Transcriptome sequencing and development of an expression microarray platform for the domestic ferret
Identifieur interne : 000194 ( Pmc/Curation ); précédent : 000193; suivant : 000195Transcriptome sequencing and development of an expression microarray platform for the domestic ferret
Auteurs : Carl E. Bruder [États-Unis] ; Suxia Yao [États-Unis] ; Francis Larson [États-Unis] ; Jeremy V. Camp [États-Unis] ; Ronald Tapp [États-Unis] ; Alexis Mcbrayer [États-Unis] ; Nicholas Powers [États-Unis] ; Willy Valdivia Granda [États-Unis] ; Colleen B. Jonsson [États-Unis]Source :
- BMC Genomics [ 1471-2164 ] ; 2010.
Abstract
The ferret (
We produced more than 500000 sequence reads that were assembled into 16000 partial ferret genes. These genes were combined with the available ferret sequences in the GenBank to develop a ferret specific microarray platform. Using this array, we detected tissue specific expression patterns which were confirmed by quantitative real time PCR assays. We also present a set of 41 ferret genes with even transcription profiles across the tested tissues, indicating their usefulness as housekeeping genes.
The tools developed in this study allow for functional genomic analysis and make further development of reagents for the ferret model possible.
The online version of this article (doi:10.1186/1471-2164-11-251) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1471-2164-11-251
PubMed: 20403183
PubMed Central: 2873475
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000194
Links to Exploration step
PMC:2873475Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transcriptome sequencing and development of an expression microarray platform for the domestic ferret</title><author><name sortKey="Bruder, Carl E" sort="Bruder, Carl E" uniqKey="Bruder C" first="Carl E" last="Bruder">Carl E. Bruder</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Yao, Suxia" sort="Yao, Suxia" uniqKey="Yao S" first="Suxia" last="Yao">Suxia Yao</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Larson, Francis" sort="Larson, Francis" uniqKey="Larson F" first="Francis" last="Larson">Francis Larson</name><affiliation wicri:level="2"><nlm:aff id="Aff2">Orion Integrated Biosciences Inc, 265 Centre Ave, Suite 1R, New Rochelle, NY 10805 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Orion Integrated Biosciences Inc, 265 Centre Ave, Suite 1R, New Rochelle</wicri:cityArea></affiliation></author><author><name sortKey="Camp, Jeremy V" sort="Camp, Jeremy V" uniqKey="Camp J" first="Jeremy V" last="Camp">Jeremy V. Camp</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Tapp, Ronald" sort="Tapp, Ronald" uniqKey="Tapp R" first="Ronald" last="Tapp">Ronald Tapp</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Mcbrayer, Alexis" sort="Mcbrayer, Alexis" uniqKey="Mcbrayer A" first="Alexis" last="Mcbrayer">Alexis Mcbrayer</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Powers, Nicholas" sort="Powers, Nicholas" uniqKey="Powers N" first="Nicholas" last="Powers">Nicholas Powers</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Valdivia Granda, Willy" sort="Valdivia Granda, Willy" uniqKey="Valdivia Granda W" first="Willy" last="Valdivia Granda">Willy Valdivia Granda</name><affiliation wicri:level="2"><nlm:aff id="Aff2">Orion Integrated Biosciences Inc, 265 Centre Ave, Suite 1R, New Rochelle, NY 10805 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Orion Integrated Biosciences Inc, 265 Centre Ave, Suite 1R, New Rochelle</wicri:cityArea></affiliation></author><author><name sortKey="Jonsson, Colleen B" sort="Jonsson, Colleen B" uniqKey="Jonsson C" first="Colleen B" last="Jonsson">Colleen B. Jonsson</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation><affiliation><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.266623.5</institution-id><institution-id institution-id-type="ISNI">0000000121131622</institution-id><institution>Center for Predictive Medicine For Biodefense and Emerging Infectious Disease,</institution><institution>University of Louisville,</institution></institution-wrap>KY 40292 USA</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20403183</idno><idno type="pmc">2873475</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2873475</idno><idno type="RBID">PMC:2873475</idno><idno type="doi">10.1186/1471-2164-11-251</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000194</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000194</idno><idno type="wicri:Area/Pmc/Curation">000194</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000194</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Transcriptome sequencing and development of an expression microarray platform for the domestic ferret</title><author><name sortKey="Bruder, Carl E" sort="Bruder, Carl E" uniqKey="Bruder C" first="Carl E" last="Bruder">Carl E. Bruder</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Yao, Suxia" sort="Yao, Suxia" uniqKey="Yao S" first="Suxia" last="Yao">Suxia Yao</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Larson, Francis" sort="Larson, Francis" uniqKey="Larson F" first="Francis" last="Larson">Francis Larson</name><affiliation wicri:level="2"><nlm:aff id="Aff2">Orion Integrated Biosciences Inc, 265 Centre Ave, Suite 1R, New Rochelle, NY 10805 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Orion Integrated Biosciences Inc, 265 Centre Ave, Suite 1R, New Rochelle</wicri:cityArea></affiliation></author><author><name sortKey="Camp, Jeremy V" sort="Camp, Jeremy V" uniqKey="Camp J" first="Jeremy V" last="Camp">Jeremy V. Camp</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Tapp, Ronald" sort="Tapp, Ronald" uniqKey="Tapp R" first="Ronald" last="Tapp">Ronald Tapp</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Mcbrayer, Alexis" sort="Mcbrayer, Alexis" uniqKey="Mcbrayer A" first="Alexis" last="Mcbrayer">Alexis Mcbrayer</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Powers, Nicholas" sort="Powers, Nicholas" uniqKey="Powers N" first="Nicholas" last="Powers">Nicholas Powers</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation></author><author><name sortKey="Valdivia Granda, Willy" sort="Valdivia Granda, Willy" uniqKey="Valdivia Granda W" first="Willy" last="Valdivia Granda">Willy Valdivia Granda</name><affiliation wicri:level="2"><nlm:aff id="Aff2">Orion Integrated Biosciences Inc, 265 Centre Ave, Suite 1R, New Rochelle, NY 10805 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Orion Integrated Biosciences Inc, 265 Centre Ave, Suite 1R, New Rochelle</wicri:cityArea></affiliation></author><author><name sortKey="Jonsson, Colleen B" sort="Jonsson, Colleen B" uniqKey="Jonsson C" first="Colleen B" last="Jonsson">Colleen B. Jonsson</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Alabama</region></placeName><wicri:cityArea>2000 Ninth Ave South, Birmingham</wicri:cityArea></affiliation><affiliation><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.266623.5</institution-id><institution-id institution-id-type="ISNI">0000000121131622</institution-id><institution>Center for Predictive Medicine For Biodefense and Emerging Infectious Disease,</institution><institution>University of Louisville,</institution></institution-wrap>KY 40292 USA</nlm:aff></affiliation></author></analytic><series><title level="j">BMC Genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>The ferret (<italic>Mustela putorius furo</italic>) represents an attractive animal model for the study of respiratory diseases, including influenza. Despite its importance for biomedical research, the number of reagents for molecular and immunological analysis is restricted. We present here a parallel sequencing effort to produce an extensive EST (expressed sequence tags) dataset derived from a normalized ferret cDNA library made from mRNA from ferret blood, liver, lung, spleen and brain.</p></sec><sec><title>Results</title><p>We produced more than 500000 sequence reads that were assembled into 16000 partial ferret genes. These genes were combined with the available ferret sequences in the GenBank to develop a ferret specific microarray platform. Using this array, we detected tissue specific expression patterns which were confirmed by quantitative real time PCR assays. We also present a set of 41 ferret genes with even transcription profiles across the tested tissues, indicating their usefulness as housekeeping genes.</p></sec><sec><title>Conclusion</title><p>The tools developed in this study allow for functional genomic analysis and make further development of reagents for the ferret model possible.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1471-2164-11-251) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chu, Yk" uniqKey="Chu Y">YK Chu</name></author><author><name sortKey="Ali, Gd" uniqKey="Ali G">GD Ali</name></author><author><name sortKey="Jia, F" uniqKey="Jia F">F Jia</name></author><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Kelvin, D" uniqKey="Kelvin D">D Kelvin</name></author><author><name sortKey="Couch, Rc" uniqKey="Couch R">RC Couch</name></author><author><name sortKey="Harrod, Ks" uniqKey="Harrod K">KS Harrod</name></author><author><name sortKey="Hutt, Ja" uniqKey="Hutt J">JA Hutt</name></author><author><name sortKey="Cameron, C" uniqKey="Cameron C">C Cameron</name></author><author><name sortKey="Weiss, Sr" uniqKey="Weiss S">SR Weiss</name></author><author><name sortKey="Jonsson, Cb" uniqKey="Jonsson C">CB Jonsson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Larin, Nm" uniqKey="Larin N">NM Larin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lipatov, As" uniqKey="Lipatov A">AS Lipatov</name></author><author><name sortKey="Kwon, Yk" uniqKey="Kwon Y">YK Kwon</name></author><author><name sortKey="Pantin Jackwood, Mj" uniqKey="Pantin Jackwood M">MJ Pantin-Jackwood</name></author><author><name sortKey="Swayne, De" uniqKey="Swayne D">DE Swayne</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kirkeby, S" uniqKey="Kirkeby S">S Kirkeby</name></author><author><name sortKey="Martel, Cj" uniqKey="Martel C">CJ Martel</name></author><author><name sortKey="Aasted, B" uniqKey="Aasted B">B Aasted</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maher, Ja" uniqKey="Maher J">JA Maher</name></author><author><name sortKey="Destefano, J" uniqKey="Destefano J">J DeStefano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matsuoka, Y" uniqKey="Matsuoka Y">Y Matsuoka</name></author><author><name sortKey="Lamirande, Ew" uniqKey="Lamirande E">EW Lamirande</name></author><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K Subbarao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reuman, Pd" uniqKey="Reuman P">PD Reuman</name></author><author><name sortKey="Keely, S" uniqKey="Keely S">S Keely</name></author><author><name sortKey="Schiff, Gm" uniqKey="Schiff G">GM Schiff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yen, Hl" uniqKey="Yen H">HL Yen</name></author><author><name sortKey="Lipatov, As" uniqKey="Lipatov A">AS Lipatov</name></author><author><name sortKey="Ilyushina, Na" uniqKey="Ilyushina N">NA Ilyushina</name></author><author><name sortKey="Govorkova, Ea" uniqKey="Govorkova E">EA Govorkova</name></author><author><name sortKey="Franks, J" uniqKey="Franks J">J Franks</name></author><author><name sortKey="Yilmaz, N" uniqKey="Yilmaz N">N Yilmaz</name></author><author><name sortKey="Douglas, A" uniqKey="Douglas A">A Douglas</name></author><author><name sortKey="Hay, A" uniqKey="Hay A">A Hay</name></author><author><name sortKey="Krauss, S" uniqKey="Krauss S">S Krauss</name></author><author><name sortKey="Rehg, Je" uniqKey="Rehg J">JE Rehg</name></author><author><name sortKey="Hoffmann, E" uniqKey="Hoffmann E">E Hoffmann</name></author><author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laan, Jw Van Der" uniqKey="Laan J">JW van der Laan</name></author><author><name sortKey="Herberts, C" uniqKey="Herberts C">C Herberts</name></author><author><name sortKey="Lambkin Williams, R" uniqKey="Lambkin Williams R">R Lambkin-Williams</name></author><author><name sortKey="Boyers, A" uniqKey="Boyers A">A Boyers</name></author><author><name sortKey="Mann, Aj" uniqKey="Mann A">AJ Mann</name></author><author><name sortKey="Oxford, J" uniqKey="Oxford J">J Oxford</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Green, Re" uniqKey="Green R">RE Green</name></author><author><name sortKey="Krause, J" uniqKey="Krause J">J Krause</name></author><author><name sortKey="Ptak, Se" uniqKey="Ptak S">SE Ptak</name></author><author><name sortKey="Briggs, Aw" uniqKey="Briggs A">AW Briggs</name></author><author><name sortKey="Ronan, Mt" uniqKey="Ronan M">MT Ronan</name></author><author><name sortKey="Simons, Jf" uniqKey="Simons J">JF Simons</name></author><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="Egholm, M" uniqKey="Egholm M">M Egholm</name></author><author><name sortKey="Rothberg, Jm" uniqKey="Rothberg J">JM Rothberg</name></author><author><name sortKey="Paunovic, M" uniqKey="Paunovic M">M Paunovic</name></author><author><name sortKey="Paabo, S" uniqKey="Paabo S">S Paabo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Margulies, M" uniqKey="Margulies M">M Margulies</name></author><author><name sortKey="Egholm, M" uniqKey="Egholm M">M Egholm</name></author><author><name sortKey="Altman, We" uniqKey="Altman W">WE Altman</name></author><author><name sortKey="Attiya, S" uniqKey="Attiya S">S Attiya</name></author><author><name sortKey="Bader, Js" uniqKey="Bader J">JS Bader</name></author><author><name sortKey="Bemben, La" uniqKey="Bemben L">LA Bemben</name></author><author><name sortKey="Berka, J" uniqKey="Berka J">J Berka</name></author><author><name sortKey="Braverman, Ms" uniqKey="Braverman M">MS Braverman</name></author><author><name sortKey="Chen, Yj" uniqKey="Chen Y">YJ Chen</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z Chen</name></author><author><name sortKey="Dewell, Sb" uniqKey="Dewell S">SB Dewell</name></author><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="Fierro, Jm" uniqKey="Fierro J">JM Fierro</name></author><author><name sortKey="Gomes, Xv" uniqKey="Gomes X">XV Gomes</name></author><author><name sortKey="Godwin, Bc" uniqKey="Godwin B">BC Godwin</name></author><author><name sortKey="He, W" uniqKey="He W">W He</name></author><author><name sortKey="Helgesen, S" uniqKey="Helgesen S">S Helgesen</name></author><author><name sortKey="Ho, Ch" uniqKey="Ho C">CH Ho</name></author><author><name sortKey="Irzyk, Gp" uniqKey="Irzyk G">GP Irzyk</name></author><author><name sortKey="Jando, Sc" uniqKey="Jando S">SC Jando</name></author><author><name sortKey="Alenquer, Ml" uniqKey="Alenquer M">ML Alenquer</name></author><author><name sortKey="Jarvie, Tp" uniqKey="Jarvie T">TP Jarvie</name></author><author><name sortKey="Jirage, Kb" uniqKey="Jirage K">KB Jirage</name></author><author><name sortKey="Kim, Jb" uniqKey="Kim J">JB Kim</name></author><author><name sortKey="Knight, Jr" uniqKey="Knight J">JR Knight</name></author><author><name sortKey="Lanza, Jr" uniqKey="Lanza J">JR Lanza</name></author><author><name sortKey="Leamon, Jh" uniqKey="Leamon J">JH Leamon</name></author><author><name sortKey="Lefkowitz, Sm" uniqKey="Lefkowitz S">SM Lefkowitz</name></author><author><name sortKey="Lei, M" uniqKey="Lei M">M Lei</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Lohman, Kl" uniqKey="Lohman K">KL Lohman</name></author><author><name sortKey="Lu, H" uniqKey="Lu H">H Lu</name></author><author><name sortKey="Makhijani, Vb" uniqKey="Makhijani V">VB Makhijani</name></author><author><name sortKey="Mcdade, Ke" uniqKey="Mcdade K">KE McDade</name></author><author><name sortKey="Mckenna, Mp" uniqKey="Mckenna M">MP McKenna</name></author><author><name sortKey="Myers, Ew" uniqKey="Myers E">EW Myers</name></author><author><name sortKey="Nickerson, E" uniqKey="Nickerson E">E Nickerson</name></author><author><name sortKey="Nobile, Jr" uniqKey="Nobile J">JR Nobile</name></author><author><name sortKey="Plant, R" uniqKey="Plant R">R Plant</name></author><author><name sortKey="Puc, Bp" uniqKey="Puc B">BP Puc</name></author><author><name sortKey="Ronan, Mt" uniqKey="Ronan M">MT Ronan</name></author><author><name sortKey="Roth, Gt" uniqKey="Roth G">GT Roth</name></author><author><name sortKey="Sarkis, Gj" uniqKey="Sarkis G">GJ Sarkis</name></author><author><name sortKey="Simons, Jf" uniqKey="Simons J">JF Simons</name></author><author><name sortKey="Simpson, Jw" uniqKey="Simpson J">JW Simpson</name></author><author><name sortKey="Srinivasan, M" uniqKey="Srinivasan M">M Srinivasan</name></author><author><name sortKey="Tartaro, Kr" uniqKey="Tartaro K">KR Tartaro</name></author><author><name sortKey="Tomasz, A" uniqKey="Tomasz A">A Tomasz</name></author><author><name sortKey="Vogt, Ka" uniqKey="Vogt K">KA Vogt</name></author><author><name sortKey="Volkmer, Ga" uniqKey="Volkmer G">GA Volkmer</name></author><author><name sortKey="Wang, Sh" uniqKey="Wang S">SH Wang</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Weiner, Mp" uniqKey="Weiner M">MP Weiner</name></author><author><name sortKey="Yu, P" uniqKey="Yu P">P Yu</name></author><author><name sortKey="Begley, Rf" uniqKey="Begley R">RF Begley</name></author><author><name sortKey="Rothberg, Jm" uniqKey="Rothberg J">JM Rothberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wheeler, Da" uniqKey="Wheeler D">DA Wheeler</name></author><author><name sortKey="Srinivasan, M" uniqKey="Srinivasan M">M Srinivasan</name></author><author><name sortKey="Egholm, M" uniqKey="Egholm M">M Egholm</name></author><author><name sortKey="Shen, Y" uniqKey="Shen Y">Y Shen</name></author><author><name sortKey="Chen, L" uniqKey="Chen L">L Chen</name></author><author><name sortKey="Mcguire, A" uniqKey="Mcguire A">A McGuire</name></author><author><name sortKey="He, W" uniqKey="He W">W He</name></author><author><name sortKey="Chen, Yj" uniqKey="Chen Y">YJ Chen</name></author><author><name sortKey="Makhijani, V" uniqKey="Makhijani V">V Makhijani</name></author><author><name sortKey="Roth, Gt" uniqKey="Roth G">GT Roth</name></author><author><name sortKey="Gomes, X" uniqKey="Gomes X">X Gomes</name></author><author><name sortKey="Tartaro, K" uniqKey="Tartaro K">K Tartaro</name></author><author><name sortKey="Niazi, F" uniqKey="Niazi F">F Niazi</name></author><author><name sortKey="Turcotte, Cl" uniqKey="Turcotte C">CL Turcotte</name></author><author><name sortKey="Irzyk, Gp" uniqKey="Irzyk G">GP Irzyk</name></author><author><name sortKey="Lupski, Jr" uniqKey="Lupski J">JR Lupski</name></author><author><name sortKey="Chinault, C" uniqKey="Chinault C">C Chinault</name></author><author><name sortKey="Song, Xz" uniqKey="Song X">XZ Song</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author><author><name sortKey="Yuan, Y" uniqKey="Yuan Y">Y Yuan</name></author><author><name sortKey="Nazareth, L" uniqKey="Nazareth L">L Nazareth</name></author><author><name sortKey="Qin, X" uniqKey="Qin X">X Qin</name></author><author><name sortKey="Muzny, Dm" uniqKey="Muzny D">DM Muzny</name></author><author><name sortKey="Margulies, M" uniqKey="Margulies M">M Margulies</name></author><author><name sortKey="Weinstock, Gm" uniqKey="Weinstock G">GM Weinstock</name></author><author><name sortKey="Gibbs, Ra" uniqKey="Gibbs R">RA Gibbs</name></author><author><name sortKey="Rothberg, Jm" uniqKey="Rothberg J">JM Rothberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Emrich, Sj" uniqKey="Emrich S">SJ Emrich</name></author><author><name sortKey="Barbazuk, Wb" uniqKey="Barbazuk W">WB Barbazuk</name></author><author><name sortKey="Li, L" uniqKey="Li L">L Li</name></author><author><name sortKey="Schnable, Ps" uniqKey="Schnable P">PS Schnable</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyer, E" uniqKey="Meyer E">E Meyer</name></author><author><name sortKey="Aglyamova, Gv" uniqKey="Aglyamova G">GV Aglyamova</name></author><author><name sortKey="Wang, S" uniqKey="Wang S">S Wang</name></author><author><name sortKey="Buchanan Carter, J" uniqKey="Buchanan Carter J">J Buchanan-Carter</name></author><author><name sortKey="Abrego, D" uniqKey="Abrego D">D Abrego</name></author><author><name sortKey="Colbourne, Jk" uniqKey="Colbourne J">JK Colbourne</name></author><author><name sortKey="Willis, Bl" uniqKey="Willis B">BL Willis</name></author><author><name sortKey="Matz, Mv" uniqKey="Matz M">MV Matz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Monaghan, Jr" uniqKey="Monaghan J">JR Monaghan</name></author><author><name sortKey="Epp, Lg" uniqKey="Epp L">LG Epp</name></author><author><name sortKey="Putta, S" uniqKey="Putta S">S Putta</name></author><author><name sortKey="Page, Rb" uniqKey="Page R">RB Page</name></author><author><name sortKey="Walker, Ja" uniqKey="Walker J">JA Walker</name></author><author><name sortKey="Beachy, Ck" uniqKey="Beachy C">CK Beachy</name></author><author><name sortKey="Zhu, W" uniqKey="Zhu W">W Zhu</name></author><author><name sortKey="Pao, Gm" uniqKey="Pao G">GM Pao</name></author><author><name sortKey="Verma, Im" uniqKey="Verma I">IM Verma</name></author><author><name sortKey="Hunter, T" uniqKey="Hunter T">T Hunter</name></author><author><name sortKey="Bryant, Sv" uniqKey="Bryant S">SV Bryant</name></author><author><name sortKey="Gardiner, Dm" uniqKey="Gardiner D">DM Gardiner</name></author><author><name sortKey="Harkins, Tt" uniqKey="Harkins T">TT Harkins</name></author><author><name sortKey="Voss, Sr" uniqKey="Voss S">SR Voss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vera, Jc" uniqKey="Vera J">JC Vera</name></author><author><name sortKey="Wheat, Cw" uniqKey="Wheat C">CW Wheat</name></author><author><name sortKey="Fescemyer, Hw" uniqKey="Fescemyer H">HW Fescemyer</name></author><author><name sortKey="Frilander, Mj" uniqKey="Frilander M">MJ Frilander</name></author><author><name sortKey="Crawford, Dl" uniqKey="Crawford D">DL Crawford</name></author><author><name sortKey="Hanski, I" uniqKey="Hanski I">I Hanski</name></author><author><name sortKey="Marden, Jh" uniqKey="Marden J">JH Marden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fredericksen, Bl" uniqKey="Fredericksen B">BL Fredericksen</name></author><author><name sortKey="Keller, Bc" uniqKey="Keller B">BC Keller</name></author><author><name sortKey="Fornek, J" uniqKey="Fornek J">J Fornek</name></author><author><name sortKey="Katze, Mg" uniqKey="Katze M">MG Katze</name></author><author><name sortKey="Gale, M" uniqKey="Gale M">M Gale</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cameron, Mj" uniqKey="Cameron M">MJ Cameron</name></author><author><name sortKey="Ran, L" uniqKey="Ran L">L Ran</name></author><author><name sortKey="Xu, L" uniqKey="Xu L">L Xu</name></author><author><name sortKey="Danesh, A" uniqKey="Danesh A">A Danesh</name></author><author><name sortKey="Bermejo Martin, Jf" uniqKey="Bermejo Martin J">JF Bermejo-Martin</name></author><author><name sortKey="Cameron, Cm" uniqKey="Cameron C">CM Cameron</name></author><author><name sortKey="Muller, Mp" uniqKey="Muller M">MP Muller</name></author><author><name sortKey="Gold, Wl" uniqKey="Gold W">WL Gold</name></author><author><name sortKey="Richardson, Se" uniqKey="Richardson S">SE Richardson</name></author><author><name sortKey="Poutanen, Sm" uniqKey="Poutanen S">SM Poutanen</name></author><author><name sortKey="Willey, Bm" uniqKey="Willey B">BM Willey</name></author><author><name sortKey="Devries, Me" uniqKey="Devries M">ME DeVries</name></author><author><name sortKey="Fang, Y" uniqKey="Fang Y">Y Fang</name></author><author><name sortKey="Seneviratne, C" uniqKey="Seneviratne C">C Seneviratne</name></author><author><name sortKey="Bosinger, Se" uniqKey="Bosinger S">SE Bosinger</name></author><author><name sortKey="Persad, D" uniqKey="Persad D">D Persad</name></author><author><name sortKey="Wilkinson, P" uniqKey="Wilkinson P">P Wilkinson</name></author><author><name sortKey="Greller, Ld" uniqKey="Greller L">LD Greller</name></author><author><name sortKey="Somogyi, R" uniqKey="Somogyi R">R Somogyi</name></author><author><name sortKey="Humar, A" uniqKey="Humar A">A Humar</name></author><author><name sortKey="Keshavjee, S" uniqKey="Keshavjee S">S Keshavjee</name></author><author><name sortKey="Louie, M" uniqKey="Louie M">M Louie</name></author><author><name sortKey="Loeb, Mb" uniqKey="Loeb M">MB Loeb</name></author><author><name sortKey="Brunton, J" uniqKey="Brunton J">J Brunton</name></author><author><name sortKey="Mcgeer, Aj" uniqKey="Mcgeer A">AJ McGeer</name></author><author><name sortKey="Kelvin, Dj" uniqKey="Kelvin D">DJ Kelvin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cameron, Cm" uniqKey="Cameron C">CM Cameron</name></author><author><name sortKey="Cameron, Mj" uniqKey="Cameron M">MJ Cameron</name></author><author><name sortKey="Bermejo Martin, Jf" uniqKey="Bermejo Martin J">JF Bermejo-Martin</name></author><author><name sortKey="Ran, L" uniqKey="Ran L">L Ran</name></author><author><name sortKey="Xu, L" uniqKey="Xu L">L Xu</name></author><author><name sortKey="Turner, Pv" uniqKey="Turner P">PV Turner</name></author><author><name sortKey="Ran, R" uniqKey="Ran R">R Ran</name></author><author><name sortKey="Danesh, A" uniqKey="Danesh A">A Danesh</name></author><author><name sortKey="Fang, Y" uniqKey="Fang Y">Y Fang</name></author><author><name sortKey="Chan, Pk" uniqKey="Chan P">PK Chan</name></author><author><name sortKey="Mytle, N" uniqKey="Mytle N">N Mytle</name></author><author><name sortKey="Sullivan, Tj" uniqKey="Sullivan T">TJ Sullivan</name></author><author><name sortKey="Collins, Tl" uniqKey="Collins T">TL Collins</name></author><author><name sortKey="Johnson, Mg" uniqKey="Johnson M">MG Johnson</name></author><author><name sortKey="Medina, Jc" uniqKey="Medina J">JC Medina</name></author><author><name sortKey="Rowe, T" uniqKey="Rowe T">T Rowe</name></author><author><name sortKey="Kelvin, Dj" uniqKey="Kelvin D">DJ Kelvin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ramilo, O" uniqKey="Ramilo O">O Ramilo</name></author><author><name sortKey="Allman, W" uniqKey="Allman W">W Allman</name></author><author><name sortKey="Chung, W" uniqKey="Chung W">W Chung</name></author><author><name sortKey="Mejias, A" uniqKey="Mejias A">A Mejias</name></author><author><name sortKey="Ardura, M" uniqKey="Ardura M">M Ardura</name></author><author><name sortKey="Glaser, C" uniqKey="Glaser C">C Glaser</name></author><author><name sortKey="Wittkowski, Km" uniqKey="Wittkowski K">KM Wittkowski</name></author><author><name sortKey="Piqueras, B" uniqKey="Piqueras B">B Piqueras</name></author><author><name sortKey="Banchereau, J" uniqKey="Banchereau J">J Banchereau</name></author><author><name sortKey="Palucka, Ak" uniqKey="Palucka A">AK Palucka</name></author><author><name sortKey="Chaussabel, D" uniqKey="Chaussabel D">D Chaussabel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kobasa, D" uniqKey="Kobasa D">D Kobasa</name></author><author><name sortKey="Jones, Sm" uniqKey="Jones S">SM Jones</name></author><author><name sortKey="Shinya, K" uniqKey="Shinya K">K Shinya</name></author><author><name sortKey="Kash, Jc" uniqKey="Kash J">JC Kash</name></author><author><name sortKey="Copps, J" uniqKey="Copps J">J Copps</name></author><author><name sortKey="Ebihara, H" uniqKey="Ebihara H">H Ebihara</name></author><author><name sortKey="Hatta, Y" uniqKey="Hatta Y">Y Hatta</name></author><author><name sortKey="Kim, Jh" uniqKey="Kim J">JH Kim</name></author><author><name sortKey="Halfmann, P" uniqKey="Halfmann P">P Halfmann</name></author><author><name sortKey="Hatta, M" uniqKey="Hatta M">M Hatta</name></author><author><name sortKey="Feldmann, F" uniqKey="Feldmann F">F Feldmann</name></author><author><name sortKey="Alimonti, Jb" uniqKey="Alimonti J">JB Alimonti</name></author><author><name sortKey="Fernando, L" uniqKey="Fernando L">L Fernando</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Katze, Mg" uniqKey="Katze M">MG Katze</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H Feldmann</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Querec, Td" uniqKey="Querec T">TD Querec</name></author><author><name sortKey="Akondy, Rs" uniqKey="Akondy R">RS Akondy</name></author><author><name sortKey="Lee, Ek" uniqKey="Lee E">EK Lee</name></author><author><name sortKey="Cao, W" uniqKey="Cao W">W Cao</name></author><author><name sortKey="Nakaya, Hi" uniqKey="Nakaya H">HI Nakaya</name></author><author><name sortKey="Teuwen, D" uniqKey="Teuwen D">D Teuwen</name></author><author><name sortKey="Pirani, A" uniqKey="Pirani A">A Pirani</name></author><author><name sortKey="Gernert, K" uniqKey="Gernert K">K Gernert</name></author><author><name sortKey="Deng, J" uniqKey="Deng J">J Deng</name></author><author><name sortKey="Marzolf, B" uniqKey="Marzolf B">B Marzolf</name></author><author><name sortKey="Kennedy, K" uniqKey="Kennedy K">K Kennedy</name></author><author><name sortKey="Wu, H" uniqKey="Wu H">H Wu</name></author><author><name sortKey="Bennouna, S" uniqKey="Bennouna S">S Bennouna</name></author><author><name sortKey="Oluoch, H" uniqKey="Oluoch H">H Oluoch</name></author><author><name sortKey="Miller, J" uniqKey="Miller J">J Miller</name></author><author><name sortKey="Vencio, Rz" uniqKey="Vencio R">RZ Vencio</name></author><author><name sortKey="Mulligan, M" uniqKey="Mulligan M">M Mulligan</name></author><author><name sortKey="Aderem, A" uniqKey="Aderem A">A Aderem</name></author><author><name sortKey="Ahmed, R" uniqKey="Ahmed R">R Ahmed</name></author><author><name sortKey="Pulendran, B" uniqKey="Pulendran B">B Pulendran</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bar Or, C" uniqKey="Bar Or C">C Bar-Or</name></author><author><name sortKey="Czosnek, H" uniqKey="Czosnek H">H Czosnek</name></author><author><name sortKey="Koltai, H" uniqKey="Koltai H">H Koltai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eisenberg, E" uniqKey="Eisenberg E">E Eisenberg</name></author><author><name sortKey="Levanon, Ey" uniqKey="Levanon E">EY Levanon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Govorkova, Ea" uniqKey="Govorkova E">EA Govorkova</name></author><author><name sortKey="Rehg, Je" uniqKey="Rehg J">JE Rehg</name></author><author><name sortKey="Krauss, S" uniqKey="Krauss S">S Krauss</name></author><author><name sortKey="Yen, Hl" uniqKey="Yen H">HL Yen</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Peiris, M" uniqKey="Peiris M">M Peiris</name></author><author><name sortKey="Nguyen, Td" uniqKey="Nguyen T">TD Nguyen</name></author><author><name sortKey="Hanh, Th" uniqKey="Hanh T">TH Hanh</name></author><author><name sortKey="Puthavathana, P" uniqKey="Puthavathana P">P Puthavathana</name></author><author><name sortKey="Long, Ht" uniqKey="Long H">HT Long</name></author><author><name sortKey="Buranathai, C" uniqKey="Buranathai C">C Buranathai</name></author><author><name sortKey="Lim, W" uniqKey="Lim W">W Lim</name></author><author><name sortKey="Webster, Rg" uniqKey="Webster R">RG Webster</name></author><author><name sortKey="Hoffmann, E" uniqKey="Hoffmann E">E Hoffmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Von Messling, V" uniqKey="Von Messling V">V von Messling</name></author><author><name sortKey="Springfeld, C" uniqKey="Springfeld C">C Springfeld</name></author><author><name sortKey="Devaux, P" uniqKey="Devaux P">P Devaux</name></author><author><name sortKey="Cattaneo, R" uniqKey="Cattaneo R">R Cattaneo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nemelka, Kw" uniqKey="Nemelka K">KW Nemelka</name></author><author><name sortKey="Brown, Aw" uniqKey="Brown A">AW Brown</name></author><author><name sortKey="Wallace, Sm" uniqKey="Wallace S">SM Wallace</name></author><author><name sortKey="Jones, E" uniqKey="Jones E">E Jones</name></author><author><name sortKey="Asher, Lv" uniqKey="Asher L">LV Asher</name></author><author><name sortKey="Pattarini, D" uniqKey="Pattarini D">D Pattarini</name></author><author><name sortKey="Applebee, L" uniqKey="Applebee L">L Applebee</name></author><author><name sortKey="Gilliland, Tc" uniqKey="Gilliland T">TC Gilliland</name></author><author><name sortKey="Guerry, P" uniqKey="Guerry P">P Guerry</name></author><author><name sortKey="Baqar, S" uniqKey="Baqar S">S Baqar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maelicke, A" uniqKey="Maelicke A">A Maelicke</name></author><author><name sortKey="Hoeffle Maas, A" uniqKey="Hoeffle Maas A">A Hoeffle-Maas</name></author><author><name sortKey="Ludwig, J" uniqKey="Ludwig J">J Ludwig</name></author><author><name sortKey="Maus, A" uniqKey="Maus A">A Maus</name></author><author><name sortKey="Samochocki, M" uniqKey="Samochocki M">M Samochocki</name></author><author><name sortKey="Jordis, U" uniqKey="Jordis U">U Jordis</name></author><author><name sortKey="Koepke, Ak" uniqKey="Koepke A">AK Koepke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Johansen, Sd" uniqKey="Johansen S">SD Johansen</name></author><author><name sortKey="Coucheron, Dh" uniqKey="Coucheron D">DH Coucheron</name></author><author><name sortKey="Andreassen, M" uniqKey="Andreassen M">M Andreassen</name></author><author><name sortKey="Karlsen, Bo" uniqKey="Karlsen B">BO Karlsen</name></author><author><name sortKey="Furmanek, T" uniqKey="Furmanek T">T Furmanek</name></author><author><name sortKey="Jorgensen, Te" uniqKey="Jorgensen T">TE Jorgensen</name></author><author><name sortKey="Emblem, A" uniqKey="Emblem A">A Emblem</name></author><author><name sortKey="Breines, R" uniqKey="Breines R">R Breines</name></author><author><name sortKey="Nordeide, Jt" uniqKey="Nordeide J">JT Nordeide</name></author><author><name sortKey="Moum, T" uniqKey="Moum T">T Moum</name></author><author><name sortKey="Nederbragt, Aj" uniqKey="Nederbragt A">AJ Nederbragt</name></author><author><name sortKey="Stenseth, Nc" uniqKey="Stenseth N">NC Stenseth</name></author><author><name sortKey="Jakobsen, Ks" uniqKey="Jakobsen K">KS Jakobsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Novaes, E" uniqKey="Novaes E">E Novaes</name></author><author><name sortKey="Drost, Dr" uniqKey="Drost D">DR Drost</name></author><author><name sortKey="Farmerie, Wg" uniqKey="Farmerie W">WG Farmerie</name></author><author><name sortKey="Pappas, Gj" uniqKey="Pappas G">GJ Pappas</name></author><author><name sortKey="Grattapaglia, D" uniqKey="Grattapaglia D">D Grattapaglia</name></author><author><name sortKey="Sederoff, Rr" uniqKey="Sederoff R">RR Sederoff</name></author><author><name sortKey="Kirst, M" uniqKey="Kirst M">M Kirst</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kawasaki, H" uniqKey="Kawasaki H">H Kawasaki</name></author><author><name sortKey="Crowley, Jc" uniqKey="Crowley J">JC Crowley</name></author><author><name sortKey="Livesey, Fj" uniqKey="Livesey F">FJ Livesey</name></author><author><name sortKey="Katz, Lc" uniqKey="Katz L">LC Katz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tricarico, C" uniqKey="Tricarico C">C Tricarico</name></author><author><name sortKey="Pinzani, P" uniqKey="Pinzani P">P Pinzani</name></author><author><name sortKey="Bianchi, S" uniqKey="Bianchi S">S Bianchi</name></author><author><name sortKey="Paglierani, M" uniqKey="Paglierani M">M Paglierani</name></author><author><name sortKey="Distante, V" uniqKey="Distante V">V Distante</name></author><author><name sortKey="Pazzagli, M" uniqKey="Pazzagli M">M Pazzagli</name></author><author><name sortKey="Bustin, Sa" uniqKey="Bustin S">SA Bustin</name></author><author><name sortKey="Orlando, C" uniqKey="Orlando C">C Orlando</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, X" uniqKey="Huang X">X Huang</name></author><author><name sortKey="Madan, A" uniqKey="Madan A">A Madan</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Genomics</journal-id><journal-id journal-id-type="iso-abbrev">BMC Genomics</journal-id><journal-title-group><journal-title>BMC Genomics</journal-title></journal-title-group><issn pub-type="epub">1471-2164</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">20403183</article-id><article-id pub-id-type="pmc">2873475</article-id><article-id pub-id-type="publisher-id">2845</article-id><article-id pub-id-type="doi">10.1186/1471-2164-11-251</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Transcriptome sequencing and development of an expression microarray platform for the domestic ferret</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Bruder</surname><given-names>Carl E</given-names></name><address><email>carl.bruder@gmail.com</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Yao</surname><given-names>Suxia</given-names></name><address><email>suxiayao@gmail.com</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Larson</surname><given-names>Francis</given-names></name><address><email>francis.larson@orionbiosciences.com</email></address><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Camp</surname><given-names>Jeremy V</given-names></name><address><email>campj@southernresearch.org</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Tapp</surname><given-names>Ronald</given-names></name><address><email>TAPP@southernresearch.org</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>McBrayer</surname><given-names>Alexis</given-names></name><address><email>mcbrayer@southernresearch.org</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Powers</surname><given-names>Nicholas</given-names></name><address><email>powersn@southernresearch.org</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Valdivia Granda</surname><given-names>Willy</given-names></name><address><email>willy.valdivia@orionbiosciences.com</email></address><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Jonsson</surname><given-names>Colleen B</given-names></name><address><email>cbjons01@louisville.edu</email></address><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff3">3</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="GRID">grid.454225.0</institution-id><institution-id institution-id-type="ISNI">0000000403768349</institution-id><institution>Southern Research Institute,</institution></institution-wrap>2000 Ninth Ave South, Birmingham, AL 35205 USA</aff><aff id="Aff2"><label>2</label>Orion Integrated Biosciences Inc, 265 Centre Ave, Suite 1R, New Rochelle, NY 10805 USA</aff><aff id="Aff3"><label>3</label><institution-wrap><institution-id institution-id-type="GRID">grid.266623.5</institution-id><institution-id institution-id-type="ISNI">0000000121131622</institution-id><institution>Center for Predictive Medicine For Biodefense and Emerging Infectious Disease,</institution><institution>University of Louisville,</institution></institution-wrap>KY 40292 USA</aff></contrib-group><pub-date pub-type="epub"><day>19</day><month>4</month><year>2010</year></pub-date><pub-date pub-type="pmc-release"><day>19</day><month>4</month><year>2010</year></pub-date><pub-date pub-type="collection"><year>2010</year></pub-date><volume>11</volume><elocation-id>251</elocation-id><history><date date-type="received"><day>17</day><month>12</month><year>2009</year></date><date date-type="accepted"><day>19</day><month>4</month><year>2010</year></date></history><permissions><copyright-statement>© Bruder et al; licensee BioMed Central Ltd. 2010</copyright-statement><license license-type="OpenAccess"><license-p>This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p>The ferret (<italic>Mustela putorius furo</italic>) represents an attractive animal model for the study of respiratory diseases, including influenza. Despite its importance for biomedical research, the number of reagents for molecular and immunological analysis is restricted. We present here a parallel sequencing effort to produce an extensive EST (expressed sequence tags) dataset derived from a normalized ferret cDNA library made from mRNA from ferret blood, liver, lung, spleen and brain.</p></sec><sec><title>Results</title><p>We produced more than 500000 sequence reads that were assembled into 16000 partial ferret genes. These genes were combined with the available ferret sequences in the GenBank to develop a ferret specific microarray platform. Using this array, we detected tissue specific expression patterns which were confirmed by quantitative real time PCR assays. We also present a set of 41 ferret genes with even transcription profiles across the tested tissues, indicating their usefulness as housekeeping genes.</p></sec><sec><title>Conclusion</title><p>The tools developed in this study allow for functional genomic analysis and make further development of reagents for the ferret model possible.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1471-2164-11-251) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Influenza</kwd><kwd>Influenza Virus</kwd><kwd>Severe Acute Respiratory Syndrome</kwd><kwd>Microarray Platform</kwd><kwd>Canine Distemper Virus</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© tbc 2010</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000194 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000194 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:2873475
|texte= Transcriptome sequencing and development of an expression microarray platform for the domestic ferret
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:20403183" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a CovidV2
| This area was generated with Dilib version V0.6.33. |  |