A comparison between immunofluorescence staining on smears from Membrana nictitans (M3 test), immunohistopathology and routine pathology in cats with suspected feline infectious peritonitis (FIP).
Identifieur interne : 000090 ( Ncbi/Merge ); précédent : 000089; suivant : 000091A comparison between immunofluorescence staining on smears from Membrana nictitans (M3 test), immunohistopathology and routine pathology in cats with suspected feline infectious peritonitis (FIP).
Auteurs : K. Hök [Suède]Source :
- Acta veterinaria Scandinavica [ 0044-605X ] ; 1991.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Antigènes viraux.
- anatomopathologie : Péritonite infectieuse féline.
- diagnostic : Péritonite infectieuse féline.
- immunologie : Coronavirus félin.
- microbiologie : Membrane nictitante.
- Animaux, Chats, Technique d'immunofluorescence, Valeur prédictive des tests.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antigens, Viral.
- diagnosis : Feline Infectious Peritonitis.
- immunology : Coronavirus, Feline.
- microbiology : Nictitating Membrane.
- pathology : Feline Infectious Peritonitis.
- Animals, Cats, Fluorescent Antibody Technique, Predictive Value of Tests.
Abstract
An indirect immunofluorescence method using smears from membrana nictitans (M3 test) to diagnose feline corona virus (FCV) infection was compared with immunohistopathology (using indirect immunofluorescence assay (IFFA) performed on organs (IFO], and routine pathology (RP) in cats with suspected feline infectious peritonitis (FIP). A close correlation between the 2 immunofluorescence methods (IFO and M3) was observed. Although the M3 test requires samples from only 1 organ per animal, both the sensitivity and specificity were high (80%), when compared to IFO (using samples from an average of 5 organs per animal). In 21% of the cats with suspected FIP typical pathological lesions were found. As the M3 test is relatively easy to perform, it could reduce work-load of pathology laboratories and provide valuable data for clinical and epidemiological use.
PubMed: 1666490
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pubmed:1666490Le document en format XML
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<author><name sortKey="Hok, K" sort="Hok, K" uniqKey="Hok K" first="K" last="Hök">K. Hök</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Medical Microbial Ecology, Karolinska Institute, Stockholm, Sweden.</nlm:affiliation>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">A comparison between immunofluorescence staining on smears from Membrana nictitans (M3 test), immunohistopathology and routine pathology in cats with suspected feline infectious peritonitis (FIP).</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antigens, Viral (analysis)</term>
<term>Cats</term>
<term>Coronavirus, Feline (immunology)</term>
<term>Feline Infectious Peritonitis (diagnosis)</term>
<term>Feline Infectious Peritonitis (pathology)</term>
<term>Fluorescent Antibody Technique</term>
<term>Nictitating Membrane (microbiology)</term>
<term>Predictive Value of Tests</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Antigènes viraux (analyse)</term>
<term>Chats</term>
<term>Coronavirus félin (immunologie)</term>
<term>Membrane nictitante (microbiologie)</term>
<term>Péritonite infectieuse féline (anatomopathologie)</term>
<term>Péritonite infectieuse féline (diagnostic)</term>
<term>Technique d'immunofluorescence</term>
<term>Valeur prédictive des tests</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term>
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<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Péritonite infectieuse féline</term>
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<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Péritonite infectieuse féline</term>
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<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus félin</term>
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<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Membrane nictitante</term>
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<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Nictitating Membrane</term>
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<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Feline Infectious Peritonitis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cats</term>
<term>Fluorescent Antibody Technique</term>
<term>Predictive Value of Tests</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Chats</term>
<term>Technique d'immunofluorescence</term>
<term>Valeur prédictive des tests</term>
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<front><div type="abstract" xml:lang="en">An indirect immunofluorescence method using smears from membrana nictitans (M3 test) to diagnose feline corona virus (FCV) infection was compared with immunohistopathology (using indirect immunofluorescence assay (IFFA) performed on organs (IFO], and routine pathology (RP) in cats with suspected feline infectious peritonitis (FIP). A close correlation between the 2 immunofluorescence methods (IFO and M3) was observed. Although the M3 test requires samples from only 1 organ per animal, both the sensitivity and specificity were high (80%), when compared to IFO (using samples from an average of 5 organs per animal). In 21% of the cats with suspected FIP typical pathological lesions were found. As the M3 test is relatively easy to perform, it could reduce work-load of pathology laboratories and provide valuable data for clinical and epidemiological use.</div>
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<ArticleTitle>A comparison between immunofluorescence staining on smears from Membrana nictitans (M3 test), immunohistopathology and routine pathology in cats with suspected feline infectious peritonitis (FIP).</ArticleTitle>
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<Abstract><AbstractText>An indirect immunofluorescence method using smears from membrana nictitans (M3 test) to diagnose feline corona virus (FCV) infection was compared with immunohistopathology (using indirect immunofluorescence assay (IFFA) performed on organs (IFO], and routine pathology (RP) in cats with suspected feline infectious peritonitis (FIP). A close correlation between the 2 immunofluorescence methods (IFO and M3) was observed. Although the M3 test requires samples from only 1 organ per animal, both the sensitivity and specificity were high (80%), when compared to IFO (using samples from an average of 5 organs per animal). In 21% of the cats with suspected FIP typical pathological lesions were found. As the M3 test is relatively easy to perform, it could reduce work-load of pathology laboratories and provide valuable data for clinical and epidemiological use.</AbstractText>
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