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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cooperation of an RNA Packaging Signal and a Viral Envelope Protein in Coronavirus RNA Packaging</title>
<author><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name>
</author>
<author><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">11533169</idno>
<idno type="pmc">114474</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC114474</idno>
<idno type="RBID">PMC:114474</idno>
<idno type="doi">10.1128/JVI.75.19.9059-9067.2001</idno>
<date when="2001">2001</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cooperation of an RNA Packaging Signal and a Viral Envelope Protein in Coronavirus RNA Packaging</title>
<author><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name>
</author>
<author><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2001">2001</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
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<front><div type="abstract" xml:lang="en"><p>Murine coronavirus mouse hepatitis virus (MHV) produces a genome-length mRNA, mRNA 1, and six or seven species of subgenomic mRNAs in infected cells. Among these mRNAs, only mRNA 1 is efficiently packaged into MHV particles. MHV N protein binds to all MHV mRNAs, whereas envelope M protein interacts only with mRNA 1. This M protein-mRNA 1 interaction most probably determines the selective packaging of mRNA 1 into MHV particles. A short <italic>cis</italic>
-acting MHV RNA packaging signal is necessary and sufficient for packaging RNA into MHV particles. The present study tested the possibility that the selective M protein-mRNA 1 interaction is due to the packaging signal in mRNA 1. Regardless of the presence or absence of the packaging signal, N protein bound to MHV defective interfering RNAs and intracellularly expressed non-MHV RNA transcripts to form ribonucleoprotein complexes; M protein, however, interacted selectively with RNAs containing the packaging signal. Moreover, only the RNA that interacted selectively with M protein was efficiently packaged into MHV particles. Thus, it was the packaging signal that mediated the selective interaction between M protein and viral RNA to drive the specific packaging of RNA into virus particles. This is the first example for any RNA virus in which a viral envelope protein and a known viral RNA packaging signal have been shown to determine the specificity and selectivity of RNA packaging into virions.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id>
<journal-id journal-id-type="publisher-id">J VIROL</journal-id>
<journal-title>Journal of Virology</journal-title>
<issn pub-type="ppub">0022-538X</issn>
<issn pub-type="epub">1098-5514</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">11533169</article-id>
<article-id pub-id-type="pmc">114474</article-id>
<article-id pub-id-type="publisher-id">0451</article-id>
<article-id pub-id-type="doi">10.1128/JVI.75.19.9059-9067.2001</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Structure and Assembly</subject>
</subj-group>
</article-categories>
<title-group><article-title>Cooperation of an RNA Packaging Signal and a Viral Envelope Protein in Coronavirus RNA Packaging</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Narayanan</surname>
<given-names>Krishna</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Makino</surname>
<given-names>Shinji</given-names>
</name>
<xref ref-type="author-notes" rid="FN150">*</xref>
</contrib>
</contrib-group>
<aff id="N0x9cf0138.0xa1b7f28">Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1019, and Department of Microbiology and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712-1095</aff>
<author-notes><fn id="FN150"><label>*</label>
<p>Corresponding author. Mailing address: Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555-1019. Phone: (409) 772-2323. Fax: (409) 772-5065. E-mail: <email>shmakino@utmb.edu</email>
.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>10</month>
<year>2001</year>
</pub-date>
<volume>75</volume>
<issue>19</issue>
<fpage>9059</fpage>
<lpage>9067</lpage>
<history><date date-type="received"><day>7</day>
<month>3</month>
<year>2001</year>
</date>
<date date-type="accepted"><day>5</day>
<month>7</month>
<year>2001</year>
</date>
</history>
<copyright-statement>Copyright © 2001, American Society for Microbiology</copyright-statement>
<copyright-year>2001</copyright-year>
<abstract><p>Murine coronavirus mouse hepatitis virus (MHV) produces a genome-length mRNA, mRNA 1, and six or seven species of subgenomic mRNAs in infected cells. Among these mRNAs, only mRNA 1 is efficiently packaged into MHV particles. MHV N protein binds to all MHV mRNAs, whereas envelope M protein interacts only with mRNA 1. This M protein-mRNA 1 interaction most probably determines the selective packaging of mRNA 1 into MHV particles. A short <italic>cis</italic>
-acting MHV RNA packaging signal is necessary and sufficient for packaging RNA into MHV particles. The present study tested the possibility that the selective M protein-mRNA 1 interaction is due to the packaging signal in mRNA 1. Regardless of the presence or absence of the packaging signal, N protein bound to MHV defective interfering RNAs and intracellularly expressed non-MHV RNA transcripts to form ribonucleoprotein complexes; M protein, however, interacted selectively with RNAs containing the packaging signal. Moreover, only the RNA that interacted selectively with M protein was efficiently packaged into MHV particles. Thus, it was the packaging signal that mediated the selective interaction between M protein and viral RNA to drive the specific packaging of RNA into virus particles. This is the first example for any RNA virus in which a viral envelope protein and a known viral RNA packaging signal have been shown to determine the specificity and selectivity of RNA packaging into virions.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>
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