An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes
Identifieur interne : 000277 ( Pmc/Checkpoint ); précédent : 000276; suivant : 000278An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes
Auteurs : Laila Ali Layqah ; Shimaa EissaSource :
- Mikrochimica Acta [ 0026-3672 ] ; 2019.
Abstract
The Middle East respiratory syndrome corona virus (MERS-CoV) is highly pathogenic. An immunosensor for the determination of MERS-CoV is described here. It is based on a competitive assay carried out on an array of carbon electrodes (DEP) modified with gold nanoparticles. Recombinant spike protein S1 was used as a biomarker for MERS CoV. The electrode array enables multiplexed detection of different CoVs. The biosensor is based on indirect competition between free virus in the sample and immobilized MERS-CoV protein for a fixed concentration of antibody added to the sample. Voltammetric response is detected by monitoring the change in the peak current (typically acquired at a working potential of −0.05 V vs. Ag/AgCl) after addition of different concentrations of antigen against MERS-CoV. Electrochemical measurements using ferrocyanide/ferricyanide as a probe were recorded using square wave voltammetry (SWV). Good linear response between the sensor response and the concentrations from 0.001 to 100 ng.mL−1 and 0.01 to 10,000 ng.mL−1 were observed for MERS-CoV and HCoV, respectively. The assay was performed in 20 min with detection limit as low as 0.4 and 1.0 pg.mL−1 for HCoV and MERS-CoV, respectively. The method is highly selective over non-specific proteins such as Influenza A and B. The method is single-step, sensitive and accurate. It was successfully applied to spiked nasal samples.
An electrochemical immunoassay is described for the Middle East Respiratory Syndrome Corona Virus (MERS-CoV). The method is based on a competitive assay carried out on a carbon array electrodes (DEP) nanostructured with gold nanoparticles. The array electrodes enable the multiplexed detection of different types of Corona Virus.
The online version of this article (10.1007/s00604-019-3345-5) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1007/s00604-019-3345-5
PubMed: 30847572
PubMed Central: 7088225
Affiliations:
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<front><div type="abstract" xml:lang="en"><p id="Par1">The Middle East respiratory syndrome corona virus (MERS-CoV) is highly pathogenic. An immunosensor for the determination of MERS-CoV is described here. It is based on a competitive assay carried out on an array of carbon electrodes (DEP) modified with gold nanoparticles. Recombinant spike protein S1 was used as a biomarker for MERS CoV. The electrode array enables multiplexed detection of different CoVs. The biosensor is based on indirect competition between free virus in the sample and immobilized MERS-CoV protein for a fixed concentration of antibody added to the sample. Voltammetric response is detected by monitoring the change in the peak current (typically acquired at a working potential of −0.05 V vs. Ag/AgCl) after addition of different concentrations of antigen against MERS-CoV. Electrochemical measurements using ferrocyanide/ferricyanide as a probe were recorded using square wave voltammetry (SWV). Good linear response between the sensor response and the concentrations from 0.001 to 100 ng.mL<sup>−1</sup>
and 0.01 to 10,000 ng.mL<sup>−1</sup>
were observed for MERS-CoV and HCoV, respectively. The assay was performed in 20 min with detection limit as low as 0.4 and 1.0 pg.mL<sup>−1</sup>
for HCoV and MERS-CoV, respectively. The method is highly selective over non-specific proteins such as Influenza A and B. The method is single-step, sensitive and accurate. It was successfully applied to spiked nasal samples.</p>
<p id="Par2"><fig position="anchor" id="Figa"><label>Graphical abstract</label>
<caption><p>An electrochemical immunoassay is described for the Middle East Respiratory Syndrome Corona Virus (MERS-CoV). The method is based on a competitive assay carried out on a carbon array electrodes (DEP) nanostructured with gold nanoparticles. The array electrodes enable the multiplexed detection of different types of Corona Virus.</p>
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<sec><title>Electronic supplementary material</title>
<p>The online version of this article (10.1007/s00604-019-3345-5) contains supplementary material, which is available to authorized users.</p>
</sec>
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</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Mikrochim Acta</journal-id>
<journal-id journal-id-type="iso-abbrev">Mikrochim Acta</journal-id>
<journal-title-group><journal-title>Mikrochimica Acta</journal-title>
</journal-title-group>
<issn pub-type="ppub">0026-3672</issn>
<issn pub-type="epub">1436-5073</issn>
<publisher><publisher-name>Springer Vienna</publisher-name>
<publisher-loc>Vienna</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">30847572</article-id>
<article-id pub-id-type="pmc">7088225</article-id>
<article-id pub-id-type="publisher-id">3345</article-id>
<article-id pub-id-type="doi">10.1007/s00604-019-3345-5</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Paper</subject>
</subj-group>
</article-categories>
<title-group><article-title>An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Layqah</surname>
<given-names>Laila Ali</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-5558-0060</contrib-id>
<name><surname>Eissa</surname>
<given-names>Shimaa</given-names>
</name>
<address><email>seissa@alfaisal.edu</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1758 7207</institution-id>
<institution-id institution-id-type="GRID">grid.411335.1</institution-id>
<institution>Department of Chemistry,</institution>
<institution>Alfaisal University,</institution>
</institution-wrap>
Al Zahrawi Street, Al Maather, AlTakhassusi Road, Riyadh, 11533 Saudi Arabia</aff>
</contrib-group>
<pub-date pub-type="epub"><day>7</day>
<month>3</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="ppub"><year>2019</year>
</pub-date>
<volume>186</volume>
<issue>4</issue>
<elocation-id>224</elocation-id>
<history><date date-type="received"><day>20</day>
<month>11</month>
<year>2018</year>
</date>
<date date-type="accepted"><day>27</day>
<month>2</month>
<year>2019</year>
</date>
</history>
<permissions><copyright-statement>© Springer-Verlag GmbH Austria, part of Springer Nature 2019</copyright-statement>
<license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p>
</license>
</permissions>
<abstract id="Abs1"><p id="Par1">The Middle East respiratory syndrome corona virus (MERS-CoV) is highly pathogenic. An immunosensor for the determination of MERS-CoV is described here. It is based on a competitive assay carried out on an array of carbon electrodes (DEP) modified with gold nanoparticles. Recombinant spike protein S1 was used as a biomarker for MERS CoV. The electrode array enables multiplexed detection of different CoVs. The biosensor is based on indirect competition between free virus in the sample and immobilized MERS-CoV protein for a fixed concentration of antibody added to the sample. Voltammetric response is detected by monitoring the change in the peak current (typically acquired at a working potential of −0.05 V vs. Ag/AgCl) after addition of different concentrations of antigen against MERS-CoV. Electrochemical measurements using ferrocyanide/ferricyanide as a probe were recorded using square wave voltammetry (SWV). Good linear response between the sensor response and the concentrations from 0.001 to 100 ng.mL<sup>−1</sup>
and 0.01 to 10,000 ng.mL<sup>−1</sup>
were observed for MERS-CoV and HCoV, respectively. The assay was performed in 20 min with detection limit as low as 0.4 and 1.0 pg.mL<sup>−1</sup>
for HCoV and MERS-CoV, respectively. The method is highly selective over non-specific proteins such as Influenza A and B. The method is single-step, sensitive and accurate. It was successfully applied to spiked nasal samples.</p>
<p id="Par2"><fig position="anchor" id="Figa"><label>Graphical abstract</label>
<caption><p>An electrochemical immunoassay is described for the Middle East Respiratory Syndrome Corona Virus (MERS-CoV). The method is based on a competitive assay carried out on a carbon array electrodes (DEP) nanostructured with gold nanoparticles. The array electrodes enable the multiplexed detection of different types of Corona Virus.</p>
</caption>
<graphic position="anchor" xlink:href="604_2019_3345_Figa_HTML" id="MO1"></graphic>
</fig>
</p>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (10.1007/s00604-019-3345-5) contains supplementary material, which is available to authorized users.</p>
</sec>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>Corona virus</kwd>
<kwd>Voltammetry</kwd>
<kwd>Array electrode</kwd>
<kwd>Electrochemical biosensor</kwd>
<kwd>MERS-CoV</kwd>
<kwd>HCoV</kwd>
<kwd>Multiplexed biosensor</kwd>
<kwd>Simultaneous detection</kwd>
<kwd>Competitive immunosensor</kwd>
</kwd-group>
<funding-group><award-group><funding-source><institution>Alfaisal university</institution>
</funding-source>
<award-id>18420</award-id>
<principal-award-recipient><name><surname>Eissa</surname>
<given-names>Shimaa</given-names>
</name>
</principal-award-recipient>
</award-group>
</funding-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© Springer-Verlag GmbH Austria, part of Springer Nature 2019</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Eissa, Shimaa" sort="Eissa, Shimaa" uniqKey="Eissa S" first="Shimaa" last="Eissa">Shimaa Eissa</name>
<name sortKey="Layqah, Laila Ali" sort="Layqah, Laila Ali" uniqKey="Layqah L" first="Laila Ali" last="Layqah">Laila Ali Layqah</name>
</noCountry>
</tree>
</affiliations>
</record>
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