Highly conserved regions in Ebola virus RNA dependent RNA polymerase may be act as a universal novel peptide vaccine target: a computational approach
Identifieur interne : 000266 ( Pmc/Corpus ); précédent : 000265; suivant : 000267Highly conserved regions in Ebola virus RNA dependent RNA polymerase may be act as a universal novel peptide vaccine target: a computational approach
Auteurs : Arafat Rahman Oany ; Tahmina Sharmin ; Afrin Sultana Chowdhury ; Tahmina Pervin Jyoti ; Md. Anayet HasanSource :
- In Silico Pharmacology [ 2193-9616 ] ; 2015.
Abstract
Ebola virus (EBOV) is such kind of virus which is responsible for 23,825 cases and 9675 deaths worldwide only in 2014 and with an average diseases fatality rate between 25 % and 90 %. Although, medical technology has tried to handle the problems, there is no Food and Drug Administration (FDA)-approved therapeutics or vaccines available for the prevention, post exposure, or treatment of Ebola virus disease (EVD).
In the present study, we used the immunoinformatics approach to design a potential epitope-based vaccine against the RNA-dependent RNA polymerase-L of EBOV. BioEdit v7.2.3 sequence alignment editor, Jalview v2 and CLC Sequence Viewer v7.0.2 were used for the initial sequence analysis for securing the conservancy from the sequences. Later the Immune Epitope Database and Analysis Resource (IEDB-AR) was used for the identification of T-cell and B-cellepitopes associated with type I and II major histocompatibility complex molecules analysis. Finally, the population coverage analysis was employed.
The core epitope “FRYEFTAPF” was found to be the most potential one, with 100 % conservancy among all the strains of EBOV. It also interacted with both type I and II major histocompatibility complex molecules and is considered as nonallergenic in nature. Finally, with impressive cumulative population coverage of 99.87 % for the both MHC-I and MHC-II class throughout the world population was found for the proposed epitope.
To end, the projected peptide gave us a solid stand to propose for vaccine consideration and that might be experimented for its potency in eliciting immunity through humoral and cell mediated immune responses
The online version of this article (doi:10.1186/s40203-015-0011-4) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s40203-015-0011-4
PubMed: 26820892
PubMed Central: 4529428
Links to Exploration step
PMC:4529428Le document en format XML
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<author><name sortKey="Oany, Arafat Rahman" sort="Oany, Arafat Rahman" uniqKey="Oany A" first="Arafat Rahman" last="Oany">Arafat Rahman Oany</name>
<affiliation><nlm:aff id="Aff1">Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh</nlm:aff>
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<author><name sortKey="Sharmin, Tahmina" sort="Sharmin, Tahmina" uniqKey="Sharmin T" first="Tahmina" last="Sharmin">Tahmina Sharmin</name>
<affiliation><nlm:aff id="Aff1">Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh</nlm:aff>
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<author><name sortKey="Chowdhury, Afrin Sultana" sort="Chowdhury, Afrin Sultana" uniqKey="Chowdhury A" first="Afrin Sultana" last="Chowdhury">Afrin Sultana Chowdhury</name>
<affiliation><nlm:aff id="Aff2">Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh</nlm:aff>
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<author><name sortKey="Jyoti, Tahmina Pervin" sort="Jyoti, Tahmina Pervin" uniqKey="Jyoti T" first="Tahmina Pervin" last="Jyoti">Tahmina Pervin Jyoti</name>
<affiliation><nlm:aff id="Aff3">Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh</nlm:aff>
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<author><name sortKey="Hasan, Md Anayet" sort="Hasan, Md Anayet" uniqKey="Hasan M" first="Md. Anayet" last="Hasan">Md. Anayet Hasan</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Highly conserved regions in Ebola virus RNA dependent RNA polymerase may be act as a universal novel peptide vaccine target: a computational approach</title>
<author><name sortKey="Oany, Arafat Rahman" sort="Oany, Arafat Rahman" uniqKey="Oany A" first="Arafat Rahman" last="Oany">Arafat Rahman Oany</name>
<affiliation><nlm:aff id="Aff1">Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh</nlm:aff>
</affiliation>
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<author><name sortKey="Sharmin, Tahmina" sort="Sharmin, Tahmina" uniqKey="Sharmin T" first="Tahmina" last="Sharmin">Tahmina Sharmin</name>
<affiliation><nlm:aff id="Aff1">Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh</nlm:aff>
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<author><name sortKey="Chowdhury, Afrin Sultana" sort="Chowdhury, Afrin Sultana" uniqKey="Chowdhury A" first="Afrin Sultana" last="Chowdhury">Afrin Sultana Chowdhury</name>
<affiliation><nlm:aff id="Aff2">Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh</nlm:aff>
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<author><name sortKey="Jyoti, Tahmina Pervin" sort="Jyoti, Tahmina Pervin" uniqKey="Jyoti T" first="Tahmina Pervin" last="Jyoti">Tahmina Pervin Jyoti</name>
<affiliation><nlm:aff id="Aff3">Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh</nlm:aff>
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<author><name sortKey="Hasan, Md Anayet" sort="Hasan, Md Anayet" uniqKey="Hasan M" first="Md. Anayet" last="Hasan">Md. Anayet Hasan</name>
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<series><title level="j">In Silico Pharmacology</title>
<idno type="eISSN">2193-9616</idno>
<imprint><date when="2015">2015</date>
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<front><div type="abstract" xml:lang="en"><sec><title>Purpose</title>
<p>Ebola virus (EBOV) is such kind of virus which is responsible for 23,825 cases and 9675 deaths worldwide only in 2014 and with an average diseases fatality rate between 25 % and 90 %. Although, medical technology has tried to handle the problems, there is no Food and Drug Administration (FDA)-approved therapeutics or vaccines available for the prevention, post exposure, or treatment of Ebola virus disease (EVD).</p>
</sec>
<sec><title>Methods</title>
<p>In the present study, we used the immunoinformatics approach to design a potential epitope-based vaccine against the RNA-dependent RNA polymerase-L of EBOV. BioEdit v7.2.3 sequence alignment editor, Jalview v2 and CLC Sequence Viewer v7.0.2 were used for the initial sequence analysis for securing the conservancy from the sequences. Later the Immune Epitope Database and Analysis Resource (IEDB-AR) was used for the identification of T-cell and B-cellepitopes associated with type I and II major histocompatibility complex molecules analysis. Finally, the population coverage analysis was employed.</p>
</sec>
<sec><title>Results</title>
<p>The core epitope “FRYEFTAPF” was found to be the most potential one, with 100 % conservancy among all the strains of EBOV. It also interacted with both type I and II major histocompatibility complex molecules and is considered as nonallergenic in nature. Finally, with impressive cumulative population coverage of 99.87 % for the both MHC-I and MHC-II class throughout the world population was found for the proposed epitope.</p>
</sec>
<sec><title>Conclusion</title>
<p>To end, the projected peptide gave us a solid stand to propose for vaccine consideration and that might be experimented for its potency in eliciting immunity through humoral and cell mediated immune responses <italic>in vitro</italic>
and <italic>in vivo</italic>
.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s40203-015-0011-4) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Apweiler, R" uniqKey="Apweiler R">R Apweiler</name>
</author>
<author><name sortKey="Bairoch, A" uniqKey="Bairoch A">A Bairoch</name>
</author>
<author><name sortKey="Wu, Ch" uniqKey="Wu C">CH Wu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Arnold, K" uniqKey="Arnold K">K Arnold</name>
</author>
<author><name sortKey="Bordoli, L" uniqKey="Bordoli L">L Bordoli</name>
</author>
<author><name sortKey="Kopp, J" uniqKey="Kopp J">J Kopp</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bacchetta, R" uniqKey="Bacchetta R">R Bacchetta</name>
</author>
<author><name sortKey="Gregori, S" uniqKey="Gregori S">S Gregori</name>
</author>
<author><name sortKey="Roncarolo, M G" uniqKey="Roncarolo M">M-G Roncarolo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Baize, S" uniqKey="Baize S">S Baize</name>
</author>
<author><name sortKey="Pannetier, D" uniqKey="Pannetier D">D Pannetier</name>
</author>
<author><name sortKey="Oestereich, L" uniqKey="Oestereich L">L Oestereich</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Benkert, P" uniqKey="Benkert P">P Benkert</name>
</author>
<author><name sortKey="Biasini, M" uniqKey="Biasini M">M Biasini</name>
</author>
<author><name sortKey="Schwede, T" uniqKey="Schwede T">T Schwede</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bhasin, M" uniqKey="Bhasin M">M Bhasin</name>
</author>
<author><name sortKey="Raghava, G" uniqKey="Raghava G">G Raghava</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bourdette, Dn" uniqKey="Bourdette D">DN Bourdette</name>
</author>
<author><name sortKey="Edmonds, E" uniqKey="Edmonds E">E Edmonds</name>
</author>
<author><name sortKey="Smith, C" uniqKey="Smith C">C Smith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bui, Hh" uniqKey="Bui H">HH Bui</name>
</author>
<author><name sortKey="Sidney, J" uniqKey="Sidney J">J Sidney</name>
</author>
<author><name sortKey="Dinh, K" uniqKey="Dinh K">K Dinh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bui, Hh" uniqKey="Bui H">HH Bui</name>
</author>
<author><name sortKey="Sidney, J" uniqKey="Sidney J">J Sidney</name>
</author>
<author><name sortKey="Li, W" uniqKey="Li W">W Li</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Choi, Jh" uniqKey="Choi J">JH Choi</name>
</author>
<author><name sortKey="Croyle, Ma" uniqKey="Croyle M">MA Croyle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chou, Py" uniqKey="Chou P">PY Chou</name>
</author>
<author><name sortKey="Fasman, Gd" uniqKey="Fasman G">GD Fasman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chowell, G" uniqKey="Chowell G">G Chowell</name>
</author>
<author><name sortKey="Hengartner, Nw" uniqKey="Hengartner N">NW Hengartner</name>
</author>
<author><name sortKey="Castillo Chavez, C" uniqKey="Castillo Chavez C">C Castillo-Chavez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crary, S" uniqKey="Crary S">S Crary</name>
</author>
<author><name sortKey="Towner, J" uniqKey="Towner J">J Towner</name>
</author>
<author><name sortKey="Honig, J" uniqKey="Honig J">J Honig</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Doytchinova, Ia" uniqKey="Doytchinova I">IA Doytchinova</name>
</author>
<author><name sortKey="Flower, Dr" uniqKey="Flower D">DR Flower</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Elisha, A" uniqKey="Elisha A">A Elisha</name>
</author>
<author><name sortKey="Adegboro, B" uniqKey="Adegboro B">B Adegboro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Emini, Ea" uniqKey="Emini E">EA Emini</name>
</author>
<author><name sortKey="Hughes, Jv" uniqKey="Hughes J">JV Hughes</name>
</author>
<author><name sortKey="Perlow, Ds" uniqKey="Perlow D">DS Perlow</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Evans, Dk" uniqKey="Evans D">DK Evans</name>
</author>
<author><name sortKey="Popova, A" uniqKey="Popova A">A Popova</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H Feldmann</name>
</author>
<author><name sortKey="Volchkov, Ve" uniqKey="Volchkov V">VE Volchkov</name>
</author>
<author><name sortKey="Volchkova, Va" uniqKey="Volchkova V">VA Volchkova</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H Feldmann</name>
</author>
<author><name sortKey="Volchkov, V" uniqKey="Volchkov V">V Volchkov</name>
</author>
<author><name sortKey="Stroher, U" uniqKey="Stroher U">U Ströher</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H Feldmann</name>
</author>
<author><name sortKey="Jones, S" uniqKey="Jones S">S Jones</name>
</author>
<author><name sortKey="Klenk, Hd" uniqKey="Klenk H">HD Klenk</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fiser, A" uniqKey="Fiser A">A Fiser</name>
</author>
<author><name sortKey="Do, Rk" uniqKey="Do R">RK Do</name>
</author>
<author><name sortKey="Sali, A" uniqKey="Sali A">A Sali</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Frieden, Tr" uniqKey="Frieden T">TR Frieden</name>
</author>
<author><name sortKey="Damon, I" uniqKey="Damon I">I Damon</name>
</author>
<author><name sortKey="Bell, Bp" uniqKey="Bell B">BP Bell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Garcia, Kc" uniqKey="Garcia K">KC Garcia</name>
</author>
<author><name sortKey="Teyton, L" uniqKey="Teyton L">L Teyton</name>
</author>
<author><name sortKey="Wilson, Ia" uniqKey="Wilson I">IA Wilson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Garcia Boronat, M" uniqKey="Garcia Boronat M">M Garcia-Boronat</name>
</author>
<author><name sortKey="Diez Rivero, Cm" uniqKey="Diez Rivero C">CM Diez-Rivero</name>
</author>
<author><name sortKey="Reinherz, El" uniqKey="Reinherz E">EL Reinherz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gatherer, D" uniqKey="Gatherer D">D Gatherer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Geisbert, Tw" uniqKey="Geisbert T">TW Geisbert</name>
</author>
<author><name sortKey="Geisbert, Jb" uniqKey="Geisbert J">JB Geisbert</name>
</author>
<author><name sortKey="Leung, A" uniqKey="Leung A">A Leung</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Germain, Rn" uniqKey="Germain R">RN Germain</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Goeijenbier, M" uniqKey="Goeijenbier M">M Goeijenbier</name>
</author>
<author><name sortKey="Van Kampen, Jj" uniqKey="Van Kampen J">JJ van Kampen</name>
</author>
<author><name sortKey="Reusken, Cb" uniqKey="Reusken C">CB Reusken</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hall, Ta" uniqKey="Hall T">TA Hall</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hasan, Ma" uniqKey="Hasan M">MA Hasan</name>
</author>
<author><name sortKey="Hossain, M" uniqKey="Hossain M">M Hossain</name>
</author>
<author><name sortKey="Alam, Mj" uniqKey="Alam M">MJ Alam</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hasan, Ma" uniqKey="Hasan M">MA Hasan</name>
</author>
<author><name sortKey="Khan, Ma" uniqKey="Khan M">MA Khan</name>
</author>
<author><name sortKey="Datta, A" uniqKey="Datta A">A Datta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hewlett, Bl" uniqKey="Hewlett B">BL Hewlett</name>
</author>
<author><name sortKey="Hewlett, Bs" uniqKey="Hewlett B">BS Hewlett</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hoenen, T" uniqKey="Hoenen T">T Hoenen</name>
</author>
<author><name sortKey="Groseth, A" uniqKey="Groseth A">A Groseth</name>
</author>
<author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H Feldmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Holland, J" uniqKey="Holland J">J Holland</name>
</author>
<author><name sortKey="Domingo, E" uniqKey="Domingo E">E Domingo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hoof, I" uniqKey="Hoof I">I Hoof</name>
</author>
<author><name sortKey="Peters, B" uniqKey="Peters B">B Peters</name>
</author>
<author><name sortKey="Sidney, J" uniqKey="Sidney J">J Sidney</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Igietseme, Ju" uniqKey="Igietseme J">JU Igietseme</name>
</author>
<author><name sortKey="Eko, Fo" uniqKey="Eko F">FO Eko</name>
</author>
<author><name sortKey="He, Q" uniqKey="He Q">Q He</name>
</author>
<author><name sortKey="Black, Cm" uniqKey="Black C">CM Black</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Keiser, J" uniqKey="Keiser J">J Keiser</name>
</author>
<author><name sortKey="Utzinger, J" uniqKey="Utzinger J">J Utzinger</name>
</author>
<author><name sortKey="Tanner, M" uniqKey="Tanner M">M Tanner</name>
</author>
<author><name sortKey="Singer, Bh" uniqKey="Singer B">BH Singer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Khan, Mk" uniqKey="Khan M">MK Khan</name>
</author>
<author><name sortKey="Zaman, S" uniqKey="Zaman S">S Zaman</name>
</author>
<author><name sortKey="Chakraborty, S" uniqKey="Chakraborty S">S Chakraborty</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Klein, Rs" uniqKey="Klein R">RS Klein</name>
</author>
<author><name sortKey="Lin, E" uniqKey="Lin E">E Lin</name>
</author>
<author><name sortKey="Zhang, B" uniqKey="Zhang B">B Zhang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kolaskar, As" uniqKey="Kolaskar A">AS Kolaskar</name>
</author>
<author><name sortKey="Tongaonkar, Pc" uniqKey="Tongaonkar P">PC Tongaonkar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kuhn, Jh" uniqKey="Kuhn J">JH Kuhn</name>
</author>
<author><name sortKey="Becker, S" uniqKey="Becker S">S Becker</name>
</author>
<author><name sortKey="Ebihara, H" uniqKey="Ebihara H">H Ebihara</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Larsen, Mv" uniqKey="Larsen M">MV Larsen</name>
</author>
<author><name sortKey="Lundegaard, C" uniqKey="Lundegaard C">C Lundegaard</name>
</author>
<author><name sortKey="Lamberth, K" uniqKey="Lamberth K">K Lamberth</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Laskowski, Ra" uniqKey="Laskowski R">RA Laskowski</name>
</author>
<author><name sortKey="Rullmann, Jac" uniqKey="Rullmann J">JAC Rullmann</name>
</author>
<author><name sortKey="Macarthur, Mw" uniqKey="Macarthur M">MW MacArthur</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Leroy, Em" uniqKey="Leroy E">EM Leroy</name>
</author>
<author><name sortKey="Rouquet, P" uniqKey="Rouquet P">P Rouquet</name>
</author>
<author><name sortKey="Formenty, P" uniqKey="Formenty P">P Formenty</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, Yh" uniqKey="Li Y">YH Li</name>
</author>
<author><name sortKey="Chen, Sp" uniqKey="Chen S">SP Chen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liao, L" uniqKey="Liao L">L Liao</name>
</author>
<author><name sortKey="Noble, Ws" uniqKey="Noble W">WS Noble</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="L Pez, Ja" uniqKey="L Pez J">JA López</name>
</author>
<author><name sortKey="Weilenman, C" uniqKey="Weilenman C">C Weilenman</name>
</author>
<author><name sortKey="Audran, R" uniqKey="Audran R">R Audran</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mckeever, Tm" uniqKey="Mckeever T">TM McKeever</name>
</author>
<author><name sortKey="Lewis, Sa" uniqKey="Lewis S">SA Lewis</name>
</author>
<author><name sortKey="Smith, C" uniqKey="Smith C">C Smith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Muh, Hc" uniqKey="Muh H">HC Muh</name>
</author>
<author><name sortKey="Tong, Jc" uniqKey="Tong J">JC Tong</name>
</author>
<author><name sortKey="Tammi, Mt" uniqKey="Tammi M">MT Tammi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Muhlberger, E" uniqKey="Muhlberger E">E Mühlberger</name>
</author>
<author><name sortKey="Lotfering, B" uniqKey="Lotfering B">B Lotfering</name>
</author>
<author><name sortKey="Klenk, H D" uniqKey="Klenk H">H-D Klenk</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Muhlberger, E" uniqKey="Muhlberger E">E Mühlberger</name>
</author>
<author><name sortKey="Weik, M" uniqKey="Weik M">M Weik</name>
</author>
<author><name sortKey="Volchkov, V" uniqKey="Volchkov V">V Volchkov</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nair, Dt" uniqKey="Nair D">DT Nair</name>
</author>
<author><name sortKey="Singh, K" uniqKey="Singh K">K Singh</name>
</author>
<author><name sortKey="Siddiqui, Z" uniqKey="Siddiqui Z">Z Siddiqui</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nielsen, M" uniqKey="Nielsen M">M Nielsen</name>
</author>
<author><name sortKey="Lundegaard, C" uniqKey="Lundegaard C">C Lundegaard</name>
</author>
<author><name sortKey="Blicher, T" uniqKey="Blicher T">T Blicher</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oany, Ar" uniqKey="Oany A">AR Oany</name>
</author>
<author><name sortKey="Emran, Aa" uniqKey="Emran A">AA Emran</name>
</author>
<author><name sortKey="Jyoti, Tp" uniqKey="Jyoti T">TP Jyoti</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oany, Ar" uniqKey="Oany A">AR Oany</name>
</author>
<author><name sortKey="Ahmad, Sai" uniqKey="Ahmad S">SAI Ahmad</name>
</author>
<author><name sortKey="Hossain, Mu" uniqKey="Hossain M">MU Hossain</name>
</author>
<author><name sortKey="Jyoti, Tp" uniqKey="Jyoti T">TP Jyoti</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Parker, Jm" uniqKey="Parker J">JM Parker</name>
</author>
<author><name sortKey="Guo, D" uniqKey="Guo D">D Guo</name>
</author>
<author><name sortKey="Hodges, Rs" uniqKey="Hodges R">RS Hodges</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Peters, Cj" uniqKey="Peters C">CJ Peters</name>
</author>
<author><name sortKey="Peters, Jw" uniqKey="Peters J">JW Peters</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Peters, B" uniqKey="Peters B">B Peters</name>
</author>
<author><name sortKey="Sette, A" uniqKey="Sette A">A Sette</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Petrovsky, N" uniqKey="Petrovsky N">N Petrovsky</name>
</author>
<author><name sortKey="Brusic, V" uniqKey="Brusic V">V Brusic</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Poch, O" uniqKey="Poch O">O Poch</name>
</author>
<author><name sortKey="Blumberg, Bm" uniqKey="Blumberg B">BM Blumberg</name>
</author>
<author><name sortKey="Bougueleret, L" uniqKey="Bougueleret L">L Bougueleret</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Poland, Ga" uniqKey="Poland G">GA Poland</name>
</author>
<author><name sortKey="Ovsyannikova, Ig" uniqKey="Ovsyannikova I">IG Ovsyannikova</name>
</author>
<author><name sortKey="Jacobson, Rm" uniqKey="Jacobson R">RM Jacobson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rini, Jm" uniqKey="Rini J">JM Rini</name>
</author>
<author><name sortKey="Schulze Gahmen, U" uniqKey="Schulze Gahmen U">U Schulze-Gahmen</name>
</author>
<author><name sortKey="Wilson, Ia" uniqKey="Wilson I">IA Wilson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Robinson, Hl" uniqKey="Robinson H">HL Robinson</name>
</author>
<author><name sortKey="Amara, Rr" uniqKey="Amara R">RR Amara</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rouquet, P" uniqKey="Rouquet P">P Rouquet</name>
</author>
<author><name sortKey="Froment, Jm" uniqKey="Froment J">JM Froment</name>
</author>
<author><name sortKey="Bermejo, M" uniqKey="Bermejo M">M Bermejo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sali, A" uniqKey="Sali A">A Sali</name>
</author>
<author><name sortKey="Blundell, Tl" uniqKey="Blundell T">TL Blundell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sali, A" uniqKey="Sali A">A Šali</name>
</author>
<author><name sortKey="Potterton, L" uniqKey="Potterton L">L Potterton</name>
</author>
<author><name sortKey="Yuan, F" uniqKey="Yuan F">F Yuan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sette, A" uniqKey="Sette A">A Sette</name>
</author>
<author><name sortKey="Fikes, J" uniqKey="Fikes J">J Fikes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sette, A" uniqKey="Sette A">A Sette</name>
</author>
<author><name sortKey="Newman, M" uniqKey="Newman M">M Newman</name>
</author>
<author><name sortKey="Livingston, B" uniqKey="Livingston B">B Livingston</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shrestha, B" uniqKey="Shrestha B">B Shrestha</name>
</author>
<author><name sortKey="Diamond, Ms" uniqKey="Diamond M">MS Diamond</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sullivan, Nj" uniqKey="Sullivan N">NJ Sullivan</name>
</author>
<author><name sortKey="Sanchez, A" uniqKey="Sanchez A">A Sanchez</name>
</author>
<author><name sortKey="Rollin, Pe" uniqKey="Rollin P">PE Rollin</name>
</author>
<author><name sortKey="Yang, Zy" uniqKey="Yang Z">ZY Yang</name>
</author>
<author><name sortKey="Nabel, Gj" uniqKey="Nabel G">GJ Nabel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Takada, A" uniqKey="Takada A">A Takada</name>
</author>
<author><name sortKey="Robison, C" uniqKey="Robison C">C Robison</name>
</author>
<author><name sortKey="Goto, H" uniqKey="Goto H">H Goto</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thompson, Jd" uniqKey="Thompson J">JD Thompson</name>
</author>
<author><name sortKey="Higgins, Dg" uniqKey="Higgins D">DG Higgins</name>
</author>
<author><name sortKey="Gibson, Tj" uniqKey="Gibson T">TJ Gibson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Trainor, Nb" uniqKey="Trainor N">NB Trainor</name>
</author>
<author><name sortKey="Crill, Wd" uniqKey="Crill W">WD Crill</name>
</author>
<author><name sortKey="Roberson, Ja" uniqKey="Roberson J">JA Roberson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Twiddy, Ss" uniqKey="Twiddy S">SS Twiddy</name>
</author>
<author><name sortKey="Holmes, Ec" uniqKey="Holmes E">EC Holmes</name>
</author>
<author><name sortKey="Rambaut, A" uniqKey="Rambaut A">A Rambaut</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Volchkov, Ve" uniqKey="Volchkov V">VE Volchkov</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, P" uniqKey="Wang P">P Wang</name>
</author>
<author><name sortKey="Sidney, J" uniqKey="Sidney J">J Sidney</name>
</author>
<author><name sortKey="Dow, C" uniqKey="Dow C">C Dow</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, P" uniqKey="Wang P">P Wang</name>
</author>
<author><name sortKey="Sidney, J" uniqKey="Sidney J">J Sidney</name>
</author>
<author><name sortKey="Kim, Y" uniqKey="Kim Y">Y Kim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ward, Jj" uniqKey="Ward J">JJ Ward</name>
</author>
<author><name sortKey="Mcguffin, Lj" uniqKey="Mcguffin L">LJ McGuffin</name>
</author>
<author><name sortKey="Bryson, K" uniqKey="Bryson K">K Bryson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Waterhouse, Am" uniqKey="Waterhouse A">AM Waterhouse</name>
</author>
<author><name sortKey="Procter, Jb" uniqKey="Procter J">JB Procter</name>
</author>
<author><name sortKey="Martin, Dm" uniqKey="Martin D">DM Martin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wilson, Cc" uniqKey="Wilson C">CC Wilson</name>
</author>
<author><name sortKey="Mckinney, D" uniqKey="Mckinney D">D McKinney</name>
</author>
<author><name sortKey="Anders, M" uniqKey="Anders M">M Anders</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">In Silico Pharmacol</journal-id>
<journal-id journal-id-type="iso-abbrev">In Silico Pharmacol</journal-id>
<journal-title-group><journal-title>In Silico Pharmacology</journal-title>
</journal-title-group>
<issn pub-type="epub">2193-9616</issn>
<publisher><publisher-name>Springer Berlin Heidelberg</publisher-name>
<publisher-loc>Berlin/Heidelberg</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">26820892</article-id>
<article-id pub-id-type="pmc">4529428</article-id>
<article-id pub-id-type="publisher-id">11</article-id>
<article-id pub-id-type="doi">10.1186/s40203-015-0011-4</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Research</subject>
</subj-group>
</article-categories>
<title-group><article-title>Highly conserved regions in Ebola virus RNA dependent RNA polymerase may be act as a universal novel peptide vaccine target: a computational approach</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Oany</surname>
<given-names>Arafat Rahman</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sharmin</surname>
<given-names>Tahmina</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Chowdhury</surname>
<given-names>Afrin Sultana</given-names>
</name>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jyoti</surname>
<given-names>Tahmina Pervin</given-names>
</name>
<xref ref-type="aff" rid="Aff3"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Hasan</surname>
<given-names>Md. Anayet</given-names>
</name>
<address><phone>+8801717344389</phone>
<email>anayet_johny@yahoo.com</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<aff id="Aff1"><label></label>
Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh</aff>
<aff id="Aff2"><label></label>
Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh</aff>
<aff id="Aff3"><label></label>
Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh</aff>
</contrib-group>
<pub-date pub-type="epub"><day>8</day>
<month>8</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>8</day>
<month>8</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="collection"><year>2015</year>
</pub-date>
<volume>3</volume>
<elocation-id>7</elocation-id>
<history><date date-type="received"><day>7</day>
<month>4</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>22</day>
<month>7</month>
<year>2015</year>
</date>
</history>
<permissions><copyright-statement>© Oany et al. 2015</copyright-statement>
<license license-type="OpenAccess"><license-p><bold>Open Access</bold>
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.</license-p>
</license>
</permissions>
<abstract id="Abs1"><sec><title>Purpose</title>
<p>Ebola virus (EBOV) is such kind of virus which is responsible for 23,825 cases and 9675 deaths worldwide only in 2014 and with an average diseases fatality rate between 25 % and 90 %. Although, medical technology has tried to handle the problems, there is no Food and Drug Administration (FDA)-approved therapeutics or vaccines available for the prevention, post exposure, or treatment of Ebola virus disease (EVD).</p>
</sec>
<sec><title>Methods</title>
<p>In the present study, we used the immunoinformatics approach to design a potential epitope-based vaccine against the RNA-dependent RNA polymerase-L of EBOV. BioEdit v7.2.3 sequence alignment editor, Jalview v2 and CLC Sequence Viewer v7.0.2 were used for the initial sequence analysis for securing the conservancy from the sequences. Later the Immune Epitope Database and Analysis Resource (IEDB-AR) was used for the identification of T-cell and B-cellepitopes associated with type I and II major histocompatibility complex molecules analysis. Finally, the population coverage analysis was employed.</p>
</sec>
<sec><title>Results</title>
<p>The core epitope “FRYEFTAPF” was found to be the most potential one, with 100 % conservancy among all the strains of EBOV. It also interacted with both type I and II major histocompatibility complex molecules and is considered as nonallergenic in nature. Finally, with impressive cumulative population coverage of 99.87 % for the both MHC-I and MHC-II class throughout the world population was found for the proposed epitope.</p>
</sec>
<sec><title>Conclusion</title>
<p>To end, the projected peptide gave us a solid stand to propose for vaccine consideration and that might be experimented for its potency in eliciting immunity through humoral and cell mediated immune responses <italic>in vitro</italic>
and <italic>in vivo</italic>
.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s40203-015-0011-4) contains supplementary material, which is available to authorized users.</p>
</sec>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>Ebola</kwd>
<kwd>Computational approach</kwd>
<kwd>RNA polymerase</kwd>
<kwd>Epitope</kwd>
<kwd>Vaccine</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2015</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
<body><sec id="Sec1"><title>Background</title>
<p>EVD, previously designated as Ebola haemorrhagic fever, is a fatal disease in humans and other mammals (monkeys, chimpanzees and gorillas) (Choi and Croyle <xref ref-type="bibr" rid="CR10">2013</xref>
, Leroy et al. <xref ref-type="bibr" rid="CR44">2004</xref>
, Sullivan et al. <xref ref-type="bibr" rid="CR70">2000</xref>
). The fatality rate of EDV is varied from 25 to 90 % with an average of about 50 % (Peters and Peters <xref ref-type="bibr" rid="CR57">1999</xref>
) and it is caused by a virus of the family Filoviridae, genus Ebolavirus. There are five separate Ebola virus species have been identified, four of which are disease causing to humans: Ebola virus (Zaire ebolavirus); Taï Forest virus (Taï Forest ebolavirus, formerly Côte d’Ivoire ebolavirus); Sudan virus (Sudan ebolavirus); and Bundibugyo virus (Bundibugyoebolavirus) (Hoenen et al. <xref ref-type="bibr" rid="CR33">2012</xref>
). The fifth one, Reston virus (Reston ebolavirus), is harmful to nonhuman primates, but not to humans (Elisha and Adegboro <xref ref-type="bibr" rid="CR15">2014</xref>
, Geisbert et al. <xref ref-type="bibr" rid="CR26">2009</xref>
). Among the recognized species of ebolavirus, the notoriously deadly Zaire ebolavirus is responsible for epidemics which have been taken place mainly in African countries including Democratic Republic of Congo, Uganda, Sudan, the Ivory Coast, and Gabon (Baize et al. <xref ref-type="bibr" rid="CR4">2014</xref>
, Chowell et al. <xref ref-type="bibr" rid="CR12">2004</xref>
, Feldmann et al. <xref ref-type="bibr" rid="CR20">2003</xref>
, Frieden et al. <xref ref-type="bibr" rid="CR22">2014</xref>
, Hewlett and Hewlett <xref ref-type="bibr" rid="CR32">2005</xref>
, Kuhn et al. <xref ref-type="bibr" rid="CR41">2010</xref>
, Li and Chen <xref ref-type="bibr" rid="CR45">2014</xref>
, Rouquet et al. <xref ref-type="bibr" rid="CR64">2005</xref>
). This virus is passed on people from wild animals and through human-to-human contact transmits in the human population. Those are infected with this virus bear fearsome symptoms, including high fever, hemoptysis, impaired kidney and liver function and severe internal bleeding (Gatherer <xref ref-type="bibr" rid="CR25">2014</xref>
, Goeijenbier et al. <xref ref-type="bibr" rid="CR28">2014</xref>
, Keiser et al. <xref ref-type="bibr" rid="CR37">2004</xref>
, Peters and Peters <xref ref-type="bibr" rid="CR57">1999</xref>
). In the fall of 2014 the Ebola virus gained widespread attention when in West Africa the largest outbreak has been reported in history.</p>
<p>The EBOV genome is a single-stranded, negative-sense, non-segmented RNA approximately 19 kb long. It codes for seven tandemly arranged viral genes which order is 3′ leader- NP (nucleoprotein) - VP35 (virion protein 35)-VP40- GP (glycoprotein)-VP30-VP24- L (RNA-dependent RNA polymerase)-trailer −5′. Transcription and translation of this viral genome result in the synthesis of seven structural proteins and a single non-structural, secreted glycoprotein (Feldmann et al. <xref ref-type="bibr" rid="CR18">1999</xref>
). Three of the structural proteins are membrane-associated proteins; GP is a type I transmembrane protein, while VP24 and VP40 are placed on the inner surface of the membrane. The remaining four, NP, VP30 (transcription factor), VP35 (polymerase cofactor), and L (RNA-dependent RNA polymerase), are essential to viral genomic RNA to form the ribonucleoprotein complex. These proteins have been shown to be necessary and sufficient for EBOV transcription and replication (Crary et al. <xref ref-type="bibr" rid="CR13">2003</xref>
, Feldmann et al. <xref ref-type="bibr" rid="CR19">2001</xref>
, Mühlberger et al. <xref ref-type="bibr" rid="CR50">1998</xref>
; <xref ref-type="bibr" rid="CR51">1999</xref>
, Takada et al. <xref ref-type="bibr" rid="CR71">1997</xref>
).</p>
<p>To date, information regarding the processing, structure and functions of Ebola virus (EBOV) protein L (EBOL) demonstrates that it is an RNA-dependent RNA polymerase, with the assistance of VP35. It also shows mRNA (guanine-N (7)-)-methyltransferase, mRNA guanylyltransferase and poly (A) synthetase activities which are essential for the replication and transcription of EBOV (Poch et al. <xref ref-type="bibr" rid="CR60">1990</xref>
). The viral mRNA guanylyltransferase serves either as transcriptase or as replicase. The transcriptase synthesizes subgenomic RNAs, assures their capping and polyadenylation. The transcriptase stutters on a specific sequence, leads to a co-transcriptional editing of the glycoprotein (GP) mRNA. In replicase mode, the polymerase replicates the viral genome without recognizing the transcriptional signals. These reports suggest that EBOL is an important cellular component for the transcription and replication of the EBOV genome and, as such, plays a key role in the EBOV life cycle.</p>
<p>Due to the emergence of Ebola virus outbreak, there is an immediate need to determine novel therapeutic targets against this pathogen. The identification of specific epitopes derived from infectious pathogens has significantly advanced the development of epitope-based vaccines (EVs). Bettered understanding of the molecular basis of antigen recognition and HLA binding motifs has resulted in the advancement of rationally designed vaccines depend on algorithms predicting the peptide’s binding to human HLA. In comparison to the conventional vaccines, peptide or epitope based vaccines are easy to develop, chemical stable, more specific, and free of any infectious or oncogenic potential hazard (Holland and Domingo <xref ref-type="bibr" rid="CR34">1998</xref>
, Sette et al. <xref ref-type="bibr" rid="CR68">2002</xref>
). Though EVs have varied advantages, the wet lab based discovery of candidate epitopes is expensive and time consuming. Furthermore, for the final selection of epitopes various immunological requirements are needed to be considered. As a result computational methods, an alternative <italic>in silico</italic>
approaches (Germain <xref ref-type="bibr" rid="CR27">1994</xref>
) have recently been attracting growing interest of the researchers for predicting epitopes with reduced cost and time. The application of bioinformatics in immunology is termed as immunoinformatics. Currently, numerous immunoinformatics tools are available for identifying B and T cell epitopes and human leukocyte antigen (HLA) ligands (Petrovsky and Brusic <xref ref-type="bibr" rid="CR59">2002</xref>
, Poland et al. <xref ref-type="bibr" rid="CR61">2009</xref>
, Sette and Fikes <xref ref-type="bibr" rid="CR67">2003</xref>
) with high sensitivity and specificity. The ‘immunoinformatics’ approach has already proven its potency in the case of human immunodeficiency virus (Wilson et al. <xref ref-type="bibr" rid="CR80">2003</xref>
), multiple sclerosis (Bourdette et al. <xref ref-type="bibr" rid="CR7">2005</xref>
), tuberculosis (Robinson and Amara <xref ref-type="bibr" rid="CR63">2005</xref>
) and malaria (López et al. <xref ref-type="bibr" rid="CR47">2001</xref>
) with desired results. In the present study, we have followed immunoinformatics approaches for designing potential conserved epitope candidate for the utility of vaccine development against the deadly Ebola virus, with an expectation of further wet lab validation.</p>
</sec>
<sec id="Sec2" sec-type="materials|methods"><title>Methods</title>
<sec id="Sec3"><title>Sequence retrieval and conserved region identification</title>
<p>The protein sequences of the RNA-dependent RNA polymerase-L (Volchkov et al. <xref ref-type="bibr" rid="CR75">1999</xref>
) of the EBOV were retrieved from the UniProtKB (Apweiler et al. <xref ref-type="bibr" rid="CR1">2004</xref>
) database in the FASTA format. BioEdit v7.2.3 sequence alignment editor (Apweiler et al. <xref ref-type="bibr" rid="CR1">2004</xref>
) was used for the identification of the conserved region among the sequences through multiple-sequence alignment (MSA) with ClustalW (Hall <xref ref-type="bibr" rid="CR29">1999</xref>
). Finally, Jalview v2 tool (Thompson et al. <xref ref-type="bibr" rid="CR72">1994</xref>
) was used to retrieve the alignment and the CLC Sequence Viewer v7.0.2 (<ext-link ext-link-type="uri" xlink:href="http://www.clcbio.com">http://www.clcbio.com</ext-link>
) was used for analysis of the divergence among the different strains of the EBOV.</p>
</sec>
<sec id="Sec4"><title>Antigenicity determination of the conserved peptides</title>
<p>VaxiJen v2. 0, a Web-based server (Waterhouse et al. <xref ref-type="bibr" rid="CR79">2009</xref>
, Doytchinova and Flower <xref ref-type="bibr" rid="CR14">2007</xref>
) was used for the determination of the antigenicity of the conserved sequences. Herein, we used the default parameters for the prediction, with a threshold value of 0.4.</p>
</sec>
<sec id="Sec5"><title>T-cell epitope prediction</title>
<p>For this study, two online servers were used. Firstly, the NetCTL v1.2 server (Larsen et al. <xref ref-type="bibr" rid="CR42">2007</xref>
) was used for predicting potential cytotoxic T lymphocyte (CTL) epitopes from the conserved peptides. Here for predicting the epitopes, we used a combined algorithm including major histocompatibility complex class I (MHC-I) binding, transporter of antigenic peptide (TAP) transport efficiency, and proteasomal C terminal cleavage prediction. Depending on the score, the best candidates were picked for further investigation. The epitope prediction was confined to 12 MHC-I supertypes. MHC-I binding and proteasomal cleavage were carried out through artificial neural networks and the weight matrix was used to estimate the TAP transport efficiency. The threshold value for epitope identification was set at 0.5 for maintaining sensitivity and specificity of 0.89 and 0.94, respectively during the analysis. This would support to assess the findings more decisively by developing more epitopes. Finally, for confirming the prediction with default parameters, CTLPred (Bhasin and Raghava <xref ref-type="bibr" rid="CR6">2004</xref>
) was employed additionally.</p>
</sec>
<sec id="Sec6"><title>MHC-I and MHC-II restriction analysis</title>
<p>Furthermore, from the Immune Epitope Database and Analysis Resource (IEDB-AR), T Cell Epitope Prediction Tools was implied for the identification of MHC-I (Hoof et al. <xref ref-type="bibr" rid="CR35">2009</xref>
, Nielsen et al. <xref ref-type="bibr" rid="CR53">2007</xref>
) and MHC-II (Wang et al. <xref ref-type="bibr" rid="CR76">2008</xref>
; <xref ref-type="bibr" rid="CR77">2010</xref>
) binding of the peptide. In order to calculate the half-maximal inhibitory concentration (IC<sub>50</sub>
) values required for peptide binding to MHC-I molecules, Stabilized Matrix Method (Peters and Sette <xref ref-type="bibr" rid="CR58">2005</xref>
) was applied with a preset 9.0-mer epitope.</p>
<p>In case of MHC-II binding analysis, the IEDB-recommended method was used for the specific HLA-DQ, HLA-DP, and HLA-DR loci. Herein, specific peptides were used to predict the MHC-II interaction on the basis of MHC-I analysis and antigenic conservancy.</p>
</sec>
<sec id="Sec7"><title>Prediction of population coverage</title>
<p>Population coverage for epitope was assessed by the IEDB population coverage calculation tool (Bui et al. <xref ref-type="bibr" rid="CR8">2006</xref>
). Here we used the allelic frequency of the interacting HLA alleles for the prediction of the population coverage for the corresponding epitope.</p>
</sec>
<sec id="Sec8"><title>B-cell epitope prediction</title>
<p>Linear B cell epitopes are of different lengths of peptides from 2 to 85 in comparison to that of T cell epitopes. B-cell epitope produces immune response when it interacts with B lymphocytes. It then initiates the differentiation of B lymphocytes into plasma and memory cells (Nair et al. <xref ref-type="bibr" rid="CR52">2002</xref>
). There are a number of Web-based tools are available for the prediction of B-cell epitope which are hosted by IEDB-AR. For the B-cell epitope prediction with high accuracy, multiple tools, including the Emini surface accessibility prediction (Emini et al. <xref ref-type="bibr" rid="CR16">1985</xref>
), Kolaskar and Tongaonkar antigenicity scale (Kolaskar and Tongaonkar <xref ref-type="bibr" rid="CR40">1990</xref>
), Parker hydrophilicity prediction, (Parker et al. <xref ref-type="bibr" rid="CR56">1986</xref>
) and finally the Chou and Fasman beta turn prediction tool (Chou and Fasman <xref ref-type="bibr" rid="CR11">1979</xref>
) were employed, because the antigenic parts of a protein belong to the beta turn regions (Rini et al. <xref ref-type="bibr" rid="CR62">1992</xref>
).</p>
</sec>
<sec id="Sec9"><title>Homology modeling and protein variability determination of the conserved region</title>
<p>The structure of the conserved region was constructed by homology modelling using the MODELLER v9 (Šali et al. <xref ref-type="bibr" rid="CR66">1995</xref>
). MODELLER is a program that implements an automated approach to comparative protein structure modelling by satisfying spatial restraints (Fiser et al. <xref ref-type="bibr" rid="CR21">2000</xref>
, Sali and Blundell <xref ref-type="bibr" rid="CR65">1993</xref>
). Finally, the evaluation of the predicted model was verified by using two software tools, PROCHECK (Arnold et al. <xref ref-type="bibr" rid="CR2">2006</xref>
, Laskowski et al. <xref ref-type="bibr" rid="CR43">1996</xref>
) and QMEAN (Benkert et al. <xref ref-type="bibr" rid="CR5">2011</xref>
). For predicting the disorder among the amino acid sequences, DISOPRED v3 (Ward et al. <xref ref-type="bibr" rid="CR78">2004</xref>
) server was used. In order to calculate the protein variability index the Protein variability server was implied where Wu-Kabat variability coefficient (Garcia-Boronat et al. <xref ref-type="bibr" rid="CR24">2008</xref>
) has been used.</p>
</sec>
<sec id="Sec10"><title>Allergenicity and epitope conservancy analysis</title>
<p>The web-based AllerHunter server (Muh et al. <xref ref-type="bibr" rid="CR49">2009</xref>
) was used to predict the allergenicity of our proposed epitope for vaccine development. This server predicts allergenicity through a combinational prediction, by using both integration of the Food and Agriculture Organization (FAO)/World Health Organization (WHO) allergenicity evaluation scheme and support vector machines (SVM)-pairwise sequence similarity. AllerHunter predicts allergens as well as nonallergens with high specificity. This makes AllerHunter is a very useful program for allergen cross-reactivity prediction (Liao and Noble <xref ref-type="bibr" rid="CR46">2003</xref>
).</p>
<p>Epitope conservancy of the candidate epitopes was examined using a Web-based epitope conservancy tool available in IEDB analysis resource (Bui et al. <xref ref-type="bibr" rid="CR9">2007</xref>
). The conservancy level of each potential epitope was calculated by looking for identities in all RNA-dependent RNA polymerase-L protein sequences of different strains retrieved from database.</p>
</sec>
</sec>
<sec id="Sec11" sec-type="results"><title>Results</title>
<sec id="Sec12"><title>Analysis of the retrieved sequences with divergence and antigenicity</title>
<p>A total of 52 RNA-dependent RNA polymerase-L protein molecules from different variants of the EBOV were retrieved from the UniProt database. The MSA of the RNA-dependent polymerase-L proteins was retrieved from BioEdit tool through ClustalW with 1000 bootstrap replicates (Additional file <xref rid="MOESM1" ref-type="media">1</xref>
: Figure S1). CLC Sequence Viewer was used to construct phylograms from the MSA obtained from BioEdit, in order to analyze the divergence among the retrieved sequences. Phylogram of RNA-dependent RNA polymerase-L is depicted in Fig. <xref rid="Fig1" ref-type="fig">1</xref>
. Finally, the highly conserved region from the MSA was retrieved for the further analysis. The selected conserved region is depicted in the Fig. <xref rid="Fig2" ref-type="fig">2</xref>
, from the MSA number 586 to 660. Then the VaxiJen v2.0 server calculate the antigenicity of the conserved sequences with a score 0.4888.<fig id="Fig1"><label>Fig. 1</label>
<caption><p>Phylogenetic tree showing the evolutionary divergence among the different RNA-dependent RNA polymerase-L proteins of the EBOV. Notes: Here, cladogram view is shown with appropriate distance among the different strains. The red dotted view indicates the node of the tree</p>
</caption>
<graphic xlink:href="40203_2015_11_Fig1_HTML" id="MO1"></graphic>
</fig>
<fig id="Fig2"><label>Fig. 2</label>
<caption><p>MSA of the conserved region of RNA-dependent RNA polymerase-L. Only the conserved sequences containing the proposed epitope sequence are shown here. Notes: Clustalx color is used here. Different colors indicate different amino acid residues. The yellow bas at the bottom indicates the conservation of the amino acid residues</p>
</caption>
<graphic xlink:href="40203_2015_11_Fig2_HTML" id="MO2"></graphic>
</fig>
</p>
</sec>
<sec id="Sec13"><title>Identification of T-cell epitope and MHC interaction analysis</title>
<p>T-cell epitopes were selected firstly by using the NetCTL v1.2 server where the epitope prediction was confined to 12 MHC-I supertypes. Based on the combined score, the top five epitopes (Table <xref rid="Tab1" ref-type="table">1</xref>
) were listed for further analysis. T-cell epitopes were again predicted by the CTLPred server (Table <xref rid="Tab2" ref-type="table">2</xref>
). Here a combined approach of artificial neural networks and support vector machines was applied. Depending on the two analyses, the most common epitope—containing peptides, identified by both servers, was selected. The selected epitope was then used for the MHC-binding analysis.<table-wrap id="Tab1"><label>Table 1</label>
<caption><p>Prediction of the T-cell epitope by NetCTL server on the basis of combined score</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr valign="top"><th>Epitope</th>
<th>Start position</th>
<th>Combined score</th>
</tr>
</thead>
<tbody><tr valign="top"><td>FIEYCNHCY</td>
<td>64</td>
<td>2.4978</td>
</tr>
<tr valign="top"><td>FRYEFTAPF</td>
<td>56</td>
<td>2.0697</td>
</tr>
<tr valign="top"><td>RYEFTAPFI</td>
<td>57</td>
<td>1.6395</td>
</tr>
<tr valign="top"><td>ESLLHQASW</td>
<td>19</td>
<td>1.2675</td>
</tr>
<tr valign="top"><td>SFVTDLEKY</td>
<td>44</td>
<td>1.1582</td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="Tab2"><label>Table 2</label>
<caption><p>Prediction of the T-cell epitope by CTLPred server</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr valign="top"><th>Epitope</th>
<th>Start position</th>
<th>Score(ANN/SVM)</th>
</tr>
</thead>
<tbody><tr valign="top"><td>KYNLAFRYE</td>
<td>51</td>
<td>0.87/0.51591091</td>
</tr>
<tr valign="top"><td>RYEFTAPFI</td>
<td>57</td>
<td>0.45/0.69332887</td>
</tr>
<tr valign="top"><td>FRYEFTAPF</td>
<td>56</td>
<td>0.84/0.29033079</td>
</tr>
<tr valign="top"><td>KAFPSNMMV</td>
<td>3</td>
<td>0.64/0.46418851</td>
</tr>
<tr valign="top"><td>LAKAFPSNM</td>
<td>1</td>
<td>0.46/0.61842782</td>
</tr>
</tbody>
</table>
</table-wrap>
</p>
<p>MHC-I-binding prediction, which was run through the Stabilized Matrix Method, predicted a wide range of <italic>MHC-I</italic>
allele interactions for the proposed T-cell epitopes. The <italic>MHC-I</italic>
alleles for which the epitope showed higher affinity (IC<sub>50</sub>
< 250 nM) are listed in Table <xref rid="Tab3" ref-type="table">3</xref>
. The output of the <italic>MHC-II</italic>
interaction analysis is also shown in Table <xref rid="Tab3" ref-type="table">3</xref>
.<table-wrap id="Tab3"><label>Table 3</label>
<caption><p>MHC-I and MHC-II interaction of the proposed sequence by IEDB analysis resource</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr valign="top"><th>Epitope</th>
<th>MHC I interaction</th>
<th>Epitope</th>
<th>MHC II interaction</th>
</tr>
</thead>
<tbody><tr valign="top"><td>FRYEFTAPF</td>
<td>HLA-C*03:02,HLA-C*07:02, HLA-C*12:03,HLA-C*14:02, HLA-C*16:01,HLA-C*06:02, HLA-C*07:01,HLA-C*12:02, HLA-B*27:05, HLA-B*39:01</td>
<td>NLAFRYEFTAPFIEY</td>
<td>HLA-DRB3*01:01, HLA-DQA1*04:01, HLA-DRB3*02:02, HLA-DRB1*03:01, HLA-DRB1*04:01, HLA-DRB1*04:05, HLA-DRB5*01:01, HLA-DPA1*02:01, HLA-DPA1*01:03, HLA-DQA1*03:01, HLA-DRB1*07:01, HLA-DRB1*08:02, HLA-DPA1*01, HLA-DRB1*11:01, HLA-DPA1*02:01, HLA-DQA1*05:01, HLA-DPA1*02:01, HLA-DRB1*09:01, HLA-DQA1*01:01, HLA-DPA1*03:01, HLA-DRB1*15:01, HLA-DRB1*13:02, HLA-DRB1*12:01, HLA-DRB4*01:01, HLA-DQA1*05:01, HLA-DQA1*01:02</td>
</tr>
</tbody>
</table>
</table-wrap>
</p>
</sec>
<sec id="Sec14"><title>Analysis of the population coverage</title>
<p>IEDB population coverage tool analyzed the Population coverage of the proposed epitope. The combined MHC-I and MHC-II class were assessed against the whole world population with the selected <italic>MHC-I</italic>
and <italic>MHC-II</italic>
interacted alleles (Fig. <xref rid="Fig3" ref-type="fig">3</xref>
).<fig id="Fig3"><label>Fig. 3</label>
<caption><p>Population coverage based on MHC–I and MHC-II restriction data. The whole world populations are assessed for the proposed epitope. Notes: In the graphs, the line (−o-) represents the cumulative percentage of population coverage of the epitopes; the bars represent the population coverage for each epitope</p>
</caption>
<graphic xlink:href="40203_2015_11_Fig3_HTML" id="MO3"></graphic>
</fig>
</p>
</sec>
<sec id="Sec15"><title>Prediction B-cell epitope</title>
<p>Here, for predicting potential B-cell epitopes, we used amino acid–based methods. According to this procedure different analysis methods were applied for the identification of a continuous B cell epitope.</p>
<p>The Kolaskar and Tongaonkar antigenicity scale was used for assessing the antigenic property of the peptides. The average antigenic propensity of the protein was 1.014, with a maximum of 1.033 and a minimum of 1.002. For the protein the antigenic determination threshold value was 1.0, where all values equal or greater than 1.0 were potential antigenic determinants. The antigenic plot is depicted in the Fig. <xref rid="Fig4" ref-type="fig">4</xref>
.<fig id="Fig4"><label>Fig. 4</label>
<caption><p>Kolaskar and Tongaonkar antigenicity prediction of the proposed epitope. Notes: The X- and Y-axes represent the sequence position and antigenic propensity score, respectively. The threshold value is 1.0. The regions above the threshold are antigenic, shown in yellow</p>
</caption>
<graphic xlink:href="40203_2015_11_Fig4_HTML" id="MO4"></graphic>
</fig>
</p>
<p>To be a potent B cell epitope, it must be surface accessible. Hence, Emini surface accessibility prediction was employed, with a maximum propensity score of 1.297 at threshold 1.0 (Fig. <xref rid="Fig5" ref-type="fig">5</xref>
). To strengthen our support for the prediction of the epitope to elicit B cell response the Parker hydrophilicity and the Chou and Fasman beta turn prediction were employed. Those are described in the Figs. <xref rid="Fig6" ref-type="fig">6</xref>
and <xref rid="Fig7" ref-type="fig">7</xref>
.<fig id="Fig5"><label>Fig. 5</label>
<caption><p>Emini surface accessibility prediction of the proposed epitope, with a minimum propensity score of 0.566 and maximum score of 1.297. Notes: The X- and Y-axes represent the sequence position and surface probability, respectively. The threshold value is 1.0. The regions above the threshold are antigenic, shown in yellow</p>
</caption>
<graphic xlink:href="40203_2015_11_Fig5_HTML" id="MO5"></graphic>
</fig>
<fig id="Fig6"><label>Fig. 6</label>
<caption><p>Parker hydrophilicity prediction of the epitope, with a minimum propensity score of −0.443 and maximum score of 1.471. Notes: The X- and Y-axes represent the sequence position and antigenic propensity score, respectively. The threshold value is 1.0. The regions above the threshold are antigenic, shown in yellow</p>
</caption>
<graphic xlink:href="40203_2015_11_Fig6_HTML" id="MO6"></graphic>
</fig>
<fig id="Fig7"><label>Fig. 7</label>
<caption><p>Chou and Fasman beta turn prediction of the epitope,with a minimum propensity score of 0.878 and maximum score of 0.939. Notes: The X- and Y-axes represent the sequence position and antigenic propensity score, respectively. The threshold value is 1.0. The regions above the threshold are antigenic, shown in yellow</p>
</caption>
<graphic xlink:href="40203_2015_11_Fig7_HTML" id="MO7"></graphic>
</fig>
</p>
</sec>
<sec id="Sec16"><title>Structure analysis and protein variability determination</title>
<p>Homology model of the conserved region was obtained by the MODELLER software, which is shown in Fig. <xref rid="Fig8" ref-type="fig">8a</xref>
and <xref rid="Fig8" ref-type="fig">b</xref>
. PROCHECK server validated the stereochemical quality of the model through Ramachandran Plot (Fig. <xref rid="Fig8" ref-type="fig">8c</xref>
), andQMEAN server also assessed the tertiary structure, with a Qmean6 score of 0.327. DISOPRED v3 server predicted the disorder of the conserved peptide in order to get insight about the disorder among the conserved sequences, which is depicted in Fig. <xref rid="Fig9" ref-type="fig">9</xref>
. Protein variability server predicted the variability of the conserved region of the RNA-dependent RNA polymerase-L (Fig. <xref rid="Fig10" ref-type="fig">10</xref>
) to ensure that the proposed epitope is within the invariable region.<fig id="Fig8"><label>Fig. 8</label>
<caption><p>Three-dimensional model of the RNA-dependent RNA polymerase-L with the proposed epitope and validation. Notes: Two different view of the modeled protein (Blue spherical) with the predicted epitope (Red spherical). <bold>a</bold>
Top view. <bold>b</bold>
Side view. The outerside location of the epitope indicates its surface accessibility. <bold>c</bold>
Ramachandran plot of the predicted model shows that most of the residues are in the allowed region of the plot, proving the validity of the model</p>
</caption>
<graphic xlink:href="40203_2015_11_Fig8_HTML" id="MO8"></graphic>
</fig>
<fig id="Fig9"><label>Fig. 9</label>
<caption><p>Disorder prediction of the conserved antigenic amino acid sequences. Here, our proposed epitope lies outside (56–64) of the disordered region to secure its potentiality as an effective epitope. Notes: Amino acids in the input sequence are considered disordered when the blue line is above the gray dashed line, that is, when the confidence score is 0.5. The orange line shows the confidence score of the disordered protein-binding residue predictions</p>
</caption>
<graphic xlink:href="40203_2015_11_Fig9_HTML" id="MO9"></graphic>
</fig>
<fig id="Fig10"><label>Fig. 10</label>
<caption><p>Protein variability index of the conserved peptides of all the sequences. The prediction suggests that our proposed epitope “FRYEFTAPF” falls in the invariable region (Blue line). Notes: The conservancy threshold was 1.0 in this analysis. The X-axis indicates the amino acid positions in the sequences and the Y-axis indicates the Shannon variability score</p>
</caption>
<graphic xlink:href="40203_2015_11_Fig10_HTML" id="MO10"></graphic>
</fig>
</p>
</sec>
<sec id="Sec17"><title>Epitope conservancy and allergenicity analysis</title>
<p>Conservation analyses of the proposed epitopes were analyzed by the IEDB conservancy analysis tool that is shown in Table <xref rid="Tab4" ref-type="table">4</xref>
. AllerHunter server predicted the allergenicity of the queried epitope with a score was 0.03 (sensitivity =94.4. %, specificity =70.3 %).<table-wrap id="Tab4"><label>Table 4</label>
<caption><p>Epitope conservancy analysis</p>
</caption>
<table frame="hsides" rules="groups"><thead><tr valign="top"><th>Peptide sequence</th>
<th>Peptide length</th>
<th>Percentage of protein sequence match</th>
<th>Maximum identity</th>
</tr>
</thead>
<tbody><tr valign="top"><td>FRYEFTAPF</td>
<td>9</td>
<td>100 % (52/52)</td>
<td>100 %</td>
</tr>
<tr valign="top"><td>NLAFRYEFTAPFIEY</td>
<td>15</td>
<td>100 % (52/52)</td>
<td>100 %</td>
</tr>
</tbody>
</table>
</table-wrap>
</p>
</sec>
</sec>
<sec id="Sec18" sec-type="discussion"><title>Discussion</title>
<p>Our world is the habitation of more than seven billion people now. With the upgrade of medical science, new viruses along with their causing diseases are also emerging. Ebola virus is such kinds of virus with a deadly outrage of their endemic nature especially in Africa in recent time (Evans and Popova <xref ref-type="bibr" rid="CR17">2015</xref>
). Till now there is no potential treatment for this virus to combat its deadly effects.</p>
<p>Recent time, the immunoinformatics approach give us some sort of hope for the design of an effective therapeutics, like vaccine, in association with the advancement of sequence based technology. Similar approaches have been used successfully for identifying vaccine candidates in several pathogens viz. human corona virus (Oany et al. <xref ref-type="bibr" rid="CR54">2008</xref>
), Saint Louis encephalitis virus (Hasan et al. <xref ref-type="bibr" rid="CR30">2013</xref>
), Crimean–Congo hemorrhagic fever virus (Oany et al. <xref ref-type="bibr" rid="CR55">2015</xref>
), Chikungunya virus (Hasan et al. <xref ref-type="bibr" rid="CR31">2015</xref>
) and some others. The <italic>in vitro</italic>
validation of this type of work has also been proven in recent time (Khan et al. <xref ref-type="bibr" rid="CR38">2014</xref>
).</p>
<p>Though epitope-based vaccine designing has become a familiar approach, in the case of EBOV no significant work yet has been done. EBOV is an RNA virus which has genetic blueprints made of RNA instead of DNA. Creating vaccines is particularly difficult for RNA viruses as they can quickly mutate their different exposed proteins (Twiddy et al. <xref ref-type="bibr" rid="CR74">2003</xref>
). Therefore the most potential way to create stable antiviral therapies against RNA viruses including EBOV is to target the transcription or replication machinery. Scientists revealed that RNA-dependent RNA polymerase-L (EBOL) is an important cellular component for the transcription and replication of the EBOV genome. When an EBOV infects a cell, its RNA genetic blueprint enters the cell along with RNA-dependent RNA polymerase-L. This polymerase normally “read” the RNA genetic blueprint in order to synthesize mRNA, which then leads to the formation of viral proteins as well as viral replication and more viral particles are produced. For these two vital involvements at the gateway, this protein was targeted to design most potential epitopes using <italic>in silico</italic>
computational approaches.</p>
<p>In the current study, firstly all the available sequences of RNA-dependent RNA polymerase-Lwere retrieved from database. Then antigenicity of the conserved peptides, generated by multiple sequence alignment was predicted by VaxiJen, which suggested their ability to elicit potential immune response. Sequence based bioinformatics approaches were applied to predict both B cell and T cell epitopes for conferring immunity in different ways. Though at present, most of the vaccines are based on B cell immunity; vaccines based on T cell epitope have been encouraged recently. It is because, with time humoral response from memory B cells can be overcome easily by antigenic drift, while cell mediated immunity often provides long lasting immunity (Bacchetta et al. <xref ref-type="bibr" rid="CR3">2005</xref>
, Igietseme et al. <xref ref-type="bibr" rid="CR36">2004</xref>
). Cytotoxic CD8<sup>+</sup>
T lymphocytes (CTL) inhibit the spread of infectious agents by recognizing and killing infected cells or secreting specific antiviral cytokines (Garcia et al. <xref ref-type="bibr" rid="CR23">1999</xref>
, Shrestha and Diamond <xref ref-type="bibr" rid="CR69">2004</xref>
). Thus, vaccination based on T cell epitope is a unique approach to obtain strong immune response against infectious agents, such as, viruses (Klein et al. <xref ref-type="bibr" rid="CR39">2005</xref>
).</p>
<p>Both NetCTL and CTLPred server were used to find epitopes for the activation of T-cell immunity with potential antigenicity. By examining the output it was predicted that FRYEFTAPF would be the best epitope candidate and was further subjected for binding proficiency analysis.</p>
<p>Length is an important factor to consider for peptide antigen binding with MHC or TCR or both. T cell epitopes presented by MHC class I molecules are generally peptides between 8 and 11 amino acids in length. We therefore set peptide lengths at 9 before making software based MHC class I T cell epitope identification using immune epitope database (IEDB). Analysis revealed that the core epitope “FRYEFTAPF” would interact with ten different MHC class I alleles. On the other hand, the complete peptide “NLAFRYEFTAPFIEY” interacts with the highest numbers of MHC class II alleles (as many as 26 alleles).</p>
<p>Along with the T-cell epitope, in our study, attention was also given to the B-cell epitope, which can induce both primary and secondary humoral immunity (Trainor et al. <xref ref-type="bibr" rid="CR73">2007</xref>
). Multiple prediction methods were applied to determine the B-cell epitope considering several criteria of antigenicity, hydrophilicity, surface accessibility, and beta-turn. Our proposed epitope has met all the criteria of the above B-cell prediction methods.</p>
<p>The three-dimensional model of the conserved protein ensured the exact location of the epitope outside of the protein (Fig. <xref rid="Fig8" ref-type="fig">8a</xref>
and <xref rid="Fig8" ref-type="fig">b</xref>
) surface and the model validity was assessed by Ramachandran Plot (Fig. <xref rid="Fig8" ref-type="fig">8c</xref>
), whereby 87.8 % amino acid residues were found within the favored region. The epitope was also treated as suitable candidate for vaccine through tenabled its position in the conserved sequence, by the Discopred and protein variability server (Figs. <xref rid="Fig9" ref-type="fig">9</xref>
and <xref rid="Fig10" ref-type="fig">10</xref>
).</p>
<p>Conservancy is the most important criterion of an epitope to consider it for vaccine development. Conservancy analysis of our proposed epitope showed 100 % conservancy among all the available sequences. Another important feature of the peptide vaccine is its allergenicity (McKeever et al. <xref ref-type="bibr" rid="CR48">2004</xref>
). <italic>In silico</italic>
analysis revealed that the proposed epitope is nonallergenic in nature.</p>
<p>Wide range population coverage must be needed for a potential vaccine aspirant. At this point, our proposed epitope covers a remarkable population of 99.87 % for both types of <italic>MHC allele</italic>
throughout the world population. That makes the epitope as a supreme candidate for vaccine consideration.</p>
<p>Finally, from the above <italic>in silico</italic>
analysis, we are really optimistic that our proposed epitope would trigger an immune response <italic>in vitro and in vivo</italic>
.</p>
</sec>
<sec id="Sec19" sec-type="conclusions"><title>Conclusion</title>
<p>A number of approaches exist for new vaccine development, such as recombinant vaccines, sub-unit protein and DNA vaccines, auxotrophic organisms to deliver genes and so on. Current study is an attempt to identify potential epitope targets against EBOV using different computational tools. It is quite obvious that in order to minimize the deadly effects of EBOV, highly potential drugs are immediately required and these <italic>in silico</italic>
approaches will reduce the wet lab efforts with higher probability of success. Therefore, it is concluded that the identified epitope may be exploited further for developing epitope-based vaccine against EBOV. Nevertheless, the initial hints we obtained will help to prioritize potential therapeutics for EBOV.</p>
</sec>
</body>
<back><app-group><app id="App1"><sec id="Sec20"><title>Additional file</title>
<p><media position="anchor" xlink:href="40203_2015_11_MOESM1_ESM.png" id="MOESM1"><label>Additional file 1: Figure S1.</label>
<caption><p>MSA of the RNA-dependent polymerase-L proteins of the different EBOV. (PNG 1540 kb)</p>
</caption>
</media>
</p>
</sec>
</app>
</app-group>
<fn-group><fn><p><bold>Competing interests</bold>
</p>
<p>The authors declare that they have no competing interests.</p>
</fn>
<fn><p><bold>Authors’ contributions</bold>
</p>
<p>ARO has made substantial contributions to conception and design, acquisition of data, analysis and interpretation of data. TS and ASC carried out the molecular genetic studies, participated in the sequence alignment and drafted the manuscript. TPJ worked for computational analysis. MAH conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.</p>
</fn>
</fn-group>
<ref-list id="Bib1"><title>References</title>
<ref id="CR1"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Apweiler</surname>
<given-names>R</given-names>
</name>
<name><surname>Bairoch</surname>
<given-names>A</given-names>
</name>
<name><surname>Wu</surname>
<given-names>CH</given-names>
</name>
<etal></etal>
</person-group>
<article-title>UniProt: the universal protein knowledgebase</article-title>
<source>Nucleic Acids Res</source>
<year>2004</year>
<volume>32</volume>
<issue>1</issue>
<fpage>115</fpage>
<lpage>119</lpage>
<pub-id pub-id-type="doi">10.1093/nar/gkh131</pub-id>
<pub-id pub-id-type="pmid">14704348</pub-id>
</element-citation>
</ref>
<ref id="CR2"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Arnold</surname>
<given-names>K</given-names>
</name>
<name><surname>Bordoli</surname>
<given-names>L</given-names>
</name>
<name><surname>Kopp</surname>
<given-names>J</given-names>
</name>
<etal></etal>
</person-group>
<article-title>The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling</article-title>
<source>Bioinformatics</source>
<year>2006</year>
<volume>22</volume>
<issue>2</issue>
<fpage>195</fpage>
<lpage>201</lpage>
<pub-id pub-id-type="doi">10.1093/bioinformatics/bti770</pub-id>
<pub-id pub-id-type="pmid">16301204</pub-id>
</element-citation>
</ref>
<ref id="CR3"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bacchetta</surname>
<given-names>R</given-names>
</name>
<name><surname>Gregori</surname>
<given-names>S</given-names>
</name>
<name><surname>Roncarolo</surname>
<given-names>M-G</given-names>
</name>
</person-group>
<article-title>CD4+ regulatory T cells: mechanisms of induction and effector function</article-title>
<source>Autoimmun Rev</source>
<year>2005</year>
<volume>4</volume>
<issue>8</issue>
<fpage>491</fpage>
<lpage>496</lpage>
<pub-id pub-id-type="doi">10.1016/j.autrev.2005.04.005</pub-id>
<pub-id pub-id-type="pmid">16214084</pub-id>
</element-citation>
</ref>
<ref id="CR4"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Baize</surname>
<given-names>S</given-names>
</name>
<name><surname>Pannetier</surname>
<given-names>D</given-names>
</name>
<name><surname>Oestereich</surname>
<given-names>L</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Emergence of Zaire Ebola virus disease in Guinea</article-title>
<source>N Engl J Med</source>
<year>2014</year>
<volume>371</volume>
<issue>15</issue>
<fpage>1418</fpage>
<lpage>1425</lpage>
<pub-id pub-id-type="doi">10.1056/NEJMoa1404505</pub-id>
<pub-id pub-id-type="pmid">24738640</pub-id>
</element-citation>
</ref>
<ref id="CR5"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Benkert</surname>
<given-names>P</given-names>
</name>
<name><surname>Biasini</surname>
<given-names>M</given-names>
</name>
<name><surname>Schwede</surname>
<given-names>T</given-names>
</name>
</person-group>
<article-title>Toward the estimation of the absolute quality of individual protein structure models</article-title>
<source>Bioinformatics</source>
<year>2011</year>
<volume>27</volume>
<issue>3</issue>
<fpage>343</fpage>
<lpage>350</lpage>
<pub-id pub-id-type="doi">10.1093/bioinformatics/btq662</pub-id>
<pub-id pub-id-type="pmid">21134891</pub-id>
</element-citation>
</ref>
<ref id="CR6"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bhasin</surname>
<given-names>M</given-names>
</name>
<name><surname>Raghava</surname>
<given-names>G</given-names>
</name>
</person-group>
<article-title>Prediction of CTL epitopes using QM, SVM and ANN techniques</article-title>
<source>Vaccine</source>
<year>2004</year>
<volume>22</volume>
<issue>23</issue>
<fpage>3195</fpage>
<lpage>3204</lpage>
<pub-id pub-id-type="doi">10.1016/j.vaccine.2004.02.005</pub-id>
<pub-id pub-id-type="pmid">15297074</pub-id>
</element-citation>
</ref>
<ref id="CR7"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bourdette</surname>
<given-names>DN</given-names>
</name>
<name><surname>Edmonds</surname>
<given-names>E</given-names>
</name>
<name><surname>Smith</surname>
<given-names>C</given-names>
</name>
<etal></etal>
</person-group>
<article-title>A highly immunogenic trivalent T cell receptor peptide vaccine for multiple sclerosis</article-title>
<source>Mult Scler</source>
<year>2005</year>
<volume>11</volume>
<issue>5</issue>
<fpage>552</fpage>
<lpage>561</lpage>
<pub-id pub-id-type="doi">10.1191/1352458505ms1225oa</pub-id>
<pub-id pub-id-type="pmid">16193893</pub-id>
</element-citation>
</ref>
<ref id="CR8"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bui</surname>
<given-names>HH</given-names>
</name>
<name><surname>Sidney</surname>
<given-names>J</given-names>
</name>
<name><surname>Dinh</surname>
<given-names>K</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Predicting population coverage of T-cell epitope-based diagnostics and vaccines</article-title>
<source>BMC Bioinformatics</source>
<year>2006</year>
<volume>17</volume>
<issue>7</issue>
<fpage>153</fpage>
<pub-id pub-id-type="doi">10.1186/1471-2105-7-153</pub-id>
<pub-id pub-id-type="pmid">16545123</pub-id>
</element-citation>
</ref>
<ref id="CR9"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bui</surname>
<given-names>HH</given-names>
</name>
<name><surname>Sidney</surname>
<given-names>J</given-names>
</name>
<name><surname>Li</surname>
<given-names>W</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Development of an epitope conservancy analysis tool to facilitate the design of epitope-based diagnostics and vaccines</article-title>
<source>BMC Bioinformatics</source>
<year>2007</year>
<volume>8</volume>
<issue>1</issue>
<fpage>361</fpage>
<pub-id pub-id-type="doi">10.1186/1471-2105-8-361</pub-id>
<pub-id pub-id-type="pmid">17897458</pub-id>
</element-citation>
</ref>
<ref id="CR10"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Choi</surname>
<given-names>JH</given-names>
</name>
<name><surname>Croyle</surname>
<given-names>MA</given-names>
</name>
</person-group>
<article-title>Emerging targets and novel approaches to Ebola virus prophylaxis and treatment</article-title>
<source>BioDrugs</source>
<year>2013</year>
<volume>27</volume>
<issue>6</issue>
<fpage>565</fpage>
<lpage>583</lpage>
<pub-id pub-id-type="doi">10.1007/s40259-013-0046-1</pub-id>
<pub-id pub-id-type="pmid">23813435</pub-id>
</element-citation>
</ref>
<ref id="CR11"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chou</surname>
<given-names>PY</given-names>
</name>
<name><surname>Fasman</surname>
<given-names>GD</given-names>
</name>
</person-group>
<article-title>Empirical predictions of protein conformation</article-title>
<source>Annu Rev Biochem</source>
<year>1979</year>
<volume>47</volume>
<fpage>251</fpage>
<lpage>276</lpage>
<pub-id pub-id-type="doi">10.1146/annurev.bi.47.070178.001343</pub-id>
<pub-id pub-id-type="pmid">354496</pub-id>
</element-citation>
</ref>
<ref id="CR12"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chowell</surname>
<given-names>G</given-names>
</name>
<name><surname>Hengartner</surname>
<given-names>NW</given-names>
</name>
<name><surname>Castillo-Chavez</surname>
<given-names>C</given-names>
</name>
<etal></etal>
</person-group>
<article-title>The basic reproductive number of Ebola and the effects of public health measures: the cases of Congo and Uganda</article-title>
<source>J Theor Biol</source>
<year>2004</year>
<volume>229</volume>
<issue>1</issue>
<fpage>119</fpage>
<lpage>126</lpage>
<pub-id pub-id-type="doi">10.1016/j.jtbi.2004.03.006</pub-id>
<pub-id pub-id-type="pmid">15178190</pub-id>
</element-citation>
</ref>
<ref id="CR13"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Crary</surname>
<given-names>S</given-names>
</name>
<name><surname>Towner</surname>
<given-names>J</given-names>
</name>
<name><surname>Honig</surname>
<given-names>J</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Analysis of the role of predicted RNA secondary structures in Ebola virus replication</article-title>
<source>Virology</source>
<year>2003</year>
<volume>306</volume>
<fpage>210</fpage>
<lpage>218</lpage>
<pub-id pub-id-type="doi">10.1016/S0042-6822(02)00014-4</pub-id>
<pub-id pub-id-type="pmid">12642094</pub-id>
</element-citation>
</ref>
<ref id="CR14"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Doytchinova</surname>
<given-names>IA</given-names>
</name>
<name><surname>Flower</surname>
<given-names>DR</given-names>
</name>
</person-group>
<article-title>VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines</article-title>
<source>BMC Bioinformatics</source>
<year>2007</year>
<volume>8</volume>
<issue>1</issue>
<fpage>4</fpage>
<pub-id pub-id-type="doi">10.1186/1471-2105-8-4</pub-id>
<pub-id pub-id-type="pmid">17207271</pub-id>
</element-citation>
</ref>
<ref id="CR15"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Elisha</surname>
<given-names>A</given-names>
</name>
<name><surname>Adegboro</surname>
<given-names>B</given-names>
</name>
</person-group>
<article-title>Ebola virus diseases</article-title>
<source>Afr J Clin Exp Microbiol</source>
<year>2014</year>
<volume>15</volume>
<issue>3</issue>
<fpage>117</fpage>
<lpage>121</lpage>
<pub-id pub-id-type="doi">10.4314/ajcem.v15i3.1</pub-id>
</element-citation>
</ref>
<ref id="CR16"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Emini</surname>
<given-names>EA</given-names>
</name>
<name><surname>Hughes</surname>
<given-names>JV</given-names>
</name>
<name><surname>Perlow</surname>
<given-names>DS</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide</article-title>
<source>J Virol</source>
<year>1985</year>
<volume>55</volume>
<issue>3</issue>
<fpage>836</fpage>
<lpage>839</lpage>
<pub-id pub-id-type="pmid">2991600</pub-id>
</element-citation>
</ref>
<ref id="CR17"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Evans</surname>
<given-names>DK</given-names>
</name>
<name><surname>Popova</surname>
<given-names>A</given-names>
</name>
</person-group>
<article-title>West African Ebola crisis and orphans</article-title>
<source>Lancet</source>
<year>2015</year>
<volume>385</volume>
<issue>9972</issue>
<fpage>945</fpage>
<lpage>946</lpage>
<pub-id pub-id-type="doi">10.1016/S0140-6736(15)60179-9</pub-id>
<pub-id pub-id-type="pmid">25715992</pub-id>
</element-citation>
</ref>
<ref id="CR18"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Feldmann</surname>
<given-names>H</given-names>
</name>
<name><surname>Volchkov</surname>
<given-names>VE</given-names>
</name>
<name><surname>Volchkova</surname>
<given-names>VA</given-names>
</name>
<etal></etal>
</person-group>
<article-title>The glycoproteins of Marburg and Ebola virus and their potential roles in pathogenesis</article-title>
<source>Arch Virol</source>
<year>1999</year>
<volume>15</volume>
<fpage>159</fpage>
<lpage>169</lpage>
</element-citation>
</ref>
<ref id="CR19"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Feldmann</surname>
<given-names>H</given-names>
</name>
<name><surname>Volchkov</surname>
<given-names>V</given-names>
</name>
<name><surname>Ströher</surname>
<given-names>U</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Biosynthesis and role of filovirus glycoprotein</article-title>
<source>J Gen Virol</source>
<year>2001</year>
<volume>82</volume>
<fpage>2839</fpage>
<lpage>2848</lpage>
<pub-id pub-id-type="pmid">11714958</pub-id>
</element-citation>
</ref>
<ref id="CR20"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Feldmann</surname>
<given-names>H</given-names>
</name>
<name><surname>Jones</surname>
<given-names>S</given-names>
</name>
<name><surname>Klenk</surname>
<given-names>HD</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Ebola virus: from discovery to vaccine</article-title>
<source>Nat Rev Immunol</source>
<year>2003</year>
<volume>3</volume>
<issue>8</issue>
<fpage>677</fpage>
<lpage>685</lpage>
<pub-id pub-id-type="doi">10.1038/nri1154</pub-id>
<pub-id pub-id-type="pmid">12974482</pub-id>
</element-citation>
</ref>
<ref id="CR21"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Fiser</surname>
<given-names>A</given-names>
</name>
<name><surname>Do</surname>
<given-names>RK</given-names>
</name>
<name><surname>Sali</surname>
<given-names>A</given-names>
</name>
</person-group>
<article-title>Modeling of loops in protein structures</article-title>
<source>Protein Sci</source>
<year>2000</year>
<volume>9</volume>
<fpage>1753</fpage>
<lpage>1773</lpage>
<pub-id pub-id-type="doi">10.1110/ps.9.9.1753</pub-id>
<pub-id pub-id-type="pmid">11045621</pub-id>
</element-citation>
</ref>
<ref id="CR22"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Frieden</surname>
<given-names>TR</given-names>
</name>
<name><surname>Damon</surname>
<given-names>I</given-names>
</name>
<name><surname>Bell</surname>
<given-names>BP</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Ebola 2014—new challenges, new global response and responsibility</article-title>
<source>N Engl J Med</source>
<year>2014</year>
<volume>371</volume>
<issue>13</issue>
<fpage>1177</fpage>
<lpage>1180</lpage>
<pub-id pub-id-type="doi">10.1056/NEJMp1409903</pub-id>
<pub-id pub-id-type="pmid">25140858</pub-id>
</element-citation>
</ref>
<ref id="CR23"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Garcia</surname>
<given-names>KC</given-names>
</name>
<name><surname>Teyton</surname>
<given-names>L</given-names>
</name>
<name><surname>Wilson</surname>
<given-names>IA</given-names>
</name>
</person-group>
<article-title>Structural basis of T cell recognition</article-title>
<source>Annu Rev Immunol</source>
<year>1999</year>
<volume>17</volume>
<fpage>369</fpage>
<lpage>397</lpage>
<pub-id pub-id-type="doi">10.1146/annurev.immunol.17.1.369</pub-id>
<pub-id pub-id-type="pmid">10358763</pub-id>
</element-citation>
</ref>
<ref id="CR24"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Garcia-Boronat</surname>
<given-names>M</given-names>
</name>
<name><surname>Diez-Rivero</surname>
<given-names>CM</given-names>
</name>
<name><surname>Reinherz</surname>
<given-names>EL</given-names>
</name>
<etal></etal>
</person-group>
<article-title>PVS: a web server for protein sequence variability analysis tuned to facilitate conserved epitope discovery</article-title>
<source>Nucleic Acids Res</source>
<year>2008</year>
<volume>36</volume>
<issue>2</issue>
<fpage>35</fpage>
<lpage>41</lpage>
<pub-id pub-id-type="doi">10.1093/nar/gkn211</pub-id>
</element-citation>
</ref>
<ref id="CR25"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gatherer</surname>
<given-names>D</given-names>
</name>
</person-group>
<article-title>The 2014 Ebola virus disease outbreak in West Africa</article-title>
<source>J Gen Virol</source>
<year>2014</year>
<volume>95</volume>
<issue>8</issue>
<fpage>1619</fpage>
<lpage>1624</lpage>
<pub-id pub-id-type="doi">10.1099/vir.0.067199-0</pub-id>
<pub-id pub-id-type="pmid">24795448</pub-id>
</element-citation>
</ref>
<ref id="CR26"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Geisbert</surname>
<given-names>TW</given-names>
</name>
<name><surname>Geisbert</surname>
<given-names>JB</given-names>
</name>
<name><surname>Leung</surname>
<given-names>A</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Single-injection vaccine protects nonhuman primates against infection with marburg virus and three species of ebola virus</article-title>
<source>J Virol</source>
<year>2009</year>
<volume>83</volume>
<issue>14</issue>
<fpage>7296</fpage>
<lpage>7304</lpage>
<pub-id pub-id-type="doi">10.1128/JVI.00561-09</pub-id>
<pub-id pub-id-type="pmid">19386702</pub-id>
</element-citation>
</ref>
<ref id="CR27"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Germain</surname>
<given-names>RN</given-names>
</name>
</person-group>
<article-title>MHC-dependent antigen processing and peptide presentation: providing ligands for T lymphocyte activation</article-title>
<source>Cell</source>
<year>1994</year>
<volume>76</volume>
<issue>2</issue>
<fpage>287</fpage>
<lpage>299</lpage>
<pub-id pub-id-type="doi">10.1016/0092-8674(94)90336-0</pub-id>
<pub-id pub-id-type="pmid">8293464</pub-id>
</element-citation>
</ref>
<ref id="CR28"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Goeijenbier</surname>
<given-names>M</given-names>
</name>
<name><surname>van Kampen</surname>
<given-names>JJ</given-names>
</name>
<name><surname>Reusken</surname>
<given-names>CB</given-names>
</name>
</person-group>
<article-title>Ebola virus disease: a review on epidemiology, symptoms, treatment and pathogenesis</article-title>
<source>Neth J Med</source>
<year>2014</year>
<volume>72</volume>
<issue>9</issue>
<fpage>442</fpage>
<lpage>448</lpage>
<pub-id pub-id-type="pmid">25387613</pub-id>
</element-citation>
</ref>
<ref id="CR29"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hall</surname>
<given-names>TA</given-names>
</name>
</person-group>
<article-title>BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT</article-title>
<source>Nucleic Acids Symp Ser</source>
<year>1999</year>
<volume>41</volume>
<fpage>95</fpage>
<lpage>98</lpage>
</element-citation>
</ref>
<ref id="CR30"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hasan</surname>
<given-names>MA</given-names>
</name>
<name><surname>Hossain</surname>
<given-names>M</given-names>
</name>
<name><surname>Alam</surname>
<given-names>MJ</given-names>
</name>
</person-group>
<article-title>A computational assay to design an epitope-based Peptide vaccine against Saint Louis encephalitis virus</article-title>
<source>Bioinform Biol Insights</source>
<year>2013</year>
<volume>7</volume>
<fpage>347</fpage>
<lpage>355</lpage>
<pub-id pub-id-type="doi">10.4137/BBI.S13402</pub-id>
<pub-id pub-id-type="pmid">24324329</pub-id>
</element-citation>
</ref>
<ref id="CR31"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hasan</surname>
<given-names>MA</given-names>
</name>
<name><surname>Khan</surname>
<given-names>MA</given-names>
</name>
<name><surname>Datta</surname>
<given-names>A</given-names>
</name>
<etal></etal>
</person-group>
<article-title>A comprehensive immunoinformatics and target site study revealedthe corner-stone toward Chikungunya virus treatment</article-title>
<source>Mol Immunol</source>
<year>2015</year>
<volume>65</volume>
<fpage>189</fpage>
<lpage>204</lpage>
<pub-id pub-id-type="doi">10.1016/j.molimm.2014.12.013</pub-id>
<pub-id pub-id-type="pmid">25682054</pub-id>
</element-citation>
</ref>
<ref id="CR32"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hewlett</surname>
<given-names>BL</given-names>
</name>
<name><surname>Hewlett</surname>
<given-names>BS</given-names>
</name>
</person-group>
<article-title>Providing care and facing death: nursing during Ebola outbreaks in central Africa</article-title>
<source>J Transcult Nurs</source>
<year>2005</year>
<volume>16</volume>
<issue>4</issue>
<fpage>289</fpage>
<lpage>297</lpage>
<pub-id pub-id-type="doi">10.1177/1043659605278935</pub-id>
<pub-id pub-id-type="pmid">16160191</pub-id>
</element-citation>
</ref>
<ref id="CR33"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hoenen</surname>
<given-names>T</given-names>
</name>
<name><surname>Groseth</surname>
<given-names>A</given-names>
</name>
<name><surname>Feldmann</surname>
<given-names>H</given-names>
</name>
</person-group>
<article-title>Current Ebola vaccines</article-title>
<source>Expert Opin Biol Ther</source>
<year>2012</year>
<volume>12</volume>
<issue>7</issue>
<fpage>859</fpage>
<lpage>872</lpage>
<pub-id pub-id-type="doi">10.1517/14712598.2012.685152</pub-id>
<pub-id pub-id-type="pmid">22559078</pub-id>
</element-citation>
</ref>
<ref id="CR34"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Holland</surname>
<given-names>J</given-names>
</name>
<name><surname>Domingo</surname>
<given-names>E</given-names>
</name>
</person-group>
<article-title>Origin and evolution of viruses</article-title>
<source>Virus Genes</source>
<year>1998</year>
<volume>16</volume>
<issue>1</issue>
<fpage>13</fpage>
<lpage>21</lpage>
<pub-id pub-id-type="doi">10.1023/A:1007989407305</pub-id>
<pub-id pub-id-type="pmid">9562888</pub-id>
</element-citation>
</ref>
<ref id="CR35"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hoof</surname>
<given-names>I</given-names>
</name>
<name><surname>Peters</surname>
<given-names>B</given-names>
</name>
<name><surname>Sidney</surname>
<given-names>J</given-names>
</name>
<etal></etal>
</person-group>
<article-title>NetMHCpan, a method for MHC class I binding prediction beyond humans</article-title>
<source>Immunogenetics</source>
<year>2009</year>
<volume>61</volume>
<fpage>1</fpage>
<lpage>13</lpage>
<pub-id pub-id-type="doi">10.1007/s00251-008-0341-z</pub-id>
<pub-id pub-id-type="pmid">19002680</pub-id>
</element-citation>
</ref>
<ref id="CR36"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Igietseme</surname>
<given-names>JU</given-names>
</name>
<name><surname>Eko</surname>
<given-names>FO</given-names>
</name>
<name><surname>He</surname>
<given-names>Q</given-names>
</name>
<name><surname>Black</surname>
<given-names>CM</given-names>
</name>
</person-group>
<article-title>Antibody regulation of T-cell immunity: implications for vaccine strategies against intracellular pathogens</article-title>
<source>Expert Rev Vaccines</source>
<year>2004</year>
<volume>3</volume>
<issue>1</issue>
<fpage>23</fpage>
<lpage>34</lpage>
<pub-id pub-id-type="doi">10.1586/14760584.3.1.23</pub-id>
<pub-id pub-id-type="pmid">14761241</pub-id>
</element-citation>
</ref>
<ref id="CR37"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Keiser</surname>
<given-names>J</given-names>
</name>
<name><surname>Utzinger</surname>
<given-names>J</given-names>
</name>
<name><surname>Tanner</surname>
<given-names>M</given-names>
</name>
<name><surname>Singer</surname>
<given-names>BH</given-names>
</name>
</person-group>
<article-title>Representation of authors and editors from countries with different human development indexes in the leading literature on tropical medicine: survey of current evidence</article-title>
<source>BMJ</source>
<year>2004</year>
<volume>328</volume>
<issue>7450</issue>
<fpage>1229</fpage>
<lpage>1232</lpage>
<pub-id pub-id-type="doi">10.1136/bmj.38069.518137.F6</pub-id>
<pub-id pub-id-type="pmid">15059851</pub-id>
</element-citation>
</ref>
<ref id="CR38"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Khan</surname>
<given-names>MK</given-names>
</name>
<name><surname>Zaman</surname>
<given-names>S</given-names>
</name>
<name><surname>Chakraborty</surname>
<given-names>S</given-names>
</name>
<etal></etal>
</person-group>
<article-title>In silico predicted mycobacterial epitope elicits in vitro T-cell responses</article-title>
<source>Mol Immunol</source>
<year>2014</year>
<volume>61</volume>
<issue>1</issue>
<fpage>16</fpage>
<lpage>22</lpage>
<pub-id pub-id-type="doi">10.1016/j.molimm.2014.04.009</pub-id>
<pub-id pub-id-type="pmid">24853589</pub-id>
</element-citation>
</ref>
<ref id="CR39"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Klein</surname>
<given-names>RS</given-names>
</name>
<name><surname>Lin</surname>
<given-names>E</given-names>
</name>
<name><surname>Zhang</surname>
<given-names>B</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Neuronal CXCL10 directs CD8+ T-cell recruitment and control of West Nile virus encephalitis</article-title>
<source>J Virol</source>
<year>2005</year>
<volume>79</volume>
<issue>17</issue>
<fpage>11457</fpage>
<lpage>11466</lpage>
<pub-id pub-id-type="doi">10.1128/JVI.79.17.11457-11466.2005</pub-id>
<pub-id pub-id-type="pmid">16103196</pub-id>
</element-citation>
</ref>
<ref id="CR40"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kolaskar</surname>
<given-names>AS</given-names>
</name>
<name><surname>Tongaonkar</surname>
<given-names>PC</given-names>
</name>
</person-group>
<article-title>A semi-empirical method for prediction of antigenic determinants on protein antigens</article-title>
<source>FEBS Lett</source>
<year>1990</year>
<volume>276</volume>
<issue>1–2</issue>
<fpage>172</fpage>
<lpage>174</lpage>
<pub-id pub-id-type="doi">10.1016/0014-5793(90)80535-Q</pub-id>
<pub-id pub-id-type="pmid">1702393</pub-id>
</element-citation>
</ref>
<ref id="CR41"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kuhn</surname>
<given-names>JH</given-names>
</name>
<name><surname>Becker</surname>
<given-names>S</given-names>
</name>
<name><surname>Ebihara</surname>
<given-names>H</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Proposal for a revised taxonomy of the family Filoviridae: classification, names of taxa and viruses, and virus abbreviations</article-title>
<source>Arch Virol</source>
<year>2010</year>
<volume>155</volume>
<issue>12</issue>
<fpage>2083</fpage>
<lpage>2103</lpage>
<pub-id pub-id-type="doi">10.1007/s00705-010-0814-x</pub-id>
<pub-id pub-id-type="pmid">21046175</pub-id>
</element-citation>
</ref>
<ref id="CR42"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Larsen</surname>
<given-names>MV</given-names>
</name>
<name><surname>Lundegaard</surname>
<given-names>C</given-names>
</name>
<name><surname>Lamberth</surname>
<given-names>K</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Large-scale validation of methods for cytotoxic T-lymphocyte epitope prediction</article-title>
<source>BMC Bioinformatics</source>
<year>2007</year>
<volume>8</volume>
<issue>1</issue>
<fpage>424</fpage>
<pub-id pub-id-type="doi">10.1186/1471-2105-8-424</pub-id>
<pub-id pub-id-type="pmid">17973982</pub-id>
</element-citation>
</ref>
<ref id="CR43"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Laskowski</surname>
<given-names>RA</given-names>
</name>
<name><surname>Rullmann</surname>
<given-names>JAC</given-names>
</name>
<name><surname>MacArthur</surname>
<given-names>MW</given-names>
</name>
<etal></etal>
</person-group>
<article-title>AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR</article-title>
<source>J Biomol NMR</source>
<year>1996</year>
<volume>8</volume>
<issue>4</issue>
<fpage>477</fpage>
<lpage>486</lpage>
<pub-id pub-id-type="doi">10.1007/BF00228148</pub-id>
<pub-id pub-id-type="pmid">9008363</pub-id>
</element-citation>
</ref>
<ref id="CR44"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Leroy</surname>
<given-names>EM</given-names>
</name>
<name><surname>Rouquet</surname>
<given-names>P</given-names>
</name>
<name><surname>Formenty</surname>
<given-names>P</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Multiple Ebola virus transmission events and rapid decline of central African wildlife</article-title>
<source>Science</source>
<year>2004</year>
<volume>303</volume>
<issue>5656</issue>
<fpage>387</fpage>
<lpage>390</lpage>
<pub-id pub-id-type="doi">10.1126/science.1092528</pub-id>
<pub-id pub-id-type="pmid">14726594</pub-id>
</element-citation>
</ref>
<ref id="CR45"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Li</surname>
<given-names>YH</given-names>
</name>
<name><surname>Chen</surname>
<given-names>SP</given-names>
</name>
</person-group>
<article-title>Evolutionary history of Ebola virus</article-title>
<source>Epidemiol Infect</source>
<year>2014</year>
<volume>142</volume>
<issue>06</issue>
<fpage>1138</fpage>
<lpage>1145</lpage>
<pub-id pub-id-type="doi">10.1017/S0950268813002215</pub-id>
<pub-id pub-id-type="pmid">24040779</pub-id>
</element-citation>
</ref>
<ref id="CR46"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Liao</surname>
<given-names>L</given-names>
</name>
<name><surname>Noble</surname>
<given-names>WS</given-names>
</name>
</person-group>
<article-title>Combining pairwise sequence similarity and support vector machines for detecting remote protein evolutionary and structural relationships</article-title>
<source>J Comput Biol</source>
<year>2003</year>
<volume>10</volume>
<issue>6</issue>
<fpage>857</fpage>
<lpage>868</lpage>
<pub-id pub-id-type="doi">10.1089/106652703322756113</pub-id>
<pub-id pub-id-type="pmid">14980014</pub-id>
</element-citation>
</ref>
<ref id="CR47"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>López</surname>
<given-names>JA</given-names>
</name>
<name><surname>Weilenman</surname>
<given-names>C</given-names>
</name>
<name><surname>Audran</surname>
<given-names>R</given-names>
</name>
<etal></etal>
</person-group>
<article-title>A synthetic malaria vaccine elicits a potent CD8+ and CD4+ T lymphocyte immune response in humans. Implications for vaccination strategies</article-title>
<source>Eur J Immunol</source>
<year>2001</year>
<volume>31</volume>
<issue>7</issue>
<fpage>1989</fpage>
<lpage>1998</lpage>
<pub-id pub-id-type="doi">10.1002/1521-4141(200107)31:7<1989::AID-IMMU1989>3.0.CO;2-M</pub-id>
<pub-id pub-id-type="pmid">11449351</pub-id>
</element-citation>
</ref>
<ref id="CR48"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>McKeever</surname>
<given-names>TM</given-names>
</name>
<name><surname>Lewis</surname>
<given-names>SA</given-names>
</name>
<name><surname>Smith</surname>
<given-names>C</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Vaccination and allergic disease: a birth cohort study</article-title>
<source>Am J Public Health</source>
<year>2004</year>
<volume>94</volume>
<issue>6</issue>
<fpage>985</fpage>
<pub-id pub-id-type="doi">10.2105/AJPH.94.6.985</pub-id>
<pub-id pub-id-type="pmid">15249303</pub-id>
</element-citation>
</ref>
<ref id="CR49"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Muh</surname>
<given-names>HC</given-names>
</name>
<name><surname>Tong</surname>
<given-names>JC</given-names>
</name>
<name><surname>Tammi</surname>
<given-names>MT</given-names>
</name>
</person-group>
<article-title>AllerHunter: a SVM-pairwise system for assessment of allergenicity and allergic cross-reactivity in proteins</article-title>
<source>PLoS One</source>
<year>2009</year>
<volume>4</volume>
<issue>6</issue>
<fpage>5861</fpage>
<pub-id pub-id-type="doi">10.1371/journal.pone.0005861</pub-id>
</element-citation>
</ref>
<ref id="CR50"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Mühlberger</surname>
<given-names>E</given-names>
</name>
<name><surname>Lotfering</surname>
<given-names>B</given-names>
</name>
<name><surname>Klenk</surname>
<given-names>H-D</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Three of the four nucleocapsid proteins of Marburg virus, NP, VP35, and L, are sufficient to mediate replication and transcription of Marburg virus-specific monocistronic minigenomes</article-title>
<source>J Virol</source>
<year>1998</year>
<volume>72</volume>
<fpage>8756</fpage>
<lpage>8764</lpage>
<pub-id pub-id-type="pmid">9765419</pub-id>
</element-citation>
</ref>
<ref id="CR51"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Mühlberger</surname>
<given-names>E</given-names>
</name>
<name><surname>Weik</surname>
<given-names>M</given-names>
</name>
<name><surname>Volchkov</surname>
<given-names>V</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Comparison of the transcription and replication strategies of Marburg virus and Ebola virus by using artificial replication systems</article-title>
<source>J Virol</source>
<year>1999</year>
<volume>73</volume>
<fpage>2333</fpage>
<lpage>2342</lpage>
<pub-id pub-id-type="pmid">9971816</pub-id>
</element-citation>
</ref>
<ref id="CR52"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nair</surname>
<given-names>DT</given-names>
</name>
<name><surname>Singh</surname>
<given-names>K</given-names>
</name>
<name><surname>Siddiqui</surname>
<given-names>Z</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Epitope recognition by diverse antibodies suggests conformational convergence in an antibody response</article-title>
<source>J Immunol</source>
<year>2002</year>
<volume>168</volume>
<issue>5</issue>
<fpage>2371</fpage>
<lpage>2382</lpage>
<pub-id pub-id-type="doi">10.4049/jimmunol.168.5.2371</pub-id>
<pub-id pub-id-type="pmid">11859128</pub-id>
</element-citation>
</ref>
<ref id="CR53"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nielsen</surname>
<given-names>M</given-names>
</name>
<name><surname>Lundegaard</surname>
<given-names>C</given-names>
</name>
<name><surname>Blicher</surname>
<given-names>T</given-names>
</name>
<etal></etal>
</person-group>
<article-title>NetMHCpan, a method for quantitative predictions of peptide binding to any HLA-A and -B locus protein of known sequence</article-title>
<source>PLoS One</source>
<year>2007</year>
<volume>2</volume>
<fpage>796</fpage>
<pub-id pub-id-type="doi">10.1371/journal.pone.0000796</pub-id>
</element-citation>
</ref>
<ref id="CR54"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Oany</surname>
<given-names>AR</given-names>
</name>
<name><surname>Emran</surname>
<given-names>AA</given-names>
</name>
<name><surname>Jyoti</surname>
<given-names>TP</given-names>
</name>
</person-group>
<article-title>Design of an epitope-based peptide vaccine against spike protein of human corona virus: an in silico approach</article-title>
<source>Drug Des Devel Ther</source>
<year>2008</year>
<volume>8</volume>
<fpage>1139</fpage>
<lpage>1149</lpage>
</element-citation>
</ref>
<ref id="CR55"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Oany</surname>
<given-names>AR</given-names>
</name>
<name><surname>Ahmad</surname>
<given-names>SAI</given-names>
</name>
<name><surname>Hossain</surname>
<given-names>MU</given-names>
</name>
<name><surname>Jyoti</surname>
<given-names>TP</given-names>
</name>
</person-group>
<article-title>Identification of highly conserved regions in L-segment of Crimean–Congo hemorrhagic fever virus and immunoinformatic prediction about potential novel vaccine</article-title>
<source>Adv Appl Bioinformatics Chem</source>
<year>2015</year>
<volume>8</volume>
<fpage>1</fpage>
<lpage>10</lpage>
<pub-id pub-id-type="doi">10.2147/AABC.S75250</pub-id>
</element-citation>
</ref>
<ref id="CR56"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Parker</surname>
<given-names>JM</given-names>
</name>
<name><surname>Guo</surname>
<given-names>D</given-names>
</name>
<name><surname>Hodges</surname>
<given-names>RS</given-names>
</name>
</person-group>
<article-title>New hydrophilicity scale derived from high-performance liquid chromatography peptide retention data: correlation of predicted surface residues with antigenicity and X-ray-derived accessible sites</article-title>
<source>Biochemistry</source>
<year>1986</year>
<volume>25</volume>
<fpage>5425</fpage>
<lpage>5432</lpage>
<pub-id pub-id-type="doi">10.1021/bi00367a013</pub-id>
<pub-id pub-id-type="pmid">2430611</pub-id>
</element-citation>
</ref>
<ref id="CR57"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Peters</surname>
<given-names>CJ</given-names>
</name>
<name><surname>Peters</surname>
<given-names>JW</given-names>
</name>
</person-group>
<article-title>An introduction to Ebola: the virus and the disease</article-title>
<source>J Infect Dis</source>
<year>1999</year>
<volume>179</volume>
<issue>1</issue>
<fpage>ix</fpage>
<lpage>xvi</lpage>
<pub-id pub-id-type="pmid">9988154</pub-id>
</element-citation>
</ref>
<ref id="CR58"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Peters</surname>
<given-names>B</given-names>
</name>
<name><surname>Sette</surname>
<given-names>A</given-names>
</name>
</person-group>
<article-title>Generating quantitative models describing the sequence specificity of biological processes with the stabilized matrix method</article-title>
<source>BMC Bioinformatics</source>
<year>2005</year>
<volume>6</volume>
<issue>1</issue>
<fpage>132</fpage>
<pub-id pub-id-type="doi">10.1186/1471-2105-6-132</pub-id>
<pub-id pub-id-type="pmid">15927070</pub-id>
</element-citation>
</ref>
<ref id="CR59"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Petrovsky</surname>
<given-names>N</given-names>
</name>
<name><surname>Brusic</surname>
<given-names>V</given-names>
</name>
</person-group>
<article-title>Computational immunology: the coming of age</article-title>
<source>Immunol Cell Biol</source>
<year>2002</year>
<volume>80</volume>
<issue>3</issue>
<fpage>248</fpage>
<lpage>254</lpage>
<pub-id pub-id-type="doi">10.1046/j.1440-1711.2002.01093.x</pub-id>
<pub-id pub-id-type="pmid">12067412</pub-id>
</element-citation>
</ref>
<ref id="CR60"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Poch</surname>
<given-names>O</given-names>
</name>
<name><surname>Blumberg</surname>
<given-names>BM</given-names>
</name>
<name><surname>Bougueleret</surname>
<given-names>L</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains</article-title>
<source>J Gen Virol</source>
<year>1990</year>
<volume>71</volume>
<fpage>1153</fpage>
<lpage>1162</lpage>
<pub-id pub-id-type="doi">10.1099/0022-1317-71-5-1153</pub-id>
<pub-id pub-id-type="pmid">2161049</pub-id>
</element-citation>
</ref>
<ref id="CR61"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Poland</surname>
<given-names>GA</given-names>
</name>
<name><surname>Ovsyannikova</surname>
<given-names>IG</given-names>
</name>
<name><surname>Jacobson</surname>
<given-names>RM</given-names>
</name>
</person-group>
<article-title>Application of pharmacogenomics to vaccines</article-title>
<source>Pharmacogenomics</source>
<year>2009</year>
<volume>10</volume>
<issue>5</issue>
<fpage>837</fpage>
<lpage>852</lpage>
<pub-id pub-id-type="doi">10.2217/pgs.09.25</pub-id>
<pub-id pub-id-type="pmid">19450131</pub-id>
</element-citation>
</ref>
<ref id="CR62"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rini</surname>
<given-names>JM</given-names>
</name>
<name><surname>Schulze-Gahmen</surname>
<given-names>U</given-names>
</name>
<name><surname>Wilson</surname>
<given-names>IA</given-names>
</name>
</person-group>
<article-title>Structural evidence for induced fit as a mechanism for antibody-antigen recognition</article-title>
<source>Science</source>
<year>1992</year>
<volume>255</volume>
<issue>5047</issue>
<fpage>959</fpage>
<lpage>965</lpage>
<pub-id pub-id-type="doi">10.1126/science.1546293</pub-id>
<pub-id pub-id-type="pmid">1546293</pub-id>
</element-citation>
</ref>
<ref id="CR63"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Robinson</surname>
<given-names>HL</given-names>
</name>
<name><surname>Amara</surname>
<given-names>RR</given-names>
</name>
</person-group>
<article-title>T cell vaccines for microbial infections</article-title>
<source>Nat Med</source>
<year>2005</year>
<volume>11</volume>
<fpage>25</fpage>
<lpage>32</lpage>
<pub-id pub-id-type="doi">10.1038/nm1212</pub-id>
</element-citation>
</ref>
<ref id="CR64"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rouquet</surname>
<given-names>P</given-names>
</name>
<name><surname>Froment</surname>
<given-names>JM</given-names>
</name>
<name><surname>Bermejo</surname>
<given-names>M</given-names>
</name>
</person-group>
<article-title>Wild animal mortality monitoring and human Ebola outbreaks, Gabon and Republic of Congo, 2001–2003</article-title>
<source>Emerg Infect Dis</source>
<year>2005</year>
<volume>11</volume>
<issue>2</issue>
<fpage>283</fpage>
<lpage>290</lpage>
<pub-id pub-id-type="doi">10.3201/eid1102.040533</pub-id>
<pub-id pub-id-type="pmid">15752448</pub-id>
</element-citation>
</ref>
<ref id="CR65"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sali</surname>
<given-names>A</given-names>
</name>
<name><surname>Blundell</surname>
<given-names>TL</given-names>
</name>
</person-group>
<article-title>Comparative protein modelling by satisfaction of spatial restraints</article-title>
<source>J Mol Biol</source>
<year>1993</year>
<volume>234</volume>
<fpage>779</fpage>
<lpage>815</lpage>
<pub-id pub-id-type="doi">10.1006/jmbi.1993.1626</pub-id>
<pub-id pub-id-type="pmid">8254673</pub-id>
</element-citation>
</ref>
<ref id="CR66"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Šali</surname>
<given-names>A</given-names>
</name>
<name><surname>Potterton</surname>
<given-names>L</given-names>
</name>
<name><surname>Yuan</surname>
<given-names>F</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Evaluation of comparative protein modeling by MODELLER</article-title>
<source>Proteins</source>
<year>1995</year>
<volume>23</volume>
<issue>3</issue>
<fpage>318</fpage>
<lpage>326</lpage>
<pub-id pub-id-type="doi">10.1002/prot.340230306</pub-id>
<pub-id pub-id-type="pmid">8710825</pub-id>
</element-citation>
</ref>
<ref id="CR67"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sette</surname>
<given-names>A</given-names>
</name>
<name><surname>Fikes</surname>
<given-names>J</given-names>
</name>
</person-group>
<article-title>Epitope-based vaccines: an update on epitope identification, vaccine design and delivery</article-title>
<source>Curr Opin Immunol</source>
<year>2003</year>
<volume>15</volume>
<issue>4</issue>
<fpage>461</fpage>
<lpage>470</lpage>
<pub-id pub-id-type="doi">10.1016/S0952-7915(03)00083-9</pub-id>
<pub-id pub-id-type="pmid">12900280</pub-id>
</element-citation>
</ref>
<ref id="CR68"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sette</surname>
<given-names>A</given-names>
</name>
<name><surname>Newman</surname>
<given-names>M</given-names>
</name>
<name><surname>Livingston</surname>
<given-names>B</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Optimizing vaccine design for cellular processing, MHC binding and TCR recognition</article-title>
<source>Tissue Antigens</source>
<year>2002</year>
<volume>59</volume>
<issue>6</issue>
<fpage>443</fpage>
<lpage>451</lpage>
<pub-id pub-id-type="doi">10.1034/j.1399-0039.2002.590601.x</pub-id>
<pub-id pub-id-type="pmid">12445314</pub-id>
</element-citation>
</ref>
<ref id="CR69"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Shrestha</surname>
<given-names>B</given-names>
</name>
<name><surname>Diamond</surname>
<given-names>MS</given-names>
</name>
</person-group>
<article-title>Role of CD8+ T cells in control of West Nile virus infection</article-title>
<source>J Virol</source>
<year>2004</year>
<volume>78</volume>
<issue>15</issue>
<fpage>8312</fpage>
<lpage>8321</lpage>
<pub-id pub-id-type="doi">10.1128/JVI.78.15.8312-8321.2004</pub-id>
<pub-id pub-id-type="pmid">15254203</pub-id>
</element-citation>
</ref>
<ref id="CR70"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sullivan</surname>
<given-names>NJ</given-names>
</name>
<name><surname>Sanchez</surname>
<given-names>A</given-names>
</name>
<name><surname>Rollin</surname>
<given-names>PE</given-names>
</name>
<name><surname>Yang</surname>
<given-names>ZY</given-names>
</name>
<name><surname>Nabel</surname>
<given-names>GJ</given-names>
</name>
</person-group>
<article-title>Development of a preventive vaccine for Ebola virus infection in primates</article-title>
<source>Nature</source>
<year>2000</year>
<volume>408</volume>
<fpage>605</fpage>
<lpage>609</lpage>
<pub-id pub-id-type="doi">10.1038/35046108</pub-id>
<pub-id pub-id-type="pmid">11117750</pub-id>
</element-citation>
</ref>
<ref id="CR71"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Takada</surname>
<given-names>A</given-names>
</name>
<name><surname>Robison</surname>
<given-names>C</given-names>
</name>
<name><surname>Goto</surname>
<given-names>H</given-names>
</name>
<etal></etal>
</person-group>
<article-title>A system for functional analysis of Ebola virus glycoprotein</article-title>
<source>Proc Natl Acad Sci</source>
<year>1997</year>
<volume>94</volume>
<issue>26</issue>
<fpage>14764</fpage>
<lpage>14769</lpage>
<pub-id pub-id-type="doi">10.1073/pnas.94.26.14764</pub-id>
<pub-id pub-id-type="pmid">9405687</pub-id>
</element-citation>
</ref>
<ref id="CR72"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Thompson</surname>
<given-names>JD</given-names>
</name>
<name><surname>Higgins</surname>
<given-names>DG</given-names>
</name>
<name><surname>Gibson</surname>
<given-names>TJ</given-names>
</name>
</person-group>
<article-title>CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice</article-title>
<source>Nucleic Acids Res</source>
<year>1994</year>
<volume>22</volume>
<issue>22</issue>
<fpage>4673</fpage>
<lpage>4680</lpage>
<pub-id pub-id-type="doi">10.1093/nar/22.22.4673</pub-id>
<pub-id pub-id-type="pmid">7984417</pub-id>
</element-citation>
</ref>
<ref id="CR73"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Trainor</surname>
<given-names>NB</given-names>
</name>
<name><surname>Crill</surname>
<given-names>WD</given-names>
</name>
<name><surname>Roberson</surname>
<given-names>JA</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Mutation analysis of the fusion domain region of St. Louis encephalitis virus envelope protein</article-title>
<source>Virology</source>
<year>2007</year>
<volume>360</volume>
<issue>2</issue>
<fpage>398</fpage>
<lpage>406</lpage>
<pub-id pub-id-type="doi">10.1016/j.virol.2006.10.033</pub-id>
<pub-id pub-id-type="pmid">17157348</pub-id>
</element-citation>
</ref>
<ref id="CR74"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Twiddy</surname>
<given-names>SS</given-names>
</name>
<name><surname>Holmes</surname>
<given-names>EC</given-names>
</name>
<name><surname>Rambaut</surname>
<given-names>A</given-names>
</name>
</person-group>
<article-title>Inferring the rate and time-scale of dengue virus evolution</article-title>
<source>Mol Biol Evol</source>
<year>2003</year>
<volume>20</volume>
<issue>1</issue>
<fpage>122</fpage>
<lpage>129</lpage>
<pub-id pub-id-type="doi">10.1093/molbev/msg010</pub-id>
<pub-id pub-id-type="pmid">12519914</pub-id>
</element-citation>
</ref>
<ref id="CR75"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Volchkov</surname>
<given-names>VE</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Characterization of the L gene and 5′trailer region of Ebola virus</article-title>
<source>J Gen Virol</source>
<year>1999</year>
<volume>80</volume>
<issue>2</issue>
<fpage>355</fpage>
<lpage>362</lpage>
<pub-id pub-id-type="pmid">10073695</pub-id>
</element-citation>
</ref>
<ref id="CR76"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname>
<given-names>P</given-names>
</name>
<name><surname>Sidney</surname>
<given-names>J</given-names>
</name>
<name><surname>Dow</surname>
<given-names>C</given-names>
</name>
<etal></etal>
</person-group>
<article-title>A systematic assessment of MHC class II peptide binding predictions and evaluation of a consensus approach</article-title>
<source>PLoS Comput Biol</source>
<year>2008</year>
<volume>4</volume>
<issue>4</issue>
<fpage>e1000048</fpage>
<pub-id pub-id-type="doi">10.1371/journal.pcbi.1000048</pub-id>
<pub-id pub-id-type="pmid">18389056</pub-id>
</element-citation>
</ref>
<ref id="CR77"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname>
<given-names>P</given-names>
</name>
<name><surname>Sidney</surname>
<given-names>J</given-names>
</name>
<name><surname>Kim</surname>
<given-names>Y</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Peptide binding predictions for HLA DR, DP and DQ molecules</article-title>
<source>BMC Bioinformatics</source>
<year>2010</year>
<volume>11</volume>
<issue>1</issue>
<fpage>568</fpage>
<pub-id pub-id-type="doi">10.1186/1471-2105-11-568</pub-id>
<pub-id pub-id-type="pmid">21092157</pub-id>
</element-citation>
</ref>
<ref id="CR78"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ward</surname>
<given-names>JJ</given-names>
</name>
<name><surname>McGuffin</surname>
<given-names>LJ</given-names>
</name>
<name><surname>Bryson</surname>
<given-names>K</given-names>
</name>
<etal></etal>
</person-group>
<article-title>The DISOPRED server for the prediction of protein disorder</article-title>
<source>Bioinformatics</source>
<year>2004</year>
<volume>20</volume>
<issue>13</issue>
<fpage>2138</fpage>
<lpage>2139</lpage>
<pub-id pub-id-type="doi">10.1093/bioinformatics/bth195</pub-id>
<pub-id pub-id-type="pmid">15044227</pub-id>
</element-citation>
</ref>
<ref id="CR79"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Waterhouse</surname>
<given-names>AM</given-names>
</name>
<name><surname>Procter</surname>
<given-names>JB</given-names>
</name>
<name><surname>Martin</surname>
<given-names>DM</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Jalview version 2 – a multiple sequence alignment editor and analysis workbench</article-title>
<source>Bioinformatics</source>
<year>2009</year>
<volume>25</volume>
<issue>9</issue>
<fpage>1189</fpage>
<lpage>1191</lpage>
<pub-id pub-id-type="doi">10.1093/bioinformatics/btp033</pub-id>
<pub-id pub-id-type="pmid">19151095</pub-id>
</element-citation>
</ref>
<ref id="CR80"><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wilson</surname>
<given-names>CC</given-names>
</name>
<name><surname>McKinney</surname>
<given-names>D</given-names>
</name>
<name><surname>Anders</surname>
<given-names>M</given-names>
</name>
<etal></etal>
</person-group>
<article-title>Development of a DNA vaccine designed to induce cytotoxic T lymphocyte responses to multiple conserved epitopes in HIV-1</article-title>
<source>J Immunol</source>
<year>2003</year>
<volume>171</volume>
<issue>10</issue>
<fpage>5611</fpage>
<lpage>5623</lpage>
<pub-id pub-id-type="doi">10.4049/jimmunol.171.10.5611</pub-id>
<pub-id pub-id-type="pmid">14607970</pub-id>
</element-citation>
</ref>
</ref-list>
</back>
</pmc>
</record>
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