than in cells without treatment. A relative increase of cells in G0/G1 phases, and a decrease of cells in G2/M phases, were observed in OECM1 cells after cyclosporine A treatment. Conclusion: In the present study, higher p21 mRNA and protein expressions were observed after cyclosporine A treatment. Thus, an up-regulation of p21 expression, via a p53-independent pathway, by cyclosporine A in gingival and oral epithelial cells was suggested.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002B10</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Ciclosporine</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>04</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Ciclosporin</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>04</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Ciclosporina</s0>
<s2>NK</s2>
<s2>FR</s2>
<s5>04</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Traitement</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Treatment</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Tratamiento</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Gène CDKN1A</s0>
<s5>07</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>CDKN1A Gene</s0>
<s5>07</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Gen CDKN1A</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Gène TP53</s0>
<s5>08</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>TP53 Gene</s0>
<s5>08</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Gen TP53</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Gène suppresseur tumeur</s0>
<s5>09</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Tumor suppressor gene</s0>
<s5>09</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Gen supresor tumor</s0>
<s5>09</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Animal</s0>
<s5>13</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Animal</s0>
<s5>13</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Animal</s0>
<s5>13</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Rat</s0>
<s5>14</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Rat</s0>
<s5>14</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Rata</s0>
<s5>14</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Gencive</s0>
<s5>15</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Gingiva</s0>
<s5>15</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Encía</s0>
<s5>15</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Homme</s0>
<s5>16</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Human</s0>
<s5>16</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Hombre</s0>
<s5>16</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Voie orale</s0>
<s5>17</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Oral administration</s0>
<s5>17</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Vía oral</s0>
<s5>17</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Cellule épithéliale</s0>
<s5>18</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Epithelial cell</s0>
<s5>18</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Célula epitelial</s0>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Immunodépresseur</s0>
<s5>19</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Immunosuppressive agent</s0>
<s5>19</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Inmunodepresor</s0>
<s5>19</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Stomatologie</s0>
<s5>20</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Stomatology</s0>
<s5>20</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Estomatología</s0>
<s5>20</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rodentia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Mammalia</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Inhibiteur de la calcineurine</s0>
<s4>INC</s4>
<s5>86</s5>
</fC07>
<fN21><s1>052</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 08-0099424 INIST</NO>
<ET>Expression of p21 and p53 in rat gingival and human oral epithelial cells after cyclosporine A treatment</ET>
<AU>TU (H.-P.); FU (E.); CHEN (Y.-T.); WU (M.-H.); CHENG (L.-C.); YANG (S.-F.)</AU>
<AF>Institute of Oral Biology, National Yang-Ming University/Taipei/Taïwan (1 aut.); Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital/Taipei/Taïwan (2 aut., 3 aut.); Digitalgene Biosciences Co., Ltd/Taipei/Taïwan (3 aut.); Graduate Institute of Life Sciences, National Defense Medical Center/Taipei/Taïwan (4 aut.); Department of Surgery, Chang Gung Memorial Hospital/Taoyuan/Taïwan (5 aut.); School of Dentistry, National Yang-Ming University & West Garden Hospital/Taipei/Taïwan (6 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of periodontal research; ISSN 0022-3484; Royaume-Uni; Da. 2008; Vol. 43; No. 1; Pp. 32-39; Bibl. 66 ref.</SO>
<LA>Anglais</LA>
<EA>Background and Objective: Expression of p21 and p53 were examined, at gene and protein levels, in edentulous gingival epithelial cells from rats and from a human oral epidermoid carcinoma cell line, OECM1, after cyclosporine A therapy. Material and Methods: In vivo: 20 partially edentulous SD rats were assigned into cyclosporine A feeding and control groups. After the rats were killed, p21 and p53 in gingiva were evaluated by reverse transcription-polymerase chain reaction and immunohistochemistry. In vitro: after cyclosporine A treatment, p21 and p53 of OECM1 cells were evaluated by western blot and the luciferase assay. The distribution of OECM 1 cells in each phase of the cell cycle was evaluated by flow cytometry. Results: The mRNA expression of p21 was significantly higher in the cyclosporine A group than in the control group. A greater number of positive anti-p21-stained cells were observed in the gingival epithelium of the cyclosporine A group than in the control group. Significantly higher levels of p21 protein and activity were observed in OECM1 cells after cyclosporine A treatment than in cells without treatment. A relative increase of cells in G0/G1 phases, and a decrease of cells in G2/M phases, were observed in OECM1 cells after cyclosporine A treatment. Conclusion: In the present study, higher p21 mRNA and protein expressions were observed after cyclosporine A treatment. Thus, an up-regulation of p21 expression, via a p53-independent pathway, by cyclosporine A in gingival and oral epithelial cells was suggested.</EA>
<CC>002B10</CC>
<FD>Ciclosporine; Traitement; Gène CDKN1A; Gène TP53; Gène suppresseur tumeur; Animal; Rat; Gencive; Homme; Voie orale; Cellule épithéliale; Immunodépresseur; Stomatologie</FD>
<FG>Rodentia; Mammalia; Vertebrata; Inhibiteur de la calcineurine</FG>
<ED>Ciclosporin; Treatment; CDKN1A Gene; TP53 Gene; Tumor suppressor gene; Animal; Rat; Gingiva; Human; Oral administration; Epithelial cell; Immunosuppressive agent; Stomatology</ED>
<EG>Rodentia; Mammalia; Vertebrata</EG>
<SD>Ciclosporina; Tratamiento; Gen CDKN1A; Gen TP53; Gen supresor tumor; Animal; Rata; Encía; Hombre; Vía oral; Célula epitelial; Inmunodepresor; Estomatología</SD>
<LO>INIST-15072.354000183417690050</LO>
<ID>08-0099424</ID>
</server>
</inist>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV1/Data/PascalFrancis/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000392 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000392 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Santé
   |area=    EdenteV1
   |flux=    PascalFrancis
   |étape=   Corpus
   |type=    RBID
   |clé=     Pascal:08-0099424
   |texte=   Expression of p21 and p53 in rat gingival and human oral epithelial cells after cyclosporine A treatment
}}

This area was generated with Dilib version V0.6.33.
Data generation: Mon Dec 4 11:02:15 2017. Site generation: Tue Sep 29 19:14:38 2020

	Serveur d'exploration sur le patient édenté (maquette)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le patient édenté (maquette)

Expression of p21 and p53 in rat gingival and human oral epithelial cells after cyclosporine A treatment

Expression of p21 and p53 in rat gingival and human oral epithelial cells after cyclosporine A treatment

Source :

Descripteurs français

English descriptors

Abstract

Notice en format standard (ISO 2709)

Format Inist (serveur)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri