Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid detection of tomato leaf curl Bengaluru virus through loop mediated isothermal amplification assay</title>
<author><name sortKey="Arutselvan, R" sort="Arutselvan, R" uniqKey="Arutselvan R" first="R." last="Arutselvan">R. Arutselvan</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2169 875X</institution-id>
<institution-id institution-id-type="GRID">grid.418373.a</institution-id>
<institution>ICAR, Central Tuber Crops Research Institute,</institution>
</institution-wrap>
Thiruvananthapuram, Kerala 695 017 India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Krishna Reddy, M" sort="Krishna Reddy, M" uniqKey="Krishna Reddy M" first="M." last="Krishna Reddy">M. Krishna Reddy</name>
<affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8663 7600</institution-id>
<institution-id institution-id-type="GRID">grid.418222.f</institution-id>
<institution>ICAR, Indian Institute of Horticultural Research,</institution>
</institution-wrap>
Bengaluru, 560 089 India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Makeshkumar, T" sort="Makeshkumar, T" uniqKey="Makeshkumar T" first="T." last="Makeshkumar">T. Makeshkumar</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2169 875X</institution-id>
<institution-id institution-id-type="GRID">grid.418373.a</institution-id>
<institution>ICAR, Central Tuber Crops Research Institute,</institution>
</institution-wrap>
Thiruvananthapuram, Kerala 695 017 India</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">29291217</idno>
<idno type="pmc">5684993</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684993</idno>
<idno type="RBID">PMC:5684993</idno>
<idno type="doi">10.1007/s13337-017-0385-5</idno>
<date when="2017">2017</date>
<idno type="wicri:Area/Pmc/Corpus">000158</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000158</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Rapid detection of tomato leaf curl Bengaluru virus through loop mediated isothermal amplification assay</title>
<author><name sortKey="Arutselvan, R" sort="Arutselvan, R" uniqKey="Arutselvan R" first="R." last="Arutselvan">R. Arutselvan</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2169 875X</institution-id>
<institution-id institution-id-type="GRID">grid.418373.a</institution-id>
<institution>ICAR, Central Tuber Crops Research Institute,</institution>
</institution-wrap>
Thiruvananthapuram, Kerala 695 017 India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Krishna Reddy, M" sort="Krishna Reddy, M" uniqKey="Krishna Reddy M" first="M." last="Krishna Reddy">M. Krishna Reddy</name>
<affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8663 7600</institution-id>
<institution-id institution-id-type="GRID">grid.418222.f</institution-id>
<institution>ICAR, Indian Institute of Horticultural Research,</institution>
</institution-wrap>
Bengaluru, 560 089 India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Makeshkumar, T" sort="Makeshkumar, T" uniqKey="Makeshkumar T" first="T." last="Makeshkumar">T. Makeshkumar</name>
<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2169 875X</institution-id>
<institution-id institution-id-type="GRID">grid.418373.a</institution-id>
<institution>ICAR, Central Tuber Crops Research Institute,</institution>
</institution-wrap>
Thiruvananthapuram, Kerala 695 017 India</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">VirusDisease</title>
<idno type="ISSN">2347-3584</idno>
<idno type="eISSN">2347-3517</idno>
<imprint><date when="2017">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="Par1">A loop-mediated isothermal amplification (LAMP) technique was employed to develop a simple and rapid method for the detection of tomato leaf curl Bangalore virus (ToLCBaV) in diseased plants of tomato (<italic>Solanum lycopersicum</italic>
). Six sets of primers were designed for LAMP technique targeting the conserved AC1 region and successfully detected ToLCBaV. No reaction was detected in the tissues of healthy plants by either the LAMP or the polymerase chain reaction (PCR). The LAMP products can be visualized by presence or absence of turbidity and staining (0.2 μL for 25 μL LAMP product) directly in the tube with nucleic acid stain dye which allowed easy detection. Sensitivity of LAMP assay is 100 times of conventional PCR technique. Although, both the LAMP and the PCR methods were capable of detecting ToLCBaV in infected tissues of tomato, the LAMP method would be more useful than the PCR method for detection of ToLCBaV infection in tomato plants because it is more rapid, simple and accurate method.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Virusdisease</journal-id>
<journal-id journal-id-type="iso-abbrev">Virusdisease</journal-id>
<journal-title-group><journal-title>VirusDisease</journal-title>
</journal-title-group>
<issn pub-type="ppub">2347-3584</issn>
<issn pub-type="epub">2347-3517</issn>
<publisher><publisher-name>Springer India</publisher-name>
<publisher-loc>New Delhi</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">29291217</article-id>
<article-id pub-id-type="pmc">5684993</article-id>
<article-id pub-id-type="publisher-id">385</article-id>
<article-id pub-id-type="doi">10.1007/s13337-017-0385-5</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Rapid detection of tomato leaf curl Bengaluru virus through loop mediated isothermal amplification assay</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Arutselvan</surname>
<given-names>R.</given-names>
</name>
<address><email>rarutselvan@gmail.com</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Krishna Reddy</surname>
<given-names>M.</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Makeshkumar</surname>
<given-names>T.</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<aff id="Aff1"><label>1</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2169 875X</institution-id>
<institution-id institution-id-type="GRID">grid.418373.a</institution-id>
<institution>ICAR, Central Tuber Crops Research Institute,</institution>
</institution-wrap>
Thiruvananthapuram, Kerala 695 017 India</aff>
<aff id="Aff2"><label>2</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0000 8663 7600</institution-id>
<institution-id institution-id-type="GRID">grid.418222.f</institution-id>
<institution>ICAR, Indian Institute of Horticultural Research,</institution>
</institution-wrap>
Bengaluru, 560 089 India</aff>
</contrib-group>
<pub-date pub-type="epub"><day>13</day>
<month>6</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="ppub"><month>9</month>
<year>2017</year>
</pub-date>
<volume>28</volume>
<issue>3</issue>
<fpage>303</fpage>
<lpage>308</lpage>
<history><date date-type="received"><day>1</day>
<month>2</month>
<year>2017</year>
</date>
<date date-type="accepted"><day>30</day>
<month>5</month>
<year>2017</year>
</date>
</history>
<permissions><copyright-statement>© Indian Virological Society 2017</copyright-statement>
</permissions>
<abstract id="Abs1"><p id="Par1">A loop-mediated isothermal amplification (LAMP) technique was employed to develop a simple and rapid method for the detection of tomato leaf curl Bangalore virus (ToLCBaV) in diseased plants of tomato (<italic>Solanum lycopersicum</italic>
). Six sets of primers were designed for LAMP technique targeting the conserved AC1 region and successfully detected ToLCBaV. No reaction was detected in the tissues of healthy plants by either the LAMP or the polymerase chain reaction (PCR). The LAMP products can be visualized by presence or absence of turbidity and staining (0.2 μL for 25 μL LAMP product) directly in the tube with nucleic acid stain dye which allowed easy detection. Sensitivity of LAMP assay is 100 times of conventional PCR technique. Although, both the LAMP and the PCR methods were capable of detecting ToLCBaV in infected tissues of tomato, the LAMP method would be more useful than the PCR method for detection of ToLCBaV infection in tomato plants because it is more rapid, simple and accurate method.</p>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>LAMP</kwd>
<kwd>ToLCBaV</kwd>
<kwd>Turbidity</kwd>
<kwd>Nucleic acid stain</kwd>
<kwd>Sensitivity</kwd>
</kwd-group>
<funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">http://dx.doi.org/10.13039/501100001503</institution-id>
<institution>Indian Council of Agricultural Research</institution>
</institution-wrap>
</funding-source>
<award-id>ICAR- Consortia Research Platform (CRP) on Vaccines and diagnostics. F. No. 16-18/PP/ICAR CRP/16-17/18</award-id>
<principal-award-recipient><name><surname>Krishna Reddy</surname>
<given-names>M.</given-names>
</name>
</principal-award-recipient>
</award-group>
</funding-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© Indian Virological Society 2017</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 0001589 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 0001589 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV2 |flux= Pmc |étape= Corpus |type= RBID |clé= |texte= }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |