Characterization of the Coronavirus M Protein and Nucleocapsid Interaction in Infected Cells
Identifieur interne : 000006 ( Ncbi/Merge ); précédent : 000005; suivant : 000007Characterization of the Coronavirus M Protein and Nucleocapsid Interaction in Infected Cells
Auteurs : Krishna Narayanan ; Akihiko Maeda ; Junko Maeda ; Shinji MakinoSource :
- Journal of Virology [ 0022-538X ] ; 2000.
Abstract
Coronavirus contains three envelope proteins, M, E and S, and a nucleocapsid, which consists of genomic RNA and N protein, within the viral envelope. We studied the macromolecular interactions involved in coronavirus assembly in cells infected with a murine coronavirus, mouse hepatitis virus (MHV). Coimmunoprecipitation analyses demonstrated an interaction between N protein and M protein in infected cells. Pulse-labeling experiments showed that newly synthesized, unglycosylated M protein interacted with N protein in a pre-Golgi compartment, which is part of the MHV budding site. Coimmunoprecipitation analyses further revealed that M protein interacted with only genomic-length MHV mRNA, mRNA 1, while N protein interacted with all MHV mRNAs. These data indicated that M protein interacted with the nucleocapsid, consisting of N protein and mRNA 1, in infected cells. The M protein-nucleocapsid interaction occurred in the absence of S and E proteins. Intracellular M protein-N protein interaction was maintained after removal of viral RNAs by RNase treatment. However, the M protein-N protein interaction did not occur in cells coexpressing M protein and N protein alone. These data indicated that while the M protein-N protein interaction, which is independent of viral RNA, occurred in the M protein-nucleocapsid complex, some MHV function(s) was necessary for the initiation of M protein-nucleocapsid interaction. The M protein-nucleocapsid interaction, which occurred near or at the MHV budding site, most probably represented the process of specific packaging of the MHV genome into MHV particles.
Url:
PubMed: 10933723
PubMed Central: 112346
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000151
- to stream Pmc, to step Curation: 000151
- to stream Pmc, to step Checkpoint: 000A27
Links to Exploration step
PMC:112346Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of the Coronavirus M Protein and Nucleocapsid Interaction in Infected Cells</title>
<author><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name>
</author>
<author><name sortKey="Maeda, Akihiko" sort="Maeda, Akihiko" uniqKey="Maeda A" first="Akihiko" last="Maeda">Akihiko Maeda</name>
</author>
<author><name sortKey="Maeda, Junko" sort="Maeda, Junko" uniqKey="Maeda J" first="Junko" last="Maeda">Junko Maeda</name>
</author>
<author><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">10933723</idno>
<idno type="pmc">112346</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC112346</idno>
<idno type="RBID">PMC:112346</idno>
<date when="2000">2000</date>
<idno type="wicri:Area/Pmc/Corpus">000151</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000151</idno>
<idno type="wicri:Area/Pmc/Curation">000151</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000151</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000A27</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A27</idno>
<idno type="wicri:Area/Ncbi/Merge">000006</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Characterization of the Coronavirus M Protein and Nucleocapsid Interaction in Infected Cells</title>
<author><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name>
</author>
<author><name sortKey="Maeda, Akihiko" sort="Maeda, Akihiko" uniqKey="Maeda A" first="Akihiko" last="Maeda">Akihiko Maeda</name>
</author>
<author><name sortKey="Maeda, Junko" sort="Maeda, Junko" uniqKey="Maeda J" first="Junko" last="Maeda">Junko Maeda</name>
</author>
<author><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2000">2000</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Coronavirus contains three envelope proteins, M, E and S, and a nucleocapsid, which consists of genomic RNA and N protein, within the viral envelope. We studied the macromolecular interactions involved in coronavirus assembly in cells infected with a murine coronavirus, mouse hepatitis virus (MHV). Coimmunoprecipitation analyses demonstrated an interaction between N protein and M protein in infected cells. Pulse-labeling experiments showed that newly synthesized, unglycosylated M protein interacted with N protein in a pre-Golgi compartment, which is part of the MHV budding site. Coimmunoprecipitation analyses further revealed that M protein interacted with only genomic-length MHV mRNA, mRNA 1, while N protein interacted with all MHV mRNAs. These data indicated that M protein interacted with the nucleocapsid, consisting of N protein and mRNA 1, in infected cells. The M protein-nucleocapsid interaction occurred in the absence of S and E proteins. Intracellular M protein-N protein interaction was maintained after removal of viral RNAs by RNase treatment. However, the M protein-N protein interaction did not occur in cells coexpressing M protein and N protein alone. These data indicated that while the M protein-N protein interaction, which is independent of viral RNA, occurred in the M protein-nucleocapsid complex, some MHV function(s) was necessary for the initiation of M protein-nucleocapsid interaction. The M protein-nucleocapsid interaction, which occurred near or at the MHV budding site, most probably represented the process of specific packaging of the MHV genome into MHV particles.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Virol</journal-id>
<journal-id journal-id-type="publisher-id">J VIROL</journal-id>
<journal-title>Journal of Virology</journal-title>
<issn pub-type="ppub">0022-538X</issn>
<issn pub-type="epub">1098-5514</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">10933723</article-id>
<article-id pub-id-type="pmc">112346</article-id>
<article-id pub-id-type="publisher-id">0566</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Structure and Assembly</subject>
</subj-group>
</article-categories>
<title-group><article-title>Characterization of the Coronavirus M Protein and Nucleocapsid Interaction in Infected Cells</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Narayanan</surname>
<given-names>Krishna</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Maeda</surname>
<given-names>Akihiko</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Maeda</surname>
<given-names>Junko</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Makino</surname>
<given-names>Shinji</given-names>
</name>
<xref ref-type="author-notes" rid="FN150">*</xref>
</contrib>
</contrib-group>
<aff id="N0x9ba6868.0x9c37c20">Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1019, and Department of Microbiology and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712-1095</aff>
<author-notes><fn id="FN150"><label>*</label>
<p>Corresponding author. Mailing address: Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555-1019. Phone: (409) 772-2323. Fax: (409) 772-5065. E-mail: <email>shmakino@utmb.edu</email>
.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>9</month>
<year>2000</year>
</pub-date>
<volume>74</volume>
<issue>17</issue>
<fpage>8127</fpage>
<lpage>8134</lpage>
<history><date date-type="received"><day>30</day>
<month>3</month>
<year>2000</year>
</date>
<date date-type="accepted"><day>8</day>
<month>6</month>
<year>2000</year>
</date>
</history>
<copyright-statement>Copyright © 2000, American Society for Microbiology</copyright-statement>
<copyright-year>2000</copyright-year>
<abstract><p>Coronavirus contains three envelope proteins, M, E and S, and a nucleocapsid, which consists of genomic RNA and N protein, within the viral envelope. We studied the macromolecular interactions involved in coronavirus assembly in cells infected with a murine coronavirus, mouse hepatitis virus (MHV). Coimmunoprecipitation analyses demonstrated an interaction between N protein and M protein in infected cells. Pulse-labeling experiments showed that newly synthesized, unglycosylated M protein interacted with N protein in a pre-Golgi compartment, which is part of the MHV budding site. Coimmunoprecipitation analyses further revealed that M protein interacted with only genomic-length MHV mRNA, mRNA 1, while N protein interacted with all MHV mRNAs. These data indicated that M protein interacted with the nucleocapsid, consisting of N protein and mRNA 1, in infected cells. The M protein-nucleocapsid interaction occurred in the absence of S and E proteins. Intracellular M protein-N protein interaction was maintained after removal of viral RNAs by RNase treatment. However, the M protein-N protein interaction did not occur in cells coexpressing M protein and N protein alone. These data indicated that while the M protein-N protein interaction, which is independent of viral RNA, occurred in the M protein-nucleocapsid complex, some MHV function(s) was necessary for the initiation of M protein-nucleocapsid interaction. The M protein-nucleocapsid interaction, which occurred near or at the MHV budding site, most probably represented the process of specific packaging of the MHV genome into MHV particles.</p>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Maeda, Akihiko" sort="Maeda, Akihiko" uniqKey="Maeda A" first="Akihiko" last="Maeda">Akihiko Maeda</name>
<name sortKey="Maeda, Junko" sort="Maeda, Junko" uniqKey="Maeda J" first="Junko" last="Maeda">Junko Maeda</name>
<name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name>
<name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000006 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000006 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV2 |flux= Ncbi |étape= Merge |type= RBID |clé= PMC:112346 |texte= Characterization of the Coronavirus M Protein and Nucleocapsid Interaction in Infected Cells }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:10933723" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a CovidV2
This area was generated with Dilib version V0.6.33. |