Evaluation of biocompatibility and degradation of chitosan nanofiber membrane crosslinked with genipin
Identifieur interne : 000033 ( Istex/Corpus ); précédent : 000032; suivant : 000034Evaluation of biocompatibility and degradation of chitosan nanofiber membrane crosslinked with genipin
Auteurs : Ankit J. Bavariya ; P. Andrew Norowski Jr. ; K. Mark Anderson ; Pradeep C. Adatrow ; Franklin Garcia-Godoy ; Sidney H. Stein ; Joel D. BumgardnerSource :
- Journal of Biomedical Materials Research Part B: Applied Biomaterials [ 1552-4973 ] ; 2014-07.
Abstract
Chitosan, a natural polysaccharide, has demonstrated potential as a degradable biocompatible guided bone regeneration membrane. This study aimed to evaluate the in vivo biocompatibility and degradation of chitosan nanofiber membranes, with and without genipin crosslinking as compared with a commercial collagen membrane in rat model. Chitosan nanofiber membranes, with and without genipin crosslinking, and collagen membrane (control) were implanted subcutaneously in the backs of 30 rats. The membranes were analyzed histologically at 2, 4, 8, 12, 16, and 20 weeks. Sections were viewed and graded by a blinded pathologist using a 4‐point scoring system (0 = absent, 1 = mild, 2 = moderate, and 3 = severe) to determine the tissue reaction to the membranes and to observe membrane degradation. There was no statistically significant difference in histological scores among chitosan and collagen membranes at different time points. Absence or minimal inflammation was observed in 57–74% of the membranes across all groups. Most chitosan membranes persisted for 16–20 weeks, whereas most collagen membranes disappeared by resorption at 12–16 weeks. The general tissue response to chitosan nanofiber membranes with and without genipin crosslinking, was similar to that of control commercial collagen membrane. However, the chitosan membranes exhibited slower degradation rates than collagen membranes. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1084–1092, 2014.
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DOI: 10.1002/jbm.b.33090
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Bavariya</name><affiliation><mods:affiliation>Department of Periodontology, University of Tennessee Health Science Center, College of Dentistry, Tennessee, Memphis</mods:affiliation></affiliation></author><author><name sortKey="Andrew Norowski Jr, P" sort="Andrew Norowski Jr, P" uniqKey="Andrew Norowski Jr P" first="P." last="Andrew Norowski Jr.">P. Andrew Norowski Jr.</name><affiliation><mods:affiliation>Department of Biomedical Engineering, University of Memphis, Herff College of Engineering, Tennessee, Memphis</mods:affiliation></affiliation></author><author><name sortKey="Mark Anderson, K" sort="Mark Anderson, K" uniqKey="Mark Anderson K" first="K." last="Mark Anderson">K. Mark Anderson</name><affiliation><mods:affiliation>Department of Diagnostic Sciences and Oral Medicine, University of Tennessee Health Science Center, College of Dentistry, Tennessee, Memphis</mods:affiliation></affiliation></author><author><name sortKey="Adatrow, Pradeep C" sort="Adatrow, Pradeep C" uniqKey="Adatrow P" first="Pradeep C." last="Adatrow">Pradeep C. Adatrow</name><affiliation><mods:affiliation>Department of Prosthodontics, University of Tennessee Health Science Center, College of Dentistry, Tennessee, Memphis</mods:affiliation></affiliation></author><author><name sortKey="Garcia Odoy, Franklin" sort="Garcia Odoy, Franklin" uniqKey="Garcia Odoy F" first="Franklin" last="Garcia-Godoy">Franklin Garcia-Godoy</name><affiliation><mods:affiliation>Department of Bioscience Research, University of Tennessee Health Science Center, College of Dentistry, Tennessee, Memphis</mods:affiliation></affiliation></author><author><name sortKey="Stein, Sidney H" sort="Stein, Sidney H" uniqKey="Stein S" first="Sidney H." last="Stein">Sidney H. Stein</name><affiliation><mods:affiliation>Department of Periodontology, University of Tennessee Health Science Center, College of Dentistry, Tennessee, Memphis</mods:affiliation></affiliation></author><author><name sortKey="Bumgardner, Joel D" sort="Bumgardner, Joel D" uniqKey="Bumgardner J" first="Joel D." last="Bumgardner">Joel D. Bumgardner</name><affiliation><mods:affiliation>Department of Biomedical Engineering, University of Memphis, Herff College of Engineering, Tennessee, Memphis</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: jbmgrdnr@memphis.edu</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Biomedical Materials Research Part B: Applied Biomaterials</title><title level="j" type="alt">JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B: APPLIED BIOMATERIALS</title><idno type="ISSN">1552-4973</idno><idno type="eISSN">1552-4981</idno><imprint><biblScope unit="vol">102</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="1084">1084</biblScope><biblScope unit="page" to="1092">1092</biblScope><biblScope unit="page-count">9</biblScope><date type="published" when="2014-07">2014-07</date></imprint><idno type="ISSN">1552-4973</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1552-4973</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Chitosan, a natural polysaccharide, has demonstrated potential as a degradable biocompatible guided bone regeneration membrane. This study aimed to evaluate the in vivo biocompatibility and degradation of chitosan nanofiber membranes, with and without genipin crosslinking as compared with a commercial collagen membrane in rat model. Chitosan nanofiber membranes, with and without genipin crosslinking, and collagen membrane (control) were implanted subcutaneously in the backs of 30 rats. The membranes were analyzed histologically at 2, 4, 8, 12, 16, and 20 weeks. Sections were viewed and graded by a blinded pathologist using a 4‐point scoring system (0 = absent, 1 = mild, 2 = moderate, and 3 = severe) to determine the tissue reaction to the membranes and to observe membrane degradation. There was no statistically significant difference in histological scores among chitosan and collagen membranes at different time points. Absence or minimal inflammation was observed in 57–74% of the membranes across all groups. Most chitosan membranes persisted for 16–20 weeks, whereas most collagen membranes disappeared by resorption at 12–16 weeks. The general tissue response to chitosan nanofiber membranes with and without genipin crosslinking, was similar to that of control commercial collagen membrane. However, the chitosan membranes exhibited slower degradation rates than collagen membranes. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1084–1092, 2014.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Ankit J. Bavariya</name><affiliations><json:string>Department of Periodontology, University of Tennessee Health Science Center, College of Dentistry, Tennessee, Memphis</json:string></affiliations></json:item><json:item><name>P. 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Stein</name><affiliations><json:string>Department of Periodontology, University of Tennessee Health Science Center, College of Dentistry, Tennessee, Memphis</json:string></affiliations></json:item><json:item><name>Joel D. Bumgardner</name><affiliations><json:string>Department of Biomedical Engineering, University of Memphis, Herff College of Engineering, Tennessee, Memphis</json:string><json:string>E-mail: jbmgrdnr@memphis.edu</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>chitosan membrane</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>nanofiber</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>genipin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>biocompatibility</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>degradation</value></json:item></subject><articleId><json:string>JBMB33090</json:string></articleId><arkIstex>ark:/67375/WNG-ZLC3T72N-7</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Chitosan, a natural polysaccharide, has demonstrated potential as a degradable biocompatible guided bone regeneration membrane. This study aimed to evaluate the in vivo biocompatibility and degradation of chitosan nanofiber membranes, with and without genipin crosslinking as compared with a commercial collagen membrane in rat model. Chitosan nanofiber membranes, with and without genipin crosslinking, and collagen membrane (control) were implanted subcutaneously in the backs of 30 rats. The membranes were analyzed histologically at 2, 4, 8, 12, 16, and 20 weeks. Sections were viewed and graded by a blinded pathologist using a 4‐point scoring system (0 = absent, 1 = mild, 2 = moderate, and 3 = severe) to determine the tissue reaction to the membranes and to observe membrane degradation. There was no statistically significant difference in histological scores among chitosan and collagen membranes at different time points. Absence or minimal inflammation was observed in 57–74% of the membranes across all groups. Most chitosan membranes persisted for 16–20 weeks, whereas most collagen membranes disappeared by resorption at 12–16 weeks. The general tissue response to chitosan nanofiber membranes with and without genipin crosslinking, was similar to that of control commercial collagen membrane. However, the chitosan membranes exhibited slower degradation rates than collagen membranes. © 2013 Wiley Periodicals, Inc. 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J.</forename><surname>Bavariya</surname></persName><affiliation><orgName>Department of Periodontology</orgName><orgName>University of Tennessee Health Science Center, College of Dentistry</orgName><address><settlement type="city">Memphis</settlement><region>Tennessee</region><country key="US"></country></address></affiliation></author><author xml:id="author-0001"><persName><genName>Jr.</genName><forename type="first">P.</forename><surname>Andrew Norowski</surname></persName><affiliation><orgName>Department of Biomedical Engineering</orgName><orgName>University of Memphis, Herff College of Engineering</orgName><address><settlement type="city">Memphis</settlement><region>Tennessee</region><country key="US"></country></address></affiliation></author><author xml:id="author-0002"><persName><forename type="first">K.</forename><surname>Mark Anderson</surname></persName><affiliation><orgName>Department of Diagnostic Sciences and Oral Medicine</orgName><orgName>University of Tennessee Health Science Center, College of Dentistry</orgName><address><settlement type="city">Memphis</settlement><region>Tennessee</region><country key="US"></country></address></affiliation></author><author xml:id="author-0003"><persName><forename type="first">Pradeep C.</forename><surname>Adatrow</surname></persName><affiliation><orgName>Department of Prosthodontics</orgName><orgName>University of Tennessee Health Science Center, College of Dentistry</orgName><address><settlement type="city">Memphis</settlement><region>Tennessee</region><country key="US"></country></address></affiliation></author><author xml:id="author-0004"><persName><forename type="first">Franklin</forename><surname>Garcia‐Godoy</surname></persName><affiliation><orgName>Department of Bioscience Research</orgName><orgName>University of Tennessee Health Science Center, College of Dentistry</orgName><address><settlement type="city">Memphis</settlement><region>Tennessee</region><country key="US"></country></address></affiliation></author><author xml:id="author-0005"><persName><forename type="first">Sidney H.</forename><surname>Stein</surname></persName><affiliation><orgName>Department of Periodontology</orgName><orgName>University of Tennessee Health Science Center, College of Dentistry</orgName><address><settlement type="city">Memphis</settlement><region>Tennessee</region><country key="US"></country></address></affiliation></author><author xml:id="author-0006" role="corresp"><persName><forename type="first">Joel D.</forename><surname>Bumgardner</surname></persName><affiliation><orgName>Department of Biomedical Engineering</orgName><orgName>University of Memphis, Herff College of Engineering</orgName><address><settlement type="city">Memphis</settlement><region>Tennessee</region><country key="US"></country></address></affiliation><affiliation>Correspondence to: J.D. Bumgardner (e‐mail: jbmgrdnr@memphis.edu)</affiliation></author><idno type="istex">0B1988332D34DAFFFA1677E9A8C53C92A79C4ABE</idno><idno type="ark">ark:/67375/WNG-ZLC3T72N-7</idno><idno type="DOI">10.1002/jbm.b.33090</idno><idno type="unit">JBMB33090</idno><idno type="toTypesetVersion">file:JBM.JBMB33090.pdf</idno></analytic><monogr><title level="j" type="main">Journal of Biomedical Materials Research Part B: Applied Biomaterials</title><title level="j" type="alt">JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B: APPLIED BIOMATERIALS</title><idno type="pISSN">1552-4973</idno><idno type="eISSN">1552-4981</idno><idno type="book-DOI">10.1002/(ISSN)1552-4981</idno><idno type="book-part-DOI">10.1002/jbm.b.v102.5</idno><idno type="product">JBM</idno><imprint><biblScope unit="vol">102</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="1084">1084</biblScope><biblScope unit="page" to="1092">1092</biblScope><biblScope unit="page-count">9</biblScope><date type="published" when="2014-07"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><abstract style="main"><head>Abstract</head><p>Chitosan, a natural polysaccharide, has demonstrated potential as a degradable biocompatible guided bone regeneration membrane. This study aimed to evaluate the <hi rend="italic">in vivo</hi> biocompatibility and degradation of chitosan nanofiber membranes, with and without genipin crosslinking as compared with a commercial collagen membrane in rat model. Chitosan nanofiber membranes, with and without genipin crosslinking, and collagen membrane (control) were implanted subcutaneously in the backs of 30 rats. The membranes were analyzed histologically at 2, 4, 8, 12, 16, and 20 weeks. Sections were viewed and graded by a blinded pathologist using a 4‐point scoring system (0 = absent, 1 = mild, 2 = moderate, and 3 = severe) to determine the tissue reaction to the membranes and to observe membrane degradation. There was no statistically significant difference in histological scores among chitosan and collagen membranes at different time points. Absence or minimal inflammation was observed in 57–74% of the membranes across all groups. Most chitosan membranes persisted for 16–20 weeks, whereas most collagen membranes disappeared by resorption at 12–16 weeks. The general tissue response to chitosan nanofiber membranes with and without genipin crosslinking, was similar to that of control commercial collagen membrane. However, the chitosan membranes exhibited slower degradation rates than collagen membranes. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1084–1092, 2014.</p></abstract><textClass><keywords><term xml:id="jbmb33090-kwd-0001">chitosan membrane</term><term xml:id="jbmb33090-kwd-0002">nanofiber</term><term xml:id="jbmb33090-kwd-0003">genipin</term><term xml:id="jbmb33090-kwd-0004">biocompatibility</term><term xml:id="jbmb33090-kwd-0005">degradation</term></keywords><keywords rend="articleCategory"><term>Original Report</term></keywords><keywords rend="tocHeading1"><term>Original Research Reports</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en" xml:id="jbmb33090"><header><publicationMeta level="product"><doi origin="wiley" registered="yes">10.1002/(ISSN)1552-4981</doi><issn type="print">1552-4973</issn><issn type="electronic">1552-4981</issn><idGroup><id type="product" value="JBM"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B: APPLIED BIOMATERIALS">Journal of Biomedical Materials Research Part B: Applied Biomaterials</title><title type="short">J. 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Bumgardner (e‐mail: <email>jbmgrdnr@memphis.edu</email>)</correspondenceTo><selfCitationGroup><citation xml:id="jbmb33090-cit-0048" type="self"><b>How to cite this article:</b>
<author><familyName>Bavariya</familyName> <givenNames>AJ</givenNames></author>,
<author><familyName>Andrew Norowski</familyName> <givenNames>P</givenNames></author>,
<author><familyName>Mark Anderson</familyName> <givenNames>K</givenNames></author>,
<author><familyName>Adatrow</familyName> <givenNames>PC</givenNames></author>,
<author><familyName>Garcia‐Godoy</familyName> <givenNames>F</givenNames></author>,
<author><familyName>Stein</familyName> <givenNames>SH</givenNames></author>,
<author><familyName>Bumgardner</familyName> <givenNames>JD</givenNames></author>. <pubYear year="2014">2014</pubYear>. <articleTitle>Evaluation of biocompatibility and degradation of chitosan nanofiber membrane crosslinked with genipin</articleTitle>. <journalTitle>J Biomed Mater Res Part B</journalTitle> 2014:<vol>102B</vol>:<pageFirst>1084</pageFirst>–<pageLast>1092</pageLast>.
</citation></selfCitationGroup><linkGroup><link type="toTypesetVersion" href="file:JBM.JBMB33090.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Evaluation of biocompatibility and degradation of chitosan nanofiber membrane crosslinked with genipin</title><title type="short">Biocompatibility and Degradation of Nanofiber Chitosan Membranes</title><title type="shortAuthors">Ankit et al.</title></titleGroup><creators><creator affiliationRef="#jbmb33090-aff-0001" creatorRole="author" xml:id="jbmb33090-cr-0001"><personName><givenNames>Ankit J.</givenNames><familyName>Bavariya</familyName></personName></creator><creator affiliationRef="#jbmb33090-aff-0002" creatorRole="author" xml:id="jbmb33090-cr-0002"><personName><givenNames>P.</givenNames><familyName>Andrew Norowski</familyName><nameSuffix>Jr.</nameSuffix></personName></creator><creator affiliationRef="#jbmb33090-aff-0003" creatorRole="author" xml:id="jbmb33090-cr-0003"><personName><givenNames>K.</givenNames><familyName>Mark Anderson</familyName></personName></creator><creator affiliationRef="#jbmb33090-aff-0004" creatorRole="author" xml:id="jbmb33090-cr-0004"><personName><givenNames>Pradeep C.</givenNames><familyName>Adatrow</familyName></personName></creator><creator affiliationRef="#jbmb33090-aff-0005" creatorRole="author" xml:id="jbmb33090-cr-0005"><personName><givenNames>Franklin</givenNames><familyName>Garcia‐Godoy</familyName></personName></creator><creator affiliationRef="#jbmb33090-aff-0001" creatorRole="author" xml:id="jbmb33090-cr-0006"><personName><givenNames>Sidney H.</givenNames><familyName>Stein</familyName></personName></creator><creator affiliationRef="#jbmb33090-aff-0002" corresponding="yes" creatorRole="author" xml:id="jbmb33090-cr-0007"><personName><givenNames>Joel D.</givenNames><familyName>Bumgardner</familyName></personName></creator></creators><affiliationGroup><affiliation countryCode="US" type="organization" xml:id="jbmb33090-aff-0001"><orgDiv>Department of Periodontology</orgDiv><orgName>University of Tennessee Health Science Center, College of Dentistry</orgName><address><city>Memphis</city><countryPart>Tennessee</countryPart></address></affiliation><affiliation countryCode="US" type="organization" xml:id="jbmb33090-aff-0002"><orgDiv>Department of Biomedical Engineering</orgDiv><orgName>University of Memphis, Herff College of Engineering</orgName><address><city>Memphis</city><countryPart>Tennessee</countryPart></address></affiliation><affiliation countryCode="US" type="organization" xml:id="jbmb33090-aff-0003"><orgDiv>Department of Diagnostic Sciences and Oral Medicine</orgDiv><orgName>University of Tennessee Health Science Center, College of Dentistry</orgName><address><city>Memphis</city><countryPart>Tennessee</countryPart></address></affiliation><affiliation countryCode="US" type="organization" xml:id="jbmb33090-aff-0004"><orgDiv>Department of Prosthodontics</orgDiv><orgName>University of Tennessee Health Science Center, College of Dentistry</orgName><address><city>Memphis</city><countryPart>Tennessee</countryPart></address></affiliation><affiliation countryCode="US" type="organization" xml:id="jbmb33090-aff-0005"><orgDiv>Department of Bioscience Research</orgDiv><orgName>University of Tennessee Health Science Center, College of Dentistry</orgName><address><city>Memphis</city><countryPart>Tennessee</countryPart></address></affiliation></affiliationGroup><keywordGroup type="author"><keyword xml:id="jbmb33090-kwd-0001">chitosan membrane</keyword><keyword xml:id="jbmb33090-kwd-0002">nanofiber</keyword><keyword xml:id="jbmb33090-kwd-0003">genipin</keyword><keyword xml:id="jbmb33090-kwd-0004">biocompatibility</keyword><keyword xml:id="jbmb33090-kwd-0005">degradation</keyword></keywordGroup><fundingInfo><fundingAgency>Alumni Endowment Fund of the University of Tennessee, College of Dentistry
</fundingAgency></fundingInfo><abstractGroup><abstract type="main"><title type="main">Abstract</title><p>Chitosan, a natural polysaccharide, has demonstrated potential as a degradable biocompatible guided bone regeneration membrane. This study aimed to evaluate the <i>in vivo</i> biocompatibility and degradation of chitosan nanofiber membranes, with and without genipin crosslinking as compared with a commercial collagen membrane in rat model. Chitosan nanofiber membranes, with and without genipin crosslinking, and collagen membrane (control) were implanted subcutaneously in the backs of 30 rats. The membranes were analyzed histologically at 2, 4, 8, 12, 16, and 20 weeks. Sections were viewed and graded by a blinded pathologist using a 4‐point scoring system (0 = absent, 1 = mild, 2 = moderate, and 3 = severe) to determine the tissue reaction to the membranes and to observe membrane degradation. There was no statistically significant difference in histological scores among chitosan and collagen membranes at different time points. Absence or minimal inflammation was observed in 57–74% of the membranes across all groups. Most chitosan membranes persisted for 16–20 weeks, whereas most collagen membranes disappeared by resorption at 12–16 weeks. The general tissue response to chitosan nanofiber membranes with and without genipin crosslinking, was similar to that of control commercial collagen membrane. However, the chitosan membranes exhibited slower degradation rates than collagen membranes. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1084–1092, 2014.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Evaluation of biocompatibility and degradation of chitosan nanofiber membrane crosslinked with genipin</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Biocompatibility and Degradation of Nanofiber Chitosan Membranes</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Evaluation of biocompatibility and degradation of chitosan nanofiber membrane crosslinked with genipin</title></titleInfo><name type="personal"><namePart type="given">Ankit J.</namePart><namePart type="family">Bavariya</namePart><affiliation>Department of Periodontology, University of Tennessee Health Science Center, College of Dentistry, Tennessee, Memphis</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Andrew Norowski Jr.</namePart><affiliation>Department of Biomedical Engineering, University of Memphis, Herff College of Engineering, Tennessee, Memphis</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Mark Anderson</namePart><affiliation>Department of Diagnostic Sciences and Oral Medicine, University of Tennessee Health Science Center, College of Dentistry, Tennessee, Memphis</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pradeep C.</namePart><namePart type="family">Adatrow</namePart><affiliation>Department of Prosthodontics, University of Tennessee Health Science Center, College of Dentistry, Tennessee, Memphis</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Franklin</namePart><namePart type="family">Garcia‐Godoy</namePart><affiliation>Department of Bioscience Research, University of Tennessee Health Science Center, College of Dentistry, Tennessee, Memphis</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sidney H.</namePart><namePart type="family">Stein</namePart><affiliation>Department of Periodontology, University of Tennessee Health Science Center, College of Dentistry, Tennessee, Memphis</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Joel D.</namePart><namePart type="family">Bumgardner</namePart><affiliation>Department of Biomedical Engineering, University of Memphis, Herff College of Engineering, Tennessee, Memphis</affiliation><affiliation>E-mail: jbmgrdnr@memphis.edu</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><dateIssued encoding="w3cdtf">2014-07</dateIssued><dateCreated encoding="w3cdtf">2013-11-29</dateCreated><dateCaptured encoding="w3cdtf">2013-05-20</dateCaptured><dateValid encoding="w3cdtf">2013-11-16</dateValid><edition>How to cite this article: Bavariya AJ, Andrew Norowski P, Mark Anderson K, Adatrow PC, Garcia‐Godoy F, Stein SH, Bumgardner JD. 2014. Evaluation of biocompatibility and degradation of chitosan nanofiber membrane crosslinked with genipin. J Biomed Mater Res Part B 2014:102B:1084–1092.</edition><copyrightDate encoding="w3cdtf">2014</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract>Chitosan, a natural polysaccharide, has demonstrated potential as a degradable biocompatible guided bone regeneration membrane. This study aimed to evaluate the in vivo biocompatibility and degradation of chitosan nanofiber membranes, with and without genipin crosslinking as compared with a commercial collagen membrane in rat model. Chitosan nanofiber membranes, with and without genipin crosslinking, and collagen membrane (control) were implanted subcutaneously in the backs of 30 rats. The membranes were analyzed histologically at 2, 4, 8, 12, 16, and 20 weeks. Sections were viewed and graded by a blinded pathologist using a 4‐point scoring system (0 = absent, 1 = mild, 2 = moderate, and 3 = severe) to determine the tissue reaction to the membranes and to observe membrane degradation. There was no statistically significant difference in histological scores among chitosan and collagen membranes at different time points. Absence or minimal inflammation was observed in 57–74% of the membranes across all groups. Most chitosan membranes persisted for 16–20 weeks, whereas most collagen membranes disappeared by resorption at 12–16 weeks. The general tissue response to chitosan nanofiber membranes with and without genipin crosslinking, was similar to that of control commercial collagen membrane. However, the chitosan membranes exhibited slower degradation rates than collagen membranes. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1084–1092, 2014.</abstract><note type="funding">Alumni Endowment Fund of the University of Tennessee, College of Dentistry</note><subject><genre>keywords</genre><topic>chitosan membrane</topic><topic>nanofiber</topic><topic>genipin</topic><topic>biocompatibility</topic><topic>degradation</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Biomedical Materials Research Part B: Applied Biomaterials</title></titleInfo><titleInfo type="abbreviated"><title>J. Biomed. Mater. Res.</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><subject><genre>article-category</genre><topic>Original Report</topic></subject><identifier type="ISSN">1552-4973</identifier><identifier type="eISSN">1552-4981</identifier><identifier type="DOI">10.1002/(ISSN)1552-4981</identifier><identifier type="PublisherID">JBM</identifier><part><date>2014</date><detail type="volume"><caption>vol.</caption><number>102</number></detail><detail type="issue"><caption>no.</caption><number>5</number></detail><extent unit="pages"><start>1084</start><end>1092</end><total>9</total></extent></part></relatedItem><identifier type="istex">0B1988332D34DAFFFA1677E9A8C53C92A79C4ABE</identifier><identifier type="ark">ark:/67375/WNG-ZLC3T72N-7</identifier><identifier type="DOI">10.1002/jbm.b.33090</identifier><identifier type="ArticleID">JBMB33090</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2013 Wiley Periodicals, Inc.© 2013 Wiley Periodicals, Inc.</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/0B1988332D34DAFFFA1677E9A8C53C92A79C4ABE/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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