Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.
Identifieur interne : 000387 ( PubMed/Checkpoint ); précédent : 000386; suivant : 000388Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.
Auteurs : Regina Konrad ; Ute Eberle [Allemagne] ; Alexandra Dangel ; Bianca Treis [Allemagne] ; Anja Berger [Allemagne] ; Katja Bengs [Allemagne] ; Volker Fingerle [Allemagne] ; Bernhard Liebl [Allemagne] ; Nikolaus Ackermann [Allemagne] ; Andreas Sing [Allemagne]Source :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [ 1560-7917 ] ; 2020.
Descripteurs français
- KwdFr :
- Allemagne, Facteurs temps, Flux de travaux, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (génétique), Pneumopathie virale (diagnostic), Pneumopathie virale (génétique), Protéines de l'enveloppe virale (analyse), Protéines de l'enveloppe virale (génétique), Réaction de polymérisation en chaine en temps réel (), Sensibilité et spécificité, Séquençage par oligonucléotides en batterie.
- MESH :
- analyse : Protéines de l'enveloppe virale.
- diagnostic : Infections à coronavirus, Pneumopathie virale.
- génétique : Infections à coronavirus, Pneumopathie virale, Protéines de l'enveloppe virale.
- Allemagne, Facteurs temps, Flux de travaux, Humains, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Séquençage par oligonucléotides en batterie.
- Wicri :
- geographic : Allemagne.
English descriptors
- KwdEn :
- Betacoronavirus (genetics), Betacoronavirus (isolation & purification), Coronavirus Infections (diagnosis), Coronavirus Infections (genetics), Germany, Humans, Oligonucleotide Array Sequence Analysis, Pneumonia, Viral (diagnosis), Pneumonia, Viral (genetics), Real-Time Polymerase Chain Reaction (methods), Sensitivity and Specificity, Time Factors, Viral Envelope Proteins (analysis), Viral Envelope Proteins (genetics), Workflow.
- MESH :
- chemical , analysis : Viral Envelope Proteins.
- chemical , genetics : Viral Envelope Proteins.
- geographic : Germany.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- genetics : Betacoronavirus, Coronavirus Infections, Pneumonia, Viral.
- isolation & purification : Betacoronavirus.
- methods : Real-Time Polymerase Chain Reaction.
- Humans, Oligonucleotide Array Sequence Analysis, Sensitivity and Specificity, Time Factors, Workflow.
Abstract
The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.
DOI: 10.2807/1560-7917.ES.2020.25.9.2000173
PubMed: 32156330
Affiliations:
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pubmed:32156330Le document en format XML
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sortKey="Fingerle, Volker" sort="Fingerle, Volker" uniqKey="Fingerle V" first="Volker" last="Fingerle">Volker Fingerle</name><affiliation wicri:level="1"><nlm:affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion></affiliation></author><author><name sortKey="Liebl, Bernhard" sort="Liebl, Bernhard" uniqKey="Liebl B" first="Bernhard" last="Liebl">Bernhard Liebl</name><affiliation wicri:level="3"><nlm:affiliation>Ludwig Maximilians-Universität, Munich, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ludwig Maximilians-Universität, Munich</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region><settlement type="city">Munich</settlement></placeName></affiliation></author><author><name sortKey="Ackermann, Nikolaus" sort="Ackermann, Nikolaus" uniqKey="Ackermann N" first="Nikolaus" last="Ackermann">Nikolaus Ackermann</name><affiliation wicri:level="1"><nlm:affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion></affiliation></author><author><name sortKey="Sing, Andreas" sort="Sing, Andreas" uniqKey="Sing A" first="Andreas" last="Sing">Andreas Sing</name><affiliation wicri:level="3"><nlm:affiliation>Ludwig Maximilians-Universität, Munich, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ludwig Maximilians-Universität, Munich</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region><settlement type="city">Munich</settlement></placeName></affiliation></author></analytic><series><title level="j">Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</title><idno type="eISSN">1560-7917</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Betacoronavirus (genetics)</term><term>Betacoronavirus (isolation & purification)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (genetics)</term><term>Germany</term><term>Humans</term><term>Oligonucleotide Array Sequence Analysis</term><term>Pneumonia, Viral (diagnosis)</term><term>Pneumonia, Viral (genetics)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term><term>Time Factors</term><term>Viral Envelope Proteins (analysis)</term><term>Viral Envelope Proteins (genetics)</term><term>Workflow</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Allemagne</term><term>Facteurs temps</term><term>Flux de travaux</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (génétique)</term><term>Pneumopathie virale (diagnostic)</term><term>Pneumopathie virale (génétique)</term><term>Protéines de l'enveloppe virale (analyse)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Sensibilité et spécificité</term><term>Séquençage par oligonucléotides en batterie</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Germany</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term><term>Pneumopathie virale</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Betacoronavirus</term><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Infections à coronavirus</term><term>Pneumopathie virale</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Betacoronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Oligonucleotide Array Sequence Analysis</term><term>Sensitivity and Specificity</term><term>Time Factors</term><term>Workflow</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Allemagne</term><term>Facteurs temps</term><term>Flux de travaux</term><term>Humains</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Séquençage par oligonucléotides en batterie</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Allemagne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32156330</PMID><DateCompleted><Year>2020</Year><Month>03</Month><Day>18</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1560-7917</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>9</Issue><PubDate><Year>2020</Year><Month>03</Month></PubDate></JournalIssue><Title>Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin</Title><ISOAbbreviation>Euro Surveill.</ISOAbbreviation></Journal><ArticleTitle>Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.2807/1560-7917.ES.2020.25.9.2000173</ELocationID><Abstract><AbstractText>The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Konrad</LastName><ForeName>Regina</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>These authors contributed equally to this work.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eberle</LastName><ForeName>Ute</ForeName><Initials>U</Initials><AffiliationInfo><Affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>These authors contributed equally to this work.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dangel</LastName><ForeName>Alexandra</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>These authors contributed equally to this work.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Treis</LastName><ForeName>Bianca</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Berger</LastName><ForeName>Anja</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bengs</LastName><ForeName>Katja</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fingerle</LastName><ForeName>Volker</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liebl</LastName><ForeName>Bernhard</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Ludwig Maximilians-Universität, Munich, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>State Institute of Health, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ackermann</LastName><ForeName>Nikolaus</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>These authors contributed equally to this work.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sing</LastName><ForeName>Andreas</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Ludwig Maximilians-Universität, Munich, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>These authors contributed equally to this work.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Sweden</Country><MedlineTA>Euro Surveill</MedlineTA><NlmUniqueID>100887452</NlmUniqueID><ISSNLinking>1025-496X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical></ChemicalList><SupplMeshList><SupplMeshName Type="Disease" UI="C000657245">COVID-19</SupplMeshName><SupplMeshName Type="Organism" UI="C000656484">severe acute respiratory syndrome coronavirus 2</SupplMeshName></SupplMeshList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000073640" MajorTopicYN="Y">Betacoronavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="Y">Coronavirus Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005858" MajorTopicYN="N" Type="Geographic">Germany</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020411" MajorTopicYN="N">Oligonucleotide Array Sequence Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011024" MajorTopicYN="Y">Pneumonia, Viral</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057188" MajorTopicYN="N">Workflow</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">COVID-19</Keyword><Keyword MajorTopicYN="Y">Corona Virus</Keyword><Keyword MajorTopicYN="Y">Infectious disease</Keyword><Keyword MajorTopicYN="Y">Laboratory Diagnostics</Keyword><Keyword MajorTopicYN="Y">Molecular Testing</Keyword><Keyword MajorTopicYN="Y">Real-Time PCR</Keyword><Keyword MajorTopicYN="Y">SARS-CoV-2</Keyword><Keyword MajorTopicYN="Y">outbreaks</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>3</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>3</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32156330</ArticleId><ArticleId IdType="doi">10.2807/1560-7917.ES.2020.25.9.2000173</ArticleId><ArticleId IdType="pmc">PMC7068163</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Virol Methods. 2006 Mar;132(1-2):166-73</Citation><ArticleIdList><ArticleId IdType="pubmed">16274752</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Euro Surveill. 2020 Jan;25(3):</Citation><ArticleIdList><ArticleId IdType="pubmed">31992387</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Euro Surveill. 2020 Feb;25(6):</Citation><ArticleIdList><ArticleId IdType="pubmed">32046815</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Chin Med J (Engl). 2020 Feb 11;:</Citation><ArticleIdList><ArticleId IdType="pubmed">32004165</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antiviral Res. 2018 Oct;158:127-134</Citation><ArticleIdList><ArticleId IdType="pubmed">30059721</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>N Engl J Med. 2020 Mar 5;382(10):970-971</Citation><ArticleIdList><ArticleId IdType="pubmed">32003551</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 2020 Mar;579(7798):265-269</Citation><ArticleIdList><ArticleId IdType="pubmed">32015508</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Bavière</li><li>District de Haute-Bavière</li></region><settlement><li>Munich</li></settlement></list><tree><noCountry><name sortKey="Dangel, Alexandra" sort="Dangel, Alexandra" uniqKey="Dangel A" first="Alexandra" last="Dangel">Alexandra Dangel</name><name sortKey="Konrad, Regina" sort="Konrad, Regina" uniqKey="Konrad R" first="Regina" last="Konrad">Regina Konrad</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Eberle, Ute" sort="Eberle, Ute" uniqKey="Eberle U" first="Ute" last="Eberle">Ute Eberle</name></noRegion><name sortKey="Ackermann, Nikolaus" sort="Ackermann, Nikolaus" uniqKey="Ackermann N" first="Nikolaus" last="Ackermann">Nikolaus Ackermann</name><name sortKey="Bengs, Katja" sort="Bengs, Katja" uniqKey="Bengs K" first="Katja" last="Bengs">Katja Bengs</name><name sortKey="Berger, Anja" sort="Berger, Anja" uniqKey="Berger A" first="Anja" last="Berger">Anja Berger</name><name sortKey="Fingerle, Volker" sort="Fingerle, Volker" uniqKey="Fingerle V" first="Volker" last="Fingerle">Volker Fingerle</name><name sortKey="Liebl, Bernhard" sort="Liebl, Bernhard" uniqKey="Liebl B" first="Bernhard" last="Liebl">Bernhard Liebl</name><name sortKey="Sing, Andreas" sort="Sing, Andreas" uniqKey="Sing A" first="Andreas" last="Sing">Andreas Sing</name><name sortKey="Treis, Bianca" sort="Treis, Bianca" uniqKey="Treis B" first="Bianca" last="Treis">Bianca Treis</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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