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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Experimental reproduction of Potomac horse fever in horses with a newly isolated Ehrlichia organism.</title><author><name sortKey="Dutta, S K" sort="Dutta, S K" uniqKey="Dutta S" first="S K" last="Dutta">S K Dutta</name></author><author><name sortKey="Myrup, A C" sort="Myrup, A C" uniqKey="Myrup A" first="A C" last="Myrup">A C Myrup</name></author><author><name sortKey="Rice, R M" sort="Rice, R M" uniqKey="Rice R" first="R M" last="Rice">R M Rice</name></author><author><name sortKey="Robl, M G" sort="Robl, M G" uniqKey="Robl M" first="M G" last="Robl">M G Robl</name></author><author><name sortKey="Hammond, R C" sort="Hammond, R C" uniqKey="Hammond R" first="R C" last="Hammond">R C Hammond</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">4031040</idno><idno type="pmc">268372</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC268372</idno><idno type="RBID">PMC:268372</idno><date when="1985">1985</date><idno type="wicri:Area/Pmc/Corpus">000461</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000461</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Experimental reproduction of Potomac horse fever in horses with a newly isolated Ehrlichia organism.</title><author><name sortKey="Dutta, S K" sort="Dutta, S K" uniqKey="Dutta S" first="S K" last="Dutta">S K Dutta</name></author><author><name sortKey="Myrup, A C" sort="Myrup, A C" uniqKey="Myrup A" first="A C" last="Myrup">A C Myrup</name></author><author><name sortKey="Rice, R M" sort="Rice, R M" uniqKey="Rice R" first="R M" last="Rice">R M Rice</name></author><author><name sortKey="Robl, M G" sort="Robl, M G" uniqKey="Robl M" first="M G" last="Robl">M G Robl</name></author><author><name sortKey="Hammond, R C" sort="Hammond, R C" uniqKey="Hammond R" first="R C" last="Hammond">R C Hammond</name></author></analytic><series><title level="j">Journal of Clinical Microbiology</title><idno type="ISSN">0095-1137</idno><idno type="eISSN">1098-660X</idno><imprint><date when="1985">1985</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Potomac horse fever, a recently recognized disease of equines, characterized by high fever, leukopenia, and a profuse diarrhea, was studied for its etiology. An Ehrlichia organism was isolated in equine macrophage-fibroblast cell cultures and mouse macrophage cell cultures from the mononuclear cells of blood of infected horses. The agent was continuously propagated in mouse macrophage cell cultures. The organism multiplied in the cytoplasm of mouse macrophage cells and was identified by Giemsa staining, acridine orange staining, and by indirect immunofluorescence with convalescent sera from infected horses. The disease was experimentally reproduced in horses inoculated with Ehrlichia-infected cell culture material. The Ehrlichia organism was reisolated from the blood of these infected horses during the course of the disease. Antibody against the organism was detected in the sera of experimentally infected horses. This study confirmed that the new Ehrlichia organism is the etiological agent of Potomac horse fever.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="jcm00109-0150-a" xlink:role="266"></graphic></fig><fig id="F2"><graphic xlink:href="jcm00109-0151-a" xlink:role="267"></graphic></fig><fig id="F3"><graphic xlink:href="jcm00109-0151-b" xlink:role="267"></graphic></fig></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id><journal-title>Journal of Clinical Microbiology</journal-title><issn pub-type="ppub">0095-1137</issn><issn pub-type="epub">1098-660X</issn></journal-meta><article-meta><article-id pub-id-type="pmid">4031040</article-id><article-id pub-id-type="pmc">268372</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Experimental reproduction of Potomac horse fever in horses with a newly isolated Ehrlichia organism.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Dutta</surname><given-names>S K</given-names></name></contrib><contrib contrib-type="author"><name><surname>Myrup</surname><given-names>A C</given-names></name></contrib><contrib contrib-type="author"><name><surname>Rice</surname><given-names>R M</given-names></name></contrib><contrib contrib-type="author"><name><surname>Robl</surname><given-names>M G</given-names></name></contrib><contrib contrib-type="author"><name><surname>Hammond</surname><given-names>R C</given-names></name></contrib></contrib-group><pub-date pub-type="ppub"><month>8</month><year>1985</year></pub-date><volume>22</volume><issue>2</issue><fpage>265</fpage><lpage>269</lpage><abstract><p>Potomac horse fever, a recently recognized disease of equines, characterized by high fever, leukopenia, and a profuse diarrhea, was studied for its etiology. An Ehrlichia organism was isolated in equine macrophage-fibroblast cell cultures and mouse macrophage cell cultures from the mononuclear cells of blood of infected horses. The agent was continuously propagated in mouse macrophage cell cultures. The organism multiplied in the cytoplasm of mouse macrophage cells and was identified by Giemsa staining, acridine orange staining, and by indirect immunofluorescence with convalescent sera from infected horses. The disease was experimentally reproduced in horses inoculated with Ehrlichia-infected cell culture material. The Ehrlichia organism was reisolated from the blood of these infected horses during the course of the disease. Antibody against the organism was detected in the sera of experimentally infected horses. This study confirmed that the new Ehrlichia organism is the etiological agent of Potomac horse fever.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="jcm00109-0150-a" xlink:role="266"></graphic></fig><fig id="F2"><graphic xlink:href="jcm00109-0151-a" xlink:role="267"></graphic></fig><fig id="F3"><graphic xlink:href="jcm00109-0151-b" xlink:role="267"></graphic></fig></sec></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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