Surface-displayed porcine reproductive and respiratory syndrome virus from cell culture onto gram-positive enhancer matrix particles
Identifieur interne : 000275 ( Pmc/Curation ); précédent : 000274; suivant : 000276Surface-displayed porcine reproductive and respiratory syndrome virus from cell culture onto gram-positive enhancer matrix particles
Auteurs : Lan Li ; Xuwen Qiao ; Jin Chen ; Yuanpeng Zhang ; Qisheng Zheng ; Jibo HouSource :
- Journal of Industrial Microbiology & Biotechnology [ 1367-5435 ] ; 2018.
Abstract
Vaccine immunization is now one of the most effective ways to control porcine reproductive and respiratory syndrome virus (PRRSV) infection. Impurity is one of the main factors affecting vaccine safety and efficacy. Here we present a novel innovative PRRSV purification approach based on surface display technology. First, a bifunctional protein PA-GRFT (protein anchor-griffithsin), the crucial factor in the purification process, was successfully produced in
Url:
DOI: 10.1007/s10295-018-2061-1
PubMed: 30046953
PubMed Central: 7088258
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000275
Links to Exploration step
PMC:7088258Curation
No country items
Lan Li<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff2">Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">225009 Jiangsu China</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff2">Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">225009 Jiangsu China</wicri:noCountry>
</affiliation>
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Surface-displayed porcine reproductive and respiratory syndrome virus from cell culture onto gram-positive enhancer matrix particles</title>
<author><name sortKey="Li, Lan" sort="Li, Lan" uniqKey="Li L" first="Lan" last="Li">Lan Li</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Qiao, Xuwen" sort="Qiao, Xuwen" uniqKey="Qiao X" first="Xuwen" last="Qiao">Xuwen Qiao</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Chen, Jin" sort="Chen, Jin" uniqKey="Chen J" first="Jin" last="Chen">Jin Chen</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Zhang, Yuanpeng" sort="Zhang, Yuanpeng" uniqKey="Zhang Y" first="Yuanpeng" last="Zhang">Yuanpeng Zhang</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Zheng, Qisheng" sort="Zheng, Qisheng" uniqKey="Zheng Q" first="Qisheng" last="Zheng">Qisheng Zheng</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Hou, Jibo" sort="Hou, Jibo" uniqKey="Hou J" first="Jibo" last="Hou">Jibo Hou</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff2">Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">225009 Jiangsu China</wicri:noCountry>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">30046953</idno>
<idno type="pmc">7088258</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088258</idno>
<idno type="RBID">PMC:7088258</idno>
<idno type="doi">10.1007/s10295-018-2061-1</idno>
<date when="2018">2018</date>
<idno type="wicri:Area/Pmc/Corpus">000275</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000275</idno>
<idno type="wicri:Area/Pmc/Curation">000275</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000275</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Surface-displayed porcine reproductive and respiratory syndrome virus from cell culture onto gram-positive enhancer matrix particles</title>
<author><name sortKey="Li, Lan" sort="Li, Lan" uniqKey="Li L" first="Lan" last="Li">Lan Li</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Qiao, Xuwen" sort="Qiao, Xuwen" uniqKey="Qiao X" first="Xuwen" last="Qiao">Xuwen Qiao</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Chen, Jin" sort="Chen, Jin" uniqKey="Chen J" first="Jin" last="Chen">Jin Chen</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Zhang, Yuanpeng" sort="Zhang, Yuanpeng" uniqKey="Zhang Y" first="Yuanpeng" last="Zhang">Yuanpeng Zhang</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Zheng, Qisheng" sort="Zheng, Qisheng" uniqKey="Zheng Q" first="Qisheng" last="Zheng">Qisheng Zheng</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Hou, Jibo" sort="Hou, Jibo" uniqKey="Hou J" first="Jibo" last="Hou">Jibo Hou</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry>
</affiliation>
<affiliation><nlm:aff id="Aff2">Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009 Jiangsu China</nlm:aff>
<wicri:noCountry code="subfield">225009 Jiangsu China</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Industrial Microbiology & Biotechnology</title>
<idno type="ISSN">1367-5435</idno>
<idno type="eISSN">1476-5535</idno>
<imprint><date when="2018">2018</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="Par1">Vaccine immunization is now one of the most effective ways to control porcine reproductive and respiratory syndrome virus (PRRSV) infection. Impurity is one of the main factors affecting vaccine safety and efficacy. Here we present a novel innovative PRRSV purification approach based on surface display technology. First, a bifunctional protein PA-GRFT (protein anchor-griffithsin), the crucial factor in the purification process, was successfully produced in <italic>Escherichia coli</italic>
yielding 80 mg/L of broth culture. Then PRRSV purification was performed by incubation of PA-GRFT with PRRSV and gram-positive enhancer matrix (GEM) particles, followed by centrifugation to collect virions loaded onto GEM particles. Our results showed that most of the bulk impurities had been removed, and PA-GRFT could capture PRRSV onto GEM particles. Our lactic acid bacteria-based purification method, which is promising as ease of operation, low cost and easy to scale-up, may represent a candidate method for the large-scale purification of this virus for vaccine production.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Bohua, L" uniqKey="Bohua L">L Bohua</name>
</author>
<author><name sortKey="Ming, S" uniqKey="Ming S">S Ming</name>
</author>
<author><name sortKey="Lu, Y" uniqKey="Lu Y">Y Lu</name>
</author>
<author><name sortKey="Xiaoyu, D" uniqKey="Xiaoyu D">D Xiaoyu</name>
</author>
<author><name sortKey="Baochun, L" uniqKey="Baochun L">L Baochun</name>
</author>
<author><name sortKey="Fenqin, S" uniqKey="Fenqin S">S Fenqin</name>
</author>
<author><name sortKey="Li, Z" uniqKey="Li Z">Z Li</name>
</author>
<author><name sortKey="Xizhao, C" uniqKey="Xizhao C">C Xizhao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bosma, T" uniqKey="Bosma T">T Bosma</name>
</author>
<author><name sortKey="Kanninga, R" uniqKey="Kanninga R">R Kanninga</name>
</author>
<author><name sortKey="Neef, J" uniqKey="Neef J">J Neef</name>
</author>
<author><name sortKey="Audouy, Sa" uniqKey="Audouy S">SA Audouy</name>
</author>
<author><name sortKey="Van Roosmalen, Ml" uniqKey="Van Roosmalen M">ML van Roosmalen</name>
</author>
<author><name sortKey="Steen, A" uniqKey="Steen A">A Steen</name>
</author>
<author><name sortKey="Buist, G" uniqKey="Buist G">G Buist</name>
</author>
<author><name sortKey="Kok, J" uniqKey="Kok J">J Kok</name>
</author>
<author><name sortKey="Kuipers, Op" uniqKey="Kuipers O">OP Kuipers</name>
</author>
<author><name sortKey="Robillard, G" uniqKey="Robillard G">G Robillard</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Buist, G" uniqKey="Buist G">G Buist</name>
</author>
<author><name sortKey="Steen, A" uniqKey="Steen A">A Steen</name>
</author>
<author><name sortKey="Kok, J" uniqKey="Kok J">J Kok</name>
</author>
<author><name sortKey="Kuipers, Op" uniqKey="Kuipers O">OP Kuipers</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chand, Rj" uniqKey="Chand R">RJ Chand</name>
</author>
<author><name sortKey="Trible, Br" uniqKey="Trible B">BR Trible</name>
</author>
<author><name sortKey="Rowland, Rr" uniqKey="Rowland R">RR Rowland</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fuqua, Jl" uniqKey="Fuqua J">JL Fuqua</name>
</author>
<author><name sortKey="Wanga, V" uniqKey="Wanga V">V Wanga</name>
</author>
<author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Giomarelli, B" uniqKey="Giomarelli B">B Giomarelli</name>
</author>
<author><name sortKey="Schumacher, Km" uniqKey="Schumacher K">KM Schumacher</name>
</author>
<author><name sortKey="Taylor, Te" uniqKey="Taylor T">TE Taylor</name>
</author>
<author><name sortKey="Hartley, Jl" uniqKey="Hartley J">JL Hartley</name>
</author>
<author><name sortKey="Mcmahon, Jb" uniqKey="Mcmahon J">JB Mcmahon</name>
</author>
<author><name sortKey="Mori, T" uniqKey="Mori T">T Mori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hou, Xl" uniqKey="Hou X">XL Hou</name>
</author>
<author><name sortKey="Yu, Ly" uniqKey="Yu L">LY Yu</name>
</author>
<author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name>
</author>
<author><name sortKey="Wang, Gh" uniqKey="Wang G">GH Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hu, J" uniqKey="Hu J">J Hu</name>
</author>
<author><name sortKey="Ni, Y" uniqKey="Ni Y">Y Ni</name>
</author>
<author><name sortKey="Dryman, Ba" uniqKey="Dryman B">BA Dryman</name>
</author>
<author><name sortKey="Meng, Xj" uniqKey="Meng X">XJ Meng</name>
</author>
<author><name sortKey="Zhang, C" uniqKey="Zhang C">C Zhang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ishag, Hz" uniqKey="Ishag H">HZ Ishag</name>
</author>
<author><name sortKey="Li, C" uniqKey="Li C">C Li</name>
</author>
<author><name sortKey="Wang, F" uniqKey="Wang F">F Wang</name>
</author>
<author><name sortKey="Mao, X" uniqKey="Mao X">X Mao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kouokam, Jc" uniqKey="Kouokam J">JC Kouokam</name>
</author>
<author><name sortKey="Huskens, D" uniqKey="Huskens D">D Huskens</name>
</author>
<author><name sortKey="Schols, D" uniqKey="Schols D">D Schols</name>
</author>
<author><name sortKey="Johannemann, A" uniqKey="Johannemann A">A Johannemann</name>
</author>
<author><name sortKey="Riedell, Sk" uniqKey="Riedell S">SK Riedell</name>
</author>
<author><name sortKey="Walter, W" uniqKey="Walter W">W Walter</name>
</author>
<author><name sortKey="Walker, Jm" uniqKey="Walker J">JM Walker</name>
</author>
<author><name sortKey="Matoba, N" uniqKey="Matoba N">N Matoba</name>
</author>
<author><name sortKey="O Eefe, Br" uniqKey="O Eefe B">BR O’Keefe</name>
</author>
<author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kouokam, Jc" uniqKey="Kouokam J">JC Kouokam</name>
</author>
<author><name sortKey="Lasnik, Ab" uniqKey="Lasnik A">AB Lasnik</name>
</author>
<author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lan, L" uniqKey="Lan L">L Lan</name>
</author>
<author><name sortKey="Zheng, Q" uniqKey="Zheng Q">Q Zheng</name>
</author>
<author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name>
</author>
<author><name sortKey="Li, P" uniqKey="Li P">P Li</name>
</author>
<author><name sortKey="Fu, Y" uniqKey="Fu Y">Y Fu</name>
</author>
<author><name sortKey="Hou, J" uniqKey="Hou J">J Hou</name>
</author>
<author><name sortKey="Xiao, X" uniqKey="Xiao X">X Xiao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Levendosky, K" uniqKey="Levendosky K">K Levendosky</name>
</author>
<author><name sortKey="Mizenina, O" uniqKey="Mizenina O">O Mizenina</name>
</author>
<author><name sortKey="Martinelli, E" uniqKey="Martinelli E">E Martinelli</name>
</author>
<author><name sortKey="Jeanpierre, N" uniqKey="Jeanpierre N">N Jeanpierre</name>
</author>
<author><name sortKey="Kizima, L" uniqKey="Kizima L">L Kizima</name>
</author>
<author><name sortKey="Rodriguez, A" uniqKey="Rodriguez A">A Rodriguez</name>
</author>
<author><name sortKey="Kleinbeck, K" uniqKey="Kleinbeck K">K Kleinbeck</name>
</author>
<author><name sortKey="Bonnaire, T" uniqKey="Bonnaire T">T Bonnaire</name>
</author>
<author><name sortKey="Robbiani, M" uniqKey="Robbiani M">M Robbiani</name>
</author>
<author><name sortKey="Zydowsky, Tm" uniqKey="Zydowsky T">TM Zydowsky</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J Li</name>
</author>
<author><name sortKey="Tao, S" uniqKey="Tao S">S Tao</name>
</author>
<author><name sortKey="Orlando, R" uniqKey="Orlando R">R Orlando</name>
</author>
<author><name sortKey="Murtaugh, Mp" uniqKey="Murtaugh M">MP Murtaugh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, Pc" uniqKey="Li P">PC Li</name>
</author>
<author><name sortKey="Qiao, Xw" uniqKey="Qiao X">XW Qiao</name>
</author>
<author><name sortKey="Zheng, Qs" uniqKey="Zheng Q">QS Zheng</name>
</author>
<author><name sortKey="Hou, Jb" uniqKey="Hou J">JB Hou</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lusvarghi, S" uniqKey="Lusvarghi S">S Lusvarghi</name>
</author>
<author><name sortKey="Bewley, Ca" uniqKey="Bewley C">CA Bewley</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lyoo, Ys" uniqKey="Lyoo Y">YS Lyoo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mao, R" uniqKey="Mao R">R Mao</name>
</author>
<author><name sortKey="Zhou, K" uniqKey="Zhou K">K Zhou</name>
</author>
<author><name sortKey="Han, Z" uniqKey="Han Z">Z Han</name>
</author>
<author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Matanin, Bm" uniqKey="Matanin B">BM Matanin</name>
</author>
<author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name>
</author>
<author><name sortKey="Meng, Xj" uniqKey="Meng X">XJ Meng</name>
</author>
<author><name sortKey="Zhang, C" uniqKey="Zhang C">C Zhang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meng, Xj" uniqKey="Meng X">XJ Meng</name>
</author>
<author><name sortKey="Paul, Ps" uniqKey="Paul P">PS Paul</name>
</author>
<author><name sortKey="Halbur, Pg" uniqKey="Halbur P">PG Halbur</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meuleman, P" uniqKey="Meuleman P">P Meuleman</name>
</author>
<author><name sortKey="Albecka, A" uniqKey="Albecka A">A Albecka</name>
</author>
<author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S Belouzard</name>
</author>
<author><name sortKey="Vercauteren, K" uniqKey="Vercauteren K">K Vercauteren</name>
</author>
<author><name sortKey="Verhoye, L" uniqKey="Verhoye L">L Verhoye</name>
</author>
<author><name sortKey="Wychowski, C" uniqKey="Wychowski C">C Wychowski</name>
</author>
<author><name sortKey="Lerouxroels, G" uniqKey="Lerouxroels G">G Lerouxroels</name>
</author>
<author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name>
</author>
<author><name sortKey="Dubuisson, J" uniqKey="Dubuisson J">J Dubuisson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Michon, C" uniqKey="Michon C">C Michon</name>
</author>
<author><name sortKey="Langella, P" uniqKey="Langella P">P Langella</name>
</author>
<author><name sortKey="Eijsink, Vgh" uniqKey="Eijsink V">VGH Eijsink</name>
</author>
<author><name sortKey="Mathiesen, G" uniqKey="Mathiesen G">G Mathiesen</name>
</author>
<author><name sortKey="Chatel, Jm" uniqKey="Chatel J">JM Chatel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Millet, Jk" uniqKey="Millet J">JK Millet</name>
</author>
<author><name sortKey="Seron, K" uniqKey="Seron K">K Séron</name>
</author>
<author><name sortKey="Labitt, Rn" uniqKey="Labitt R">RN Labitt</name>
</author>
<author><name sortKey="Danneels, A" uniqKey="Danneels A">A Danneels</name>
</author>
<author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name>
</author>
<author><name sortKey="Whittaker, Gr" uniqKey="Whittaker G">GR Whittaker</name>
</author>
<author><name sortKey="Dubuisson, J" uniqKey="Dubuisson J">J Dubuisson</name>
</author>
<author><name sortKey="Belouzard, S" uniqKey="Belouzard S">S Belouzard</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Moncla, Bj" uniqKey="Moncla B">BJ Moncla</name>
</author>
<author><name sortKey="Pryke, K" uniqKey="Pryke K">K Pryke</name>
</author>
<author><name sortKey="Rohan, Lc" uniqKey="Rohan L">LC Rohan</name>
</author>
<author><name sortKey="Graebing, Pw" uniqKey="Graebing P">PW Graebing</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mori, T" uniqKey="Mori T">T Mori</name>
</author>
<author><name sortKey="O Eefe, Br" uniqKey="O Eefe B">BR O’Keefe</name>
</author>
<author><name sortKey="Sowder, Rc" uniqKey="Sowder R">RC Sowder</name>
</author>
<author><name sortKey="Bringans, S" uniqKey="Bringans S">S Bringans</name>
</author>
<author><name sortKey="Gardella, R" uniqKey="Gardella R">R Gardella</name>
</author>
<author><name sortKey="Berg, S" uniqKey="Berg S">S Berg</name>
</author>
<author><name sortKey="Cochran, P" uniqKey="Cochran P">P Cochran</name>
</author>
<author><name sortKey="Turpin, Ja" uniqKey="Turpin J">JA Turpin</name>
</author>
<author><name sortKey="Buckheit, Rw" uniqKey="Buckheit R">RW Buckheit</name>
</author>
<author><name sortKey="Mcmahon, Jb" uniqKey="Mcmahon J">JB Mcmahon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Moulaei, T" uniqKey="Moulaei T">T Moulaei</name>
</author>
<author><name sortKey="Alexandre, Kb" uniqKey="Alexandre K">KB Alexandre</name>
</author>
<author><name sortKey="Shenoy, Sr" uniqKey="Shenoy S">SR Shenoy</name>
</author>
<author><name sortKey="Meyerson, Jr" uniqKey="Meyerson J">JR Meyerson</name>
</author>
<author><name sortKey="Krumpe, Lr" uniqKey="Krumpe L">LR Krumpe</name>
</author>
<author><name sortKey="Constantine, B" uniqKey="Constantine B">B Constantine</name>
</author>
<author><name sortKey="Wilson, J" uniqKey="Wilson J">J Wilson</name>
</author>
<author><name sortKey="Buckheit, Rw" uniqKey="Buckheit R">RW Buckheit</name>
</author>
<author><name sortKey="Mcmahon, Jb" uniqKey="Mcmahon J">JB McMahon</name>
</author>
<author><name sortKey="Subramaniam, S" uniqKey="Subramaniam S">S Subramaniam</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nixon, B" uniqKey="Nixon B">B Nixon</name>
</author>
<author><name sortKey="Stefanidou, M" uniqKey="Stefanidou M">M Stefanidou</name>
</author>
<author><name sortKey="Mesquita, Pm" uniqKey="Mesquita P">PM Mesquita</name>
</author>
<author><name sortKey="Fakioglu, E" uniqKey="Fakioglu E">E Fakioglu</name>
</author>
<author><name sortKey="Segarra, T" uniqKey="Segarra T">T Segarra</name>
</author>
<author><name sortKey="Rohan, L" uniqKey="Rohan L">L Rohan</name>
</author>
<author><name sortKey="Halford, W" uniqKey="Halford W">W Halford</name>
</author>
<author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name>
</author>
<author><name sortKey="Herold, Bc" uniqKey="Herold B">BC Herold</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="O Eefe, Br" uniqKey="O Eefe B">BR O’Keefe</name>
</author>
<author><name sortKey="Giomarelli, B" uniqKey="Giomarelli B">B Giomarelli</name>
</author>
<author><name sortKey="Barnard, Dl" uniqKey="Barnard D">DL Barnard</name>
</author>
<author><name sortKey="Shenoy, Sr" uniqKey="Shenoy S">SR Shenoy</name>
</author>
<author><name sortKey="Chan, Pks" uniqKey="Chan P">PKS Chan</name>
</author>
<author><name sortKey="Mcmahon, Jb" uniqKey="Mcmahon J">JB Mcmahon</name>
</author>
<author><name sortKey="Palmer, Ke" uniqKey="Palmer K">KE Palmer</name>
</author>
<author><name sortKey="Barnett, Bw" uniqKey="Barnett B">BW Barnett</name>
</author>
<author><name sortKey="Meyerholz, Dk" uniqKey="Meyerholz D">DK Meyerholz</name>
</author>
<author><name sortKey="Wohlfordlenane, Cl" uniqKey="Wohlfordlenane C">CL Wohlfordlenane</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Urtasun, N" uniqKey="Urtasun N">N Urtasun</name>
</author>
<author><name sortKey="Baieli, Mf" uniqKey="Baieli M">MF Baieli</name>
</author>
<author><name sortKey="Cascone, O" uniqKey="Cascone O">O Cascone</name>
</author>
<author><name sortKey="Wolman, Fj" uniqKey="Wolman F">FJ Wolman</name>
</author>
<author><name sortKey="Miranda, Mv" uniqKey="Miranda M">MV Miranda</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vamvaka, E" uniqKey="Vamvaka E">E Vamvaka</name>
</author>
<author><name sortKey="Arcalis, E" uniqKey="Arcalis E">E Arcalis</name>
</author>
<author><name sortKey="Ramessar, K" uniqKey="Ramessar K">K Ramessar</name>
</author>
<author><name sortKey="Evans, A" uniqKey="Evans A">A Evans</name>
</author>
<author><name sortKey="O Eefe, Br" uniqKey="O Eefe B">BR O’Keefe</name>
</author>
<author><name sortKey="Shattock, Rj" uniqKey="Shattock R">RJ Shattock</name>
</author>
<author><name sortKey="Medina, V" uniqKey="Medina V">V Medina</name>
</author>
<author><name sortKey="Stoger, E" uniqKey="Stoger E">E Stöger</name>
</author>
<author><name sortKey="Christou, P" uniqKey="Christou P">P Christou</name>
</author>
<author><name sortKey="Capell, T" uniqKey="Capell T">T Capell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vincenzi, S" uniqKey="Vincenzi S">S Vincenzi</name>
</author>
<author><name sortKey="Zoccatelli, G" uniqKey="Zoccatelli G">G Zoccatelli</name>
</author>
<author><name sortKey="Perbellini, F" uniqKey="Perbellini F">F Perbellini</name>
</author>
<author><name sortKey="Rizzi, C" uniqKey="Rizzi C">C Rizzi</name>
</author>
<author><name sortKey="Chignola, R" uniqKey="Chignola R">R Chignola</name>
</author>
<author><name sortKey="Curioni, A" uniqKey="Curioni A">A Curioni</name>
</author>
<author><name sortKey="Peruffo, Ad" uniqKey="Peruffo A">AD Peruffo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wu, Wh" uniqKey="Wu W">WH Wu</name>
</author>
<author><name sortKey="Fang, Y" uniqKey="Fang Y">Y Fang</name>
</author>
<author><name sortKey="Rowland, Rr" uniqKey="Rowland R">RR Rowland</name>
</author>
<author><name sortKey="Lawson, Sr" uniqKey="Lawson S">SR Lawson</name>
</author>
<author><name sortKey="Christopher Hennings, J" uniqKey="Christopher Hennings J">J Christopher-Hennings</name>
</author>
<author><name sortKey="Yoon, Kj" uniqKey="Yoon K">KJ Yoon</name>
</author>
<author><name sortKey="Nelson, Ea" uniqKey="Nelson E">EA Nelson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Xue, J" uniqKey="Xue J">J Xue</name>
</author>
<author><name sortKey="Gao, Y" uniqKey="Gao Y">Y Gao</name>
</author>
<author><name sortKey="Hoorelbeke, B" uniqKey="Hoorelbeke B">B Hoorelbeke</name>
</author>
<author><name sortKey="Kagiampakis, I" uniqKey="Kagiampakis I">I Kagiampakis</name>
</author>
<author><name sortKey="Zhao, B" uniqKey="Zhao B">B Zhao</name>
</author>
<author><name sortKey="Demeler, B" uniqKey="Demeler B">B Demeler</name>
</author>
<author><name sortKey="Balzarini, J" uniqKey="Balzarini J">J Balzarini</name>
</author>
<author><name sortKey="Liwang, Pj" uniqKey="Liwang P">PJ Liwang</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Ind Microbiol Biotechnol</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Ind. Microbiol. Biotechnol</journal-id>
<journal-title-group><journal-title>Journal of Industrial Microbiology & Biotechnology</journal-title>
</journal-title-group>
<issn pub-type="ppub">1367-5435</issn>
<issn pub-type="epub">1476-5535</issn>
<publisher><publisher-name>Springer International Publishing</publisher-name>
<publisher-loc>Cham</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">30046953</article-id>
<article-id pub-id-type="pmc">7088258</article-id>
<article-id pub-id-type="publisher-id">2061</article-id>
<article-id pub-id-type="doi">10.1007/s10295-018-2061-1</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Biotechnology Methods - Original Paper</subject>
</subj-group>
</article-categories>
<title-group><article-title>Surface-displayed porcine reproductive and respiratory syndrome virus from cell culture onto gram-positive enhancer matrix particles</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Lan</given-names>
</name>
<address><email>lanli@foxmail.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Qiao</surname>
<given-names>Xuwen</given-names>
</name>
<address><email>qxwshyi2011@hotmail.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Chen</surname>
<given-names>Jin</given-names>
</name>
<address><email>chenjin_abc@163.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhang</surname>
<given-names>Yuanpeng</given-names>
</name>
<address><email>yuanpengzhangnj@163.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Zheng</surname>
<given-names>Qisheng</given-names>
</name>
<address><phone>+86-25-84392008</phone>
<email>njcvc1302@163.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Hou</surname>
<given-names>Jibo</given-names>
</name>
<address><phone>+86-25-84392008</phone>
<email>houjiboccvv@163.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<aff id="Aff1"><label>1</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id>
<institution-id institution-id-type="GRID">grid.454840.9</institution-id>
<institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution>
<institution>Jiangsu Academy of Agricultural Sciences,</institution>
</institution-wrap>
Nanjing, 210014 Jiangsu China</aff>
<aff id="Aff2"><label>2</label>
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009 Jiangsu China</aff>
</contrib-group>
<pub-date pub-type="epub"><day>25</day>
<month>7</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="ppub"><year>2018</year>
</pub-date>
<volume>45</volume>
<issue>10</issue>
<fpage>889</fpage>
<lpage>898</lpage>
<history><date date-type="received"><day>25</day>
<month>1</month>
<year>2018</year>
</date>
<date date-type="accepted"><day>13</day>
<month>7</month>
<year>2018</year>
</date>
</history>
<permissions><copyright-statement>© Society for Industrial Microbiology and Biotechnology 2018</copyright-statement>
<license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p>
</license>
</permissions>
<abstract id="Abs1"><p id="Par1">Vaccine immunization is now one of the most effective ways to control porcine reproductive and respiratory syndrome virus (PRRSV) infection. Impurity is one of the main factors affecting vaccine safety and efficacy. Here we present a novel innovative PRRSV purification approach based on surface display technology. First, a bifunctional protein PA-GRFT (protein anchor-griffithsin), the crucial factor in the purification process, was successfully produced in <italic>Escherichia coli</italic>
yielding 80 mg/L of broth culture. Then PRRSV purification was performed by incubation of PA-GRFT with PRRSV and gram-positive enhancer matrix (GEM) particles, followed by centrifugation to collect virions loaded onto GEM particles. Our results showed that most of the bulk impurities had been removed, and PA-GRFT could capture PRRSV onto GEM particles. Our lactic acid bacteria-based purification method, which is promising as ease of operation, low cost and easy to scale-up, may represent a candidate method for the large-scale purification of this virus for vaccine production.</p>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>Lactic acid bacteria</kwd>
<kwd>GEM particles</kwd>
<kwd>Purification</kwd>
<kwd>PRRSV</kwd>
<kwd>Griffithsin</kwd>
</kwd-group>
<funding-group><award-group><funding-source><institution>the Special Fund for Agro-scientific Research in the Public Interest</institution>
</funding-source>
<award-id>201303046</award-id>
<principal-award-recipient><name><surname>Hou</surname>
<given-names>Jibo</given-names>
</name>
</principal-award-recipient>
</award-group>
</funding-group>
<funding-group><award-group><funding-source><institution>the Independent Innovation of Agricultural Sciences Program of Jiangsu Province</institution>
</funding-source>
<award-id>cx (14)2089</award-id>
<principal-award-recipient><name><surname>Zheng</surname>
<given-names>Qisheng</given-names>
</name>
</principal-award-recipient>
</award-group>
</funding-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© Society for Industrial Microbiology and Biotechnology 2018</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000275 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000275 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV2 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:7088258 |texte= Surface-displayed porcine reproductive and respiratory syndrome virus from cell culture onto gram-positive enhancer matrix particles }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:30046953" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a CovidV2
![]() | This area was generated with Dilib version V0.6.33. | ![]() |