Rabbit sucrase-isomaltase contains a functional intestinal receptor for Clostridium difficile toxin A.
Identifieur interne : 001A14 ( Main/Merge ); précédent : 001A13; suivant : 001A15Rabbit sucrase-isomaltase contains a functional intestinal receptor for Clostridium difficile toxin A.
Auteurs : C. Pothoulakis ; R J Gilbert ; C. Cladaras ; I. Castagliuolo ; G. Semenza ; Y. Hitti ; J S Montcrief ; J. Linevsky ; C P Kelly ; S. Nikulasson ; H P Desai ; T D Wilkins ; J T LamontSource :
- Journal of Clinical Investigation [ 0021-9738 ] ; 1996.
Abstract
The intestinal effects of Clostridium difficile toxin A are inidated by toxin binding to luminal enterocyte receptors. We reported previously that the rabbit ileal brush border (BB) receptor is a glycoprotein with an alpha-d-galactose containing trisaccharide in the toxin-binding domain (1991. J. Clin. Invest. 88:119-125). In this study we characterized the rabbit ileal BB receptor for this toxin. Purified toxin receptor peptides of 19 and 24 amino acids showed 100% homology with rabbit sucrase-isomaltase (SI). Guinea pig receptor antiserum reacted in Western blots with rabbit SI and with the purified toxin receptor. Antireceptor IgG blocked in vitro binding of toxin A to rabbit ileal villus cell BB. Furthermore, anti-SI IgG inhibited toxin A-induced secretion (by 78.1%, P < 0.01), intestinal permeability (by 80.8%, P < 0.01), and histologic injury (P < 0.01) in rabbit ileal loops in vivo. Chinese hamster ovary cells transfected with SI cDNA showed increased intracellular calcium increase in response to native toxin (holotoxin) or to a recombinant 873-amino acid peptide representing the receptor binding domain of toxin A. These data suggest that toxin A binds specifically to carbohydrate domains on rabbit ileal SI, and that such binding is relevant to signal transduction mechanisms that mediate in vitro and in vivo toxicity.
Url:
PubMed: 8698855
PubMed Central: 507473
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000459
- to stream Pmc, to step Curation: 000459
- to stream Pmc, to step Checkpoint: 000A44
- to stream Ncbi, to step Merge: 001677
- to stream Ncbi, to step Curation: 001677
- to stream Ncbi, to step Checkpoint: 001677
Links to Exploration step
PMC:507473Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rabbit sucrase-isomaltase contains a functional intestinal receptor for Clostridium difficile toxin A.</title><author><name sortKey="Pothoulakis, C" sort="Pothoulakis, C" uniqKey="Pothoulakis C" first="C" last="Pothoulakis">C. Pothoulakis</name></author><author><name sortKey="Gilbert, R J" sort="Gilbert, R J" uniqKey="Gilbert R" first="R J" last="Gilbert">R J Gilbert</name></author><author><name sortKey="Cladaras, C" sort="Cladaras, C" uniqKey="Cladaras C" first="C" last="Cladaras">C. Cladaras</name></author><author><name sortKey="Castagliuolo, I" sort="Castagliuolo, I" uniqKey="Castagliuolo I" first="I" last="Castagliuolo">I. Castagliuolo</name></author><author><name sortKey="Semenza, G" sort="Semenza, G" uniqKey="Semenza G" first="G" last="Semenza">G. Semenza</name></author><author><name sortKey="Hitti, Y" sort="Hitti, Y" uniqKey="Hitti Y" first="Y" last="Hitti">Y. Hitti</name></author><author><name sortKey="Montcrief, J S" sort="Montcrief, J S" uniqKey="Montcrief J" first="J S" last="Montcrief">J S Montcrief</name></author><author><name sortKey="Linevsky, J" sort="Linevsky, J" uniqKey="Linevsky J" first="J" last="Linevsky">J. Linevsky</name></author><author><name sortKey="Kelly, C P" sort="Kelly, C P" uniqKey="Kelly C" first="C P" last="Kelly">C P Kelly</name></author><author><name sortKey="Nikulasson, S" sort="Nikulasson, S" uniqKey="Nikulasson S" first="S" last="Nikulasson">S. Nikulasson</name></author><author><name sortKey="Desai, H P" sort="Desai, H P" uniqKey="Desai H" first="H P" last="Desai">H P Desai</name></author><author><name sortKey="Wilkins, T D" sort="Wilkins, T D" uniqKey="Wilkins T" first="T D" last="Wilkins">T D Wilkins</name></author><author><name sortKey="Lamont, J T" sort="Lamont, J T" uniqKey="Lamont J" first="J T" last="Lamont">J T Lamont</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">8698855</idno><idno type="pmc">507473</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC507473</idno><idno type="RBID">PMC:507473</idno><date when="1996">1996</date><idno type="wicri:Area/Pmc/Corpus">000459</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000459</idno><idno type="wicri:Area/Pmc/Curation">000459</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000459</idno><idno type="wicri:Area/Pmc/Checkpoint">000A44</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A44</idno><idno type="wicri:Area/Ncbi/Merge">001677</idno><idno type="wicri:Area/Ncbi/Curation">001677</idno><idno type="wicri:Area/Ncbi/Checkpoint">001677</idno><idno type="wicri:doubleKey">0021-9738:1996:Pothoulakis C:rabbit:sucrase:isomaltase</idno><idno type="wicri:Area/Main/Merge">001A14</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Rabbit sucrase-isomaltase contains a functional intestinal receptor for Clostridium difficile toxin A.</title><author><name sortKey="Pothoulakis, C" sort="Pothoulakis, C" uniqKey="Pothoulakis C" first="C" last="Pothoulakis">C. Pothoulakis</name></author><author><name sortKey="Gilbert, R J" sort="Gilbert, R J" uniqKey="Gilbert R" first="R J" last="Gilbert">R J Gilbert</name></author><author><name sortKey="Cladaras, C" sort="Cladaras, C" uniqKey="Cladaras C" first="C" last="Cladaras">C. Cladaras</name></author><author><name sortKey="Castagliuolo, I" sort="Castagliuolo, I" uniqKey="Castagliuolo I" first="I" last="Castagliuolo">I. Castagliuolo</name></author><author><name sortKey="Semenza, G" sort="Semenza, G" uniqKey="Semenza G" first="G" last="Semenza">G. Semenza</name></author><author><name sortKey="Hitti, Y" sort="Hitti, Y" uniqKey="Hitti Y" first="Y" last="Hitti">Y. Hitti</name></author><author><name sortKey="Montcrief, J S" sort="Montcrief, J S" uniqKey="Montcrief J" first="J S" last="Montcrief">J S Montcrief</name></author><author><name sortKey="Linevsky, J" sort="Linevsky, J" uniqKey="Linevsky J" first="J" last="Linevsky">J. Linevsky</name></author><author><name sortKey="Kelly, C P" sort="Kelly, C P" uniqKey="Kelly C" first="C P" last="Kelly">C P Kelly</name></author><author><name sortKey="Nikulasson, S" sort="Nikulasson, S" uniqKey="Nikulasson S" first="S" last="Nikulasson">S. Nikulasson</name></author><author><name sortKey="Desai, H P" sort="Desai, H P" uniqKey="Desai H" first="H P" last="Desai">H P Desai</name></author><author><name sortKey="Wilkins, T D" sort="Wilkins, T D" uniqKey="Wilkins T" first="T D" last="Wilkins">T D Wilkins</name></author><author><name sortKey="Lamont, J T" sort="Lamont, J T" uniqKey="Lamont J" first="J T" last="Lamont">J T Lamont</name></author></analytic><series><title level="j">Journal of Clinical Investigation</title><idno type="ISSN">0021-9738</idno><imprint><date when="1996">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The intestinal effects of Clostridium difficile toxin A are inidated by toxin binding to luminal enterocyte receptors. We reported previously that the rabbit ileal brush border (BB) receptor is a glycoprotein with an alpha-d-galactose containing trisaccharide in the toxin-binding domain (1991. J. Clin. Invest. 88:119-125). In this study we characterized the rabbit ileal BB receptor for this toxin. Purified toxin receptor peptides of 19 and 24 amino acids showed 100% homology with rabbit sucrase-isomaltase (SI). Guinea pig receptor antiserum reacted in Western blots with rabbit SI and with the purified toxin receptor. Antireceptor IgG blocked in vitro binding of toxin A to rabbit ileal villus cell BB. Furthermore, anti-SI IgG inhibited toxin A-induced secretion (by 78.1%, P < 0.01), intestinal permeability (by 80.8%, P < 0.01), and histologic injury (P < 0.01) in rabbit ileal loops in vivo. Chinese hamster ovary cells transfected with SI cDNA showed increased intracellular calcium increase in response to native toxin (holotoxin) or to a recombinant 873-amino acid peptide representing the receptor binding domain of toxin A. These data suggest that toxin A binds specifically to carbohydrate domains on rabbit ileal SI, and that such binding is relevant to signal transduction mechanisms that mediate in vitro and in vivo toxicity.</p></div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001A14 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001A14 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= Main
|étape= Merge
|type= RBID
|clé= PMC:507473
|texte= Rabbit sucrase-isomaltase contains a functional intestinal receptor for Clostridium difficile toxin A.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:8698855" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a CovidV2
| This area was generated with Dilib version V0.6.33. |  |