Replication of two porcine parvovirus isolates at non-permissive temperatures
Identifieur interne : 001C41 ( Main/Merge ); précédent : 001C40; suivant : 001C42Replication of two porcine parvovirus isolates at non-permissive temperatures
Auteurs : C. S. Choi [États-Unis] ; H. S. Joo [États-Unis] ; T. W. Molitor [États-Unis]Source :
- Archives of Virology [ 0304-8608 ] ; 1990-09-01.
English descriptors
- Teeft :
- Arch virol, Choi, Empty particles, Fetal death, Fetus, Fetuses inoculated, Infectious virus, Infectious virus titers, Inoculated, Kbsh, Kresse, Molitor, Mutant, Parvovirus, Passaged, Pathogenicity, Polypeptide, Porcine, Porcine parvovirus, Porcine parvovirus replication, Previous studies, Pseudorabies virus, Replication, Serial passage, Serial passages, Serially passaged, Stock virus, Swine, Swine fetuses, Times passage, Viral, Viral antigen, Viral polypeptides, Virol, Virus, Virus preparation, Virus replication.
Abstract
Summary: Previous studies have shown that replication in vitro of the porcine parvovirus (PPV) isolate, KBSH, was restricted at 39°C but not at 37°C. In contrast, replication of the Kresse isolate was restricted at 37°C but not at 39°C. In this study, Kresse and KBSH isolates were passaged up to ten times in swine testicle (ST) cells at non-permissive temperatures, and at subsequent passage viral protein synthesis, viral DNA synthesis, and progeny virus were evaluated. KBSH became adapted for replication at 39°C upon serial passages, displaying an appreciable increase in viral progeny, viral polypeptides, and viral DNA concentration. This finding was also observed with Kresse virus isolate continuously passaged at 37°C. Neither isolate became adapted for replication at 32°C. In an attempt to examine the effect of in vitro passage at non-permissive temperatures on pathogenicity in swine, KBSH passaged 10 times either at 37°C or 39°C was inoculated into swine fetuses. Two of four fetuses inoculated with 39°C-passaged KBSH were dead and hemorrhagic or mummified. All four fetuses inoculated with 39°C-KBSH contained viral antigen and viral DNA. In contrast, fetuses inoculated with 37°C-passaged KBSH, or with cell culture fluid were normal in appearance. Viral antigen and viral DNA were not demonstrated in fetuses inoculated with 37°C-KBSH or cell culture fluids. These findings suggest the possibility that the ability to replicate at 39°C is associated with virulence in swine fetuses.
Url:
DOI: 10.1007/BF01316676
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<front><div type="abstract" xml:lang="en">Summary: Previous studies have shown that replication in vitro of the porcine parvovirus (PPV) isolate, KBSH, was restricted at 39°C but not at 37°C. In contrast, replication of the Kresse isolate was restricted at 37°C but not at 39°C. In this study, Kresse and KBSH isolates were passaged up to ten times in swine testicle (ST) cells at non-permissive temperatures, and at subsequent passage viral protein synthesis, viral DNA synthesis, and progeny virus were evaluated. KBSH became adapted for replication at 39°C upon serial passages, displaying an appreciable increase in viral progeny, viral polypeptides, and viral DNA concentration. This finding was also observed with Kresse virus isolate continuously passaged at 37°C. Neither isolate became adapted for replication at 32°C. In an attempt to examine the effect of in vitro passage at non-permissive temperatures on pathogenicity in swine, KBSH passaged 10 times either at 37°C or 39°C was inoculated into swine fetuses. Two of four fetuses inoculated with 39°C-passaged KBSH were dead and hemorrhagic or mummified. All four fetuses inoculated with 39°C-KBSH contained viral antigen and viral DNA. In contrast, fetuses inoculated with 37°C-passaged KBSH, or with cell culture fluid were normal in appearance. Viral antigen and viral DNA were not demonstrated in fetuses inoculated with 37°C-KBSH or cell culture fluids. These findings suggest the possibility that the ability to replicate at 39°C is associated with virulence in swine fetuses.</div>
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