Comparison of Five Bacteriophages as Models for Viral Aerosol Studies
Identifieur interne : 000678 ( Pmc/Corpus ); précédent : 000677; suivant : 000679Comparison of Five Bacteriophages as Models for Viral Aerosol Studies
Auteurs : Nathalie Turgeon ; Marie-Josée Toulouse ; Bruno Martel ; Sylvain Moineau ; Caroline DuchaineSource :
- Applied and Environmental Microbiology [ 0099-2240 ] ; 2014.
Abstract
Bacteriophages are perceived to be good models for the study of airborne viruses because they are safe to use, some of them display structural features similar to those of human and animal viruses, and they are relatively easy to produce in large quantities. Yet, only a few studies have investigated them as models. It has previously been demonstrated that aerosolization, environmental conditions, and sampling conditions affect viral infectivity, but viral infectivity is virus dependent. Thus, several virus models are likely needed to study their general behavior in aerosols. The aim of this study was to compare the effects of aerosolization and sampling on the infectivity of five tail-less bacteriophages and two pathogenic viruses: MS2 (a single-stranded RNA [ssRNA] phage of the
Url:
DOI: 10.1128/AEM.00767-14
PubMed: 24795379
PubMed Central: 4068686
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PMC:4068686Le document en format XML
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<author><name sortKey="Turgeon, Nathalie" sort="Turgeon, Nathalie" uniqKey="Turgeon N" first="Nathalie" last="Turgeon">Nathalie Turgeon</name>
<affiliation><nlm:aff id="aff1">Institut Universitaire de Cardiologie et de Pneumologie de Québec, Hôpital Laval, Sainte-Foy, Quebec City, Quebec, Canada</nlm:aff>
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<affiliation><nlm:aff id="aff2">Département de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et Génie, Université Laval, Quebec City, Quebec, Canada</nlm:aff>
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<author><name sortKey="Toulouse, Marie Josee" sort="Toulouse, Marie Josee" uniqKey="Toulouse M" first="Marie-Josée" last="Toulouse">Marie-Josée Toulouse</name>
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<author><name sortKey="Martel, Bruno" sort="Martel, Bruno" uniqKey="Martel B" first="Bruno" last="Martel">Bruno Martel</name>
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<author><name sortKey="Moineau, Sylvain" sort="Moineau, Sylvain" uniqKey="Moineau S" first="Sylvain" last="Moineau">Sylvain Moineau</name>
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<affiliation><nlm:aff id="aff3">Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada</nlm:aff>
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<author><name sortKey="Duchaine, Caroline" sort="Duchaine, Caroline" uniqKey="Duchaine C" first="Caroline" last="Duchaine">Caroline Duchaine</name>
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<author><name sortKey="Turgeon, Nathalie" sort="Turgeon, Nathalie" uniqKey="Turgeon N" first="Nathalie" last="Turgeon">Nathalie Turgeon</name>
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<affiliation><nlm:aff id="aff2">Département de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et Génie, Université Laval, Quebec City, Quebec, Canada</nlm:aff>
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<author><name sortKey="Toulouse, Marie Josee" sort="Toulouse, Marie Josee" uniqKey="Toulouse M" first="Marie-Josée" last="Toulouse">Marie-Josée Toulouse</name>
<affiliation><nlm:aff id="aff1">Institut Universitaire de Cardiologie et de Pneumologie de Québec, Hôpital Laval, Sainte-Foy, Quebec City, Quebec, Canada</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff2">Département de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et Génie, Université Laval, Quebec City, Quebec, Canada</nlm:aff>
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<author><name sortKey="Martel, Bruno" sort="Martel, Bruno" uniqKey="Martel B" first="Bruno" last="Martel">Bruno Martel</name>
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</affiliation>
<affiliation><nlm:aff id="aff3">Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada</nlm:aff>
</affiliation>
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<author><name sortKey="Moineau, Sylvain" sort="Moineau, Sylvain" uniqKey="Moineau S" first="Sylvain" last="Moineau">Sylvain Moineau</name>
<affiliation><nlm:aff id="aff2">Département de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et Génie, Université Laval, Quebec City, Quebec, Canada</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff3">Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada</nlm:aff>
</affiliation>
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<author><name sortKey="Duchaine, Caroline" sort="Duchaine, Caroline" uniqKey="Duchaine C" first="Caroline" last="Duchaine">Caroline Duchaine</name>
<affiliation><nlm:aff id="aff1">Institut Universitaire de Cardiologie et de Pneumologie de Québec, Hôpital Laval, Sainte-Foy, Quebec City, Quebec, Canada</nlm:aff>
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<affiliation><nlm:aff id="aff2">Département de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et Génie, Université Laval, Quebec City, Quebec, Canada</nlm:aff>
</affiliation>
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<series><title level="j">Applied and Environmental Microbiology</title>
<idno type="ISSN">0099-2240</idno>
<idno type="eISSN">1098-5336</idno>
<imprint><date when="2014">2014</date>
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<front><div type="abstract" xml:lang="en"><p>Bacteriophages are perceived to be good models for the study of airborne viruses because they are safe to use, some of them display structural features similar to those of human and animal viruses, and they are relatively easy to produce in large quantities. Yet, only a few studies have investigated them as models. It has previously been demonstrated that aerosolization, environmental conditions, and sampling conditions affect viral infectivity, but viral infectivity is virus dependent. Thus, several virus models are likely needed to study their general behavior in aerosols. The aim of this study was to compare the effects of aerosolization and sampling on the infectivity of five tail-less bacteriophages and two pathogenic viruses: MS2 (a single-stranded RNA [ssRNA] phage of the <named-content content-type="genus-species">Leviviridae</named-content>
family), Φ6 (a segmented double-stranded RNA [dsRNA] phage of the <named-content content-type="genus-species">Cystoviridae</named-content>
family), ΦX174 (a single-stranded DNA [ssDNA] phage of the <named-content content-type="genus-species">Microviridae</named-content>
family), PM2 (a double-stranded DNA [dsDNA] phage of the <named-content content-type="genus-species">Corticoviridae</named-content>
family), PR772 (a dsDNA phage of the <named-content content-type="genus-species">Tectiviridae</named-content>
family), human influenza A virus H1N1 (an ssRNA virus of the <named-content content-type="genus-species">Orthomyxoviridae</named-content>
family), and the poultry virus Newcastle disease virus (NDV; an ssRNA virus of the <named-content content-type="genus-species">Paramyxoviridae</named-content>
family). Three nebulizers and two nebulization salt buffers (with or without organic fluid) were tested, as were two aerosol sampling devices, a liquid cyclone (SKC BioSampler) and a dry cyclone (National Institute for Occupational Safety and Health two-stage cyclone bioaerosol sampler). The presence of viruses in collected air samples was detected by culture and quantitative PCR (qPCR). Our results showed that these selected five phages behave differently when aerosolized and sampled. RNA phage MS2 and ssDNA phage ΦX174 were the most resistant to aerosolization and sampling. The presence of organic fluid in the nebulization buffer protected phages PR772 and Φ6 throughout the aerosolization and sampling with dry cyclones. In this experimental setup, the behavior of the influenza virus resembled that of phages PR772 and Φ6, while the behavior of NDV was closer to that of phages MS2 and ΦX174. These results provide critical information for the selection of appropriate phage models to mimic the behavior of specific human and animal viruses in aerosols.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Appl. Environ. Microbiol</journal-id>
<journal-id journal-id-type="hwp">aem</journal-id>
<journal-id journal-id-type="pmc">aem</journal-id>
<journal-id journal-id-type="publisher-id">AEM</journal-id>
<journal-title-group><journal-title>Applied and Environmental Microbiology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0099-2240</issn>
<issn pub-type="epub">1098-5336</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
<publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24795379</article-id>
<article-id pub-id-type="pmc">4068686</article-id>
<article-id pub-id-type="publisher-id">00767-14</article-id>
<article-id pub-id-type="doi">10.1128/AEM.00767-14</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Public and Environmental Health Microbiology</subject>
</subj-group>
</article-categories>
<title-group><article-title>Comparison of Five Bacteriophages as Models for Viral Aerosol Studies</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Turgeon</surname>
<given-names>Nathalie</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Toulouse</surname>
<given-names>Marie-Josée</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Martel</surname>
<given-names>Bruno</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Moineau</surname>
<given-names>Sylvain</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Duchaine</surname>
<given-names>Caroline</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<aff id="aff1"><label>a</label>
Institut Universitaire de Cardiologie et de Pneumologie de Québec, Hôpital Laval, Sainte-Foy, Quebec City, Quebec, Canada</aff>
<aff id="aff2"><label>b</label>
Département de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des Sciences et Génie, Université Laval, Quebec City, Quebec, Canada</aff>
<aff id="aff3"><label>c</label>
Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada</aff>
</contrib-group>
<contrib-group><contrib contrib-type="editor"><name><surname>Yates</surname>
<given-names>M. V.</given-names>
</name>
<role>Editor</role>
</contrib>
</contrib-group>
<author-notes><corresp id="cor1">Address correspondence to Caroline Duchaine, <email>Caroline.Duchaine@bcm.ulaval.ca</email>
.</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>7</month>
<year>2014</year>
</pub-date>
<volume>80</volume>
<issue>14</issue>
<fpage>4242</fpage>
<lpage>4250</lpage>
<history><date date-type="received"><day>11</day>
<month>3</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>29</day>
<month>4</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2014, American Society for Microbiology. All Rights Reserved.</copyright-statement>
<copyright-year>2014</copyright-year>
<copyright-holder>American Society for Microbiology</copyright-holder>
</permissions>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zam01414004242.pdf"></self-uri>
<abstract><p>Bacteriophages are perceived to be good models for the study of airborne viruses because they are safe to use, some of them display structural features similar to those of human and animal viruses, and they are relatively easy to produce in large quantities. Yet, only a few studies have investigated them as models. It has previously been demonstrated that aerosolization, environmental conditions, and sampling conditions affect viral infectivity, but viral infectivity is virus dependent. Thus, several virus models are likely needed to study their general behavior in aerosols. The aim of this study was to compare the effects of aerosolization and sampling on the infectivity of five tail-less bacteriophages and two pathogenic viruses: MS2 (a single-stranded RNA [ssRNA] phage of the <named-content content-type="genus-species">Leviviridae</named-content>
family), Φ6 (a segmented double-stranded RNA [dsRNA] phage of the <named-content content-type="genus-species">Cystoviridae</named-content>
family), ΦX174 (a single-stranded DNA [ssDNA] phage of the <named-content content-type="genus-species">Microviridae</named-content>
family), PM2 (a double-stranded DNA [dsDNA] phage of the <named-content content-type="genus-species">Corticoviridae</named-content>
family), PR772 (a dsDNA phage of the <named-content content-type="genus-species">Tectiviridae</named-content>
family), human influenza A virus H1N1 (an ssRNA virus of the <named-content content-type="genus-species">Orthomyxoviridae</named-content>
family), and the poultry virus Newcastle disease virus (NDV; an ssRNA virus of the <named-content content-type="genus-species">Paramyxoviridae</named-content>
family). Three nebulizers and two nebulization salt buffers (with or without organic fluid) were tested, as were two aerosol sampling devices, a liquid cyclone (SKC BioSampler) and a dry cyclone (National Institute for Occupational Safety and Health two-stage cyclone bioaerosol sampler). The presence of viruses in collected air samples was detected by culture and quantitative PCR (qPCR). Our results showed that these selected five phages behave differently when aerosolized and sampled. RNA phage MS2 and ssDNA phage ΦX174 were the most resistant to aerosolization and sampling. The presence of organic fluid in the nebulization buffer protected phages PR772 and Φ6 throughout the aerosolization and sampling with dry cyclones. In this experimental setup, the behavior of the influenza virus resembled that of phages PR772 and Φ6, while the behavior of NDV was closer to that of phages MS2 and ΦX174. These results provide critical information for the selection of appropriate phage models to mimic the behavior of specific human and animal viruses in aerosols.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>
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