Tissue chambers — a useful model for in vivo studies of cytokine production in the pig
Identifieur interne : 001982 ( Main/Exploration ); précédent : 001981; suivant : 001983Tissue chambers — a useful model for in vivo studies of cytokine production in the pig
Auteurs : Eva Wattrang [Suède] ; Per Wallgren [Suède] ; Lisbeth Fuxler [Suède] ; Marie Lindersson [Suède] ; Caroline Fossum [Suède]Source :
- Veterinary Immunology and Immunopathology [ 0165-2427 ] ; 1997.
English descriptors
- Teeft :
- Agricultural sciences, Animal health, Artursson, Becton dickinson, Bengtsson, Blood cells, Cell numbers, Cell preparations, Cell recruitment, Chamber wall, Chambers, Chemiluminescence assay, Connective tissue, Cytokine, Cytokine inducers, Cytokine production, Cytokine responses, Disease virus, Eosinophilic granulocytes, Experimental animals, Experimental pigs, First injection, Flank, Flow cytometric analysis, Frequent sample collection, Functional capacity, Functional tests, Genetic variation, Granulation tissue, Granulocyte, Growth medium, High levels, Higher levels, Immune, Immune reactions, Immune response, Immune responses, Immunol, Immunology, Immunopathology, Inducer, Inflammatory cells, Injection, Inner diameter, Interferon, Interferon induction, Jensen pharmaceutics, Larger size, Lateral neck, Leukocyte, Light emission, Limited number, Local cytokine production, Local production, Lowest levels, Mil2, Moderate infiltration, Monoclonal antibodies, Mononuclear, Mononuclear cells, Months post surgery, Nacl, Neutrophil, Neutrophilic granulocytes, Peak value, Perforated chambers, Pig, Poly, Poly injection, Porcine, Porcine cytokines, Porcine leukocytes, Post injection, Present study, Primary injection, Right flank, Right neck, Sample collection, Sample collection procedure, Sample collections, Second injection, Sialistic rubber tubing, Skin wound, Slight modifications, Smaller size, Subcutaneous tissue, Such chambers, Time points, Tissue chamber, Tissue chamber fluid, Tissue chamber model, Tissue chambers, Total number, Total numbers, Tumor necrosis factor, Untreated, Untreated chambers, Uppsala, Veterinary, Veterinary immunology, Veterinary medicine, Vivo studies, Wattrang.
Abstract
Abstract: An in vivo tissue chamber model was developed to enable studies of local cytokine production and cellular events during inflammatory and immune reactions in the pig. Tissue chambers made of sialistic rubber tubing were surgically implanted in the subcutaneous tissue and samples of tissue chamber fluid (TCF) and inflammatory cells were collected by aspiration with a syringe. To evaluate the model for local cytokine production, two cytokine inducers, polyribinosinic-polyribocytidylic acid (poly I:C) and fixed Aujeszky's disease virus infected PK15 cells (ADV-PK15), were injected into the tissue chambers and samples of TCF were collected 0, 4, 8, 12, 24 and 48 h post injection. Poly I:C injections induced local production of interferon-α (IFN-α) as well as tumor necrosis factor (TNF) in the TCF but kinetic differences in the production of the cytokines were noted. Poly I:C also induced an increase in cell numbers in the TCF, mainly due to increased neutrophil numbers. Injections of ADV-PK15 induced local IFN-α production in the TCF as long as the pigs were serologically negative to ADV. Immunofluorescence and in situ hybridization techniques could be applied for characterization of TCF cells. Moreover, cells recovered from the tissue chambers were viable and could be used in functional in vitro tests. Taken together, this tissue chamber model could prove very useful in in vivo studies of inflammatory/immune responses and cytokine production in the pig.
Url:
DOI: 10.1016/S0165-2427(96)05733-9
Affiliations:
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Le document en format XML
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Division of Immunology, Swedish University of Agricultural Sciences, BMC, Box 588, S-751 23 Uppsala</wicri:regionArea><wicri:noRegion>S-751 23 Uppsala</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Veterinary Immunology and Immunopathology</title><title level="j" type="abbrev">VETIMM</title><idno type="ISSN">0165-2427</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997">1997</date><biblScope unit="volume">56</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="133">133</biblScope><biblScope unit="page" to="150">150</biblScope></imprint><idno type="ISSN">0165-2427</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0165-2427</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Agricultural sciences</term><term>Animal health</term><term>Artursson</term><term>Becton dickinson</term><term>Bengtsson</term><term>Blood cells</term><term>Cell numbers</term><term>Cell preparations</term><term>Cell recruitment</term><term>Chamber wall</term><term>Chambers</term><term>Chemiluminescence assay</term><term>Connective tissue</term><term>Cytokine</term><term>Cytokine inducers</term><term>Cytokine production</term><term>Cytokine responses</term><term>Disease virus</term><term>Eosinophilic granulocytes</term><term>Experimental animals</term><term>Experimental pigs</term><term>First injection</term><term>Flank</term><term>Flow cytometric analysis</term><term>Frequent sample collection</term><term>Functional capacity</term><term>Functional tests</term><term>Genetic variation</term><term>Granulation tissue</term><term>Granulocyte</term><term>Growth medium</term><term>High levels</term><term>Higher levels</term><term>Immune</term><term>Immune reactions</term><term>Immune response</term><term>Immune responses</term><term>Immunol</term><term>Immunology</term><term>Immunopathology</term><term>Inducer</term><term>Inflammatory cells</term><term>Injection</term><term>Inner diameter</term><term>Interferon</term><term>Interferon induction</term><term>Jensen pharmaceutics</term><term>Larger size</term><term>Lateral neck</term><term>Leukocyte</term><term>Light emission</term><term>Limited number</term><term>Local cytokine production</term><term>Local production</term><term>Lowest levels</term><term>Mil2</term><term>Moderate infiltration</term><term>Monoclonal antibodies</term><term>Mononuclear</term><term>Mononuclear cells</term><term>Months post surgery</term><term>Nacl</term><term>Neutrophil</term><term>Neutrophilic granulocytes</term><term>Peak value</term><term>Perforated chambers</term><term>Pig</term><term>Poly</term><term>Poly injection</term><term>Porcine</term><term>Porcine cytokines</term><term>Porcine leukocytes</term><term>Post injection</term><term>Present study</term><term>Primary injection</term><term>Right flank</term><term>Right neck</term><term>Sample collection</term><term>Sample collection procedure</term><term>Sample collections</term><term>Second injection</term><term>Sialistic rubber tubing</term><term>Skin wound</term><term>Slight modifications</term><term>Smaller size</term><term>Subcutaneous tissue</term><term>Such chambers</term><term>Time points</term><term>Tissue chamber</term><term>Tissue chamber fluid</term><term>Tissue chamber model</term><term>Tissue chambers</term><term>Total number</term><term>Total numbers</term><term>Tumor necrosis factor</term><term>Untreated</term><term>Untreated chambers</term><term>Uppsala</term><term>Veterinary</term><term>Veterinary immunology</term><term>Veterinary medicine</term><term>Vivo studies</term><term>Wattrang</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: An in vivo tissue chamber model was developed to enable studies of local cytokine production and cellular events during inflammatory and immune reactions in the pig. Tissue chambers made of sialistic rubber tubing were surgically implanted in the subcutaneous tissue and samples of tissue chamber fluid (TCF) and inflammatory cells were collected by aspiration with a syringe. To evaluate the model for local cytokine production, two cytokine inducers, polyribinosinic-polyribocytidylic acid (poly I:C) and fixed Aujeszky's disease virus infected PK15 cells (ADV-PK15), were injected into the tissue chambers and samples of TCF were collected 0, 4, 8, 12, 24 and 48 h post injection. Poly I:C injections induced local production of interferon-α (IFN-α) as well as tumor necrosis factor (TNF) in the TCF but kinetic differences in the production of the cytokines were noted. Poly I:C also induced an increase in cell numbers in the TCF, mainly due to increased neutrophil numbers. Injections of ADV-PK15 induced local IFN-α production in the TCF as long as the pigs were serologically negative to ADV. Immunofluorescence and in situ hybridization techniques could be applied for characterization of TCF cells. Moreover, cells recovered from the tissue chambers were viable and could be used in functional in vitro tests. Taken together, this tissue chamber model could prove very useful in in vivo studies of inflammatory/immune responses and cytokine production in the pig.</div></front></TEI><affiliations><list><country><li>Suède</li></country></list><tree><country name="Suède"><noRegion><name sortKey="Wattrang, Eva" sort="Wattrang, Eva" uniqKey="Wattrang E" first="Eva" last="Wattrang">Eva Wattrang</name></noRegion><name sortKey="Fossum, Caroline" sort="Fossum, Caroline" uniqKey="Fossum C" first="Caroline" last="Fossum">Caroline Fossum</name><name sortKey="Fuxler, Lisbeth" sort="Fuxler, Lisbeth" uniqKey="Fuxler L" first="Lisbeth" last="Fuxler">Lisbeth Fuxler</name><name sortKey="Lindersson, Marie" sort="Lindersson, Marie" uniqKey="Lindersson M" first="Marie" last="Lindersson">Marie Lindersson</name><name sortKey="Wallgren, Per" sort="Wallgren, Per" uniqKey="Wallgren P" first="Per" last="Wallgren">Per Wallgren</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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