Interleukin-18 expression and the response to treatment in patients with psoriasis
Identifieur interne : 000810 ( Pmc/Corpus ); précédent : 000809; suivant : 000811Interleukin-18 expression and the response to treatment in patients with psoriasis
Auteurs : Hanaa Rasmy ; Nancy Mikhael ; Somaia IsmailSource :
- Archives of Medical Science : AMS [ 1734-1922 ] ; 2011.
Abstract
The aim of the study was to demonstrate interleukin-18 (IL-18) expression in keratinocytes from psoriatic lesions in comparison to keratinocytes from uninvolved skin and to study the change of expression after therapeutic interventions.
This study included 16 patients of different clinical subtypes of psoriasis. Interleukin-18 gene expression analysis was performed using real time quantitative PCR. Three biopsies were obtained from each patient. Two were taken from the lesional psoriatic skin and from uninvolved skin before starting treatment. A third lesional skin biopsy was taken at the end of 2 months of treatment. The treatment was in the form of topical steroids or oral systemic methotrexate.
Of all 16 studied patients, significantly increased IL-18 expression was noted in keratinocytes from psoriatic lesions before and after treatment when compared to keratinocytes from uninvolved skin (
Increased IL-18 expression in keratinocytes from psoriatic lesions of our patients and its correlation with disease duration and severity supported the concept of psoriasis as a T cell mediated autoimmune disease. This could establish therapeutic and preventive approaches for psoriasis that ultimately lead to improved outcomes for patients.
Url:
DOI: 10.5114/aoms.2011.24144
PubMed: 22291810
PubMed Central: 3258774
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Interleukin-18 expression and the response to treatment in patients with psoriasis</title><author><name sortKey="Rasmy, Hanaa" sort="Rasmy, Hanaa" uniqKey="Rasmy H" first="Hanaa" last="Rasmy">Hanaa Rasmy</name><affiliation><nlm:aff id="aff1">National Research Center, Cairo, Egypt</nlm:aff></affiliation></author><author><name sortKey="Mikhael, Nancy" sort="Mikhael, Nancy" uniqKey="Mikhael N" first="Nancy" last="Mikhael">Nancy Mikhael</name><affiliation><nlm:aff id="aff2">Benha Faculty of Medicine, Benha, Egypt</nlm:aff></affiliation></author><author><name sortKey="Ismail, Somaia" sort="Ismail, Somaia" uniqKey="Ismail S" first="Somaia" last="Ismail">Somaia Ismail</name><affiliation><nlm:aff id="aff1">National Research Center, Cairo, Egypt</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22291810</idno><idno type="pmc">3258774</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258774</idno><idno type="RBID">PMC:3258774</idno><idno type="doi">10.5114/aoms.2011.24144</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">000810</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000810</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Interleukin-18 expression and the response to treatment in patients with psoriasis</title><author><name sortKey="Rasmy, Hanaa" sort="Rasmy, Hanaa" uniqKey="Rasmy H" first="Hanaa" last="Rasmy">Hanaa Rasmy</name><affiliation><nlm:aff id="aff1">National Research Center, Cairo, Egypt</nlm:aff></affiliation></author><author><name sortKey="Mikhael, Nancy" sort="Mikhael, Nancy" uniqKey="Mikhael N" first="Nancy" last="Mikhael">Nancy Mikhael</name><affiliation><nlm:aff id="aff2">Benha Faculty of Medicine, Benha, Egypt</nlm:aff></affiliation></author><author><name sortKey="Ismail, Somaia" sort="Ismail, Somaia" uniqKey="Ismail S" first="Somaia" last="Ismail">Somaia Ismail</name><affiliation><nlm:aff id="aff1">National Research Center, Cairo, Egypt</nlm:aff></affiliation></author></analytic><series><title level="j">Archives of Medical Science : AMS</title><idno type="ISSN">1734-1922</idno><idno type="eISSN">1896-9151</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Introduction</title><p>The aim of the study was to demonstrate interleukin-18 (IL-18) expression in keratinocytes from psoriatic lesions in comparison to keratinocytes from uninvolved skin and to study the change of expression after therapeutic interventions.</p></sec><sec><title>Material and methods</title><p>This study included 16 patients of different clinical subtypes of psoriasis. Interleukin-18 gene expression analysis was performed using real time quantitative PCR. Three biopsies were obtained from each patient. Two were taken from the lesional psoriatic skin and from uninvolved skin before starting treatment. A third lesional skin biopsy was taken at the end of 2 months of treatment. The treatment was in the form of topical steroids or oral systemic methotrexate.</p></sec><sec><title>Results</title><p>Of all 16 studied patients, significantly increased IL-18 expression was noted in keratinocytes from psoriatic lesions before and after treatment when compared to keratinocytes from uninvolved skin (<italic>p</italic> = 0.001 and <italic>p</italic> = 0.002 respectively). The IL-18 expression in the skin lesions after treatment was significantly lower than lesional skin before treatment (<italic>p</italic> = 0.023). In psoriatic skin lesions of all studied patients IL-18 expression was significantly correlated with disease duration (<italic>r</italic> = 0.40 and <italic>p</italic> = 0.01) and clinical severity of psoriasis (<italic>r</italic> = 0.72 and <italic>p</italic> = 0.001).</p></sec><sec><title>Conclusions</title><p>Increased IL-18 expression in keratinocytes from psoriatic lesions of our patients and its correlation with disease duration and severity supported the concept of psoriasis as a T cell mediated autoimmune disease. This could establish therapeutic and preventive approaches for psoriasis that ultimately lead to improved outcomes for patients.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Homey, B" uniqKey="Homey B">B Homey</name></author><author><name sortKey="Dieu Nosjean, Mc" uniqKey="Dieu Nosjean M">MC Dieu-Nosjean</name></author><author><name sortKey="Wiesenborn, A" uniqKey="Wiesenborn A">A Wiesenborn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Krueger, Jg" uniqKey="Krueger J">JG Krueger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valdimarsson, H" uniqKey="Valdimarsson H">H Valdimarsson</name></author><author><name sortKey="Baker, Bs" uniqKey="Baker B">BS Baker</name></author><author><name sortKey="Jonsdottir, I" uniqKey="Jonsdottir I">I Jonsdottir</name></author><author><name sortKey="Fry, L" uniqKey="Fry L">L 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Arch Med Sci</journal-id><journal-id journal-id-type="publisher-id">AMS</journal-id><journal-title-group><journal-title>Archives of Medical Science : AMS</journal-title></journal-title-group><issn pub-type="ppub">1734-1922</issn><issn pub-type="epub">1896-9151</issn><publisher><publisher-name>Termedia Publishing House</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">22291810</article-id><article-id pub-id-type="pmc">3258774</article-id><article-id pub-id-type="publisher-id">AMS-7-4-713</article-id><article-id pub-id-type="doi">10.5114/aoms.2011.24144</article-id><article-categories><subj-group subj-group-type="heading"><subject>Clinical Research</subject></subj-group></article-categories><title-group><article-title>Interleukin-18 expression and the response to treatment in patients with psoriasis</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Rasmy</surname><given-names>Hanaa</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Mikhael</surname><given-names>Nancy</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Ismail</surname><given-names>Somaia</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib></contrib-group><aff id="aff1"><label>1</label>National Research Center, Cairo, Egypt</aff><aff id="aff2"><label>2</label>Benha Faculty of Medicine, Benha, Egypt</aff><author-notes><corresp id="cor1"><bold>Corresponding author:</bold> Dr. Hanaa Rasmy, Mohamed Attia, Clinical and Chemical, Pathology Department, Medical Division National Research Center, 19 Ahmed Fahim, Nasr City, Cairo, Egypt, Phone: +2 012 22 080 47. E-mail: <email xlink:href="Hanarasmy2000@yahoo.com">Hanarasmy2000@yahoo.com</email></corresp></author-notes><pub-date pub-type="ppub"><month>8</month><year>2011</year></pub-date><pub-date pub-type="epub"><day>2</day><month>9</month><year>2011</year></pub-date><volume>7</volume><issue>4</issue><fpage>713</fpage><lpage>719</lpage><history><date date-type="received"><day>18</day><month>8</month><year>2009</year></date><date date-type="rev-recd"><day>15</day><month>11</month><year>2009</year></date><date date-type="accepted"><day>22</day><month>2</month><year>2010</year></date></history><permissions><copyright-statement>Copyright © 2011 Termedia & Banach</copyright-statement><copyright-year>2011</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-nd/3.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><sec><title>Introduction</title><p>The aim of the study was to demonstrate interleukin-18 (IL-18) expression in keratinocytes from psoriatic lesions in comparison to keratinocytes from uninvolved skin and to study the change of expression after therapeutic interventions.</p></sec><sec><title>Material and methods</title><p>This study included 16 patients of different clinical subtypes of psoriasis. Interleukin-18 gene expression analysis was performed using real time quantitative PCR. Three biopsies were obtained from each patient. Two were taken from the lesional psoriatic skin and from uninvolved skin before starting treatment. A third lesional skin biopsy was taken at the end of 2 months of treatment. The treatment was in the form of topical steroids or oral systemic methotrexate.</p></sec><sec><title>Results</title><p>Of all 16 studied patients, significantly increased IL-18 expression was noted in keratinocytes from psoriatic lesions before and after treatment when compared to keratinocytes from uninvolved skin (<italic>p</italic> = 0.001 and <italic>p</italic> = 0.002 respectively). The IL-18 expression in the skin lesions after treatment was significantly lower than lesional skin before treatment (<italic>p</italic> = 0.023). In psoriatic skin lesions of all studied patients IL-18 expression was significantly correlated with disease duration (<italic>r</italic> = 0.40 and <italic>p</italic> = 0.01) and clinical severity of psoriasis (<italic>r</italic> = 0.72 and <italic>p</italic> = 0.001).</p></sec><sec><title>Conclusions</title><p>Increased IL-18 expression in keratinocytes from psoriatic lesions of our patients and its correlation with disease duration and severity supported the concept of psoriasis as a T cell mediated autoimmune disease. This could establish therapeutic and preventive approaches for psoriasis that ultimately lead to improved outcomes for patients.</p></sec></abstract><kwd-group><kwd>psoriasis</kwd><kwd>interleukins</kwd><kwd>real-time PCR</kwd><kwd>effect of therapy</kwd></kwd-group></article-meta></front><body><sec><title>Introduction</title><p>Psoriasis is a chronic inflammatory condition which is the result of persistent stimulation of T cells by antigens of epidermal origin [<xref ref-type="bibr" rid="R1">1</xref>]. This persistent stimulation of T cells is the result of interaction between T cells, antigen-presenting cells, i.e. Langerhans cells, and antigens, and comprises the following steps: primary signals (signal 1) including T-cell receptor stimulation by peptide antigen, costimulation (so-called accessory signals) (signal 2) and T helper type 1 (Th1) differentiation and proliferation [<xref ref-type="bibr" rid="R2">2</xref>].</p><p>In 1986, it was proposed that cytokines released by activated T-lymphocytes initiate and maintain the psoriatic process by stimulating keratinocyte proliferation [<xref ref-type="bibr" rid="R3">3</xref>]. Since that time it has become clear that, upon stimulation, keratinocytes are also able to secrete an array of different cytokines with a variety of functional effects on both themselves and other cell types, including T-lymphocytes [<xref ref-type="bibr" rid="R4">4</xref>].</p><p>Interleukin (IL)-18 is related to the IL-1 family in terms of both structure and function. At the receptor level, the activity of IL-18 takes place through a signalling chain of a putative IL-18 receptor (IL-18R) complex [<xref ref-type="bibr" rid="R5">5</xref>].</p><p>Interleukin-18 was first described as an interferon-γ (IFN-γ) inducing factor. In general, IL-18 induction of IFN-γ is similar to that of IL-12, when it is a sole cytokine. Interleukin-18 induces low levels of IFN-γ, but in the presence of costimulants, IFN-γ production is greatly enhanced [<xref ref-type="bibr" rid="R6">6</xref>]. However, because antibodies to IL-18 also reduced the hepatotoxicity of endotoxaemia, IL-18 was considered to possess other biological properties beyond that of inducing IFN-γ [<xref ref-type="bibr" rid="R7">7</xref>]. Interleukin-18 activates T cells to synthesize IL-2, GM-CSF, and TNF-α. There are also reports that IL-18 suppresses the production of IL-10 and does not induce the production of IL-1Ra. In general, the ability of IL-18 to induce different cytokines depends on the cellular targets [<xref ref-type="bibr" rid="R8">8</xref>]. Flisiak <italic>et al.</italic> confirmed an association between plasma IL-18 concentration and psoriasis severity. Moreover, it was shown that combined measurement of IL-18 and TGF-β1 in plasma can be considered as a possible biomarker of psoriasis activity [<xref ref-type="bibr" rid="R9">9</xref>]. Kato <italic>et al.</italic> indicated that a single nucleotide polymorphism (SNP) in the promoter of the IL-18 gene is associated with the presence of psoriasis, but not atopic dermatitis. This suggests that the SNP is associated with susceptibility to psoriasis vulgaris, presumably by affecting the production of IL-18 [<xref ref-type="bibr" rid="R10">10</xref>].</p><p>A few studies have analysed IL-18 expression in psoriasis vulgaris [<xref ref-type="bibr" rid="R11">11</xref>-<xref ref-type="bibr" rid="R14">14</xref>]. Much less information is available on the ability of different therapeutic interventions to modulate ongoing changes in expression in chronic disorders, especially in a human disease such as psoriasis.</p><p>The aim of this study was to demonstrate expression of IL-18 in keratinocytes from psoriatic lesions in comparison to keratinocytes from non-lesional skin and to study the change of IL-18 expression after therapeutic interventions.</p></sec><sec sec-type="materials|methods"><title>Material and methods</title><p>This study included 16 patients. The patients were selected from the outpatient clinic of Benha University hospital, Al-Haud Al-Marsoud and The National Research Center, Cairo, Egypt. The patients included 4 women (25%) and 12 men (75%), and their ages ranged from 21 to 62 years with a mean of 40.87 ±13.17 years. They had different clinical subtypes of psoriasis and exhibited various degrees of severity. History of present illness included the onset, course, duration, previous treatment and date of last topical application or systemic therapy. Inclusion criteria: those included in the study were adult psoriatic patients ≥ 18 years old who should not have received topical therapeutic modality apart from petrolatum for the last 4 weeks or systemic therapy for the last 8 weeks.</p><p>Presence or absence of any coexisting non-cutaneous conditions was established. Three patients were hypertensive, 3 had psoriatic arthropathy, 1 had hepatitis C virus (HCV) with anaemia of chronic disorder type and 1 had diabetes mellitus with hypertension. A positive family history was encountered only in one case. Two patients had upper respiratory tract infections; cold exposure worsened 5 patients' clinical presentation; sweating aggravated 1 patient's condition; 3 patients had psychogenic factors precipitating their cases. Only 5 of the studied patients were not affected by predisposing factors (31.3%).</p><p>The psoriasis area and severity index (PASI) score sheet was adopted according to Fredriksson and Pettersson and Marks <italic>et al.</italic> [<xref ref-type="bibr" rid="R15">15</xref>, <xref ref-type="bibr" rid="R16">16</xref>] for all patients as an indication of degree of severity. In mild cases, the extent of the disease does not exceed 10, while in severe cases, the extent of the disease is more than 30 based on the PASI score [<xref ref-type="bibr" rid="R17">17</xref>]. Informed consent was obtained after complete description of the study from each participant, according to the guidelines of the local ethical committee of the National Research Center.</p><p>Two biopsies were taken from each patient in the first set. The first biopsy was taken from the lesional psoriatic skin and the second from non-lesional skin. All patients were subjected to continuous treatment (in the form of topical steroids and oral systemic methotrexate) and follow-up for 2 months. Six patients with moderate to severe degrees of psoriasis and 2 with palmoplantar subtype of psoriasis (who did not respond to topical steroids) received oral systemic methotrexate. The dosage used was 15 mg per week in three divided doses taken at 12-h intervals during a 24-h period with folate supplementation. Patients receiving systemic methotrexate were subjected to monitoring liver blood tests monthly. Eight patients with mild to moderate degrees of psoriasis were maintained on topical steroid creams and ointments in the form of betamethasone dipropionate, twice daily. At the end of the 2-month course, a third psoriatic lesional skin biopsy was taken from all patients.</p><sec><title>Tissue processing and preparation</title><p>To prevent degradation by intracellular RNases, tissues were embedded soon after excision in the RNA stabilizing reagent RNAlater provided by QIAGEN worldwide companies according to the manufacturer's instructions to be stored at –70°C before sample processing. Frozen tissues were not allowed to thaw during handling to be ready for quantitative PCR determination of IL-18. Each biopsy was weighed before processing. Biopsies weighed up to 20 mg. Efficient disruption and homogenization of the starting material was performed using a rotor-stator homogenizer.</p></sec><sec><title>RNA extraction and complementary (c)DNA synthesis</title><p>Total RNA was extracted using QIA amp RNA extraction kit provided by QIAGEN worldwide companies. The concentration and purity of RNA was determined by measuring its absorbance at 260 nm (A<sub>260</sub>) and 280 nm (A<sub>280</sub>) in a UV visible spectrophotometer. Total RNA (2 µg) was reverse-transcribed to cDNA with High-Capacity cDNA Archive kit provided by Applied Biosystems. cDNAs were stored at –20°C until real-time quantitative PCR was performed.</p></sec><sec><title>Gene expression quantitation</title><p>Real-time quantitative PCR was performed using the Applied Biosystems Perkin Elmer 7300 sequence detection system. Primers were IL-18 sense primer 5'-AGG AAT AAA GAT GGC TGC TGA AC-3' and anti-sense primer 5'-GCT CAC CAC AAC CTC TAC CTC C-3'. Each PCR reaction (in a 50 µl volume) contained 1× of TaqMan universal PCR master mix, (2×) 50 nM to 900 nM of forward primer, 50 nM to 900 nM of reverse primer, 250 nM of TaqMan probe labelled with 6-carboxyfluorescein (FAM) and 10 ng to 100 ng of cDNA. The amplification protocol was 2 min at 50°C, 10 min at 95°C, then 40 cycles for 15 s at 95°C and 1 min at 60°C. Relative gene expression was determined for each sample. Amplification of the gene for glyceraldehydes-3-phosphate dehydrogenase (GAPDH), a constitutively expressed housekeeping gene, was performed on all samples. All genes were subsequently normalized against GAPDH levels. In this study mRNA levels were expressed in picograms/microlitre (pg/µl).</p></sec><sec><title>Statistical analysis</title><p>All data were collected from the patient charts and entered into a computerized spreadsheet. The fit of the data to the normal distribution was tested with Kolmogorov-Smirnov test. Since the distribution of the data was significantly different from normal, nonparametric statistics were further used. Comparisons were made using Mann-Whitney <italic>U</italic>, Kruskal Wallis and Wilcoxon signed ranks tests between variables adjusted for appropriate covariates, and Spearman rank correlation coefficients were calculated between the assessed variables. The null hypothesis was rejected with a two sided <italic>p</italic> value of < 0.05. All analyses were performed with SPSS 11.0 for Macintosh (Statistical Package for the Social Sciences. SPSS Inc., Chicago, IL, USA).</p></sec></sec><sec><title>Results</title><p>Demographic and behavioural characteristics of included patients are presented in Table <xref ref-type="table" rid="T1">I</xref>. PASI score used to evaluate the severity of psoriasis varied from 1.4 to 39 with a mean of 14.96 ±11.82. According to Ramsay and Lawrence [<xref ref-type="bibr" rid="R17">17</xref>], the PASI score grades in the different subtypes of psoriasis are demonstrated in Table <xref ref-type="table" rid="T2">II</xref>.</p><sec><title>Results of quantitative PCR</title><p>All patients had detectable levels of IL-18 mRNA in the uninvolved skin. Table <xref ref-type="table" rid="T3">III</xref> shows the range, mean ± SD and median levels of cytokine gene expression in each skin biopsy at the different times before and after receiving medication, whether topical or systemic. Comparisons between median IL-18 expression levels with percentiles in all studied patients are demonstrated in Figure <xref ref-type="fig" rid="F1">1</xref>.</p><p>Significantly lower IL-18 expression in uninvolved skin than lesional skin before treatment (<italic>p</italic> = 0.001) and lesional skin after treatment (<italic>p</italic> = 0.002) was demonstrated in all studied patients as one group. The IL-18 expression in the psoriatic skin lesions after treatment was significantly lower than lesional skin before treatment (<italic>p</italic> = 0.023).</p><p>Interleukin-18 mean expression levels in non-lesional and lesional skin (before and after treatment) in the different subtypes of psoriasis are shown in Figure <xref ref-type="fig" rid="F2">2</xref>. The highest expression level was noted in plaque and nails clinical subtype of psoriasis.</p><p>Among patients receiving topical steroids, significantly lower IL-18 expression was detected in non-lesional skin than lesional skin before treatment (<italic>p</italic> = 0.012) and after treatment (<italic>p</italic> = 0.012). No statistical significance could be obtained when comparing the change of expression before and after topical treatment (<italic>p</italic> = 0.069).</p><p>Individual variations of the IL-18 expression levels in non-lesional and lesional skin before and after receiving topical steroids are demonstrated in Figure <xref ref-type="fig" rid="F3">3</xref>.</p><p>In patients receiving methotrexate, significantly lower IL-18 expression was detected in non-lesional skin than lesional skin before treatment (<italic>p</italic> = 0.018). The change of expression levels in lesional skin after treatment was not statistically significant when compared to non-lesional skin (<italic>p</italic> = 0.069) and lesional skin before treatment (<italic>p</italic> = 0.12).</p><p>Individual variations of the IL-18 expression levels in non-lesional and lesional skin before and after receiving systemic methotrexate are demonstrated in Figure <xref ref-type="fig" rid="F4">4</xref>.</p><p>In the correlation between IL-18 expression and different demographic and behavioural characteristics of the studied patients as one group. A significant correlation was obtained between IL-18 expression levels in psoriatic lesions and the severity of psoriasis expressed by PASI score (<italic>r</italic> = 0.72 and <italic>p</italic> = 0.001) and disease duration (<italic>r</italic> = 0.40 and <italic>p</italic> = 0.01). No significant correlation could be revealed between IL-18 expression levels in psoriatic lesions and patients’ age, smoking state, predisposing factors or coexisting diseases.</p></sec></sec><sec><title>Discussion</title><p>In the present study our patients with psoriasis showed significantly increased IL-18 expression in keratinocytes from psoriatic lesions before and after receiving medication, whether topical or systemic, in comparison to keratinocytes from non-lesional skin. This supports the concept of psoriasis as a T cell mediated autoimmune disease. Krueger [<xref ref-type="bibr" rid="R18">18</xref>] reported, in his study on a large series of skin biopsies, increased protein expression in lesional as opposed to non-lesional skin samples. Other investigators stated that lesional skin might be the source of the elevated plasma levels of IL-18 [<xref ref-type="bibr" rid="R19">19</xref>]. In the present work quantitative PCR using mRNA taken from uninvolved skin revealed low but detectable levels of IL-18 mRNA expression. This finding is consistent with previous studies showing that human keratinocytes are capable of synthesizing low levels of IL-18 mRNA even under non-stimulated conditions [<xref ref-type="bibr" rid="R20">20</xref>]. Chang <italic>et al.</italic> [<xref ref-type="bibr" rid="R21">21</xref>] found that genes specifically modulated in uninvolved skin play a major role in triggering disease progression, and in being the first candidate for early disease diagnosis or development of new therapies. This finding could allow selection of the treatment regimen for individual patients.</p><p>In the present study the IL-18 expression in patients receiving topical steroids was significantly lower in non-lesional skin than lesional skin before and after treatment. After 2 months of treatment, IL-18 expression levels returned back to low values but not significantly lower than the lesional skin levels of expression before treatment. In patients receiving methotrexate non-lesional skin expression was significantly lower than lesional skin before treatment. Also the expression levels decreased after treatment but still not statistically significantly when compared to lesional skin before treatment. Otkjaer <italic>et al.</italic> [<xref ref-type="bibr" rid="R22">22</xref>] determined IL-19 and IL-20 in non-lesional and lesional psoriatic skin after 28 days of treatment using topical calcipotriol ointment for mild cases and oral cyclosporine for moderate and severe cases. They found that in patients treated with calcipotriol, IL-19 and IL-20 mRNA expression levels in lesional psoriatic skin were reduced after 14 days, but the reduction became statistically significant only after 28 days. So the timing for mRNA expression analysis could affect the degree of significance. This is further supported by studies demonstrating a decrease of IL-18 levels after narrowband UVB therapy together with other parameters characteristic of the T helper 1 (Th1) response [<xref ref-type="bibr" rid="R23">23</xref>]. Apart from stimulating the Th1 response, IL-18 can regulate the Th2 response depending on the local cytokine network. Interleukin-12 enhances IFN-γ production induced by IL-18, whereas IL-18 alone induces IL- 4 and IL-13 production [<xref ref-type="bibr" rid="R24">24</xref>].</p><p>Classic systemic treatments for psoriasis have not fully met the needs of patients for permanent improvement. Antibody-based or fusion protein-based selective targeting of key mediators of inflammation has been added to the treatment approaches for psoriasis. Kong <italic>et al.</italic> and Smeltz [<xref ref-type="bibr" rid="R25">25</xref>, <xref ref-type="bibr" rid="R26">26</xref>] studied IL-18 receptor blocking by using 1,25-dihydroxyvitamin D in mouse keratinocytes. They observed that the IL-18 expression level was decreased synchronously with treatment. Tak <italic>et al.</italic> [<xref ref-type="bibr" rid="R27">27</xref>] investigated IL-18 binding protein (IL-18 BP) in psoriatic patients and demonstrated that IL-18 BP neutralizes the activity of IL-18.</p><p>Our study demonstrated that IL-18 expression in the psoriatic lesions before treatment was significantly correlated with the severity of psoriasis and disease duration. No correlation was noted between IL-18 expression and patients’ age, smoking status, predisposing factors or coexisting diseases. Flisiak <italic>et al.</italic> [<xref ref-type="bibr" rid="R9">9</xref>] found a significant correlation between plasma IL-18 levels and PASI values. But, they did not find any correlation between IL-18 concentration in scales and PASI score. Trinquet <italic>et al.</italic> [<xref ref-type="bibr" rid="R28">28</xref>] studied the influence of disease duration on gene expression profiles in psoriasis. They did not reveal any correlation. However, Craven <italic>et al.</italic> [<xref ref-type="bibr" rid="R29">29</xref>] studied IL-10 in early and late onset psoriasis and confirmed that IL-10 expression was correlated significantly with disease duration. Sampogna <italic>et al.</italic> [<xref ref-type="bibr" rid="R30">30</xref>] studied 380 patients with psoriasis and did not find any significant difference between older and younger patients regarding IL-18 levels. On the other hand, Chen <italic>et al.</italic> [<xref ref-type="bibr" rid="R31">31</xref>] found a statistically significant correlation between IL-18 expression levels and age with <italic>p</italic> value equal to 0.015.</p><p>In conclusion, based on the increased IL-18 expression levels in psoriatic skin lesions relative to uninvolved skin, this cytokine appears to be crucial in the development of the active psoriatic lesion itself, where it is produced locally by a step in the evolution of the psoriatic lesion. This could be supported by its correlation with clinical severity, disease duration and its expression reduction after treatment. The decreased expression after treatment was not to an extent indicating that the skin had returned to normal. The small size of the study population and the heterogeneous group of patients participating in the study preclude the drawing of firm conclusions. Future studies on genes involved and array based technologies should allow confirmation of the results. Also further studies are recommended to try other lines of treatment for psoriasis. 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person-group-type="author"><name><surname>Trinquet</surname><given-names>QV</given-names></name><name><surname>Fogel</surname><given-names>P</given-names></name><name><surname>Aldana-Jammayrac</surname><given-names>O</given-names></name><etal></etal></person-group><article-title>Interleukin 19 gene expression profile in psoriasis: anaylsis of impact of body site location and clinical severity</article-title><source>Br J Dermatol</source><year>2005</year><volume>152</volume><fpage>489</fpage><lpage>504</lpage><pub-id pub-id-type="pmid">15787818</pub-id></element-citation></ref><ref id="R29"><label>29</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Craven</surname><given-names>NM</given-names></name><name><surname>Jackson</surname><given-names>CW</given-names></name><name><surname>Kirby</surname><given-names>B</given-names></name><etal></etal></person-group><article-title>Cytokine gene polymorphism in psoriasis</article-title><source>Br J Dermatol</source><year>2001</year><volume>144</volume><fpage>849</fpage><lpage>53</lpage><pub-id pub-id-type="pmid">11298547</pub-id></element-citation></ref><ref id="R30"><label>30</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sampogna</surname><given-names>F</given-names></name><name><surname>Tabolli</surname><given-names>S</given-names></name><name><surname>Söderfeldt</surname><given-names>B</given-names></name><etal></etal></person-group><article-title>Measuring quality of life of patients with different clinical types of psoriasis using the SF-36</article-title><source>Br J Dermatol</source><year>2006</year><volume>154</volume><fpage>844</fpage><lpage>9</lpage><pub-id pub-id-type="pmid">16634884</pub-id></element-citation></ref><ref id="R31"><label>31</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>WJ</given-names></name><name><surname>Yang</surname><given-names>JY</given-names></name><name><surname>Lin</surname><given-names>JH</given-names></name></person-group><article-title>Nasopharyngeal shedding of severe acute respiratory syndrome associated corona virus is associated with genetic polymorphism</article-title><source>Clin Infect Dis</source><year>2006</year><volume>42</volume><fpage>1561</fpage><lpage>9</lpage><pub-id pub-id-type="pmid">16652313</pub-id></element-citation></ref></ref-list></back><floats-group><fig id="F1" position="float"><label>Figure 1</label><caption><p>The comparisons between median IL-18 expression levels with percentiles in all studied
patients</p><p>1 – IL-18 in lesional skin before treatment, 2 – IL-18 in non lesional skin, 3 – IL-18 in lesional skin after treatment</p></caption><graphic xlink:href="AMS-7-4-713_F1"></graphic></fig><fig id="F2" position="float"><label>Figure 2</label><caption><p>Interleukin-18 mean expression levels in non-lesional and lesional skin (before and after treatment) in the different subtypes of psoriasis</p></caption><graphic xlink:href="AMS-7-4-713_F2"></graphic></fig><fig id="F3" position="float"><label>Figure 3</label><caption><p>Individual variations of the IL-18 expression levels in non-lesional and lesional skin before and after re ceiv ing topical steroids</p></caption><graphic xlink:href="AMS-7-4-713_F3"></graphic></fig><fig id="F4" position="float"><label>Figure 4</label><caption><p>Individual variations of the IL-18 expression levels in non-lesional and lesional skin before and after receiving systemic methotrexate</p></caption><graphic xlink:href="AMS-7-4-713_F4"></graphic></fig><table-wrap id="T1" position="float"><label>Table I</label><caption><p>Demographic and behavioural characteristics of patients with psoriasis</p></caption><table frame="border" rules="rows" width="100%"><thead><tr><th align="left" rowspan="1" colspan="1">Characteristics</th><th align="center" rowspan="1" colspan="1">Patients</th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1">Age range (mean ± SD) [years]</td><td align="center" rowspan="1" colspan="1">21-62 (40.875 ±13.17)</td></tr><tr><td align="left" rowspan="1" colspan="1">Sex (M/F)</td><td align="center" rowspan="1" colspan="1">12/4</td></tr><tr><td align="left" rowspan="1" colspan="1">Marital status (+/–)</td><td align="center" rowspan="1" colspan="1">13/3</td></tr><tr><td align="left" rowspan="1" colspan="1">Occupation (working/not working)</td><td align="center" rowspan="1" colspan="1">12/4</td></tr><tr><td align="left" rowspan="1" colspan="1">Smoking (+/–)</td><td align="center" rowspan="1" colspan="1">4/12</td></tr><tr><td align="left" rowspan="1" colspan="1">Duration of disease range (mean ± SD) [years]</td><td align="center" rowspan="1" colspan="1">2 months – 14 years
5.88 ±4.50</td></tr><tr><td align="left" rowspan="1" colspan="1">Family history (+/–)</td><td align="center" rowspan="1" colspan="1">1/15</td></tr><tr><td align="left" rowspan="1" colspan="1">Provoking factors (+/–)</td><td align="center" rowspan="1" colspan="1">11/5</td></tr><tr><td align="left" rowspan="1" colspan="1">Associated diseases (+/–)</td><td align="center" rowspan="1" colspan="1">8/8</td></tr></tbody></table></table-wrap><table-wrap id="T2" position="float"><label>Table II</label><caption><p>The range of the PASI score in the different types of psoriasis</p></caption><table frame="border" rules="rows" width="100%"><thead><tr><th rowspan="2" align="left" colspan="1">Type of psoriasis</th><th align="center" colspan="4" rowspan="1">PASI score grades</th></tr><tr><th align="center" rowspan="1" colspan="1">Mild<xref ref-type="table-fn" rid="T2FN2">*</xref></th><th align="center" rowspan="1" colspan="1">Moderate<xref ref-type="table-fn" rid="T2FN2">*</xref></th><th align="center" rowspan="1" colspan="1">Severe<xref ref-type="table-fn" rid="T2FN2">*</xref></th><th align="center" rowspan="1" colspan="1">Total (%)</th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1">Plaque-type</td><td align="center" rowspan="1" colspan="1">3</td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">1</td><td align="center" rowspan="1" colspan="1">6 (37.5)</td></tr><tr><td align="left" rowspan="1" colspan="1">Erythrodermic</td><td rowspan="1" colspan="1"></td><td rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">1</td><td align="center" rowspan="1" colspan="1">1 (6.3)</td></tr><tr><td align="left" rowspan="1" colspan="1">Guttate</td><td rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">1</td><td rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">1 (6.3)</td></tr><tr><td align="left" rowspan="1" colspan="1">Flexural</td><td align="center" rowspan="1" colspan="1">1</td><td rowspan="1" colspan="1"></td><td rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">1 (6.3)</td></tr><tr><td align="left" rowspan="1" colspan="1">Palmoplantar</td><td align="center" rowspan="1" colspan="1">2</td><td rowspan="1" colspan="1"></td><td rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">2 (12.5)</td></tr><tr><td align="left" rowspan="1" colspan="1">Plaque and gutt</td><td rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">2</td><td rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">2 (12.5)</td></tr><tr><td align="left" rowspan="1" colspan="1">Plaque and p.p</td><td align="center" rowspan="1" colspan="1">1</td><td rowspan="1" colspan="1"></td><td rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">1 (6.3)</td></tr><tr><td align="left" rowspan="1" colspan="1">Plaque and flex</td><td rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">1</td><td rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">1 (6.3)</td></tr><tr><td align="left" rowspan="1" colspan="1">Plaque and nails</td><td rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">1</td><td rowspan="1" colspan="1"></td><td align="center" rowspan="1" colspan="1">1 (6.3)</td></tr><tr><td align="left" rowspan="1" colspan="1">Total</td><td align="center" rowspan="1" colspan="1">7</td><td align="center" rowspan="1" colspan="1">7</td><td align="center" rowspan="1" colspan="1">2</td><td align="center" rowspan="1" colspan="1">16</td></tr></tbody></table><table-wrap-foot><fn id="T2FN1"><p>gutt – guttate, p.p – palmoplantar, flex – flexural</p></fn><fn id="T2FN2"><label>*</label><p>According to Ramsay and Lawrence [<xref ref-type="bibr" rid="R17">17</xref>]: mild cases – the PASI score did not exceed 10, moderate cases – the PASI score was > 10 to 30, severe cases – the PASI score was more than 30</p></fn></table-wrap-foot></table-wrap><table-wrap id="T3" position="float"><label>Table III</label><caption><p>Interleukin-18 expression levels in psoriatic patients</p></caption><table frame="border" rules="rows" width="100%"><thead><tr><th align="left" rowspan="2" colspan="1">Variant</th><th align="center" rowspan="2" colspan="1"><italic>N</italic></th><th align="center" rowspan="2" colspan="1">Mean ± SD</th><th align="center" rowspan="2" colspan="1">Range</th><th align="center" colspan="3" rowspan="1">Percentiles</th></tr><tr><th align="center" rowspan="1" colspan="1">25th</th><th align="center" rowspan="1" colspan="1">50<sup>th</sup> (Median)</th><th align="center" rowspan="1" colspan="1">75<sup>th</sup></th></tr></thead><tbody><tr><td colspan="7" align="center" rowspan="1"><bold>All studied patients</bold></td></tr><tr><td align="left" rowspan="1" colspan="1">IL-18 NL</td><td align="center" rowspan="1" colspan="1">16</td><td align="center" rowspan="1" colspan="1">1.10 ±2.14</td><td align="center" rowspan="1" colspan="1">0.01-7.90</td><td align="center" rowspan="1" colspan="1">0.0300</td><td align="center" rowspan="1" colspan="1">0.1850<xref ref-type="table-fn" rid="T3FN2">a</xref></td><td align="center" rowspan="1" colspan="1">1.2675</td></tr><tr><td align="left" rowspan="1" colspan="1">IL-18 LB</td><td align="center" rowspan="1" colspan="1">16</td><td align="center" rowspan="1" colspan="1">3.24 ±5.63</td><td align="center" rowspan="1" colspan="1">0.17-22.70</td><td align="center" rowspan="1" colspan="1">0.4900</td><td align="center" rowspan="1" colspan="1">0.9050</td><td align="center" rowspan="1" colspan="1">4.9000</td></tr><tr><td align="left" rowspan="1" colspan="1">IL-18 LA</td><td align="center" rowspan="1" colspan="1">16</td><td align="center" rowspan="1" colspan="1">1.78 ±2.72</td><td align="center" rowspan="1" colspan="1">0.01-8.10</td><td align="center" rowspan="1" colspan="1">0.2750</td><td align="center" rowspan="1" colspan="1">0.4600<xref ref-type="table-fn" rid="T3FN3">b</xref></td><td align="center" rowspan="1" colspan="1">1.5250</td></tr><tr><td colspan="7" align="center" rowspan="1"><bold>Patients on TS</bold></td></tr><tr><td align="left" rowspan="1" colspan="1">IL-18 NL</td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">0.26 ±0.44</td><td align="center" rowspan="1" colspan="1">0.01-1.3</td><td align="center" rowspan="1" colspan="1">0.014</td><td align="center" rowspan="1" colspan="1">0.051<xref ref-type="table-fn" rid="T3FN4">c</xref></td><td align="center" rowspan="1" colspan="1">0.337</td></tr><tr><td align="left" rowspan="1" colspan="1">IL-18 LB</td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">1.59 ±2.1</td><td align="center" rowspan="1" colspan="1">0.17-6.4</td><td align="center" rowspan="1" colspan="1">0.2275</td><td align="center" rowspan="1" colspan="1">0.53</td><td align="center" rowspan="1" colspan="1">2.53</td></tr><tr><td align="left" rowspan="1" colspan="1">IL-18 LA</td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">0.61 ±0.58</td><td align="center" rowspan="1" colspan="1">0.012-1.6</td><td align="center" rowspan="1" colspan="1">0.1275</td><td align="center" rowspan="1" colspan="1">0.415</td><td align="center" rowspan="1" colspan="1">1.2</td></tr><tr><td colspan="7" align="center" rowspan="1"><bold>Patients on MTX</bold></td></tr><tr><td align="left" rowspan="1" colspan="1">IL-18 NL</td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">1.93 ±2.84</td><td align="center" rowspan="1" colspan="1">0.012-7.9</td><td align="center" rowspan="1" colspan="1">0.1625</td><td align="center" rowspan="1" colspan="1">0.685<xref ref-type="table-fn" rid="T3FN5">d</xref></td><td align="center" rowspan="1" colspan="1">3.775</td></tr><tr><td align="left" rowspan="1" colspan="1">IL-18 LB</td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">4.89 ±7.56</td><td align="center" rowspan="1" colspan="1">0.49-22.7</td><td align="center" rowspan="1" colspan="1">0.602</td><td align="center" rowspan="1" colspan="1">1.385</td><td align="center" rowspan="1" colspan="1">6.2</td></tr><tr><td align="left" rowspan="1" colspan="1">IL-18 LA</td><td align="center" rowspan="1" colspan="1">8</td><td align="center" rowspan="1" colspan="1">2.95 ±3.52</td><td align="center" rowspan="1" colspan="1">0.26-8.10</td><td align="center" rowspan="1" colspan="1">0.3275</td><td align="center" rowspan="1" colspan="1">0.745</td><td align="center" rowspan="1" colspan="1">7.26</td></tr></tbody></table><table-wrap-foot><fn id="T3FN1"><p>IL-18NL – IL-18 in non-lesional skin, IL-18LB – IL-18 in lesional skin before treatment, IL-18LA – IL-18 in lesional skin after treatment, TS – topical steroids, MTX – methotrexate</p></fn><fn id="T3FN2"><label>a</label><p>significantly lower than lesional skin before treatment (p = 0.001) and lesional skin after treatment (p = 0.002)</p></fn><fn id="T3FN3"><label>b</label><p>significantly lower than lesional skin before treatment (p = 0.023)</p></fn><fn id="T3FN4"><label>c</label><p>significantly lower than lesional skin before treatment (p = 0.012) and after treatment (p = 0.012)</p></fn><fn id="T3FN5"><label>d</label><p>significantly lower than lesional skin before treatment (p = 0.018)</p></fn></table-wrap-foot></table-wrap></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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