CovidV2, PascalFrancis, Corpus, bibRecord, 000052

Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: Implications for assembly and vaccine production

Identifieur interne : 000052 ( PascalFrancis/Corpus ); précédent : 000051; suivant : 000053

Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: Implications for assembly and vaccine production

Auteurs : YUE HUANG ; Zhi-Yong Yang ; Wing-Pui Kong ; Gary J. Nabel

Source :

Journal of virology [ 0022-538X ] ; 2004.

RBID : Pascal:05-0000571

Descripteurs français

Pascal (Inist)
- Coronavirus, Grave, Malade état grave, Aigu, Voie respiratoire, Vaccin, Prévention, Immunoprophylaxie, Microbiologie, Syndrome respiratoire aigu sévère, Virologie, Forme grave.

English descriptors

KwdEn :
- Acute, Coronavirus, Critically ill, Immunoprophylaxis, Microbiology, Prevention, Respiratory tract, Severe, Severe acute respiratory syndrome, Vaccine, Virology.

Abstract

The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of unknown function. Despite this relative simplicity, the molecular regulation of SARS-CoV replication and assembly is not understood. Here, we report that two viral genes, encoding the SARS-CoV membrane (M) and nucleocapsid (N) proteins, are necessary and sufficient for formation of virus-like particles. Expression vectors encoding these two proteins were synthesized by using preferred human codons. When M and N expression plasmids were cotransfected into human 293 renal epithelial cells, pseudoparticles formed readily. The addition of a third gene, encoding the spike (S) glycoprotein, facilitated budding of particles that contained a corona-like halo resembling SARS-CoV when examined by transmission electron microscopy, with a buoyant density characteristic of coronaviruses. Specific biochemical interactions of these proteins were also shown in vitro. The S, M, and N proteins of the SARS-CoV are, therefore, necessary and sufficient for pseudovirus assembly. These findings advance the understanding of the morphogenesis of SARS-CoV and enable the generation of safe, conformational mimetics of the SARS virus that may facilitate the development of vaccines and antiviral drugs.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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C01	`01`		`ENG`	@0 The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of unknown function. Despite this relative simplicity, the molecular regulation of SARS-CoV replication and assembly is not understood. Here, we report that two viral genes, encoding the SARS-CoV membrane (M) and nucleocapsid (N) proteins, are necessary and sufficient for formation of virus-like particles. Expression vectors encoding these two proteins were synthesized by using preferred human codons. When M and N expression plasmids were cotransfected into human 293 renal epithelial cells, pseudoparticles formed readily. The addition of a third gene, encoding the spike (S) glycoprotein, facilitated budding of particles that contained a corona-like halo resembling SARS-CoV when examined by transmission electron microscopy, with a buoyant density characteristic of coronaviruses. Specific biochemical interactions of these proteins were also shown in vitro. The S, M, and N proteins of the SARS-CoV are, therefore, necessary and sufficient for pseudovirus assembly. These findings advance the understanding of the morphogenesis of SARS-CoV and enable the generation of safe, conformational mimetics of the SARS virus that may facilitate the development of vaccines and antiviral drugs.
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N21				`@1 004`
N44	`01`			`@1 OTO`
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Format Inist (serveur)

NO :	PASCAL 05-0000571 INIST
ET :	Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: Implications for assembly and vaccine production
AU :	YUE HUANG; YANG (Zhi-Yong); KONG (Wing-Pui); NABEL (Gary J.)
AF :	Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health/Bethesda, Maryland/Etats-Unis (1 aut., 2 aut., 3 aut., 4 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2004; Vol. 78; No. 22; Pp. 12557-12565; Bibl. 35 ref.
LA :	Anglais
EA :	The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of unknown function. Despite this relative simplicity, the molecular regulation of SARS-CoV replication and assembly is not understood. Here, we report that two viral genes, encoding the SARS-CoV membrane (M) and nucleocapsid (N) proteins, are necessary and sufficient for formation of virus-like particles. Expression vectors encoding these two proteins were synthesized by using preferred human codons. When M and N expression plasmids were cotransfected into human 293 renal epithelial cells, pseudoparticles formed readily. The addition of a third gene, encoding the spike (S) glycoprotein, facilitated budding of particles that contained a corona-like halo resembling SARS-CoV when examined by transmission electron microscopy, with a buoyant density characteristic of coronaviruses. Specific biochemical interactions of these proteins were also shown in vitro. The S, M, and N proteins of the SARS-CoV are, therefore, necessary and sufficient for pseudovirus assembly. These findings advance the understanding of the morphogenesis of SARS-CoV and enable the generation of safe, conformational mimetics of the SARS virus that may facilitate the development of vaccines and antiviral drugs.
CC :	002A05C10; 002A05C07
FD :	Coronavirus; Grave; Malade état grave; Aigu; Voie respiratoire; Vaccin; Prévention; Immunoprophylaxie; Microbiologie; Syndrome respiratoire aigu sévère; Virologie; Forme grave
FG :	Coronaviridae; Nidovirales; Virus; Virose; Infection; Appareil respiratoire pathologie; Poumon pathologie; Appareil respiratoire
ED :	Coronavirus; Severe; Critically ill; Acute; Respiratory tract; Vaccine; Prevention; Immunoprophylaxis; Microbiology; Severe acute respiratory syndrome; Virology
EG :	Coronaviridae; Nidovirales; Virus; Viral disease; Infection; Respiratory disease; Lung disease; Respiratory system
SD :	Coronavirus; Grave; Enfermo estado grave; Agudo; Vía respiratoria; Vacuna; Prevención; Inmunoprofilaxia; Microbiología; Síndrome respiratorio agudo severo; Virología
LO :	INIST-13592.354000122578960480
ID :	05-0000571

Links to Exploration step

Pascal:05-0000571

Le document en format XML

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<server><NO>PASCAL 05-0000571 INIST</NO>
<ET>Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: Implications for assembly and vaccine production</ET>
<AU>YUE HUANG; YANG (Zhi-Yong); KONG (Wing-Pui); NABEL (Gary J.)</AU>
<AF>Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health/Bethesda, Maryland/Etats-Unis (1 aut., 2 aut., 3 aut., 4 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of virology; ISSN 0022-538X; Etats-Unis; Da. 2004; Vol. 78; No. 22; Pp. 12557-12565; Bibl. 35 ref.</SO>
<LA>Anglais</LA>
<EA>The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of unknown function. Despite this relative simplicity, the molecular regulation of SARS-CoV replication and assembly is not understood. Here, we report that two viral genes, encoding the SARS-CoV membrane (M) and nucleocapsid (N) proteins, are necessary and sufficient for formation of virus-like particles. Expression vectors encoding these two proteins were synthesized by using preferred human codons. When M and N expression plasmids were cotransfected into human 293 renal epithelial cells, pseudoparticles formed readily. The addition of a third gene, encoding the spike (S) glycoprotein, facilitated budding of particles that contained a corona-like halo resembling SARS-CoV when examined by transmission electron microscopy, with a buoyant density characteristic of coronaviruses. Specific biochemical interactions of these proteins were also shown in vitro. The S, M, and N proteins of the SARS-CoV are, therefore, necessary and sufficient for pseudovirus assembly. These findings advance the understanding of the morphogenesis of SARS-CoV and enable the generation of safe, conformational mimetics of the SARS virus that may facilitate the development of vaccines and antiviral drugs.</EA>
<CC>002A05C10; 002A05C07</CC>
<FD>Coronavirus; Grave; Malade état grave; Aigu; Voie respiratoire; Vaccin; Prévention; Immunoprophylaxie; Microbiologie; Syndrome respiratoire aigu sévère; Virologie; Forme grave</FD>
<FG>Coronaviridae; Nidovirales; Virus; Virose; Infection; Appareil respiratoire pathologie; Poumon pathologie; Appareil respiratoire</FG>
<ED>Coronavirus; Severe; Critically ill; Acute; Respiratory tract; Vaccine; Prevention; Immunoprophylaxis; Microbiology; Severe acute respiratory syndrome; Virology</ED>
<EG>Coronaviridae; Nidovirales; Virus; Viral disease; Infection; Respiratory disease; Lung disease; Respiratory system</EG>
<SD>Coronavirus; Grave; Enfermo estado grave; Agudo; Vía respiratoria; Vacuna; Prevención; Inmunoprofilaxia; Microbiología; Síndrome respiratorio agudo severo; Virología</SD>
<LO>INIST-13592.354000122578960480</LO>
<ID>05-0000571</ID>
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Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: Implications for assembly and vaccine production

Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: Implications for assembly and vaccine production

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