Conserved antigenic sites between MERS-CoV and Bat-coronavirus are revealed through sequence analysis
Identifieur interne : 000597 ( Ncbi/Merge ); précédent : 000596; suivant : 000598Conserved antigenic sites between MERS-CoV and Bat-coronavirus are revealed through sequence analysis
Auteurs : Refat Sharmin ; Abul B. M. M. K. Islam [Bangladesh]Source :
- Source Code for Biology and Medicine [ 1751-0473 ] ; 2016.
Abstract
MERS-CoV is a newly emerged human coronavirus reported closely related with HKU4 and HKU5 Bat coronaviruses. Bat and MERS corona-viruses are structurally related. Therefore, it is of interest to estimate the degree of conserved antigenic sites among them. It is of importance to elucidate the shared antigenic-sites and extent of conservation between them to understand the evolutionary dynamics of MERS-CoV.
Multiple sequence alignment of the spike (S), membrane (M), enveloped (E) and nucleocapsid (N) proteins was employed to identify the sequence conservation among MERS and Bat (HKU4, HKU5) coronaviruses. We used various
This sharing suggests that in case of pathogenicity MERS-CoV is more closely related to HKU5 bat-CoV than HKU4 bat-CoV. The conserved epitopes indicates their evolutionary relationship and ancestry of pathogenicity.
The online version of this article (doi:10.1186/s13029-016-0049-7) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s13029-016-0049-7
PubMed: 26962326
PubMed Central: 4784407
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<author><name sortKey="Sharmin, Refat" sort="Sharmin, Refat" uniqKey="Sharmin R" first="Refat" last="Sharmin">Refat Sharmin</name>
<affiliation><nlm:aff id="Aff1">Research and Development Department, Incepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341 Bangladesh</nlm:aff>
<wicri:noCountry code="subfield">Dhaka 1341 Bangladesh</wicri:noCountry>
</affiliation>
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<author><name sortKey="Islam, Abul B M M K" sort="Islam, Abul B M M K" uniqKey="Islam A" first="Abul B. M. M. K." last="Islam">Abul B. M. M. K. Islam</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Genetic Engineering and Biotechnology, University of Dhaka, Science Complex Building, Dhaka, 1000 Bangladesh</nlm:aff>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Conserved antigenic sites between MERS-CoV and Bat-coronavirus are revealed through sequence analysis</title>
<author><name sortKey="Sharmin, Refat" sort="Sharmin, Refat" uniqKey="Sharmin R" first="Refat" last="Sharmin">Refat Sharmin</name>
<affiliation><nlm:aff id="Aff1">Research and Development Department, Incepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341 Bangladesh</nlm:aff>
<wicri:noCountry code="subfield">Dhaka 1341 Bangladesh</wicri:noCountry>
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<author><name sortKey="Islam, Abul B M M K" sort="Islam, Abul B M M K" uniqKey="Islam A" first="Abul B. M. M. K." last="Islam">Abul B. M. M. K. Islam</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Genetic Engineering and Biotechnology, University of Dhaka, Science Complex Building, Dhaka, 1000 Bangladesh</nlm:aff>
<country xml:lang="fr">Bangladesh</country>
<wicri:regionArea>Department of Genetic Engineering and Biotechnology, University of Dhaka, Science Complex Building, Dhaka</wicri:regionArea>
<wicri:noRegion>Dhaka</wicri:noRegion>
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<series><title level="j">Source Code for Biology and Medicine</title>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>MERS-CoV is a newly emerged human coronavirus reported closely related with HKU4 and HKU5 Bat coronaviruses. Bat and MERS corona-viruses are structurally related. Therefore, it is of interest to estimate the degree of conserved antigenic sites among them. It is of importance to elucidate the shared antigenic-sites and extent of conservation between them to understand the evolutionary dynamics of MERS-CoV.</p>
</sec>
<sec><title>Results</title>
<p>Multiple sequence alignment of the spike (S), membrane (M), enveloped (E) and nucleocapsid (N) proteins was employed to identify the sequence conservation among MERS and Bat (HKU4, HKU5) coronaviruses. We used various <italic>in silico</italic>
tools to predict the conserved antigenic sites. We found that MERS-CoV shared 30 % of its S protein antigenic sites with HKU4 and 70 % with HKU5 bat-CoV. Whereas 100 % of its E, M and N protein’s antigenic sites are found to be conserved with those in HKU4 and HKU5.</p>
</sec>
<sec><title>Conclusion</title>
<p>This sharing suggests that in case of pathogenicity MERS-CoV is more closely related to HKU5 bat-CoV than HKU4 bat-CoV. The conserved epitopes indicates their evolutionary relationship and ancestry of pathogenicity.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s13029-016-0049-7) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Source Code Biol Med</journal-id>
<journal-id journal-id-type="iso-abbrev">Source Code Biol Med</journal-id>
<journal-title-group><journal-title>Source Code for Biology and Medicine</journal-title>
</journal-title-group>
<issn pub-type="epub">1751-0473</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">26962326</article-id>
<article-id pub-id-type="pmc">4784407</article-id>
<article-id pub-id-type="publisher-id">49</article-id>
<article-id pub-id-type="doi">10.1186/s13029-016-0049-7</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research</subject>
</subj-group>
</article-categories>
<title-group><article-title>Conserved antigenic sites between MERS-CoV and Bat-coronavirus are revealed through sequence analysis</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Sharmin</surname>
<given-names>Refat</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Islam</surname>
<given-names>Abul B. M. M. K.</given-names>
</name>
<address><phone>+880-2-9661900</phone>
<email>khademul@du.ac.bd</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<aff id="Aff1"><label></label>
Research and Development Department, Incepta Vaccine Ltd., Zirabo, Savar, Dhaka 1341 Bangladesh</aff>
<aff id="Aff2"><label></label>
Department of Genetic Engineering and Biotechnology, University of Dhaka, Science Complex Building, Dhaka, 1000 Bangladesh</aff>
</contrib-group>
<pub-date pub-type="epub"><day>9</day>
<month>3</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>9</day>
<month>3</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="collection"><year>2016</year>
</pub-date>
<volume>11</volume>
<elocation-id>3</elocation-id>
<history><date date-type="received"><day>21</day>
<month>5</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>26</day>
<month>2</month>
<year>2016</year>
</date>
</history>
<permissions><copyright-statement>© Sharmin and Islam. 2016</copyright-statement>
<license license-type="OpenAccess"><license-p><bold>Open Access</bold>
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>
) applies to the data made available in this article, unless otherwise stated.</license-p>
</license>
</permissions>
<abstract id="Abs1"><sec><title>Background</title>
<p>MERS-CoV is a newly emerged human coronavirus reported closely related with HKU4 and HKU5 Bat coronaviruses. Bat and MERS corona-viruses are structurally related. Therefore, it is of interest to estimate the degree of conserved antigenic sites among them. It is of importance to elucidate the shared antigenic-sites and extent of conservation between them to understand the evolutionary dynamics of MERS-CoV.</p>
</sec>
<sec><title>Results</title>
<p>Multiple sequence alignment of the spike (S), membrane (M), enveloped (E) and nucleocapsid (N) proteins was employed to identify the sequence conservation among MERS and Bat (HKU4, HKU5) coronaviruses. We used various <italic>in silico</italic>
tools to predict the conserved antigenic sites. We found that MERS-CoV shared 30 % of its S protein antigenic sites with HKU4 and 70 % with HKU5 bat-CoV. Whereas 100 % of its E, M and N protein’s antigenic sites are found to be conserved with those in HKU4 and HKU5.</p>
</sec>
<sec><title>Conclusion</title>
<p>This sharing suggests that in case of pathogenicity MERS-CoV is more closely related to HKU5 bat-CoV than HKU4 bat-CoV. The conserved epitopes indicates their evolutionary relationship and ancestry of pathogenicity.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s13029-016-0049-7) contains supplementary material, which is available to authorized users.</p>
</sec>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>MERS-CoV</kwd>
<kwd>HKU4</kwd>
<kwd>HKU5</kwd>
<kwd>Epitope</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2016</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Bangladesh</li>
</country>
</list>
<tree><noCountry><name sortKey="Sharmin, Refat" sort="Sharmin, Refat" uniqKey="Sharmin R" first="Refat" last="Sharmin">Refat Sharmin</name>
</noCountry>
<country name="Bangladesh"><noRegion><name sortKey="Islam, Abul B M M K" sort="Islam, Abul B M M K" uniqKey="Islam A" first="Abul B. M. M. K." last="Islam">Abul B. M. M. K. Islam</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
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