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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis</title><author><name sortKey="Zaghloul, Hosam" sort="Zaghloul, Hosam" uniqKey="Zaghloul H" first="Hosam" last="Zaghloul">Hosam Zaghloul</name></author><author><name sortKey="El Shahat, Mahmoud" sort="El Shahat, Mahmoud" uniqKey="El Shahat M" first="Mahmoud" last="El-Shahat">Mahmoud El-Shahat</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25544878</idno><idno type="pmc">4269910</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269910</idno><idno type="RBID">PMC:4269910</idno><idno type="doi">10.4254/wjh.v6.i12.916</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000A68</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000A68</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis</title><author><name sortKey="Zaghloul, Hosam" sort="Zaghloul, Hosam" uniqKey="Zaghloul H" first="Hosam" last="Zaghloul">Hosam Zaghloul</name></author><author><name sortKey="El Shahat, Mahmoud" sort="El Shahat, Mahmoud" uniqKey="El Shahat M" first="Mahmoud" last="El-Shahat">Mahmoud El-Shahat</name></author></analytic><series><title level="j">World Journal of Hepatology</title><idno type="ISSN">1948-5182</idno><idno type="eISSN">1948-5182</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Hepatitis C virus (HCV) infection represents a significant health problem and represents a heavy load on some countries like Egypt in which about 20% of the total population are infected. Initial infection is usually asymptomatic and result in chronic hepatitis that give rise to complications including cirrhosis and hepatocellular carcinoma. The management of HCV infection should not only be focus on therapy, but also to screen carrier individuals in order to prevent transmission. In the present, molecular detection and quantification of HCV genome by real time polymerase chain reaction (PCR) represent the gold standard in HCV diagnosis and plays a crucial role in the management of therapeutic regimens. However, real time PCR is a complicated approach and of limited distribution. On the other hand, isothermal DNA amplification techniques have been developed and offer molecular diagnosis of infectious dieses at point-of-care. In this review we discuss recombinase polymerase amplification technique and illustrate its diagnostic value over both PCR and other isothermal amplification techniques.</p></div></front></TEI><pmc article-type="review-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">World J Hepatol</journal-id><journal-id journal-id-type="publisher-id">WJH</journal-id><journal-title-group><journal-title>World Journal of Hepatology</journal-title></journal-title-group><issn pub-type="ppub">1948-5182</issn><issn pub-type="epub">1948-5182</issn><publisher><publisher-name>Baishideng Publishing Group Inc</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25544878</article-id><article-id pub-id-type="pmc">4269910</article-id><article-id pub-id-type="other">jWJH.v6.i12.pg916</article-id><article-id pub-id-type="doi">10.4254/wjh.v6.i12.916</article-id><article-categories><subj-group subj-group-type="heading"><subject>Minireviews</subject></subj-group></article-categories><title-group><article-title>Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zaghloul</surname><given-names>Hosam</given-names></name></contrib><contrib contrib-type="author"><name><surname>El-shahat</surname><given-names>Mahmoud</given-names></name></contrib><aff>Hosam Zaghloul, Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Al Daqahliyah 35514-60, Egypt</aff><aff>Mahmoud El-shahat, Zoology Department, Faculty of Science, Mansoura University, Mansoura, Al Daqahliyah 35514-60, Egypt</aff></contrib-group><author-notes><fn><p>Author contributions: Zaghloul H designed and revised the review; El-shahat M collected information and draft the review.</p><p>Correspondence to: Hosam Zaghloul, Professor, Department of Clinical Pathology, Faculty of Medicine, Mansoura University, El Gomhoria Street, Mansoura, Al Daqahliyah 35514-60, Egypt. <email>hosam_z@yahoo.com</email></p><p>Telephone: +20-50-2361696 Fax: +20-50-2351797</p></fn></author-notes><pub-date pub-type="ppub"><day>27</day><month>12</month><year>2014</year></pub-date><pub-date pub-type="epub"><day>27</day><month>12</month><year>2014</year></pub-date><volume>6</volume><issue>12</issue><fpage>916</fpage><lpage>922</lpage><history><date date-type="received"><day>25</day><month>8</month><year>2014</year></date><date date-type="rev-recd"><day>13</day><month>10</month><year>2014</year></date><date date-type="accepted"><day>28</day><month>10</month><year>2014</year></date></history><permissions><copyright-statement>©2014 Baishideng Publishing Group Inc. All rights reserved.</copyright-statement><copyright-year>2014</copyright-year></permissions><abstract><p>Hepatitis C virus (HCV) infection represents a significant health problem and represents a heavy load on some countries like Egypt in which about 20% of the total population are infected. Initial infection is usually asymptomatic and result in chronic hepatitis that give rise to complications including cirrhosis and hepatocellular carcinoma. The management of HCV infection should not only be focus on therapy, but also to screen carrier individuals in order to prevent transmission. In the present, molecular detection and quantification of HCV genome by real time polymerase chain reaction (PCR) represent the gold standard in HCV diagnosis and plays a crucial role in the management of therapeutic regimens. However, real time PCR is a complicated approach and of limited distribution. On the other hand, isothermal DNA amplification techniques have been developed and offer molecular diagnosis of infectious dieses at point-of-care. In this review we discuss recombinase polymerase amplification technique and illustrate its diagnostic value over both PCR and other isothermal amplification techniques.</p></abstract><kwd-group><kwd>Hepatitis C virus</kwd><kwd>Nucleic acid testing</kwd><kwd>Polymerase chain reaction</kwd><kwd>Point-of-care</kwd><kwd>Recombinase polymerase amplification</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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