CovidV2, Pmc, Corpus, bibRecord, 000707

Development and Evaluation of Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid and Real-Time Detection of the Swine-Origin Influenza A H1N1 Virus

Identifieur interne : 000707 ( Pmc/Corpus ); précédent : 000706; suivant : 000708

Development and Evaluation of Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid and Real-Time Detection of the Swine-Origin Influenza A H1N1 Virus

Auteurs : Manmohan Parida ; Jyoti Shukla ; Shashi Sharma ; Sanna Ranghia Santhosh ; Vasanthapuram Ravi ; Reeta Mani ; Maria Thomas ; Shashi Khare ; Arvind Rai ; Radha Kant Ratho ; Sujit Pujari ; Bijayanti Mishra ; Putcha Venkata Lakshmana Rao ; Rajagopalan Vijayaraghavan

Source :

The Journal of Molecular Diagnostics : JMD [ 1525-1578 ] ; 2011.

RBID : PMC:3069812

Abstract

The recent emergence of the swine-origin influenza A H1N1 virus (S-OIV) poses a serious global health threat. Rapid detection and differentiation of S-OIV from seasonal influenza is crucial for patient management and control of the epidemics. A one-step, single-tube accelerated and quantitative S-OIV-specific H1 reverse transcription loop-mediated isothermal amplification (RTLAMP) assay for clinical diagnosis of S-OIV by targeting the H1 gene is reported in this article. A comparative evaluation of the H1-specific RTLAMP assay vis-à-vis the World Health Organization-approved real-time polymerase chain reaction (RTPCR), involving 239 acute-phase throat swab samples, demonstrated exceptionally higher sensitivity by picking up all of the 116 H1N1-positive cases and 36 additional positive cases among the negatives that were sequence-confirmed as S-OIV H1N1. None of the real-time RTPCR-positive samples were missed by the RTLAMP system. The comparative analysis revealed that S-OIV RTLAMP was up to tenfold more sensitive than the World Health Organization real-time RTPCR; it had a detection limit of 0.1 tissue culture infectious dosage of ₅₀/ml. One of the most attractive features of this isothermal gene amplification assay is that it seems to have an advantage in monitoring gene amplification by means of SYBR Green I dye-mediated naked-eye visualization within 30 minutes compared to 2 to 3 hours for a real-time reverse transcription polymerase chain reaction. This suggests that the RTLAMP assay is a valuable tool for rapid, real-time detection and quantification of S-OIV in acute-phase throat swab samples without requiring sophisticated equipment.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3069812

DOI: 10.1016/j.jmoldx.2010.11.003
PubMed: 21227400
PubMed Central: 3069812

Links to Exploration step

PMC:3069812

Le document en format XML

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<author><name sortKey="Parida, Manmohan" sort="Parida, Manmohan" uniqKey="Parida M" first="Manmohan" last="Parida">Manmohan Parida</name>
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</affiliation>
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<author><name sortKey="Shukla, Jyoti" sort="Shukla, Jyoti" uniqKey="Shukla J" first="Jyoti" last="Shukla">Jyoti Shukla</name>
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<author><name sortKey="Sharma, Shashi" sort="Sharma, Shashi" uniqKey="Sharma S" first="Shashi" last="Sharma">Shashi Sharma</name>
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<author><name sortKey="Ranghia Santhosh, Sanna" sort="Ranghia Santhosh, Sanna" uniqKey="Ranghia Santhosh S" first="Sanna" last="Ranghia Santhosh">Sanna Ranghia Santhosh</name>
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</affiliation>
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<author><name sortKey="Ravi, Vasanthapuram" sort="Ravi, Vasanthapuram" uniqKey="Ravi V" first="Vasanthapuram" last="Ravi">Vasanthapuram Ravi</name>
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</affiliation>
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<author><name sortKey="Mani, Reeta" sort="Mani, Reeta" uniqKey="Mani R" first="Reeta" last="Mani">Reeta Mani</name>
<affiliation><nlm:aff id="aff2">Department of Virology, National Institute of Mental Health and Neuro Sciences, Bangalore, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Thomas, Maria" sort="Thomas, Maria" uniqKey="Thomas M" first="Maria" last="Thomas">Maria Thomas</name>
<affiliation><nlm:aff id="aff2">Department of Virology, National Institute of Mental Health and Neuro Sciences, Bangalore, India</nlm:aff>
</affiliation>
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<author><name sortKey="Khare, Shashi" sort="Khare, Shashi" uniqKey="Khare S" first="Shashi" last="Khare">Shashi Khare</name>
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</affiliation>
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<author><name sortKey="Rai, Arvind" sort="Rai, Arvind" uniqKey="Rai A" first="Arvind" last="Rai">Arvind Rai</name>
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</affiliation>
</author>
<author><name sortKey="Kant Ratho, Radha" sort="Kant Ratho, Radha" uniqKey="Kant Ratho R" first="Radha" last="Kant Ratho">Radha Kant Ratho</name>
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</affiliation>
</author>
<author><name sortKey="Pujari, Sujit" sort="Pujari, Sujit" uniqKey="Pujari S" first="Sujit" last="Pujari">Sujit Pujari</name>
<affiliation><nlm:aff id="aff4">Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mishra, Bijayanti" sort="Mishra, Bijayanti" uniqKey="Mishra B" first="Bijayanti" last="Mishra">Bijayanti Mishra</name>
<affiliation><nlm:aff id="aff4">Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lakshmana Rao, Putcha Venkata" sort="Lakshmana Rao, Putcha Venkata" uniqKey="Lakshmana Rao P" first="Putcha Venkata" last="Lakshmana Rao">Putcha Venkata Lakshmana Rao</name>
<affiliation><nlm:aff id="aff1">Division of Virology, Defence Research & Development Establishment, Gwalior, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Vijayaraghavan, Rajagopalan" sort="Vijayaraghavan, Rajagopalan" uniqKey="Vijayaraghavan R" first="Rajagopalan" last="Vijayaraghavan">Rajagopalan Vijayaraghavan</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development and Evaluation of Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid and Real-Time Detection of the Swine-Origin Influenza A H1N1 Virus</title>
<author><name sortKey="Parida, Manmohan" sort="Parida, Manmohan" uniqKey="Parida M" first="Manmohan" last="Parida">Manmohan Parida</name>
<affiliation><nlm:aff id="aff1">Division of Virology, Defence Research & Development Establishment, Gwalior, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Shukla, Jyoti" sort="Shukla, Jyoti" uniqKey="Shukla J" first="Jyoti" last="Shukla">Jyoti Shukla</name>
<affiliation><nlm:aff id="aff1">Division of Virology, Defence Research & Development Establishment, Gwalior, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sharma, Shashi" sort="Sharma, Shashi" uniqKey="Sharma S" first="Shashi" last="Sharma">Shashi Sharma</name>
<affiliation><nlm:aff id="aff1">Division of Virology, Defence Research & Development Establishment, Gwalior, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ranghia Santhosh, Sanna" sort="Ranghia Santhosh, Sanna" uniqKey="Ranghia Santhosh S" first="Sanna" last="Ranghia Santhosh">Sanna Ranghia Santhosh</name>
<affiliation><nlm:aff id="aff1">Division of Virology, Defence Research & Development Establishment, Gwalior, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ravi, Vasanthapuram" sort="Ravi, Vasanthapuram" uniqKey="Ravi V" first="Vasanthapuram" last="Ravi">Vasanthapuram Ravi</name>
<affiliation><nlm:aff id="aff2">Department of Virology, National Institute of Mental Health and Neuro Sciences, Bangalore, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mani, Reeta" sort="Mani, Reeta" uniqKey="Mani R" first="Reeta" last="Mani">Reeta Mani</name>
<affiliation><nlm:aff id="aff2">Department of Virology, National Institute of Mental Health and Neuro Sciences, Bangalore, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Thomas, Maria" sort="Thomas, Maria" uniqKey="Thomas M" first="Maria" last="Thomas">Maria Thomas</name>
<affiliation><nlm:aff id="aff2">Department of Virology, National Institute of Mental Health and Neuro Sciences, Bangalore, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Khare, Shashi" sort="Khare, Shashi" uniqKey="Khare S" first="Shashi" last="Khare">Shashi Khare</name>
<affiliation><nlm:aff id="aff3">Department of Virology, National Centre for Disease Control, Delhi, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Rai, Arvind" sort="Rai, Arvind" uniqKey="Rai A" first="Arvind" last="Rai">Arvind Rai</name>
<affiliation><nlm:aff id="aff3">Department of Virology, National Centre for Disease Control, Delhi, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kant Ratho, Radha" sort="Kant Ratho, Radha" uniqKey="Kant Ratho R" first="Radha" last="Kant Ratho">Radha Kant Ratho</name>
<affiliation><nlm:aff id="aff4">Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Pujari, Sujit" sort="Pujari, Sujit" uniqKey="Pujari S" first="Sujit" last="Pujari">Sujit Pujari</name>
<affiliation><nlm:aff id="aff4">Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mishra, Bijayanti" sort="Mishra, Bijayanti" uniqKey="Mishra B" first="Bijayanti" last="Mishra">Bijayanti Mishra</name>
<affiliation><nlm:aff id="aff4">Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lakshmana Rao, Putcha Venkata" sort="Lakshmana Rao, Putcha Venkata" uniqKey="Lakshmana Rao P" first="Putcha Venkata" last="Lakshmana Rao">Putcha Venkata Lakshmana Rao</name>
<affiliation><nlm:aff id="aff1">Division of Virology, Defence Research & Development Establishment, Gwalior, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Vijayaraghavan, Rajagopalan" sort="Vijayaraghavan, Rajagopalan" uniqKey="Vijayaraghavan R" first="Rajagopalan" last="Vijayaraghavan">Rajagopalan Vijayaraghavan</name>
<affiliation><nlm:aff id="aff1">Division of Virology, Defence Research & Development Establishment, Gwalior, India</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">The Journal of Molecular Diagnostics : JMD</title>
<idno type="ISSN">1525-1578</idno>
<idno type="eISSN">1943-7811</idno>
<imprint><date when="2011">2011</date>
</imprint>
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<front><div type="abstract" xml:lang="en"><p>The recent emergence of the swine-origin influenza A H1N1 virus (S-OIV) poses a serious global health threat. Rapid detection and differentiation of S-OIV from seasonal influenza is crucial for patient management and control of the epidemics. A one-step, single-tube accelerated and quantitative S-OIV-specific H1 reverse transcription loop-mediated isothermal amplification (RTLAMP) assay for clinical diagnosis of S-OIV by targeting the H1 gene is reported in this article. A comparative evaluation of the H1-specific RTLAMP assay vis-à-vis the World Health Organization-approved real-time polymerase chain reaction (RTPCR), involving 239 acute-phase throat swab samples, demonstrated exceptionally higher sensitivity by picking up all of the 116 H1N1-positive cases and 36 additional positive cases among the negatives that were sequence-confirmed as S-OIV H1N1. None of the real-time RTPCR-positive samples were missed by the RTLAMP system. The comparative analysis revealed that S-OIV RTLAMP was up to tenfold more sensitive than the World Health Organization real-time RTPCR; it had a detection limit of 0.1 tissue culture infectious dosage of <sub>50</sub>
/ml. One of the most attractive features of this isothermal gene amplification assay is that it seems to have an advantage in monitoring gene amplification by means of SYBR Green I dye-mediated naked-eye visualization within 30 minutes compared to 2 to 3 hours for a real-time reverse transcription polymerase chain reaction. This suggests that the RTLAMP assay is a valuable tool for rapid, real-time detection and quantification of S-OIV in acute-phase throat swab samples without requiring sophisticated equipment.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">J Mol Diagn</journal-id>
<journal-title-group><journal-title>The Journal of Molecular Diagnostics : JMD</journal-title>
</journal-title-group>
<issn pub-type="ppub">1525-1578</issn>
<issn pub-type="epub">1943-7811</issn>
<publisher><publisher-name>American Society for Investigative Pathology</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">21227400</article-id>
<article-id pub-id-type="pmc">3069812</article-id>
<article-id pub-id-type="publisher-id">JMDI16</article-id>
<article-id pub-id-type="doi">10.1016/j.jmoldx.2010.11.003</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Regular Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Development and Evaluation of Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid and Real-Time Detection of the Swine-Origin Influenza A H1N1 Virus</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Parida</surname>
<given-names>Manmohan</given-names>
</name>
<email>paridamm@rediffmail.com</email>
<xref rid="aff1" ref-type="aff">⁎</xref>
<xref rid="cor1" ref-type="corresp">⁎</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Shukla</surname>
<given-names>Jyoti</given-names>
</name>
<xref rid="aff1" ref-type="aff">⁎</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sharma</surname>
<given-names>Shashi</given-names>
</name>
<xref rid="aff1" ref-type="aff">⁎</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ranghia Santhosh</surname>
<given-names>Sanna</given-names>
</name>
<xref rid="aff1" ref-type="aff">⁎</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ravi</surname>
<given-names>Vasanthapuram</given-names>
</name>
<xref rid="aff2" ref-type="aff">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mani</surname>
<given-names>Reeta</given-names>
</name>
<xref rid="aff2" ref-type="aff">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Thomas</surname>
<given-names>Maria</given-names>
</name>
<xref rid="aff2" ref-type="aff">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Khare</surname>
<given-names>Shashi</given-names>
</name>
<xref rid="aff3" ref-type="aff">‡</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Rai</surname>
<given-names>Arvind</given-names>
</name>
<xref rid="aff3" ref-type="aff">‡</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kant Ratho</surname>
<given-names>Radha</given-names>
</name>
<xref rid="aff4" ref-type="aff">§</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Pujari</surname>
<given-names>Sujit</given-names>
</name>
<xref rid="aff4" ref-type="aff">§</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mishra</surname>
<given-names>Bijayanti</given-names>
</name>
<xref rid="aff4" ref-type="aff">§</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lakshmana Rao</surname>
<given-names>Putcha Venkata</given-names>
</name>
<xref rid="aff1" ref-type="aff">⁎</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Vijayaraghavan</surname>
<given-names>Rajagopalan</given-names>
</name>
<xref rid="aff1" ref-type="aff">⁎</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>⁎</label>
Division of Virology, Defence Research & Development Establishment, Gwalior, India</aff>
<aff id="aff2"><label>†</label>
Department of Virology, National Institute of Mental Health and Neuro Sciences, Bangalore, India</aff>
<aff id="aff3"><label>‡</label>
Department of Virology, National Centre for Disease Control, Delhi, India</aff>
<aff id="aff4"><label>§</label>
Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh, India</aff>
<author-notes><corresp id="cor1"><label>⁎</label>
Address reprint requests to Dr. Manmohan Parida, Division of Virology, Defence R & D Establishment, Jhansi Road, Gwalior 474002, M. P., India <email>paridamm@rediffmail.com</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>1</month>
<year>2011</year>
</pub-date>
<volume>13</volume>
<issue>1</issue>
<fpage>100</fpage>
<lpage>107</lpage>
<history><date date-type="accepted"><day>23</day>
<month>8</month>
<year>2010</year>
</date>
</history>
<permissions><copyright-statement>© 2011 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.</copyright-statement>
<copyright-year>2011</copyright-year>
<copyright-holder>American Society for Investigative Pathology and the Association for Molecular Pathology</copyright-holder>
</permissions>
<abstract><p>The recent emergence of the swine-origin influenza A H1N1 virus (S-OIV) poses a serious global health threat. Rapid detection and differentiation of S-OIV from seasonal influenza is crucial for patient management and control of the epidemics. A one-step, single-tube accelerated and quantitative S-OIV-specific H1 reverse transcription loop-mediated isothermal amplification (RTLAMP) assay for clinical diagnosis of S-OIV by targeting the H1 gene is reported in this article. A comparative evaluation of the H1-specific RTLAMP assay vis-à-vis the World Health Organization-approved real-time polymerase chain reaction (RTPCR), involving 239 acute-phase throat swab samples, demonstrated exceptionally higher sensitivity by picking up all of the 116 H1N1-positive cases and 36 additional positive cases among the negatives that were sequence-confirmed as S-OIV H1N1. None of the real-time RTPCR-positive samples were missed by the RTLAMP system. The comparative analysis revealed that S-OIV RTLAMP was up to tenfold more sensitive than the World Health Organization real-time RTPCR; it had a detection limit of 0.1 tissue culture infectious dosage of <sub>50</sub>
/ml. One of the most attractive features of this isothermal gene amplification assay is that it seems to have an advantage in monitoring gene amplification by means of SYBR Green I dye-mediated naked-eye visualization within 30 minutes compared to 2 to 3 hours for a real-time reverse transcription polymerase chain reaction. This suggests that the RTLAMP assay is a valuable tool for rapid, real-time detection and quantification of S-OIV in acute-phase throat swab samples without requiring sophisticated equipment.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>

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Development and Evaluation of Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid and Real-Time Detection of the Swine-Origin Influenza A H1N1 Virus

Development and Evaluation of Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid and Real-Time Detection of the Swine-Origin Influenza A H1N1 Virus

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