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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Presence of Oligoclonal T Cells in Cerebrospinal Fluid of a Child with Multiphasic Disseminated Encephalomyelitis following Hepatitis A Virus Infection</title><author><name sortKey="Oleszak, Emilia L" sort="Oleszak, Emilia L" uniqKey="Oleszak E" first="Emilia L." last="Oleszak">Emilia L. Oleszak</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Lin, Wan Lu" sort="Lin, Wan Lu" uniqKey="Lin W" first="Wan Lu" last="Lin">Wan Lu Lin</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Legido, Agustin" sort="Legido, Agustin" uniqKey="Legido A" first="Agustin" last="Legido">Agustin Legido</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Melvin, Joseph" sort="Melvin, Joseph" uniqKey="Melvin J" first="Joseph" last="Melvin">Joseph Melvin</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Hardison, Huntley" sort="Hardison, Huntley" uniqKey="Hardison H" first="Huntley" last="Hardison">Huntley Hardison</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Hoffman, Brad E" sort="Hoffman, Brad E" uniqKey="Hoffman B" first="Brad E." last="Hoffman">Brad E. Hoffman</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Katsetos, Christos D" sort="Katsetos, Christos D" uniqKey="Katsetos C" first="Christos D." last="Katsetos">Christos D. Katsetos</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Platsoucas, Chris D" sort="Platsoucas, Chris D" uniqKey="Platsoucas C" first="Chris D." last="Platsoucas">Chris D. Platsoucas</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">11527815</idno><idno type="pmc">96183</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC96183</idno><idno type="RBID">PMC:96183</idno><idno type="doi">10.1128/CDLI.8.5.984-992.2001</idno><date when="2001">2001</date><idno type="wicri:Area/Pmc/Corpus">000146</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000146</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Presence of Oligoclonal T Cells in Cerebrospinal Fluid of a Child with Multiphasic Disseminated Encephalomyelitis following Hepatitis A Virus Infection</title><author><name sortKey="Oleszak, Emilia L" sort="Oleszak, Emilia L" uniqKey="Oleszak E" first="Emilia L." last="Oleszak">Emilia L. Oleszak</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Lin, Wan Lu" sort="Lin, Wan Lu" uniqKey="Lin W" first="Wan Lu" last="Lin">Wan Lu Lin</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Legido, Agustin" sort="Legido, Agustin" uniqKey="Legido A" first="Agustin" last="Legido">Agustin Legido</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Melvin, Joseph" sort="Melvin, Joseph" uniqKey="Melvin J" first="Joseph" last="Melvin">Joseph Melvin</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Hardison, Huntley" sort="Hardison, Huntley" uniqKey="Hardison H" first="Huntley" last="Hardison">Huntley Hardison</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Hoffman, Brad E" sort="Hoffman, Brad E" uniqKey="Hoffman B" first="Brad E." last="Hoffman">Brad E. Hoffman</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Katsetos, Christos D" sort="Katsetos, Christos D" uniqKey="Katsetos C" first="Christos D." last="Katsetos">Christos D. Katsetos</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author><author><name sortKey="Platsoucas, Chris D" sort="Platsoucas, Chris D" uniqKey="Platsoucas C" first="Chris D." last="Platsoucas">Chris D. Platsoucas</name><affiliation><nlm:aff id="N0x9771d58.0x96e5ca0"></nlm:aff></affiliation></author></analytic><series><title level="j">Clinical and Diagnostic Laboratory Immunology</title><idno type="ISSN">1071-412X</idno><idno type="eISSN">1098-6588</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We have investigated the clonality of β-chain T-cell receptor (TCR) transcripts from the cerebrospinal fluid (CSF) and peripheral blood from a 7-year old child who developed a multiphasic disseminated encephalomyelitis following an infection with hepatitis A virus. We amplified β-chain TCR transcripts by nonpalindromic adaptor (NPA)-PCR–Vβ-specific PCR. TCR transcripts from only five Vβ families (Vβ13, Vβ3, Vβ17, Vβ8, and Vβ20) were detected in CSF. The amplified products were combined, cloned, and sequenced. Sequence analysis revealed in the CSF substantial proportions of identical β-chain of TCR transcripts, demonstrating oligoclonal populations of T cells. Seventeen of 35 (48%) transcripts were 100% identical, demonstrating a major Vβ13.3 Dβ2.1 Jβ1.3 clonal expansion. Six of 35 (17%) transcripts were also 100% identical, revealing a second Vβ13 clonal expansion (Vβ13.1 Dβ2.1 Jβ1.2). Clonal expansions were also found within the Vβ3 family (transcript Vβ3.1 Dβ2.1 Jβ1.5 accounted for 5 of 35 transcripts [14%]) and within the Vβ20 family (transcript Vβ20.1 Dβ1.1 Jβ2.4 accounted for 3 of 35 transcripts [8%]). These results demonstrate the presence of T-cell oligoclonal expansions in the CSF of this patient following infection with hepatitis A virus. Analysis of the CDR3 motifs revealed that two of the clonally expanded T-cell clones exhibited substantial homology to myelin basic protein-reactive T-cell clones. In contrast, all Vβ TCR families were expressed in peripheral blood lymphocytes. Oligoclonal expansions of T cells were not detected in the peripheral blood of this patient. It remains to be determined whether these clonally expanded T cells are specific for hepatitis A viral antigen(s) or host central nervous system antigen(s) and whether molecular mimicry between hepatitis A viral protein and a host protein is responsible for demyelinating disease in this patient.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Clin Diagn Lab Immunol</journal-id><journal-id journal-id-type="publisher-id">CLIN DIAGN LAB IMMUNOL</journal-id><journal-title>Clinical and Diagnostic Laboratory Immunology</journal-title><issn pub-type="ppub">1071-412X</issn><issn pub-type="epub">1098-6588</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">11527815</article-id><article-id pub-id-type="pmc">96183</article-id><article-id pub-id-type="publisher-id">0246</article-id><article-id pub-id-type="doi">10.1128/CDLI.8.5.984-992.2001</article-id><article-categories><subj-group subj-group-type="heading"><subject>Immune-Mediated Responses and Disorders</subject></subj-group></article-categories><title-group><article-title>Presence of Oligoclonal T Cells in Cerebrospinal Fluid of a Child with Multiphasic Disseminated Encephalomyelitis following Hepatitis A Virus Infection</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Oleszak</surname><given-names>Emilia L.</given-names></name><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">1</xref><xref ref-type="author-notes" rid="FN150">*</xref></contrib><contrib contrib-type="author"><name><surname>Lin</surname><given-names>Wan Lu</given-names></name><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">2</xref></contrib><contrib contrib-type="author"><name><surname>Legido</surname><given-names>Agustin</given-names></name><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">3</xref><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">4</xref></contrib><contrib contrib-type="author"><name><surname>Melvin</surname><given-names>Joseph</given-names></name><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">3</xref><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">4</xref></contrib><contrib contrib-type="author"><name><surname>Hardison</surname><given-names>Huntley</given-names></name><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">3</xref><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">4</xref></contrib><contrib contrib-type="author"><name><surname>Hoffman</surname><given-names>Brad E.</given-names></name><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">2</xref></contrib><contrib contrib-type="author"><name><surname>Katsetos</surname><given-names>Christos D.</given-names></name><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">2</xref><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">3</xref><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">4</xref><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">5</xref></contrib><contrib contrib-type="author"><name><surname>Platsoucas</surname><given-names>Chris D.</given-names></name><xref ref-type="aff" rid="N0x9771d58.0x96e5ca0">2</xref></contrib></contrib-group><aff id="N0x9771d58.0x96e5ca0"> Department of Anatomy and Cell Biology, Fels Institute for Cancer Research and Molecular Biology,<sup>1</sup> and Department of Microbiology and Immunology,<sup>2</sup> Temple University School of Medicine, Philadelphia, Pennsylvania 19140; and Department of Pediatrics, MCP Hahnemann University,<sup>3</sup> and Section of Neurology,<sup>4</sup> Department of Pathology and Laboratory Medicine,<sup>5</sup> St. Christopher's Hospital for Children, Philadelphia, Pennsylvania 19134</aff><author-notes><fn id="FN150"><label>*</label><p>Corresponding author. Mailing address: Department of Anatomy and Cell Biology, Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3307 North Broad St., Philadelphia, PA 19140. Phone: (215) 707-7657. Fax: (215) 829-1320. E-mail: <email>eoleszak@astro.temple.edu</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>9</month><year>2001</year></pub-date><volume>8</volume><issue>5</issue><fpage>984</fpage><lpage>992</lpage><history><date date-type="received"><day>20</day><month>11</month><year>2000</year></date><date date-type="rev-request"><day>26</day><month>2</month><year>2001</year></date><date date-type="accepted"><day>22</day><month>6</month><year>2001</year></date></history><copyright-statement>Copyright © 2001, American Society for Microbiology</copyright-statement><copyright-year>2001</copyright-year><abstract><p>We have investigated the clonality of β-chain T-cell receptor (TCR) transcripts from the cerebrospinal fluid (CSF) and peripheral blood from a 7-year old child who developed a multiphasic disseminated encephalomyelitis following an infection with hepatitis A virus. We amplified β-chain TCR transcripts by nonpalindromic adaptor (NPA)-PCR–Vβ-specific PCR. TCR transcripts from only five Vβ families (Vβ13, Vβ3, Vβ17, Vβ8, and Vβ20) were detected in CSF. The amplified products were combined, cloned, and sequenced. Sequence analysis revealed in the CSF substantial proportions of identical β-chain of TCR transcripts, demonstrating oligoclonal populations of T cells. Seventeen of 35 (48%) transcripts were 100% identical, demonstrating a major Vβ13.3 Dβ2.1 Jβ1.3 clonal expansion. Six of 35 (17%) transcripts were also 100% identical, revealing a second Vβ13 clonal expansion (Vβ13.1 Dβ2.1 Jβ1.2). Clonal expansions were also found within the Vβ3 family (transcript Vβ3.1 Dβ2.1 Jβ1.5 accounted for 5 of 35 transcripts [14%]) and within the Vβ20 family (transcript Vβ20.1 Dβ1.1 Jβ2.4 accounted for 3 of 35 transcripts [8%]). These results demonstrate the presence of T-cell oligoclonal expansions in the CSF of this patient following infection with hepatitis A virus. Analysis of the CDR3 motifs revealed that two of the clonally expanded T-cell clones exhibited substantial homology to myelin basic protein-reactive T-cell clones. In contrast, all Vβ TCR families were expressed in peripheral blood lymphocytes. Oligoclonal expansions of T cells were not detected in the peripheral blood of this patient. It remains to be determined whether these clonally expanded T cells are specific for hepatitis A viral antigen(s) or host central nervous system antigen(s) and whether molecular mimicry between hepatitis A viral protein and a host protein is responsible for demyelinating disease in this patient.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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