CovidV2, Pmc, Checkpoint, bibRecord, 000980

Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

Identifieur interne : 000980 ( Pmc/Checkpoint ); précédent : 000979; suivant : 000981

Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

Auteurs : Aleksandar Radoni [Allemagne] ; Stefanie Thulke [Allemagne] ; Hi-Gung Bae [Allemagne] ; Marcel A. Müller [Allemagne] ; Wolfgang Siegert [Allemagne] ; Andreas Nitsche [Allemagne]

Source :

Virology Journal [ 1743-422X ] ; 2005.

RBID : PMC:549079

Abstract

Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC549079

DOI: 10.1186/1743-422X-2-7
PubMed: 15705200
PubMed Central: 549079

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en"><p>Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.</p>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Virol J</journal-id>
<journal-title>Virology Journal</journal-title>
<issn pub-type="epub">1743-422X</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
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<article-meta><article-id pub-id-type="pmid">15705200</article-id>
<article-id pub-id-type="pmc">549079</article-id>
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<article-id pub-id-type="doi">10.1186/1743-422X-2-7</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research</subject>
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<title-group><article-title>Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections</article-title>
</title-group>
<contrib-group><contrib id="A1" corresp="yes" contrib-type="author"><name><surname>Radonić</surname>
<given-names>Aleksandar</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>aleksandar.radonic@charite.de</email>
</contrib>
<contrib id="A2" contrib-type="author"><name><surname>Thulke</surname>
<given-names>Stefanie</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>stefanie.thulke@charite.de</email>
</contrib>
<contrib id="A3" contrib-type="author"><name><surname>Bae</surname>
<given-names>Hi-Gung</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>baeh@rki.de</email>
</contrib>
<contrib id="A4" contrib-type="author"><name><surname>Müller</surname>
<given-names>Marcel A</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>muellerm@rki.de</email>
</contrib>
<contrib id="A5" contrib-type="author"><name><surname>Siegert</surname>
<given-names>Wolfgang</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>wolfgang.siegert@charite.de</email>
</contrib>
<contrib id="A6" contrib-type="author"><name><surname>Nitsche</surname>
<given-names>Andreas</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>nitschea@rki.de</email>
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<aff id="I1"><label>1</label>
Charité – CCM, Medizinische Klinik II m.S. Hämatologie/Onkologie, Berlin, Germany</aff>
<aff id="I2"><label>2</label>
Robert Koch Institut, ZBS 1, Berlin, Germany</aff>
<pub-date pub-type="collection"><year>2005</year>
</pub-date>
<pub-date pub-type="epub"><day>10</day>
<month>2</month>
<year>2005</year>
</pub-date>
<volume>2</volume>
<fpage>7</fpage>
<lpage>7</lpage>
<ext-link ext-link-type="uri" xlink:href="http://www.virologyj.com/content/2/1/7"></ext-link>
<history><date date-type="received"><day>3</day>
<month>2</month>
<year>2005</year>
</date>
<date date-type="accepted"><day>10</day>
<month>2</month>
<year>2005</year>
</date>
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<permissions><copyright-statement>Copyright © 2005 Radonić et al; licensee BioMed Central Ltd.</copyright-statement>
<copyright-year>2005</copyright-year>
<copyright-holder>Radonić et al; licensee BioMed Central Ltd.</copyright-holder>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0"></ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p>
<pmc-comment> 
 Radonić 
 Aleksandar 
  
 aleksandar.radonic@charite.de 
  
 Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections 
 2005Virology Journal 2(1): 7-. (2005)1743-422X(2005)2:1<7>urn:ISSN:1743-422X</pmc-comment>
        </license>
</permissions>
<abstract><p>Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.</p>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Allemagne</li>
</country>
<region><li>Berlin</li>
</region>
<settlement><li>Berlin</li>
</settlement>
</list>
<tree><country name="Allemagne"><region name="Berlin"><name sortKey="Radoni, Aleksandar" sort="Radoni, Aleksandar" uniqKey="Radoni A" first="Aleksandar" last="Radoni">Aleksandar Radoni</name>
</region>
<name sortKey="Bae, Hi Gung" sort="Bae, Hi Gung" uniqKey="Bae H" first="Hi-Gung" last="Bae">Hi-Gung Bae</name>
<name sortKey="Muller, Marcel A" sort="Muller, Marcel A" uniqKey="Muller M" first="Marcel A" last="Müller">Marcel A. Müller</name>
<name sortKey="Nitsche, Andreas" sort="Nitsche, Andreas" uniqKey="Nitsche A" first="Andreas" last="Nitsche">Andreas Nitsche</name>
<name sortKey="Siegert, Wolfgang" sort="Siegert, Wolfgang" uniqKey="Siegert W" first="Wolfgang" last="Siegert">Wolfgang Siegert</name>
<name sortKey="Thulke, Stefanie" sort="Thulke, Stefanie" uniqKey="Thulke S" first="Stefanie" last="Thulke">Stefanie Thulke</name>
</country>
</tree>
</affiliations>
</record>

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Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki