Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction
Identifieur interne : 000A38 ( Pmc/Curation ); précédent : 000A37; suivant : 000A39Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction
Auteurs : Mohamed S. Al-Ayed ; Ahmed M. Asaad ; Mohamed A. Qureshi ; Mohammed S. AmeenSource :
- Saudi Medical Journal [ 0379-5284 ] ; 2014.
Abstract
To investigate 15 respiratory viruses in children with acute respiratory tract infections (ARTIs) using multiplex reverse-transcriptase polymerase chain reaction (RT-PCR), and to analyze the clinical and epidemiological features of these viruses.
In a cross-sectional study, 135 children, ≤5 years of age who presented with ARTIs in Najran Maternity and Children Hospital, Najran, Saudi Arabia between October 2012 and July 2013 were included. The clinical and sociodemographic data, and the laboratory results were recorded using a standardized questionnaire. Two nasopharyngeal swabs were collected from each child: one for bacteriological examination, and the second for viral detection using multiplex RT-PCR.
A single viral pathogen was detected in 76 patients, viral coinfections in 9, and mixed viral and bacterial pathogens in 15. Respiratory syncytial virus was isolated in 33 patients, human rhinovirus (hRV) in 22, adenovirus (AdV) in 19, human metapneumovirus in 13, influenza virus in 10, parainfluenza virus in 7, human corona virus (hCoV) in 4, and human bocavirus in one.
Respiratory syncytial virus, hRV, and AdV were the most frequent viruses, accounting for more than two-thirds of the cases. Other viruses, such as MPV, hCoV NL63, and hCoV OC43, may play a role in pediatric ARTIs. Of significance is the potential use of multiplex RT-PCR to provide epidemiological and virological data for early detection of the emergence of novel respiratory viruses in the era of the Middle East respiratory syndrome coronavirus.
Url:
PubMed: 25399211
PubMed Central: 4362149
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<author><name sortKey="Al Ayed, Mohamed S" sort="Al Ayed, Mohamed S" uniqKey="Al Ayed M" first="Mohamed S." last="Al-Ayed">Mohamed S. Al-Ayed</name>
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<author><name sortKey="Qureshi, Mohamed A" sort="Qureshi, Mohamed A" uniqKey="Qureshi M" first="Mohamed A." last="Qureshi">Mohamed A. Qureshi</name>
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<author><name sortKey="Ameen, Mohammed S" sort="Ameen, Mohammed S" uniqKey="Ameen M" first="Mohammed S." last="Ameen">Mohammed S. Ameen</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction</title>
<author><name sortKey="Al Ayed, Mohamed S" sort="Al Ayed, Mohamed S" uniqKey="Al Ayed M" first="Mohamed S." last="Al-Ayed">Mohamed S. Al-Ayed</name>
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<author><name sortKey="Asaad, Ahmed M" sort="Asaad, Ahmed M" uniqKey="Asaad A" first="Ahmed M." last="Asaad">Ahmed M. Asaad</name>
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<author><name sortKey="Qureshi, Mohamed A" sort="Qureshi, Mohamed A" uniqKey="Qureshi M" first="Mohamed A." last="Qureshi">Mohamed A. Qureshi</name>
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<author><name sortKey="Ameen, Mohammed S" sort="Ameen, Mohammed S" uniqKey="Ameen M" first="Mohammed S." last="Ameen">Mohammed S. Ameen</name>
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<series><title level="j">Saudi Medical Journal</title>
<idno type="ISSN">0379-5284</idno>
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<front><div type="abstract" xml:lang="en"><sec id="st1"><title>Objectives:</title>
<p>To investigate 15 respiratory viruses in children with acute respiratory tract infections (ARTIs) using multiplex reverse-transcriptase polymerase chain reaction (RT-PCR), and to analyze the clinical and epidemiological features of these viruses.</p>
</sec>
<sec id="st2"><title>Methods:</title>
<p>In a cross-sectional study, 135 children, ≤5 years of age who presented with ARTIs in Najran Maternity and Children Hospital, Najran, Saudi Arabia between October 2012 and July 2013 were included. The clinical and sociodemographic data, and the laboratory results were recorded using a standardized questionnaire. Two nasopharyngeal swabs were collected from each child: one for bacteriological examination, and the second for viral detection using multiplex RT-PCR.</p>
</sec>
<sec id="st3"><title>Results:</title>
<p>A single viral pathogen was detected in 76 patients, viral coinfections in 9, and mixed viral and bacterial pathogens in 15. Respiratory syncytial virus was isolated in 33 patients, human rhinovirus (hRV) in 22, adenovirus (AdV) in 19, human metapneumovirus in 13, influenza virus in 10, parainfluenza virus in 7, human corona virus (hCoV) in 4, and human bocavirus in one.</p>
</sec>
<sec id="st4"><title>Conclusion</title>
<p>Respiratory syncytial virus, hRV, and AdV were the most frequent viruses, accounting for more than two-thirds of the cases. Other viruses, such as MPV, hCoV NL63, and hCoV OC43, may play a role in pediatric ARTIs. Of significance is the potential use of multiplex RT-PCR to provide epidemiological and virological data for early detection of the emergence of novel respiratory viruses in the era of the Middle East respiratory syndrome coronavirus.</p>
</sec>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Saudi Med J</journal-id>
<journal-id journal-id-type="iso-abbrev">Saudi Med J</journal-id>
<journal-id journal-id-type="publisher-id">SaudiMedJ</journal-id>
<journal-title-group><journal-title>Saudi Medical Journal</journal-title>
</journal-title-group>
<issn pub-type="ppub">0379-5284</issn>
<issn pub-type="epub">1658-3175</issn>
<publisher><publisher-name>Saudi Medical Journal</publisher-name>
<publisher-loc>Saudi Arabia</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">25399211</article-id>
<article-id pub-id-type="pmc">4362149</article-id>
<article-id pub-id-type="publisher-id">SaudiMedJ-35-1348</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
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</article-categories>
<title-group><article-title>Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Al-Ayed</surname>
<given-names>Mohamed S.</given-names>
</name>
<degrees>MBBS, MD</degrees>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Asaad</surname>
<given-names>Ahmed M.</given-names>
</name>
<degrees>MBBS, MD</degrees>
<xref ref-type="aff" rid="aff1"></xref>
<xref ref-type="corresp" rid="cor1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Qureshi</surname>
<given-names>Mohamed A.</given-names>
</name>
<degrees>BSc, MSc</degrees>
</contrib>
<contrib contrib-type="author"><name><surname>Ameen</surname>
<given-names>Mohammed S.</given-names>
</name>
<degrees>MBBS, MD</degrees>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
</contrib-group>
<aff id="aff1"><italic>From the Departments of Pediatrics (Al-Ayed, Ameen), Microbiology (Assad, Qureshi) College of Medicine, Najran University, Najran, Kingdom of Saudi Arabia.</italic>
</aff>
<author-notes><corresp id="cor1"><italic>Address correspondence and reprint request to: Dr. Ahmed M. Asaad, Professor of Microbiology, College of Medicine, Najran University, PO Box 1988, Najran, Kingdom of Saudi Arabia. Tel. +966 (17) 5428516. Fax. +966 (17) 5442419. E-mail: <email xlink:href="ahmedmoradasaad@hotmail.com">ahmedmoradasaad@hotmail.com</email>
/<email xlink:href="amasad@nu.edu.sa">amasad@nu.edu.sa</email>
</italic>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><year>2014</year>
</pub-date>
<volume>35</volume>
<issue>11</issue>
<fpage>1348</fpage>
<lpage>1353</lpage>
<history><date date-type="received"><day>29</day>
<month>5</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>19</day>
<month>8</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>Copyright: © Saudi Medical Journal</copyright-statement>
<copyright-year>2014</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0"><license-p>Saudi Medical Journal is an Open Access journal and articles published are distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC). Readers may copy, distribute, and display the work for non-commercial purposes with the proper citation of the original work.</license-p>
</license>
</permissions>
<abstract><sec id="st1"><title>Objectives:</title>
<p>To investigate 15 respiratory viruses in children with acute respiratory tract infections (ARTIs) using multiplex reverse-transcriptase polymerase chain reaction (RT-PCR), and to analyze the clinical and epidemiological features of these viruses.</p>
</sec>
<sec id="st2"><title>Methods:</title>
<p>In a cross-sectional study, 135 children, ≤5 years of age who presented with ARTIs in Najran Maternity and Children Hospital, Najran, Saudi Arabia between October 2012 and July 2013 were included. The clinical and sociodemographic data, and the laboratory results were recorded using a standardized questionnaire. Two nasopharyngeal swabs were collected from each child: one for bacteriological examination, and the second for viral detection using multiplex RT-PCR.</p>
</sec>
<sec id="st3"><title>Results:</title>
<p>A single viral pathogen was detected in 76 patients, viral coinfections in 9, and mixed viral and bacterial pathogens in 15. Respiratory syncytial virus was isolated in 33 patients, human rhinovirus (hRV) in 22, adenovirus (AdV) in 19, human metapneumovirus in 13, influenza virus in 10, parainfluenza virus in 7, human corona virus (hCoV) in 4, and human bocavirus in one.</p>
</sec>
<sec id="st4"><title>Conclusion</title>
<p>Respiratory syncytial virus, hRV, and AdV were the most frequent viruses, accounting for more than two-thirds of the cases. Other viruses, such as MPV, hCoV NL63, and hCoV OC43, may play a role in pediatric ARTIs. Of significance is the potential use of multiplex RT-PCR to provide epidemiological and virological data for early detection of the emergence of novel respiratory viruses in the era of the Middle East respiratory syndrome coronavirus.</p>
</sec>
</abstract>
</article-meta>
</front>
</pmc>
</record>
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