RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak.
Identifieur interne : 000B98 ( PubMed/Corpus ); précédent : 000B97; suivant : 000B99RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak.
Auteurs : Liangjun Chen ; Weiyong Liu ; Qi Zhang ; Ke Xu ; Guangming Ye ; Weichen Wu ; Ziyong Sun ; Fang Liu ; Kailang Wu ; Bo Zhong ; Yi Mei ; Wenxia Zhang ; Yu Chen ; Yirong Li ; Mang Shi ; Ke Lan ; Yingle LiuSource :
- Emerging microbes & infections [ 2222-1751 ] ; 2020.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : RNA, Viral.
- geographic : China.
- epidemiology : Coronavirus Infections, Pneumonia, Viral.
- genetics : Betacoronavirus.
- Adult, Disease Outbreaks, Female, Genome, Viral, Humans, Male, Phylogeny, Young Adult.
Abstract
From December 2019, an outbreak of unusual pneumonia was reported in Wuhan with many cases linked to Huanan Seafood Market that sells seafood as well as live exotic animals. We investigated two patients who developed acute respiratory syndromes after independent contact history with this market. The two patients shared common clinical features including fever, cough, and multiple ground-glass opacities in the bilateral lung field with patchy infiltration. Here, we highlight the use of a low-input metagenomic next-generation sequencing (mNGS) approach on RNA extracted from bronchoalveolar lavage fluid (BALF). It rapidly identified a novel coronavirus (named 2019-nCoV according to World Health Organization announcement) which was the sole pathogens in the sample with very high abundance level (1.5% and 0.62% of total RNA sequenced). The entire viral genome is 29,881 nt in length (GenBank MN988668 and MN988669, Sequence Read Archive database Bioproject accession PRJNA601736) and is classified into β-coronavirus genus. Phylogenetic analysis indicates that 2019-nCoV is close to coronaviruses (CoVs) circulating in Rhinolophus (Horseshoe bats), such as 98.7% nucleotide identity to partial RdRp gene of bat coronavirus strain BtCoV/4991 (GenBank KP876546, 370 nt sequence of RdRp and lack of other genome sequence) and 87.9% nucleotide identity to bat coronavirus strain bat-SL-CoVZC45 and bat-SL-CoVZXC21. Evolutionary analysis based on ORF1a/1b, S, and N genes also suggests 2019-nCoV is more likely a novel CoV independently introduced from animals to humans.
DOI: 10.1080/22221751.2020.1725399
PubMed: 32020836
Links to Exploration step
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Zhang</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Ye, Guangming" sort="Ye, Guangming" uniqKey="Ye G" first="Guangming" last="Ye">Guangming Ye</name><affiliation><nlm:affiliation>Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Weichen" sort="Wu, Weichen" uniqKey="Wu W" first="Weichen" last="Wu">Weichen Wu</name><affiliation><nlm:affiliation>School of Medicine, Sun yat-Sen University, Guangzhou, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Sun, Ziyong" sort="Sun, Ziyong" uniqKey="Sun Z" first="Ziyong" last="Sun">Ziyong Sun</name><affiliation><nlm:affiliation>Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Fang" sort="Liu, Fang" uniqKey="Liu F" first="Fang" last="Liu">Fang Liu</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Kailang" sort="Wu, Kailang" uniqKey="Wu K" first="Kailang" last="Wu">Kailang Wu</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhong, Bo" sort="Zhong, Bo" uniqKey="Zhong B" first="Bo" last="Zhong">Bo Zhong</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Mei, Yi" sort="Mei, Yi" uniqKey="Mei Y" first="Yi" last="Mei">Yi Mei</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Wenxia" sort="Zhang, Wenxia" uniqKey="Zhang W" first="Wenxia" last="Zhang">Wenxia Zhang</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Yu" sort="Chen, Yu" uniqKey="Chen Y" first="Yu" last="Chen">Yu Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yirong" sort="Li, Yirong" uniqKey="Li Y" first="Yirong" last="Li">Yirong Li</name><affiliation><nlm:affiliation>Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Shi, Mang" sort="Shi, Mang" uniqKey="Shi M" first="Mang" last="Shi">Mang Shi</name><affiliation><nlm:affiliation>School of Medicine, Sun yat-Sen University, Guangzhou, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Lan, Ke" sort="Lan, Ke" uniqKey="Lan K" first="Ke" last="Lan">Ke Lan</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Yingle" sort="Liu, Yingle" uniqKey="Liu Y" first="Yingle" last="Liu">Yingle Liu</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32020836</idno><idno type="pmid">32020836</idno><idno type="doi">10.1080/22221751.2020.1725399</idno><idno type="wicri:Area/PubMed/Corpus">000B98</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000B98</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak.</title><author><name sortKey="Chen, Liangjun" sort="Chen, Liangjun" uniqKey="Chen L" first="Liangjun" last="Chen">Liangjun Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Weiyong" sort="Liu, Weiyong" uniqKey="Liu W" first="Weiyong" last="Liu">Weiyong Liu</name><affiliation><nlm:affiliation>Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Qi" sort="Zhang, Qi" uniqKey="Zhang Q" first="Qi" last="Zhang">Qi Zhang</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Ye, Guangming" sort="Ye, Guangming" uniqKey="Ye G" first="Guangming" last="Ye">Guangming Ye</name><affiliation><nlm:affiliation>Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Weichen" sort="Wu, Weichen" uniqKey="Wu W" first="Weichen" last="Wu">Weichen Wu</name><affiliation><nlm:affiliation>School of Medicine, Sun yat-Sen University, Guangzhou, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Sun, Ziyong" sort="Sun, Ziyong" uniqKey="Sun Z" first="Ziyong" last="Sun">Ziyong Sun</name><affiliation><nlm:affiliation>Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Fang" sort="Liu, Fang" uniqKey="Liu F" first="Fang" last="Liu">Fang Liu</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Kailang" sort="Wu, Kailang" uniqKey="Wu K" first="Kailang" last="Wu">Kailang Wu</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhong, Bo" sort="Zhong, Bo" uniqKey="Zhong B" first="Bo" last="Zhong">Bo Zhong</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Mei, Yi" sort="Mei, Yi" uniqKey="Mei Y" first="Yi" last="Mei">Yi Mei</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Wenxia" sort="Zhang, Wenxia" uniqKey="Zhang W" first="Wenxia" last="Zhang">Wenxia Zhang</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Yu" sort="Chen, Yu" uniqKey="Chen Y" first="Yu" last="Chen">Yu Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Yirong" sort="Li, Yirong" uniqKey="Li Y" first="Yirong" last="Li">Yirong Li</name><affiliation><nlm:affiliation>Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Shi, Mang" sort="Shi, Mang" uniqKey="Shi M" first="Mang" last="Shi">Mang Shi</name><affiliation><nlm:affiliation>School of Medicine, Sun yat-Sen University, Guangzhou, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Lan, Ke" sort="Lan, Ke" uniqKey="Lan K" first="Ke" last="Lan">Ke Lan</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Yingle" sort="Liu, Yingle" uniqKey="Liu Y" first="Yingle" last="Liu">Yingle Liu</name><affiliation><nlm:affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Emerging microbes & infections</title><idno type="eISSN">2222-1751</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Betacoronavirus (genetics)</term><term>China</term><term>Coronavirus Infections (epidemiology)</term><term>Disease Outbreaks</term><term>Female</term><term>Genome, Viral</term><term>Humans</term><term>Male</term><term>Phylogeny</term><term>Pneumonia, Viral (epidemiology)</term><term>RNA, Viral (genetics)</term><term>Young Adult</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Betacoronavirus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Disease Outbreaks</term><term>Female</term><term>Genome, Viral</term><term>Humans</term><term>Male</term><term>Phylogeny</term><term>Young Adult</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">From December 2019, an outbreak of unusual pneumonia was reported in Wuhan with many cases linked to Huanan Seafood Market that sells seafood as well as live exotic animals. We investigated two patients who developed acute respiratory syndromes after independent contact history with this market. The two patients shared common clinical features including fever, cough, and multiple ground-glass opacities in the bilateral lung field with patchy infiltration. Here, we highlight the use of a low-input metagenomic next-generation sequencing (mNGS) approach on RNA extracted from bronchoalveolar lavage fluid (BALF). It rapidly identified a novel coronavirus (named 2019-nCoV according to World Health Organization announcement) which was the sole pathogens in the sample with very high abundance level (1.5% and 0.62% of total RNA sequenced). The entire viral genome is 29,881 nt in length (GenBank MN988668 and MN988669, Sequence Read Archive database Bioproject accession PRJNA601736) and is classified into β-coronavirus genus. Phylogenetic analysis indicates that 2019-nCoV is close to coronaviruses (CoVs) circulating in Rhinolophus (Horseshoe bats), such as 98.7% nucleotide identity to partial RdRp gene of bat coronavirus strain BtCoV/4991 (GenBank KP876546, 370 nt sequence of RdRp and lack of other genome sequence) and 87.9% nucleotide identity to bat coronavirus strain bat-SL-CoVZC45 and bat-SL-CoVZXC21. Evolutionary analysis based on ORF1a/1b, S, and N genes also suggests 2019-nCoV is more likely a novel CoV independently introduced from animals to humans.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32020836</PMID><DateCompleted><Year>2020</Year><Month>03</Month><Day>16</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>25</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">2222-1751</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>1</Issue><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Emerging microbes & infections</Title><ISOAbbreviation>Emerg Microbes Infect</ISOAbbreviation></Journal><ArticleTitle>RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak.</ArticleTitle><Pagination><MedlinePgn>313-319</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/22221751.2020.1725399</ELocationID><Abstract><AbstractText>From December 2019, an outbreak of unusual pneumonia was reported in Wuhan with many cases linked to Huanan Seafood Market that sells seafood as well as live exotic animals. We investigated two patients who developed acute respiratory syndromes after independent contact history with this market. The two patients shared common clinical features including fever, cough, and multiple ground-glass opacities in the bilateral lung field with patchy infiltration. Here, we highlight the use of a low-input metagenomic next-generation sequencing (mNGS) approach on RNA extracted from bronchoalveolar lavage fluid (BALF). It rapidly identified a novel coronavirus (named 2019-nCoV according to World Health Organization announcement) which was the sole pathogens in the sample with very high abundance level (1.5% and 0.62% of total RNA sequenced). The entire viral genome is 29,881 nt in length (GenBank MN988668 and MN988669, Sequence Read Archive database Bioproject accession PRJNA601736) and is classified into β-coronavirus genus. Phylogenetic analysis indicates that 2019-nCoV is close to coronaviruses (CoVs) circulating in Rhinolophus (Horseshoe bats), such as 98.7% nucleotide identity to partial RdRp gene of bat coronavirus strain BtCoV/4991 (GenBank KP876546, 370 nt sequence of RdRp and lack of other genome sequence) and 87.9% nucleotide identity to bat coronavirus strain bat-SL-CoVZC45 and bat-SL-CoVZXC21. Evolutionary analysis based on ORF1a/1b, S, and N genes also suggests 2019-nCoV is more likely a novel CoV independently introduced from animals to humans.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Liangjun</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Weiyong</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Qi</ForeName><Initials>Q</Initials><Identifier Source="ORCID">0000-0003-2868-1816</Identifier><AffiliationInfo><Affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Ke</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Guangming</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Weichen</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>School of Medicine, Sun yat-Sen University, Guangzhou, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Ziyong</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Fang</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Kailang</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhong</LastName><ForeName>Bo</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mei</LastName><ForeName>Yi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Wenxia</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yu</ForeName><Initials>Y</Initials><Identifier Source="ORCID">0000-0003-1300-4652</Identifier><AffiliationInfo><Affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yirong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Mang</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>School of Medicine, Sun yat-Sen University, Guangzhou, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lan</LastName><ForeName>Ke</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yingle</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D002363">Case Reports</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>02</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Emerg Microbes 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