A highly conserved WDYPKCDRA epitope in the RNA directed RNA polymerase of human coronaviruses can be used as epitope-based universal vaccine design
Identifieur interne : 000191 ( Pmc/Curation ); précédent : 000190; suivant : 000192A highly conserved WDYPKCDRA epitope in the RNA directed RNA polymerase of human coronaviruses can be used as epitope-based universal vaccine design
Auteurs : Refat Sharmin ; Abul Bashar Mir Md Khademul IslamSource :
- BMC Bioinformatics [ 1471-2105 ] ; 2014.
Abstract
Coronaviruses are the diverse group of RNA virus. From 1960, six strains of human coronaviruses have emerged that includes SARS-CoV and the recent infection by deadly MERS-CoV which is now going to cause another outbreak. Prevention of these viruses is urgent and a universal vaccine for all strain could be a promising solution in this circumstance. In this study we aimed to design an epitope based vaccine against all strain of human coronavirus.
Multiple sequence alignment (MSA) approach was employed among spike (S), membrane (M), enveloped (E) and nucleocapsid (N) protein and replicase polyprotein 1ab to identify which one is highly conserve in all coronaviruses strains. Next, we use various
Here we suggest
The online version of this article (doi:10.1186/1471-2105-15-161) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1471-2105-15-161
PubMed: 24884408
PubMed Central: 4041900
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000191
Links to Exploration step
PMC:4041900Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A highly conserved WDYPKCDRA epitope in the RNA directed RNA polymerase of human coronaviruses can be used as epitope-based universal vaccine design</title><author><name sortKey="Sharmin, Refat" sort="Sharmin, Refat" uniqKey="Sharmin R" first="Refat" last="Sharmin">Refat Sharmin</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Islam, Abul Bashar Mir Md Khademul" sort="Islam, Abul Bashar Mir Md Khademul" uniqKey="Islam A" first="Abul Bashar Mir Md Khademul" last="Islam">Abul Bashar Mir Md Khademul Islam</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24884408</idno><idno type="pmc">4041900</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041900</idno><idno type="RBID">PMC:4041900</idno><idno type="doi">10.1186/1471-2105-15-161</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000191</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000191</idno><idno type="wicri:Area/Pmc/Curation">000191</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000191</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A highly conserved WDYPKCDRA epitope in the RNA directed RNA polymerase of human coronaviruses can be used as epitope-based universal vaccine design</title><author><name sortKey="Sharmin, Refat" sort="Sharmin, Refat" uniqKey="Sharmin R" first="Refat" last="Sharmin">Refat Sharmin</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Islam, Abul Bashar Mir Md Khademul" sort="Islam, Abul Bashar Mir Md Khademul" uniqKey="Islam A" first="Abul Bashar Mir Md Khademul" last="Islam">Abul Bashar Mir Md Khademul Islam</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">BMC Bioinformatics</title><idno type="eISSN">1471-2105</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Coronaviruses are the diverse group of RNA virus. From 1960, six strains of human coronaviruses have emerged that includes SARS-CoV and the recent infection by deadly MERS-CoV which is now going to cause another outbreak. Prevention of these viruses is urgent and a universal vaccine for all strain could be a promising solution in this circumstance. In this study we aimed to design an epitope based vaccine against all strain of human coronavirus.</p></sec><sec><title>Results</title><p>Multiple sequence alignment (MSA) approach was employed among spike (S), membrane (M), enveloped (E) and nucleocapsid (N) protein and replicase polyprotein 1ab to identify which one is highly conserve in all coronaviruses strains. Next, we use various <italic>in silico</italic> tools to predict consensus immunogenic and conserved peptide. We found that conserved region is present only in the RNA directed RNA polymerase protein. In this protein we identified one epitope WDYPKCDRA is highly immunogenic and 100% conserved among all available human coronavirus strains.</p></sec><sec><title>Conclusions</title><p>Here we suggest <italic>in vivo</italic> study of our identified novel peptide antigen in RNA directed RNA polymerase protein for universal vaccine – which may be the way to prevent all human coronavirus disease.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1471-2105-15-161) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Luis, E" uniqKey="Luis E">E Luis</name></author><author><name sortKey="Isabel, S" uniqKey="Isabel S">S Isabel</name></author><author><name sortKey="Sonia, Z" uniqKey="Sonia Z">Z Sonia</name></author><author><name sortKey="Fernando, A" uniqKey="Fernando A">A Fernando</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Groot, Rj" uniqKey="De Groot R">RJ De Groot</name></author><author><name sortKey="Baker, Sc" uniqKey="Baker S">SC Baker</name></author><author><name sortKey="Baric, R" uniqKey="Baric R">R Baric</name></author><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author><author><name sortKey="Gorbalenya, Ae" uniqKey="Gorbalenya A">AE Gorbalenya</name></author><author><name sortKey="Holmes, Kv" uniqKey="Holmes K">KV Holmes</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S Perlman</name></author><author><name sortKey="Poon, L" uniqKey="Poon L">L Poon</name></author><author><name sortKey="Rottier, Pjm" uniqKey="Rottier P">PJM Rottier</name></author><author><name sortKey="Talbot, Pj" uniqKey="Talbot P">PJ Talbot</name></author><author><name sortKey="Woo, Pcy" uniqKey="Woo P">PCY Woo</name></author><author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J Ziebuhr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, R" uniqKey="Lu R">R Lu</name></author><author><name sortKey="Yu, X" uniqKey="Yu X">X Yu</name></author><author><name sortKey="Wang, W" uniqKey="Wang W">W Wang</name></author><author><name sortKey="Duan, X" uniqKey="Duan X">X Duan</name></author><author><name sortKey="Zhang, L" uniqKey="Zhang L">L Zhang</name></author><author><name sortKey="Roujian, L" uniqKey="Roujian L">L Roujian</name></author><author><name sortKey="Xiaoyan, Y" uniqKey="Xiaoyan Y">Y Xiaoyan</name></author><author><name sortKey="Wen, W" uniqKey="Wen W">W Wen</name></author><author><name sortKey="Xijie, D" uniqKey="Xijie D">D Xijie</name></author><author><name sortKey="Linglin, Z" uniqKey="Linglin Z">Z Linglin</name></author><author><name sortKey="Weimin, Z" uniqKey="Weimin Z">Z Weimin</name></author><author><name sortKey="Jin, X" uniqKey="Jin X">X Jin</name></author><author><name sortKey="Lingjie, X" uniqKey="Lingjie X">X Lingjie</name></author><author><name sortKey="Qin, H" uniqKey="Qin H">H Qin</name></author><author><name sortKey="Jianxin, L" uniqKey="Jianxin L">L Jianxin</name></author><author><name sortKey="Li, R" uniqKey="Li R">R Li</name></author><author><name sortKey="Zhong, W" uniqKey="Zhong W">W Zhong</name></author><author><name sortKey="Wenjie, T" uniqKey="Wenjie T">T Wenjie</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Boheemen, S" uniqKey="Boheemen S">S Boheemen</name></author><author><name sortKey="Sander, B" uniqKey="Sander B">B Sander</name></author><author><name sortKey="Miranda, G" uniqKey="Miranda G">G Miranda</name></author><author><name sortKey="Chris, L" uniqKey="Chris L">L Chris</name></author><author><name sortKey="Theo, Mb" uniqKey="Theo M">MB Theo</name></author><author><name sortKey="Stalin, R" uniqKey="Stalin R">R Stalin</name></author><author><name sortKey="Ali, Mz" uniqKey="Ali M">MZ Ali</name></author><author><name sortKey="Albert, Dme" uniqKey="Albert D">DME Albert</name></author><author><name sortKey="Bart, Lh" uniqKey="Bart L">LH Bart</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Alexander, Eg" uniqKey="Alexander E">EG Alexander</name></author><author><name sortKey="Eric, Js" uniqKey="Eric J">JS Eric</name></author><author><name sortKey="Ron, Amf" uniqKey="Ron A">AMF Ron</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wever, Ba" uniqKey="Wever B">BA Wever</name></author><author><name sortKey="Van Der Hoek, L" uniqKey="Van Der Hoek L">L Van der Hoek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan Yeung, M" uniqKey="Chan Yeung M">M Chan-Yeung</name></author><author><name sortKey="Xu, Rh" uniqKey="Xu R">RH Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hofmann, H" uniqKey="Hofmann H">H Hofmann</name></author><author><name sortKey="Pyrc, K" uniqKey="Pyrc K">K Pyrc</name></author><author><name sortKey="Van Der Hoek, L" uniqKey="Van Der Hoek L">L Van der Hoek</name></author><author><name sortKey="Geier, M" uniqKey="Geier M">M Geier</name></author><author><name sortKey="Ben, B" uniqKey="Ben B">B Ben</name></author><author><name sortKey="Stefan, P" uniqKey="Stefan P">P Stefan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ali Mohamed, Z" uniqKey="Ali Mohamed Z">Z Ali Mohamed</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S Van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Albert, Dme" uniqKey="Albert D">DME Albert</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Ron, Am" uniqKey="Ron A">AM Ron</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="S Rensen, Md" uniqKey="S Rensen M">MD Sørensen</name></author><author><name sortKey="S Rensen, B" uniqKey="S Rensen B">B Sørensen</name></author><author><name sortKey="Gonzalez Dosal, R" uniqKey="Gonzalez Dosal R">R Gonzalez-Dosal</name></author><author><name sortKey="Melchjorsen, Cj" uniqKey="Melchjorsen C">CJ Melchjorsen</name></author><author><name sortKey="Weibel, J" uniqKey="Weibel J">J Weibel</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J Wang</name></author><author><name sortKey="Jun, Cw" uniqKey="Jun C">CW Jun</name></author><author><name sortKey="Huanming, Y" uniqKey="Huanming Y">Y Huanming</name></author><author><name sortKey="Kristensen, P" uniqKey="Kristensen P">P Kristensen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheng, Vc" uniqKey="Cheng V">VC Cheng</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pierre, S" uniqKey="Pierre S">S Pierre</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Robert, R" uniqKey="Robert R">R Robert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Helen, B" uniqKey="Helen B">B Helen</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Falzarano, D" uniqKey="Falzarano D">D Falzarano</name></author><author><name sortKey="Darryl, F" uniqKey="Darryl F">F Darryl</name></author><author><name sortKey="De Emmie, W" uniqKey="De Emmie W">W De Emmie</name></author><author><name sortKey="Cynthia, M" uniqKey="Cynthia M">M Cynthia</name></author><author><name sortKey="Julie, C" uniqKey="Julie C">C Julie</name></author><author><name sortKey="Vincent, J" uniqKey="Vincent J">J Vincent</name></author><author><name sortKey="Heinz, F" uniqKey="Heinz F">F Heinz</name></author><author><name sortKey="Munster, Vj" uniqKey="Munster V">VJ Munster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wong, Ss" uniqKey="Wong S">SS Wong</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Xianfu, W" uniqKey="Xianfu W">W Xianfu</name></author><author><name sortKey="Smith, Tg" uniqKey="Smith T">TG Smith</name></author><author><name sortKey="Rupprecht, Ce" uniqKey="Rupprecht C">CE Rupprecht</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Plotkin, Sa" uniqKey="Plotkin S">SA Plotkin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matthew, C" uniqKey="Matthew C">C Matthew</name></author><author><name sortKey="Lam, Tt" uniqKey="Lam T">TT Lam</name></author><author><name sortKey="Watson, Sj" uniqKey="Watson S">SJ Watson</name></author><author><name sortKey="Annie, Lp" uniqKey="Annie L">LP Annie</name></author><author><name sortKey="Velilava, P" uniqKey="Velilava P">P Velilava</name></author><author><name sortKey="Paul, G" uniqKey="Paul G">G Paul</name></author><author><name sortKey="Oliver, Gp" uniqKey="Oliver G">GP Oliver</name></author><author><name sortKey="Andrew, R" uniqKey="Andrew R">R Andrew</name></author><author><name sortKey="Yi, G" uniqKey="Yi G">G Yi</name></author><author><name sortKey="Deenan, P" uniqKey="Deenan P">P Deenan</name></author><author><name sortKey="Paul, K" uniqKey="Paul K">K Paul</name></author><author><name sortKey="Eleni, K" uniqKey="Eleni K">K Eleni</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Islam, R" uniqKey="Islam R">R Islam</name></author><author><name sortKey="Sadman, S" uniqKey="Sadman S">S Sadman</name></author><author><name sortKey="Aubhishek, Z" uniqKey="Aubhishek Z">Z Aubhishek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thompson, Jd" uniqKey="Thompson J">JD Thompson</name></author><author><name sortKey="Higgins, Dg" uniqKey="Higgins D">DG Higgins</name></author><author><name sortKey="Gibson, Tj" uniqKey="Gibson T">TJ Gibson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Boronat, M" uniqKey="Garcia Boronat M">M Garcia-Boronat</name></author><author><name sortKey="Diez Rivero, Cm" uniqKey="Diez Rivero C">CM Diez-Rivero</name></author><author><name sortKey="Reinherz, El" uniqKey="Reinherz E">EL Reinherz</name></author><author><name sortKey="Pedro, Ar" uniqKey="Pedro A">AR Pedro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Waterhouse, Am" uniqKey="Waterhouse A">AM Waterhouse</name></author><author><name sortKey="Procter, Jb" uniqKey="Procter J">JB Procter</name></author><author><name sortKey="Martin, Dm" uniqKey="Martin D">DM Martin</name></author><author><name sortKey="Clamp, M" uniqKey="Clamp M">M Clamp</name></author><author><name sortKey="Barton, Gj" uniqKey="Barton G">GJ Barton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Caoili, Sec" uniqKey="Caoili S">SEC Caoili</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kolaskar, A" uniqKey="Kolaskar A">A Kolaskar</name></author><author><name sortKey="Tongaonkar, Pc" uniqKey="Tongaonkar P">PC Tongaonkar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vita, R" uniqKey="Vita R">R Vita</name></author><author><name sortKey="Zarebski, L" uniqKey="Zarebski L">L Zarebski</name></author><author><name sortKey="Greenbaum, Ja" uniqKey="Greenbaum J">JA Greenbaum</name></author><author><name sortKey="Emami, H" uniqKey="Emami H">H Emami</name></author><author><name sortKey="Hoof, I" uniqKey="Hoof I">I Hoof</name></author><author><name sortKey="Salimi, N" uniqKey="Salimi N">N Salimi</name></author><author><name sortKey="Damle, R" uniqKey="Damle R">R Damle</name></author><author><name sortKey="Sette, A" uniqKey="Sette A">A Sette</name></author><author><name sortKey="Peters, B" uniqKey="Peters B">B Peters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jens Erik, Pl" uniqKey="Jens Erik P">PL Jens Erik</name></author><author><name sortKey="Pontoppidan, L" uniqKey="Pontoppidan L">L Pontoppidan</name></author><author><name sortKey="Ole, L" uniqKey="Ole L">L Ole</name></author><author><name sortKey="Morten, N" uniqKey="Morten N">N Morten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Emini, Ea" uniqKey="Emini E">EA Emini</name></author><author><name sortKey="Hughes, Jv" uniqKey="Hughes J">JV Hughes</name></author><author><name sortKey="Perlow, Ds" uniqKey="Perlow D">DS Perlow</name></author><author><name sortKey="Boger, J" uniqKey="Boger J">J Boger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bui, Hh" uniqKey="Bui H">HH Bui</name></author><author><name sortKey="Sidney, J" uniqKey="Sidney J">J Sidney</name></author><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author><author><name sortKey="Fusseder, N" uniqKey="Fusseder N">N Fusseder</name></author><author><name sortKey="Sette, A" uniqKey="Sette A">A Sette</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parker, Jm" uniqKey="Parker J">JM Parker</name></author><author><name sortKey="Guo, D" uniqKey="Guo D">D Guo</name></author><author><name sortKey="Hodges, Rs" uniqKey="Hodges R">RS Hodges</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laskowski, Ra" uniqKey="Laskowski R">RA Laskowski</name></author><author><name sortKey="Macarthur, Mw" uniqKey="Macarthur M">MW MacArthur</name></author><author><name sortKey="Moss, Ds" uniqKey="Moss D">DS Moss</name></author><author><name sortKey="Thornton, Jm" uniqKey="Thornton J">JM Thornton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pettersen, Ef" uniqKey="Pettersen E">EF Pettersen</name></author><author><name sortKey="Goddard, Td" uniqKey="Goddard T">TD Goddard</name></author><author><name sortKey="Huang, Cc" uniqKey="Huang C">CC Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="To, Kkw" uniqKey="To K">KKW To</name></author><author><name sortKey="Hung, Ifn" uniqKey="Hung I">IFN Hung</name></author><author><name sortKey="Chan, Jfw" uniqKey="Chan J">JFW Chan</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Muller</name></author><author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Muller</name></author><author><name sortKey="Stalin, Rv" uniqKey="Stalin R">RV Stalin</name></author><author><name sortKey="Doreen, M" uniqKey="Doreen M">M Doreen</name></author><author><name sortKey="Benjamin, M" uniqKey="Benjamin M">M Benjamin</name></author><author><name sortKey="Stephan, K" uniqKey="Stephan K">K Stephan</name></author><author><name sortKey="Saskia, L" uniqKey="Saskia L">L Saskia</name></author><author><name sortKey="Robert, W" uniqKey="Robert W">W Robert</name></author><author><name sortKey="Sabine, S" uniqKey="Sabine S">S Sabine</name></author><author><name sortKey="Tasnim, S" uniqKey="Tasnim S">S Tasnim</name></author><author><name sortKey="Katrin, Z" uniqKey="Katrin Z">Z Katrin</name></author><author><name sortKey="Tabea, B" uniqKey="Tabea B">B Tabea</name></author><author><name sortKey="Isabella, E" uniqKey="Isabella E">E Isabella</name></author><author><name sortKey="Marco, T" uniqKey="Marco T">T Marco</name></author><author><name sortKey="Ali, Mz" uniqKey="Ali M">MZ Ali</name></author><author><name sortKey="Albert, Dme" uniqKey="Albert D">DME Albert</name></author><author><name sortKey="Ron, Am" uniqKey="Ron A">AM Ron</name></author><author><name sortKey="Bart, Lh" uniqKey="Bart L">LH Bart</name></author><author><name sortKey="Christian, D" uniqKey="Christian D">D Christian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Zy" uniqKey="Yang Z">ZY Yang</name></author><author><name sortKey="Kong, Wp" uniqKey="Kong W">WP Kong</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Roberts, A" uniqKey="Roberts A">A Roberts</name></author><author><name sortKey="Murphy, Br" uniqKey="Murphy B">BR Murphy</name></author><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K Subbarao</name></author><author><name sortKey="Nabel, Gj" uniqKey="Nabel G">GJ Nabel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sudhakar, A" uniqKey="Sudhakar A">A Sudhakar</name></author><author><name sortKey="Robin, G" uniqKey="Robin G">G Robin</name></author><author><name sortKey="Boyd, L" uniqKey="Boyd L">L Boyd</name></author><author><name sortKey="Agnihothram, S" uniqKey="Agnihothram S">S Agnihothram</name></author><author><name sortKey="Gopal, R" uniqKey="Gopal R">R Gopal</name></author><author><name sortKey="Yount, Bl" uniqKey="Yount B">BL Yount</name></author><author><name sortKey="Donaldson, Ef" uniqKey="Donaldson E">EF Donaldson</name></author><author><name sortKey="Menachery, Vd" uniqKey="Menachery V">VD Menachery</name></author><author><name sortKey="Graham, Rl" uniqKey="Graham R">RL Graham</name></author><author><name sortKey="Scobey, Td" uniqKey="Scobey T">TD Scobey</name></author><author><name sortKey="Gralinski, Le" uniqKey="Gralinski L">LE Gralinski</name></author><author><name sortKey="Denison, Mr" uniqKey="Denison M">MR Denison</name></author><author><name sortKey="Zambon, M" uniqKey="Zambon M">M Zambon</name></author><author><name sortKey="Baric, R" uniqKey="Baric R">R Baric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fernando, A" uniqKey="Fernando A">A Fernando</name></author><author><name sortKey="Marta, L" uniqKey="Marta L">L Marta</name></author><author><name sortKey="Isabel, S" uniqKey="Isabel S">S Isabel</name></author><author><name sortKey="Sonia, Z" uniqKey="Sonia Z">Z Sonia</name></author><author><name sortKey="Jose, L" uniqKey="Jose L">L Jose</name></author><author><name sortKey="Silvia, Lj" uniqKey="Silvia L">LJ Silvia</name></author><author><name sortKey="German, A" uniqKey="German A">A German</name></author><author><name sortKey="Luis, E" uniqKey="Luis E">E Luis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pica, N" uniqKey="Pica N">N Pica</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Elaine, Vj" uniqKey="Elaine V">VJ Elaine</name></author><author><name sortKey="Sharon, K" uniqKey="Sharon K">K Sharon</name></author><author><name sortKey="Timothy, Jm" uniqKey="Timothy J">JM Timothy</name></author><author><name sortKey="Albert, Pr" uniqKey="Albert P">PR Albert</name></author><author><name sortKey="Smithkline, Bc" uniqKey="Smithkline B">BC Smithkline</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arnon, R" uniqKey="Arnon R">R Arnon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Olesen, Of" uniqKey="Olesen O">OF Olesen</name></author><author><name sortKey="Lonnoroth, A" uniqKey="Lonnoroth A">A Lonnoroth</name></author><author><name sortKey="Mulligan, B" uniqKey="Mulligan B">B Mulligan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Benson, Da" uniqKey="Benson D">DA Benson</name></author><author><name sortKey="Cavanaugh, M" uniqKey="Cavanaugh M">M Cavanaugh</name></author><author><name sortKey="Clark, K" uniqKey="Clark K">K Clark</name></author><author><name sortKey="Karsch Mizrachi, I" uniqKey="Karsch Mizrachi I">I Karsch-Mizrachi</name></author><author><name sortKey="Lipman, Dj" uniqKey="Lipman D">DJ Lipman</name></author><author><name sortKey="Ostell, J" uniqKey="Ostell J">J Ostell</name></author><author><name sortKey="Sayers, Ew" uniqKey="Sayers E">EW Sayers</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Bioinformatics</journal-id><journal-id journal-id-type="iso-abbrev">BMC Bioinformatics</journal-id><journal-title-group><journal-title>BMC Bioinformatics</journal-title></journal-title-group><issn pub-type="epub">1471-2105</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24884408</article-id><article-id pub-id-type="pmc">4041900</article-id><article-id pub-id-type="publisher-id">6430</article-id><article-id pub-id-type="doi">10.1186/1471-2105-15-161</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>A highly conserved WDYPKCDRA epitope in the RNA directed RNA polymerase of human coronaviruses can be used as epitope-based universal vaccine design</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Sharmin</surname><given-names>Refat</given-names></name><address><email>refat519@gmail.com</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Islam</surname><given-names>Abul Bashar Mir Md Khademul</given-names></name><address><email>khademul@du.ac.bd</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.8198.8</institution-id><institution-id institution-id-type="ISNI">0000000114986059</institution-id><institution>Department of Genetic Engineering and Biotechnology,</institution><institution>University of Dhaka, Science Complex Building,</institution></institution-wrap>Dhaka, 1000 Bangladesh</aff></contrib-group><pub-date pub-type="epub"><day>29</day><month>5</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>29</day><month>5</month><year>2014</year></pub-date><pub-date pub-type="collection"><year>2014</year></pub-date><volume>15</volume><elocation-id>161</elocation-id><history><date date-type="received"><day>12</day><month>2</month><year>2014</year></date><date date-type="accepted"><day>19</day><month>5</month><year>2014</year></date></history><permissions><copyright-statement>© Sharmin and Islam; licensee BioMed Central Ltd. 2014</copyright-statement><license license-type="OpenAccess"><license-p>This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0">http://creativecommons.org/licenses/by/4.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p>Coronaviruses are the diverse group of RNA virus. From 1960, six strains of human coronaviruses have emerged that includes SARS-CoV and the recent infection by deadly MERS-CoV which is now going to cause another outbreak. Prevention of these viruses is urgent and a universal vaccine for all strain could be a promising solution in this circumstance. In this study we aimed to design an epitope based vaccine against all strain of human coronavirus.</p></sec><sec><title>Results</title><p>Multiple sequence alignment (MSA) approach was employed among spike (S), membrane (M), enveloped (E) and nucleocapsid (N) protein and replicase polyprotein 1ab to identify which one is highly conserve in all coronaviruses strains. Next, we use various <italic>in silico</italic> tools to predict consensus immunogenic and conserved peptide. We found that conserved region is present only in the RNA directed RNA polymerase protein. In this protein we identified one epitope WDYPKCDRA is highly immunogenic and 100% conserved among all available human coronavirus strains.</p></sec><sec><title>Conclusions</title><p>Here we suggest <italic>in vivo</italic> study of our identified novel peptide antigen in RNA directed RNA polymerase protein for universal vaccine – which may be the way to prevent all human coronavirus disease.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1471-2105-15-161) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Coronavirus</kwd><kwd>SARS-CoV</kwd><kwd>MERS-CoV</kwd><kwd>RNA directed RNA polymerase</kwd><kwd>Epitope</kwd><kwd>Universal vaccine</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2014</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000191 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000191 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:4041900
|texte= A highly conserved WDYPKCDRA epitope in the RNA directed RNA polymerase of human coronaviruses can be used as epitope-based universal vaccine design
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:24884408" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a CovidV2
| This area was generated with Dilib version V0.6.33. |  |