Interplay between carbohydrate in the stalk and the length of the connecting peptide determines the cleavability of influenza virus hemagglutinin.
Identifieur interne : 001C31 ( Main/Exploration ); précédent : 001C30; suivant : 001C32Interplay between carbohydrate in the stalk and the length of the connecting peptide determines the cleavability of influenza virus hemagglutinin.
Auteurs : Y. Kawaoka ; R G WebsterSource :
- Journal of Virology [ 0022-538X ] ; 1989.
Abstract
The ability of many viruses to replicate in host cells depends on cleavage of certain viral glycoproteins, including hemagglutinin (HA). By generating site-specific mutant HAs of two highly virulent influenza viruses, we established that the relationship between carbohydrate in the stalk and the length of the connecting peptide is a critical determinant of cleavability. HAs that lacked an oligosaccharide side chain in the stalk were cleaved regardless of the number of basic amino acids at the cleavage site, whereas those with the oligosaccharide side chain resisted cleavage unless additional basic amino acids were inserted. This finding suggests that the oligosaccharide side chain interferes with HA cleavage if the number of basic amino acids at the cleavage site is not adequate to nullify this effect. Similar interplay could influence cleavage of other viral glycoproteins, such as those of human and simian immunodeficiency viruses and paramyxoviruses.
Url:
PubMed: 2746732
PubMed Central: 250901
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000473
- to stream Pmc, to step Curation: 000473
- to stream Pmc, to step Checkpoint: 000A74
- to stream Ncbi, to step Merge: 000633
- to stream Ncbi, to step Curation: 000633
- to stream Ncbi, to step Checkpoint: 000633
- to stream Main, to step Merge: 001C51
- to stream Main, to step Curation: 001C31
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Interplay between carbohydrate in the stalk and the length of the connecting peptide determines the cleavability of influenza virus hemagglutinin.</title>
<author><name sortKey="Kawaoka, Y" sort="Kawaoka, Y" uniqKey="Kawaoka Y" first="Y" last="Kawaoka">Y. Kawaoka</name>
</author>
<author><name sortKey="Webster, R G" sort="Webster, R G" uniqKey="Webster R" first="R G" last="Webster">R G Webster</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">2746732</idno>
<idno type="pmc">250901</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC250901</idno>
<idno type="RBID">PMC:250901</idno>
<date when="1989">1989</date>
<idno type="wicri:Area/Pmc/Corpus">000473</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000473</idno>
<idno type="wicri:Area/Pmc/Curation">000473</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000473</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000A74</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A74</idno>
<idno type="wicri:Area/Ncbi/Merge">000633</idno>
<idno type="wicri:Area/Ncbi/Curation">000633</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000633</idno>
<idno type="wicri:doubleKey">0022-538X:1989:Kawaoka Y:interplay:between:carbohydrate</idno>
<idno type="wicri:Area/Main/Merge">001C51</idno>
<idno type="wicri:Area/Main/Curation">001C31</idno>
<idno type="wicri:Area/Main/Exploration">001C31</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Interplay between carbohydrate in the stalk and the length of the connecting peptide determines the cleavability of influenza virus hemagglutinin.</title>
<author><name sortKey="Kawaoka, Y" sort="Kawaoka, Y" uniqKey="Kawaoka Y" first="Y" last="Kawaoka">Y. Kawaoka</name>
</author>
<author><name sortKey="Webster, R G" sort="Webster, R G" uniqKey="Webster R" first="R G" last="Webster">R G Webster</name>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="1989">1989</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The ability of many viruses to replicate in host cells depends on cleavage of certain viral glycoproteins, including hemagglutinin (HA). By generating site-specific mutant HAs of two highly virulent influenza viruses, we established that the relationship between carbohydrate in the stalk and the length of the connecting peptide is a critical determinant of cleavability. HAs that lacked an oligosaccharide side chain in the stalk were cleaved regardless of the number of basic amino acids at the cleavage site, whereas those with the oligosaccharide side chain resisted cleavage unless additional basic amino acids were inserted. This finding suggests that the oligosaccharide side chain interferes with HA cleavage if the number of basic amino acids at the cleavage site is not adequate to nullify this effect. Similar interplay could influence cleavage of other viral glycoproteins, such as those of human and simian immunodeficiency viruses and paramyxoviruses.</p>
<sec sec-type="scanned-figures"><title>Images</title>
<fig id="F1"><graphic xlink:href="jvirol00075-0097-a" xlink:role="3297"></graphic>
</fig>
<fig id="F2"><graphic xlink:href="jvirol00075-0098-a" xlink:role="3298"></graphic>
</fig>
<fig id="F3"><graphic xlink:href="jvirol00075-0098-b" xlink:role="3298"></graphic>
</fig>
<fig id="F4"><graphic xlink:href="jvirol00075-0099-a" xlink:role="3299"></graphic>
</fig>
</sec>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Kawaoka, Y" sort="Kawaoka, Y" uniqKey="Kawaoka Y" first="Y" last="Kawaoka">Y. Kawaoka</name>
<name sortKey="Webster, R G" sort="Webster, R G" uniqKey="Webster R" first="R G" last="Webster">R G Webster</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C31 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001C31 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV2 |flux= Main |étape= Exploration |type= RBID |clé= PMC:250901 |texte= Interplay between carbohydrate in the stalk and the length of the connecting peptide determines the cleavability of influenza virus hemagglutinin. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:2746732" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a CovidV2
This area was generated with Dilib version V0.6.33. |