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Label-free microcantilever-based immunosensors for highly sensitive determination of avian influenza virus H9

Identifieur interne : 000301 ( Pmc/Curation ); précédent : 000300; suivant : 000302

Label-free microcantilever-based immunosensors for highly sensitive determination of avian influenza virus H9

Auteurs : Dan Xu [République populaire de Chine] ; Lin Liu [République populaire de Chine] ; Jun Guan [République populaire de Chine] ; Jianyun Xu [République populaire de Chine] ; Ting Wang [République populaire de Chine] ; Aijian Qin [République populaire de Chine] ; Xiaoya Hu [République populaire de Chine] ; Chengyin Wang [République populaire de Chine]

Source :

RBID : PMC:7087674

Abstract

We report on label-free immunosensors for the highly sensitive detection of avian influenza virus. The method makes use of the microcantilevers of an atomic force microscope onto which monoclonal antibodies against avian influenza virus were covalently immobilized. The factors influencing the performance of the resulting immunosensors were optimized by measuring the deflections of the cantilever via optical reflection, and this resulted in low detection limits and a wide analytical range. The differential deflection signals revealed specific antigen binding and their intensity is proportional to the logarithm of the concentrations of the virus in solution. Under optimal conditions, the immunosensors exhibit a linear response in the 7.6 ng mL−1 to 76 μg mL−1 concentration range of avian influenza virus, and the detection limit is 1.9 ng mL−1.

Label-free immunosensors based on microcantilevers of an atomic force microscope was fabricated by covalently immobilizing monoclonal antibodies to avian influenza virus onto the microcantilever. The performance and factors influencing the performance of the resulting immunosensors were investigated in detail by measuring the cantilever deflections using the optical reflection technique.

Electronic supplementary material

The online version of this article (doi:10.1007/s00604-013-1129-x) contains supplementary material, which is available to authorized users.


Url:
DOI: 10.1007/s00604-013-1129-x
PubMed: NONE
PubMed Central: 7087674

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PMC:7087674

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<p>We report on label-free immunosensors for the highly sensitive detection of avian influenza virus. The method makes use of the microcantilevers of an atomic force microscope onto which monoclonal antibodies against avian influenza virus were covalently immobilized. The factors influencing the performance of the resulting immunosensors were optimized by measuring the deflections of the cantilever via optical reflection, and this resulted in low detection limits and a wide analytical range. The differential deflection signals revealed specific antigen binding and their intensity is proportional to the logarithm of the concentrations of the virus in solution. Under optimal conditions, the immunosensors exhibit a linear response in the 7.6 ng mL
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<pmc article-type="research-article">
<pmc-dir>properties open_access</pmc-dir>
<front>
<journal-meta>
<journal-id journal-id-type="nlm-ta">Mikrochim Acta</journal-id>
<journal-id journal-id-type="iso-abbrev">Mikrochim Acta</journal-id>
<journal-title-group>
<journal-title>Mikrochimica Acta</journal-title>
</journal-title-group>
<issn pub-type="ppub">0026-3672</issn>
<issn pub-type="epub">1436-5073</issn>
<publisher>
<publisher-name>Springer Vienna</publisher-name>
<publisher-loc>Vienna</publisher-loc>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="pmc">7087674</article-id>
<article-id pub-id-type="publisher-id">1129</article-id>
<article-id pub-id-type="doi">10.1007/s00604-013-1129-x</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Original Paper</subject>
</subj-group>
</article-categories>
<title-group>
<article-title>Label-free microcantilever-based immunosensors for highly sensitive determination of avian influenza virus H9</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname>Xu</surname>
<given-names>Dan</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Liu</surname>
<given-names>Lin</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Guan</surname>
<given-names>Jun</given-names>
</name>
<xref ref-type="aff" rid="Aff4">4</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Xu</surname>
<given-names>Jianyun</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Wang</surname>
<given-names>Ting</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Qin</surname>
<given-names>Aijian</given-names>
</name>
<xref ref-type="aff" rid="Aff3">3</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Hu</surname>
<given-names>Xiaoya</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author" corresp="yes">
<name>
<surname>Wang</surname>
<given-names>Chengyin</given-names>
</name>
<address>
<phone>+86-514-87888454</phone>
<fax>+86-514-87975244</fax>
<email>wangcy@yzu.edu.cn</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<aff id="Aff1">
<label>1</label>
<institution-wrap>
<institution-id institution-id-type="GRID">grid.268415.c</institution-id>
<institution>College of Chemistry and Chemical Engineering,</institution>
<institution>Yangzhou University,</institution>
</institution-wrap>
Yangzhou, 225002 China</aff>
<aff id="Aff2">
<label>2</label>
Jiangsu Key Laboratory of Environmental Engineering and Monitoring, Yangzhou, 225002 China</aff>
<aff id="Aff3">
<label>3</label>
<institution-wrap>
<institution-id institution-id-type="GRID">grid.268415.c</institution-id>
<institution>College of Veterinary Medicine,</institution>
<institution>Yangzhou University,</institution>
</institution-wrap>
Yangzhou, 225002 China</aff>
<aff id="Aff4">
<label>4</label>
<institution-wrap>
<institution-id institution-id-type="GRID">grid.452743.3</institution-id>
<institution-id institution-id-type="ISNI">0000000417884869</institution-id>
<institution>Clinical College of Yangzhou University, Subei People’s Hospital of Jiangsu Province,</institution>
</institution-wrap>
Yangzhou, 225002 China</aff>
</contrib-group>
<pub-date pub-type="epub">
<day>21</day>
<month>11</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="ppub">
<year>2014</year>
</pub-date>
<volume>181</volume>
<issue>3</issue>
<fpage>403</fpage>
<lpage>410</lpage>
<history>
<date date-type="received">
<day>18</day>
<month>7</month>
<year>2013</year>
</date>
<date date-type="accepted">
<day>3</day>
<month>11</month>
<year>2013</year>
</date>
</history>
<permissions>
<copyright-statement>© Springer-Verlag Wien 2013</copyright-statement>
<license>
<license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p>
</license>
</permissions>
<abstract id="Abs1">
<p>We report on label-free immunosensors for the highly sensitive detection of avian influenza virus. The method makes use of the microcantilevers of an atomic force microscope onto which monoclonal antibodies against avian influenza virus were covalently immobilized. The factors influencing the performance of the resulting immunosensors were optimized by measuring the deflections of the cantilever via optical reflection, and this resulted in low detection limits and a wide analytical range. The differential deflection signals revealed specific antigen binding and their intensity is proportional to the logarithm of the concentrations of the virus in solution. Under optimal conditions, the immunosensors exhibit a linear response in the 7.6 ng mL
<sup>−1</sup>
to 76 μg mL
<sup>−1</sup>
concentration range of avian influenza virus, and the detection limit is 1.9 ng mL
<sup>−1</sup>
.</p>
<p>
<fig position="anchor" id="Figa">
<label>Figure</label>
<caption>
<p>Label-free immunosensors based on microcantilevers of an atomic force microscope was fabricated by covalently immobilizing monoclonal antibodies to avian influenza virus onto the microcantilever. The performance and factors influencing the performance of the resulting immunosensors were investigated in detail by measuring the cantilever deflections using the optical reflection technique.</p>
</caption>
<graphic position="anchor" xlink:href="604_2013_1129_Figa_HTML" id="MO1"></graphic>
</fig>
</p>
<sec>
<title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1007/s00604-013-1129-x) contains supplementary material, which is available to authorized users.</p>
</sec>
</abstract>
<kwd-group xml:lang="en">
<title>Keywords</title>
<kwd>Avian influenza virus</kwd>
<kwd>Label-free</kwd>
<kwd>Immunosensor</kwd>
<kwd>Microcantilever</kwd>
<kwd>Atomic force microscope</kwd>
</kwd-group>
<custom-meta-group>
<custom-meta>
<meta-name>issue-copyright-statement</meta-name>
<meta-value>© Springer-Verlag Wien 2014</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
</record>

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