CRISPR-mediated activation of endogenous BST-2/tetherin expression inhibits wild-type HIV-1 production
Identifieur interne : 000D62 ( Main/Merge ); précédent : 000D61; suivant : 000D63CRISPR-mediated activation of endogenous BST-2/tetherin expression inhibits wild-type HIV-1 production
Auteurs : Yanzhao Zhang [Japon] ; Seiya Ozono [Japon] ; Weitong Yao [Japon] ; Minoru Tobiume [Japon] ; Shoji Yamaoka [Japon] ; Satoshi Kishigami [Japon] ; Hideaki Fujita [Japon] ; Kenzo Tokunaga [Japon]Source :
- Scientific Reports [ 2045-2322 ] ; 2019.
Abstract
The CRISPR technology not only can knock out target genes by using the RNA-guided Cas9 nuclease but also can activate their expression when a nuclease-deficient Cas9 (dCas9) is employed. Using the latter function, we here show the effect of the CRISPR-mediated pinpoint activation of endogenous expression of BST-2 (also known as tetherin), a virus restriction factor with a broad antiviral spectrum. Single-guide RNA (sgRNA) sequences targeting the BST-2 promoter were selected by promoter assays. Potential sgRNAs and dCas9 fused to the VP64 transactivation domain, along with an accessory transcriptional activator complex, were introduced into cells by lentiviral transduction. Increased expression of
Url:
DOI: 10.1038/s41598-019-40003-z
PubMed: 30816279
PubMed Central: 6395588
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000334
- to stream Pmc, to step Curation: 000334
- to stream Pmc, to step Checkpoint: 000269
- to stream Ncbi, to step Merge: 000848
- to stream Ncbi, to step Curation: 000848
- to stream Ncbi, to step Checkpoint: 000848
Links to Exploration step
PMC:6395588Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">CRISPR-mediated activation of endogenous BST-2/tetherin expression inhibits wild-type HIV-1 production</title>
<author><name sortKey="Zhang, Yanzhao" sort="Zhang, Yanzhao" uniqKey="Zhang Y" first="Yanzhao" last="Zhang">Yanzhao Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2220 1880</institution-id>
<institution-id institution-id-type="GRID">grid.410795.e</institution-id>
<institution>Department of Pathology,</institution>
<institution>National Institute of Infectious Diseases,</institution>
</institution-wrap>
Tokyo, 162-8640 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ozono, Seiya" sort="Ozono, Seiya" uniqKey="Ozono S" first="Seiya" last="Ozono">Seiya Ozono</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2220 1880</institution-id>
<institution-id institution-id-type="GRID">grid.410795.e</institution-id>
<institution>Department of Pathology,</institution>
<institution>National Institute of Infectious Diseases,</institution>
</institution-wrap>
Tokyo, 162-8640 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0291 3581</institution-id>
<institution-id institution-id-type="GRID">grid.267500.6</institution-id>
<institution>Faculty of Life and Environmental Sciences,</institution>
<institution>University of Yamanashi,</institution>
</institution-wrap>
Yamanashi, 400-8510 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Yamanashi</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yao, Weitong" sort="Yao, Weitong" uniqKey="Yao W" first="Weitong" last="Yao">Weitong Yao</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2220 1880</institution-id>
<institution-id institution-id-type="GRID">grid.410795.e</institution-id>
<institution>Department of Pathology,</institution>
<institution>National Institute of Infectious Diseases,</institution>
</institution-wrap>
Tokyo, 162-8640 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1014 9130</institution-id>
<institution-id institution-id-type="GRID">grid.265073.5</institution-id>
<institution>Department of Molecular Virology,</institution>
<institution>Tokyo Medical and Dental University,</institution>
</institution-wrap>
Tokyo, 113-8519 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Tobiume, Minoru" sort="Tobiume, Minoru" uniqKey="Tobiume M" first="Minoru" last="Tobiume">Minoru Tobiume</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2220 1880</institution-id>
<institution-id institution-id-type="GRID">grid.410795.e</institution-id>
<institution>Department of Pathology,</institution>
<institution>National Institute of Infectious Diseases,</institution>
</institution-wrap>
Tokyo, 162-8640 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yamaoka, Shoji" sort="Yamaoka, Shoji" uniqKey="Yamaoka S" first="Shoji" last="Yamaoka">Shoji Yamaoka</name>
<affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1014 9130</institution-id>
<institution-id institution-id-type="GRID">grid.265073.5</institution-id>
<institution>Department of Molecular Virology,</institution>
<institution>Tokyo Medical and Dental University,</institution>
</institution-wrap>
Tokyo, 113-8519 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kishigami, Satoshi" sort="Kishigami, Satoshi" uniqKey="Kishigami S" first="Satoshi" last="Kishigami">Satoshi Kishigami</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0291 3581</institution-id>
<institution-id institution-id-type="GRID">grid.267500.6</institution-id>
<institution>Faculty of Life and Environmental Sciences,</institution>
<institution>University of Yamanashi,</institution>
</institution-wrap>
Yamanashi, 400-8510 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Yamanashi</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Fujita, Hideaki" sort="Fujita, Hideaki" uniqKey="Fujita H" first="Hideaki" last="Fujita">Hideaki Fujita</name>
<affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0647 5488</institution-id>
<institution-id institution-id-type="GRID">grid.411871.a</institution-id>
<institution>Faculty of Pharmaceutical Sciences,</institution>
<institution>Nagasaki International University,</institution>
</institution-wrap>
Nagasaki, 859-3298 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Nagasaki</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Tokunaga, Kenzo" sort="Tokunaga, Kenzo" uniqKey="Tokunaga K" first="Kenzo" last="Tokunaga">Kenzo Tokunaga</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2220 1880</institution-id>
<institution-id institution-id-type="GRID">grid.410795.e</institution-id>
<institution>Department of Pathology,</institution>
<institution>National Institute of Infectious Diseases,</institution>
</institution-wrap>
Tokyo, 162-8640 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">30816279</idno>
<idno type="pmc">6395588</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395588</idno>
<idno type="RBID">PMC:6395588</idno>
<idno type="doi">10.1038/s41598-019-40003-z</idno>
<date when="2019">2019</date>
<idno type="wicri:Area/Pmc/Corpus">000334</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000334</idno>
<idno type="wicri:Area/Pmc/Curation">000334</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000334</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000269</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000269</idno>
<idno type="wicri:Area/Ncbi/Merge">000848</idno>
<idno type="wicri:Area/Ncbi/Curation">000848</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000848</idno>
<idno type="wicri:Area/Main/Merge">000D62</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">CRISPR-mediated activation of endogenous BST-2/tetherin expression inhibits wild-type HIV-1 production</title>
<author><name sortKey="Zhang, Yanzhao" sort="Zhang, Yanzhao" uniqKey="Zhang Y" first="Yanzhao" last="Zhang">Yanzhao Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2220 1880</institution-id>
<institution-id institution-id-type="GRID">grid.410795.e</institution-id>
<institution>Department of Pathology,</institution>
<institution>National Institute of Infectious Diseases,</institution>
</institution-wrap>
Tokyo, 162-8640 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ozono, Seiya" sort="Ozono, Seiya" uniqKey="Ozono S" first="Seiya" last="Ozono">Seiya Ozono</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2220 1880</institution-id>
<institution-id institution-id-type="GRID">grid.410795.e</institution-id>
<institution>Department of Pathology,</institution>
<institution>National Institute of Infectious Diseases,</institution>
</institution-wrap>
Tokyo, 162-8640 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0291 3581</institution-id>
<institution-id institution-id-type="GRID">grid.267500.6</institution-id>
<institution>Faculty of Life and Environmental Sciences,</institution>
<institution>University of Yamanashi,</institution>
</institution-wrap>
Yamanashi, 400-8510 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Yamanashi</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yao, Weitong" sort="Yao, Weitong" uniqKey="Yao W" first="Weitong" last="Yao">Weitong Yao</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2220 1880</institution-id>
<institution-id institution-id-type="GRID">grid.410795.e</institution-id>
<institution>Department of Pathology,</institution>
<institution>National Institute of Infectious Diseases,</institution>
</institution-wrap>
Tokyo, 162-8640 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1014 9130</institution-id>
<institution-id institution-id-type="GRID">grid.265073.5</institution-id>
<institution>Department of Molecular Virology,</institution>
<institution>Tokyo Medical and Dental University,</institution>
</institution-wrap>
Tokyo, 113-8519 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Tobiume, Minoru" sort="Tobiume, Minoru" uniqKey="Tobiume M" first="Minoru" last="Tobiume">Minoru Tobiume</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2220 1880</institution-id>
<institution-id institution-id-type="GRID">grid.410795.e</institution-id>
<institution>Department of Pathology,</institution>
<institution>National Institute of Infectious Diseases,</institution>
</institution-wrap>
Tokyo, 162-8640 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Yamaoka, Shoji" sort="Yamaoka, Shoji" uniqKey="Yamaoka S" first="Shoji" last="Yamaoka">Shoji Yamaoka</name>
<affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 1014 9130</institution-id>
<institution-id institution-id-type="GRID">grid.265073.5</institution-id>
<institution>Department of Molecular Virology,</institution>
<institution>Tokyo Medical and Dental University,</institution>
</institution-wrap>
Tokyo, 113-8519 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kishigami, Satoshi" sort="Kishigami, Satoshi" uniqKey="Kishigami S" first="Satoshi" last="Kishigami">Satoshi Kishigami</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0291 3581</institution-id>
<institution-id institution-id-type="GRID">grid.267500.6</institution-id>
<institution>Faculty of Life and Environmental Sciences,</institution>
<institution>University of Yamanashi,</institution>
</institution-wrap>
Yamanashi, 400-8510 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Yamanashi</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Fujita, Hideaki" sort="Fujita, Hideaki" uniqKey="Fujita H" first="Hideaki" last="Fujita">Hideaki Fujita</name>
<affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0647 5488</institution-id>
<institution-id institution-id-type="GRID">grid.411871.a</institution-id>
<institution>Faculty of Pharmaceutical Sciences,</institution>
<institution>Nagasaki International University,</institution>
</institution-wrap>
Nagasaki, 859-3298 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Nagasaki</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Tokunaga, Kenzo" sort="Tokunaga, Kenzo" uniqKey="Tokunaga K" first="Kenzo" last="Tokunaga">Kenzo Tokunaga</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2220 1880</institution-id>
<institution-id institution-id-type="GRID">grid.410795.e</institution-id>
<institution>Department of Pathology,</institution>
<institution>National Institute of Infectious Diseases,</institution>
</institution-wrap>
Tokyo, 162-8640 Japan</nlm:aff>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Tokyo</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Scientific Reports</title>
<idno type="eISSN">2045-2322</idno>
<imprint><date when="2019">2019</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="Par1">The CRISPR technology not only can knock out target genes by using the RNA-guided Cas9 nuclease but also can activate their expression when a nuclease-deficient Cas9 (dCas9) is employed. Using the latter function, we here show the effect of the CRISPR-mediated pinpoint activation of endogenous expression of BST-2 (also known as tetherin), a virus restriction factor with a broad antiviral spectrum. Single-guide RNA (sgRNA) sequences targeting the BST-2 promoter were selected by promoter assays. Potential sgRNAs and dCas9 fused to the VP64 transactivation domain, along with an accessory transcriptional activator complex, were introduced into cells by lentiviral transduction. Increased expression of <italic>BST-2</italic>
mRNA in transduced cells was confirmed by real-time RT-PCR. Cells in which BST-2 expression was highly enhanced showed the effective inhibition of HIV-1 production and replication even in the presence of the viral antagonist Vpu against BST-2. These findings confirm that the physiological stoichiometry between host restriction factors and viral antagonists may determine the outcome of the battle with viruses.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Tokunaga, K" uniqKey="Tokunaga K">K Tokunaga</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sakai, H" uniqKey="Sakai H">H Sakai</name>
</author>
<author><name sortKey="Tokunaga, K" uniqKey="Tokunaga K">K Tokunaga</name>
</author>
<author><name sortKey="Kawamura, M" uniqKey="Kawamura M">M Kawamura</name>
</author>
<author><name sortKey="Adachi, A" uniqKey="Adachi A">A Adachi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kawamura, M" uniqKey="Kawamura M">M Kawamura</name>
</author>
<author><name sortKey="Sakai, H" uniqKey="Sakai H">H Sakai</name>
</author>
<author><name sortKey="Adachi, A" uniqKey="Adachi A">A Adachi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Geraghty, Rj" uniqKey="Geraghty R">RJ Geraghty</name>
</author>
<author><name sortKey="Talbot, Kj" uniqKey="Talbot K">KJ Talbot</name>
</author>
<author><name sortKey="Callahan, M" uniqKey="Callahan M">M Callahan</name>
</author>
<author><name sortKey="Harper, W" uniqKey="Harper W">W Harper</name>
</author>
<author><name sortKey="Panganiban, At" uniqKey="Panganiban A">AT Panganiban</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sakai, H" uniqKey="Sakai H">H Sakai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gabuzda, Dh" uniqKey="Gabuzda D">DH Gabuzda</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sheehy, Am" uniqKey="Sheehy A">AM Sheehy</name>
</author>
<author><name sortKey="Gaddis, Nc" uniqKey="Gaddis N">NC Gaddis</name>
</author>
<author><name sortKey="Choi, Jd" uniqKey="Choi J">JD Choi</name>
</author>
<author><name sortKey="Malim, Mh" uniqKey="Malim M">MH Malim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zheng, Yh" uniqKey="Zheng Y">YH Zheng</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wiegand, Hl" uniqKey="Wiegand H">HL Wiegand</name>
</author>
<author><name sortKey="Doehle, Bp" uniqKey="Doehle B">BP Doehle</name>
</author>
<author><name sortKey="Bogerd, Hp" uniqKey="Bogerd H">HP Bogerd</name>
</author>
<author><name sortKey="Cullen, Br" uniqKey="Cullen B">BR Cullen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Harris, Rs" uniqKey="Harris R">RS Harris</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Neil, Sj" uniqKey="Neil S">SJ Neil</name>
</author>
<author><name sortKey="Zang, T" uniqKey="Zang T">T Zang</name>
</author>
<author><name sortKey="Bieniasz, Pd" uniqKey="Bieniasz P">PD Bieniasz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Damme, N" uniqKey="Van Damme N">N Van Damme</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Laguette, N" uniqKey="Laguette N">N Laguette</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hrecka, K" uniqKey="Hrecka K">K Hrecka</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rosa, A" uniqKey="Rosa A">A Rosa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Usami, Y" uniqKey="Usami Y">Y Usami</name>
</author>
<author><name sortKey="Wu, Y" uniqKey="Wu Y">Y Wu</name>
</author>
<author><name sortKey="Gottlinger, Hg" uniqKey="Gottlinger H">HG Gottlinger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sakuma, T" uniqKey="Sakuma T">T Sakuma</name>
</author>
<author><name sortKey="Noda, T" uniqKey="Noda T">T Noda</name>
</author>
<author><name sortKey="Urata, S" uniqKey="Urata S">S Urata</name>
</author>
<author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name>
</author>
<author><name sortKey="Yasuda, J" uniqKey="Yasuda J">J Yasuda</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Radoshitzky, Sr" uniqKey="Radoshitzky S">SR Radoshitzky</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kaletsky, Rl" uniqKey="Kaletsky R">RL Kaletsky</name>
</author>
<author><name sortKey="Francica, Jr" uniqKey="Francica J">JR Francica</name>
</author>
<author><name sortKey="Agrawal Gamse, C" uniqKey="Agrawal Gamse C">C Agrawal-Gamse</name>
</author>
<author><name sortKey="Bates, P" uniqKey="Bates P">P Bates</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lopez, La" uniqKey="Lopez L">LA Lopez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bampi, C" uniqKey="Bampi C">C Bampi</name>
</author>
<author><name sortKey="Rasga, L" uniqKey="Rasga L">L Rasga</name>
</author>
<author><name sortKey="Roux, L" uniqKey="Roux L">L Roux</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yondola, Ma" uniqKey="Yondola M">MA Yondola</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mangeat, B" uniqKey="Mangeat B">B Mangeat</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Blondeau, C" uniqKey="Blondeau C">C Blondeau</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mansouri, M" uniqKey="Mansouri M">M Mansouri</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Weidner, Jm" uniqKey="Weidner J">JM Weidner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jones, Ph" uniqKey="Jones P">PH Jones</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Taylor, Jk" uniqKey="Taylor J">JK Taylor</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yan, R" uniqKey="Yan R">R Yan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dafa Berger, A" uniqKey="Dafa Berger A">A Dafa-Berger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ohta, K" uniqKey="Ohta K">K Ohta</name>
</author>
<author><name sortKey="Goto, H" uniqKey="Goto H">H Goto</name>
</author>
<author><name sortKey="Yumine, N" uniqKey="Yumine N">N Yumine</name>
</author>
<author><name sortKey="Nishio, M" uniqKey="Nishio M">M Nishio</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lavender, Kj" uniqKey="Lavender K">KJ Lavender</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pillai, Sk" uniqKey="Pillai S">SK Pillai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Biasin, M" uniqKey="Biasin M">M Biasin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jin, X" uniqKey="Jin X">X Jin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Konermann, S" uniqKey="Konermann S">S Konermann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Arias, Jf" uniqKey="Arias J">JF Arias</name>
</author>
<author><name sortKey="Iwabu, Y" uniqKey="Iwabu Y">Y Iwabu</name>
</author>
<author><name sortKey="Tokunaga, K" uniqKey="Tokunaga K">K Tokunaga</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Matsuda, A" uniqKey="Matsuda A">A Matsuda</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mcnatt, Mw" uniqKey="Mcnatt M">MW McNatt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Goffinet, C" uniqKey="Goffinet C">C Goffinet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shindo, K" uniqKey="Shindo K">K Shindo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mariani, R" uniqKey="Mariani R">R Mariani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sandler, Ng" uniqKey="Sandler N">NG Sandler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stoddart, Ca" uniqKey="Stoddart C">CA Stoddart</name>
</author>
<author><name sortKey="Keir, Me" uniqKey="Keir M">ME Keir</name>
</author>
<author><name sortKey="Mccune, Jm" uniqKey="Mccune J">JM McCune</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fernandez, S" uniqKey="Fernandez S">S Fernandez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Haas, Dw" uniqKey="Haas D">DW Haas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fraietta, Ja" uniqKey="Fraietta J">JA Fraietta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bogerd, Hp" uniqKey="Bogerd H">HP Bogerd</name>
</author>
<author><name sortKey="Kornepati, Av" uniqKey="Kornepati A">AV Kornepati</name>
</author>
<author><name sortKey="Marshall, Jb" uniqKey="Marshall J">JB Marshall</name>
</author>
<author><name sortKey="Kennedy, Em" uniqKey="Kennedy E">EM Kennedy</name>
</author>
<author><name sortKey="Cullen, Br" uniqKey="Cullen B">BR Cullen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mahauad Fernandez, Wd" uniqKey="Mahauad Fernandez W">WD Mahauad-Fernandez</name>
</author>
<author><name sortKey="Okeoma, Cm" uniqKey="Okeoma C">CM Okeoma</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhou, H" uniqKey="Zhou H">H Zhou</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liao, H K" uniqKey="Liao H">H-K Liao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Labun, K" uniqKey="Labun K">K Labun</name>
</author>
<author><name sortKey="Montague, Tg" uniqKey="Montague T">TG Montague</name>
</author>
<author><name sortKey="Gagnon, Ja" uniqKey="Gagnon J">JA Gagnon</name>
</author>
<author><name sortKey="Thyme, Sb" uniqKey="Thyme S">SB Thyme</name>
</author>
<author><name sortKey="Valen, E" uniqKey="Valen E">E Valen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bego, Mg" uniqKey="Bego M">MG Bego</name>
</author>
<author><name sortKey="Mercier, J" uniqKey="Mercier J">J Mercier</name>
</author>
<author><name sortKey="Cohen, Ea" uniqKey="Cohen E">ÉA Cohen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dominguez, Aa" uniqKey="Dominguez A">AA Dominguez</name>
</author>
<author><name sortKey="Lim, Wa" uniqKey="Lim W">WA Lim</name>
</author>
<author><name sortKey="Qi, Ls" uniqKey="Qi L">LS Qi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gilbert, La" uniqKey="Gilbert L">LA Gilbert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Iwabu, Y" uniqKey="Iwabu Y">Y Iwabu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tada, T" uniqKey="Tada T">T Tada</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Masuyama, N" uniqKey="Masuyama N">N Masuyama</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D62 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000D62 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV2 |flux= Main |étape= Merge |type= RBID |clé= PMC:6395588 |texte= CRISPR-mediated activation of endogenous BST-2/tetherin expression inhibits wild-type HIV-1 production }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:30816279" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a CovidV2
![]() | This area was generated with Dilib version V0.6.33. | ![]() |