Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
Identifieur interne : 000844 ( Pmc/Curation ); précédent : 000843; suivant : 000845Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
Auteurs : Regina Konrad [Allemagne] ; Ute Eberle [Allemagne] ; Alexandra Dangel [Allemagne] ; Bianca Treis [Allemagne] ; Anja Berger [Allemagne] ; Katja Bengs [Allemagne] ; Volker Fingerle [Allemagne] ; Bernhard Liebl [Allemagne] ; Nikolaus Ackermann [Allemagne] ; Andreas Sing [Allemagne]Source :
- Eurosurveillance [ 1025-496X ] ; 2020.
Abstract
The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.
Url:
DOI: 10.2807/1560-7917.ES.2020.25.9.2000173
PubMed: 32156330
PubMed Central: 7068163
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000844
Links to Exploration step
PMC:7068163Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020</title><author><name sortKey="Konrad, Regina" sort="Konrad, Regina" uniqKey="Konrad R" first="Regina" last="Konrad">Regina Konrad</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation></author><author><name sortKey="Eberle, Ute" sort="Eberle, Ute" uniqKey="Eberle U" first="Ute" last="Eberle">Ute Eberle</name><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation></author><author><name sortKey="Dangel, Alexandra" sort="Dangel, Alexandra" uniqKey="Dangel A" first="Alexandra" last="Dangel">Alexandra Dangel</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation></author><author><name sortKey="Treis, Bianca" sort="Treis, Bianca" uniqKey="Treis B" first="Bianca" last="Treis">Bianca Treis</name><affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation></author><author><name sortKey="Berger, Anja" sort="Berger, Anja" uniqKey="Berger A" first="Anja" last="Berger">Anja Berger</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation></author><author><name sortKey="Bengs, Katja" sort="Bengs, Katja" uniqKey="Bengs K" first="Katja" last="Bengs">Katja Bengs</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation></author><author><name sortKey="Fingerle, Volker" sort="Fingerle, Volker" uniqKey="Fingerle V" first="Volker" last="Fingerle">Volker Fingerle</name><affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation></author><author><name sortKey="Liebl, Bernhard" sort="Liebl, Bernhard" uniqKey="Liebl B" first="Bernhard" last="Liebl">Bernhard Liebl</name><affiliation wicri:level="1"><nlm:aff id="aff4">State Institute of Health, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>State Institute of Health, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5">Ludwig Maximilians-Universität, Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ludwig Maximilians-Universität, Munich</wicri:regionArea></affiliation></author><author><name sortKey="Ackermann, Nikolaus" sort="Ackermann, Nikolaus" uniqKey="Ackermann N" first="Nikolaus" last="Ackermann">Nikolaus Ackermann</name><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation></author><author><name sortKey="Sing, Andreas" sort="Sing, Andreas" uniqKey="Sing A" first="Andreas" last="Sing">Andreas Sing</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5">Ludwig Maximilians-Universität, Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ludwig Maximilians-Universität, Munich</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">32156330</idno><idno type="pmc">7068163</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068163</idno><idno type="RBID">PMC:7068163</idno><idno type="doi">10.2807/1560-7917.ES.2020.25.9.2000173</idno><date when="2020">2020</date><idno type="wicri:Area/Pmc/Corpus">000844</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000844</idno><idno type="wicri:Area/Pmc/Curation">000844</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000844</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020</title><author><name sortKey="Konrad, Regina" sort="Konrad, Regina" uniqKey="Konrad R" first="Regina" last="Konrad">Regina Konrad</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation></author><author><name sortKey="Eberle, Ute" sort="Eberle, Ute" uniqKey="Eberle U" first="Ute" last="Eberle">Ute Eberle</name><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation></author><author><name sortKey="Dangel, Alexandra" sort="Dangel, Alexandra" uniqKey="Dangel A" first="Alexandra" last="Dangel">Alexandra Dangel</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation></author><author><name sortKey="Treis, Bianca" sort="Treis, Bianca" uniqKey="Treis B" first="Bianca" last="Treis">Bianca Treis</name><affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation></author><author><name sortKey="Berger, Anja" sort="Berger, Anja" uniqKey="Berger A" first="Anja" last="Berger">Anja Berger</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation></author><author><name sortKey="Bengs, Katja" sort="Bengs, Katja" uniqKey="Bengs K" first="Katja" last="Bengs">Katja Bengs</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation></author><author><name sortKey="Fingerle, Volker" sort="Fingerle, Volker" uniqKey="Fingerle V" first="Volker" last="Fingerle">Volker Fingerle</name><affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation></author><author><name sortKey="Liebl, Bernhard" sort="Liebl, Bernhard" uniqKey="Liebl B" first="Bernhard" last="Liebl">Bernhard Liebl</name><affiliation wicri:level="1"><nlm:aff id="aff4">State Institute of Health, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>State Institute of Health, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5">Ludwig Maximilians-Universität, Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ludwig Maximilians-Universität, Munich</wicri:regionArea></affiliation></author><author><name sortKey="Ackermann, Nikolaus" sort="Ackermann, Nikolaus" uniqKey="Ackermann N" first="Nikolaus" last="Ackermann">Nikolaus Ackermann</name><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation></author><author><name sortKey="Sing, Andreas" sort="Sing, Andreas" uniqKey="Sing A" first="Andreas" last="Sing">Andreas Sing</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea></affiliation><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5">Ludwig Maximilians-Universität, Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ludwig Maximilians-Universität, Munich</wicri:regionArea></affiliation></author></analytic><series><title level="j">Eurosurveillance</title><idno type="ISSN">1025-496X</idno><idno type="eISSN">1560-7917</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well. </p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Rothe, C" uniqKey="Rothe C">C Rothe</name></author><author><name sortKey="Schunk, M" uniqKey="Schunk M">M Schunk</name></author><author><name sortKey="Sothmann, P" uniqKey="Sothmann P">P Sothmann</name></author><author><name sortKey="Bretzel, G" uniqKey="Bretzel G">G Bretzel</name></author><author><name sortKey="Froeschl, G" uniqKey="Froeschl G">G Froeschl</name></author><author><name sortKey="Wallrauch, C" uniqKey="Wallrauch C">C Wallrauch</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O Landt</name></author><author><name sortKey="Kaiser, M" uniqKey="Kaiser M">M Kaiser</name></author><author><name sortKey="Molenkamp, R" uniqKey="Molenkamp R">R Molenkamp</name></author><author><name sortKey="Meijer, A" uniqKey="Meijer A">A Meijer</name></author><author><name sortKey="Chu, Dkw" uniqKey="Chu D">DKW Chu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Romette, Jl" uniqKey="Romette J">JL Romette</name></author><author><name sortKey="Prat, Cm" uniqKey="Prat C">CM Prat</name></author><author><name sortKey="Gould, Ea" uniqKey="Gould E">EA Gould</name></author><author><name sortKey="De Lamballerie, X" uniqKey="De Lamballerie X">X de Lamballerie</name></author><author><name sortKey="Charrel, R" uniqKey="Charrel R">R Charrel</name></author><author><name sortKey="Coutard, B" uniqKey="Coutard B">B Coutard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hummel, Kb" uniqKey="Hummel K">KB Hummel</name></author><author><name sortKey="Lowe, L" uniqKey="Lowe L">L Lowe</name></author><author><name sortKey="Bellini, Wj" uniqKey="Bellini W">WJ Bellini</name></author><author><name sortKey="Rota, Pa" uniqKey="Rota P">PA Rota</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Ren, Ll" uniqKey="Ren L">LL Ren</name></author><author><name sortKey="Wang, Ym" uniqKey="Wang Y">YM Wang</name></author><author><name sortKey="Wu, Zq" uniqKey="Wu Z">ZQ Wu</name></author><author><name sortKey="Xiang, Zc" uniqKey="Xiang Z">ZC Xiang</name></author><author><name sortKey="Guo, L" uniqKey="Guo L">L Guo</name></author><author><name sortKey="Xu, T" uniqKey="Xu T">T Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, F" uniqKey="Wu F">F Wu</name></author><author><name sortKey="Zhao, S" uniqKey="Zhao S">S Zhao</name></author><author><name sortKey="Yu, B" uniqKey="Yu B">B Yu</name></author><author><name sortKey="Chen, Ym" uniqKey="Chen Y">YM Chen</name></author><author><name sortKey="Wang, W" uniqKey="Wang W">W Wang</name></author><author><name sortKey="Song, Zg" uniqKey="Song Z">ZG Song</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reusken, Cbem" uniqKey="Reusken C">CBEM Reusken</name></author><author><name sortKey="Broberg, Ek" uniqKey="Broberg E">EK Broberg</name></author><author><name sortKey="Haagmans, B" uniqKey="Haagmans B">B Haagmans</name></author><author><name sortKey="Meijer, A" uniqKey="Meijer A">A Meijer</name></author><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Papa, A" uniqKey="Papa A">A Papa</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Euro Surveill</journal-id><journal-id journal-id-type="iso-abbrev">Euro Surveill</journal-id><journal-id journal-id-type="publisher-id">ES</journal-id><journal-title-group><journal-title>Eurosurveillance</journal-title></journal-title-group><issn pub-type="ppub">1025-496X</issn><issn pub-type="epub">1560-7917</issn><publisher><publisher-name>European Centre for Disease Prevention and Control (ECDC)</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">32156330</article-id><article-id pub-id-type="pmc">7068163</article-id><article-id pub-id-type="publisher-id">2000173</article-id><article-id pub-id-type="doi">10.2807/1560-7917.ES.2020.25.9.2000173</article-id><article-id pub-id-type="publisher-id">2000173</article-id><article-categories><subj-group subj-group-type="heading"><subject>Rapid Communication</subject></subj-group></article-categories><title-group><article-title>Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Konrad</surname><given-names>Regina</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Eberle</surname><given-names>Ute</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0001-6737-5681</contrib-id><name><surname>Dangel</surname><given-names>Alexandra</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Treis</surname><given-names>Bianca</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Berger</surname><given-names>Anja</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Bengs</surname><given-names>Katja</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Fingerle</surname><given-names>Volker</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Liebl</surname><given-names>Bernhard</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ackermann</surname><given-names>Nikolaus</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sing</surname><given-names>Andreas</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><aff id="aff1"><label>1</label>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</aff><aff id="aff2"><label>2</label>These authors contributed equally to this work</aff><aff id="aff3"><label>3</label>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</aff><aff id="aff4"><label>4</label>State Institute of Health, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</aff><aff id="aff5"><label>5</label>Ludwig Maximilians-Universität, Munich, Germany</aff></contrib-group><author-notes><fn id="afn1"><p>Correspondence: Alexandra Dangel (<email xlink:href="alexandra.dangel@lgl.bayern.de">alexandra.dangel@lgl.bayern.de</email>)</p></fn></author-notes><pub-date pub-type="ppub"><day>05</day><month>3</month><year>2020</year></pub-date><volume>25</volume><issue>9</issue><elocation-id>2000173</elocation-id><history><date date-type="received"><day>21</day><month>2</month><year>2020</year></date><date date-type="accepted"><day>04</day><month>3</month><year>2020</year></date></history><permissions><copyright-statement>This article is copyright of the authors or their affiliated institutions, 2020.</copyright-statement><copyright-year>2020</copyright-year><copyright-holder>The authors or their affiliated institutions</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.</license-p></license></permissions><abstract><p>The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well. </p></abstract><kwd-group><title>Keywords: </title><kwd>SARS-CoV-2</kwd><kwd>Molecular Testing</kwd><kwd>Laboratory Diagnostics</kwd><kwd>Real-Time PCR</kwd><kwd>COVID-19</kwd><kwd>Corona Virus</kwd><kwd>Infectious disease</kwd><kwd>outbreaks</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000844 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000844 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:7068163
|texte= Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:32156330" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a CovidV2
| This area was generated with Dilib version V0.6.33. |  |