Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses
Identifieur interne : 000129 ( Ncbi/Merge ); précédent : 000128; suivant : 000130Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses
Auteurs : Sarah Watson [Australie] ; Sarah Mercier [Australie] ; Chris Bye [Australie] ; John Wilkinson [Australie] ; Anthony L. Cunningham [Australie] ; Andrew N. Harman [Australie]Source :
- Virology Journal [ 1743-422X ] ; 2007.
Abstract
The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in the context of viral infections as viruses can significantly interfere with host cell pathways, the components of which traditional housekeeping genes often encode. In this study we have determined the reliability of 10 housekeeping genes in context of four heavily studied viral infections; human immunodeficiency virus type 1, herpes simplex virus type 1, cytomegalovirus and varicella zoster virus infections using a variety of cell types and virus strains. This provides researchers of these viruses with a shortlist of potential housekeeping genes to use as normalisers for QPCR experiments.
Url:
DOI: 10.1186/1743-422X-4-130
PubMed: 18053162
PubMed Central: 2216015
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<front><div type="abstract" xml:lang="en"><p>The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in the context of viral infections as viruses can significantly interfere with host cell pathways, the components of which traditional housekeeping genes often encode. In this study we have determined the reliability of 10 housekeeping genes in context of four heavily studied viral infections; human immunodeficiency virus type 1, herpes simplex virus type 1, cytomegalovirus and varicella zoster virus infections using a variety of cell types and virus strains. This provides researchers of these viruses with a shortlist of potential housekeeping genes to use as normalisers for QPCR experiments.</p>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Virol J</journal-id>
<journal-title>Virology Journal</journal-title>
<issn pub-type="epub">1743-422X</issn>
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<article-categories><subj-group subj-group-type="heading"><subject>Methodology</subject>
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<title-group><article-title>Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses</article-title>
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<contrib-group><contrib id="A1" contrib-type="author"><name><surname>Watson</surname>
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<email>sarahlouise53@hotmail.com</email>
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<given-names>Sarah</given-names>
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<email>john_wilkinson@cfi.unsw.edu.au</email>
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<contrib id="A5" contrib-type="author"><name><surname>Cunningham</surname>
<given-names>Anthony L</given-names>
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<email>tony_cunningham@wmi.usyd.edu.au</email>
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<aff id="I1"><label>1</label>
Centre for Virus Research, Westmead Millennium Institute, Sydney, Australia</aff>
<aff id="I2"><label>2</label>
The Howard Florey Institute, University of Melbourne, Melbourne, Australia</aff>
<aff id="I3"><label>3</label>
Biotron Limited, Centre for Immunology, St. Vincent's Hospital, Sydney, Australia</aff>
<pub-date pub-type="collection"><year>2007</year>
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<history><date date-type="received"><day>10</day>
<month>10</month>
<year>2007</year>
</date>
<date date-type="accepted"><day>3</day>
<month>12</month>
<year>2007</year>
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<permissions><copyright-statement>Copyright © 2007 Watson et al; licensee BioMed Central Ltd.</copyright-statement>
<copyright-year>2007</copyright-year>
<copyright-holder>Watson et al; licensee BioMed Central Ltd.</copyright-holder>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0"></ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p>
<pmc-comment>
Watson
Sarah
sarahlouise53@hotmail.com
Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses
2007 Virology Journal 4(1): 130-. (2007) 1743-422X(2007)4:1<130> urn:ISSN:1743-422X </pmc-comment>
</license>
</permissions>
<abstract><p>The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in the context of viral infections as viruses can significantly interfere with host cell pathways, the components of which traditional housekeeping genes often encode. In this study we have determined the reliability of 10 housekeeping genes in context of four heavily studied viral infections; human immunodeficiency virus type 1, herpes simplex virus type 1, cytomegalovirus and varicella zoster virus infections using a variety of cell types and virus strains. This provides researchers of these viruses with a shortlist of potential housekeeping genes to use as normalisers for QPCR experiments.</p>
</abstract>
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<affiliations><list><country><li>Australie</li>
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