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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Secretoneurin stimulates the production and release of luteinizing hormone in mouse LβT2 gonadotropin cells</title><author><name sortKey="Zhao, E" sort="Zhao, E" uniqKey="Zhao E" first="E." last="Zhao">E. Zhao</name><affiliation><nlm:aff id="aff1">Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada;</nlm:aff></affiliation></author><author><name sortKey="Mcneilly, Judy R" sort="Mcneilly, Judy R" uniqKey="Mcneilly J" first="Judy R." last="Mcneilly">Judy R. Mcneilly</name><affiliation><nlm:aff id="aff2">University of Edinburgh, Medical Research Council Human Reproductive Sciences Unit, the Queen's Medical Research Institute, Edinburgh, United Kingdom;</nlm:aff></affiliation></author><author><name sortKey="Mcneilly, Alan S" sort="Mcneilly, Alan S" uniqKey="Mcneilly A" first="Alan S." last="Mcneilly">Alan S. Mcneilly</name><affiliation><nlm:aff id="aff2">University of Edinburgh, Medical Research Council Human Reproductive Sciences Unit, the Queen's Medical Research Institute, Edinburgh, United Kingdom;</nlm:aff></affiliation></author><author><name sortKey="Fischer Colbrie, Reiner" sort="Fischer Colbrie, Reiner" uniqKey="Fischer Colbrie R" first="Reiner" last="Fischer-Colbrie">Reiner Fischer-Colbrie</name><affiliation><nlm:aff id="aff3">Department of Pharmacology, Innsbruck Medical University, Innsbruck, Austria;</nlm:aff></affiliation></author><author><name sortKey="Basak, Ajoy" sort="Basak, Ajoy" uniqKey="Basak A" first="Ajoy" last="Basak">Ajoy Basak</name><affiliation><nlm:aff wicri:cut="; and" id="aff4">Chronic Diseases Program, Ottawa Health Research Institute, Ottawa, Ontario, Canada</nlm:aff></affiliation></author><author><name sortKey="Seong, Jae Young" sort="Seong, Jae Young" uniqKey="Seong J" first="Jae Young" last="Seong">Jae Young Seong</name><affiliation><nlm:aff id="aff5">Graduate School of Medicine, Korea University, Seoul, South Korea</nlm:aff></affiliation></author><author><name sortKey="Trudeau, Vance L" sort="Trudeau, Vance L" uniqKey="Trudeau V" first="Vance L." last="Trudeau">Vance L. Trudeau</name><affiliation><nlm:aff id="aff1">Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada;</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21521715</idno><idno type="pmc">3154532</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154532</idno><idno type="RBID">PMC:3154532</idno><idno type="doi">10.1152/ajpendo.00070.2011</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">000673</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000673</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Secretoneurin stimulates the production and release of luteinizing hormone in mouse LβT2 gonadotropin cells</title><author><name sortKey="Zhao, E" sort="Zhao, E" uniqKey="Zhao E" first="E." last="Zhao">E. Zhao</name><affiliation><nlm:aff id="aff1">Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada;</nlm:aff></affiliation></author><author><name sortKey="Mcneilly, Judy R" sort="Mcneilly, Judy R" uniqKey="Mcneilly J" first="Judy R." last="Mcneilly">Judy R. Mcneilly</name><affiliation><nlm:aff id="aff2">University of Edinburgh, Medical Research Council Human Reproductive Sciences Unit, the Queen's Medical Research Institute, Edinburgh, United Kingdom;</nlm:aff></affiliation></author><author><name sortKey="Mcneilly, Alan S" sort="Mcneilly, Alan S" uniqKey="Mcneilly A" first="Alan S." last="Mcneilly">Alan S. Mcneilly</name><affiliation><nlm:aff id="aff2">University of Edinburgh, Medical Research Council Human Reproductive Sciences Unit, the Queen's Medical Research Institute, Edinburgh, United Kingdom;</nlm:aff></affiliation></author><author><name sortKey="Fischer Colbrie, Reiner" sort="Fischer Colbrie, Reiner" uniqKey="Fischer Colbrie R" first="Reiner" last="Fischer-Colbrie">Reiner Fischer-Colbrie</name><affiliation><nlm:aff id="aff3">Department of Pharmacology, Innsbruck Medical University, Innsbruck, Austria;</nlm:aff></affiliation></author><author><name sortKey="Basak, Ajoy" sort="Basak, Ajoy" uniqKey="Basak A" first="Ajoy" last="Basak">Ajoy Basak</name><affiliation><nlm:aff wicri:cut="; and" id="aff4">Chronic Diseases Program, Ottawa Health Research Institute, Ottawa, Ontario, Canada</nlm:aff></affiliation></author><author><name sortKey="Seong, Jae Young" sort="Seong, Jae Young" uniqKey="Seong J" first="Jae Young" last="Seong">Jae Young Seong</name><affiliation><nlm:aff id="aff5">Graduate School of Medicine, Korea University, Seoul, South Korea</nlm:aff></affiliation></author><author><name sortKey="Trudeau, Vance L" sort="Trudeau, Vance L" uniqKey="Trudeau V" first="Vance L." last="Trudeau">Vance L. Trudeau</name><affiliation><nlm:aff id="aff1">Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada;</nlm:aff></affiliation></author></analytic><series><title level="j">American Journal of Physiology - Endocrinology and Metabolism</title><idno type="ISSN">0193-1849</idno><idno type="eISSN">1522-1555</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Secretoneurin (SN) is a functional secretogranin II (SgII)-derived peptide that stimulates luteinizing hormone (LH) production and its release in the goldfish. However, the effects of SN on the pituitary of mammalian species and the underlying mechanisms remain poorly understood. To study SN in mammals, we adopted the mouse LβT2 gonadotropin cell line that has characteristics consistent with normal pituitary gonadotrophs. Using radioimmunoassay and real-time RT-PCR, we demonstrated that static treatment with SN induced a significant increment of LH release and production in LβT2 cells in vitro. We found that GnRH increased cellular SgII mRNA level and total SN-immunoreactive protein release into the culture medium. We also report that SN activated the extracellular signal-regulated kinases (ERK) in either 10-min acute stimulation or 3-h chronic treatment. The SN-induced ERK activation was significantly blocked by pharmacological inhibition of MAPK kinase (MEK) with PD-98059 and protein kinase C (PKC) with bisindolylmaleimide. SN also increased the total cyclic adenosine monophosphate (cAMP) levels similarly to GnRH. However, SN did not activate the GnRH receptor. These data indicate that SN activates the protein kinase A (PKA) and cAMP-induced ERK signaling pathways in the LH-secreting mouse LβT2 pituitary cell line.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Am J Physiol Endocrinol Metab</journal-id><journal-id journal-id-type="hwp">ajpendo</journal-id><journal-id journal-id-type="pmc">ajpendo</journal-id><journal-id journal-id-type="publisher-id">AJPENDO</journal-id><journal-title-group><journal-title>American Journal of Physiology - Endocrinology and Metabolism</journal-title></journal-title-group><issn pub-type="ppub">0193-1849</issn><issn pub-type="epub">1522-1555</issn><publisher><publisher-name>American Physiological Society</publisher-name><publisher-loc>Bethesda, MD</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">21521715</article-id><article-id pub-id-type="pmc">3154532</article-id><article-id pub-id-type="publisher-id">E-00070-2011</article-id><article-id pub-id-type="doi">10.1152/ajpendo.00070.2011</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>Secretoneurin stimulates the production and release of luteinizing hormone in mouse LβT2 gonadotropin cells</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zhao</surname><given-names>E.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>McNeilly</surname><given-names>Judy R.</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>McNeilly</surname><given-names>Alan S.</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Fischer-Colbrie</surname><given-names>Reiner</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Basak</surname><given-names>Ajoy</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Seong</surname><given-names>Jae Young</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Trudeau</surname><given-names>Vance L.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><aff id="aff1"><sup>1</sup>Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada;</aff><aff id="aff2"><sup>2</sup>University of Edinburgh, Medical Research Council Human Reproductive Sciences Unit, the Queen's Medical Research Institute, Edinburgh, United Kingdom;</aff><aff id="aff3"><sup>3</sup>Department of Pharmacology, Innsbruck Medical University, Innsbruck, Austria;</aff><aff id="aff4"><sup>4</sup>Chronic Diseases Program, Ottawa Health Research Institute, Ottawa, Ontario, Canada; and</aff><aff id="aff5"><sup>5</sup>Graduate School of Medicine, Korea University, Seoul, South Korea</aff></contrib-group><author-notes><corresp>Address for reprint requests and other correspondence: V. L. Trudeau, <addr-line>Dept. of Biology, Univ. of Ottawa, Gendron Hall, Rm. 160, 30 Marie Curie, Ottawa, ON, Canada K1N 6N5</addr-line> (e-mail: <email>trudeauv@uottawa.ca</email>).</corresp></author-notes><pub-date pub-type="ppub"><month>8</month><year>2011</year></pub-date><pub-date pub-type="epub"><day>26</day><month>4</month><year>2011</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>8</month><year>2012</year></pub-date><pmc-comment> PMC Release delay is 12 months and 0 days and was based on the
<volume>301</volume><issue>2</issue><fpage>E288</fpage><lpage>E297</lpage><history><date date-type="received"><day>14</day><month>2</month><year>2011</year></date><date date-type="accepted"><day>22</day><month>4</month><year>2011</year></date></history><permissions><copyright-statement>Copyright © 2011 the American Physiological Society</copyright-statement><copyright-year>2011</copyright-year></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zh100811000288.pdf"></self-uri><abstract><p>Secretoneurin (SN) is a functional secretogranin II (SgII)-derived peptide that stimulates luteinizing hormone (LH) production and its release in the goldfish. However, the effects of SN on the pituitary of mammalian species and the underlying mechanisms remain poorly understood. To study SN in mammals, we adopted the mouse LβT2 gonadotropin cell line that has characteristics consistent with normal pituitary gonadotrophs. Using radioimmunoassay and real-time RT-PCR, we demonstrated that static treatment with SN induced a significant increment of LH release and production in LβT2 cells in vitro. We found that GnRH increased cellular SgII mRNA level and total SN-immunoreactive protein release into the culture medium. We also report that SN activated the extracellular signal-regulated kinases (ERK) in either 10-min acute stimulation or 3-h chronic treatment. The SN-induced ERK activation was significantly blocked by pharmacological inhibition of MAPK kinase (MEK) with PD-98059 and protein kinase C (PKC) with bisindolylmaleimide. SN also increased the total cyclic adenosine monophosphate (cAMP) levels similarly to GnRH. However, SN did not activate the GnRH receptor. These data indicate that SN activates the protein kinase A (PKA) and cAMP-induced ERK signaling pathways in the LH-secreting mouse LβT2 pituitary cell line.</p></abstract><kwd-group><kwd>secretogranin II</kwd><kwd>gonadotropin-releasing hormone</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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