Recent advances in proteolysis and peptide/protein separation by chromatographic strategies
Identifieur interne : 001488 ( Main/Exploration ); précédent : 001487; suivant : 001489Recent advances in proteolysis and peptide/protein separation by chromatographic strategies
Auteurs : Xiangmin Zhang [République populaire de Chine] ; Baohong Liu [République populaire de Chine] ; Lihua Zhang [République populaire de Chine] ; Hanfa Zou [République populaire de Chine] ; Jing Cao [République populaire de Chine] ; Mingxia Gao [République populaire de Chine] ; Jia Tang [République populaire de Chine] ; Yun Liu [République populaire de Chine] ; Pengyuan Yang [République populaire de Chine] ; Yukui Zhang [République populaire de Chine]Source :
- Science China Chemistry [ 1674-7291 ] ; 2010-04-01.
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Abstract
Abstract: This review gives a broad glance on the progress of recent advances on proteolysis and peptide/protein separation by chromatographic strategies in the past ten years, covering the main research in these areas especially in China. The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. These advances have significantly promoted the research of protein/peptide separation and identification in proteomics.
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DOI: 10.1007/s11426-010-0135-7
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The reviewed research focused on enzymatic micro-reactors and peptide separation in bottom-up approaches, and protein and peptide separation in top-down approaches. The new enzymatic micro-reactor is able to accelerate proteolytic reaction rate from conventionally a couple of hours to a few seconds, and the multiple dimensional chromatographic-separation with various models or arrays could sufficiently separate the proteomic mixture. These advances have significantly promoted the research of protein/peptide separation and identification in proteomics.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Xiangmin" sort="Zhang, Xiangmin" uniqKey="Zhang X" first="Xiangmin" last="Zhang">Xiangmin Zhang</name></noRegion><name sortKey="Cao, Jing" sort="Cao, Jing" uniqKey="Cao J" first="Jing" last="Cao">Jing Cao</name><name sortKey="Gao, Mingxia" sort="Gao, Mingxia" uniqKey="Gao M" first="Mingxia" last="Gao">Mingxia Gao</name><name sortKey="Liu, Baohong" sort="Liu, Baohong" uniqKey="Liu B" first="Baohong" last="Liu">Baohong Liu</name><name sortKey="Liu, Yun" sort="Liu, Yun" uniqKey="Liu Y" first="Yun" last="Liu">Yun Liu</name><name sortKey="Tang, Jia" sort="Tang, Jia" uniqKey="Tang J" first="Jia" last="Tang">Jia Tang</name><name sortKey="Yang, Pengyuan" sort="Yang, Pengyuan" uniqKey="Yang P" first="Pengyuan" last="Yang">Pengyuan Yang</name><name sortKey="Yang, Pengyuan" sort="Yang, Pengyuan" uniqKey="Yang P" first="Pengyuan" last="Yang">Pengyuan Yang</name><name sortKey="Zhang, Lihua" sort="Zhang, Lihua" uniqKey="Zhang L" first="Lihua" last="Zhang">Lihua Zhang</name><name sortKey="Zhang, Yukui" sort="Zhang, Yukui" uniqKey="Zhang Y" first="Yukui" last="Zhang">Yukui Zhang</name><name sortKey="Zhang, Yukui" sort="Zhang, Yukui" uniqKey="Zhang Y" first="Yukui" last="Zhang">Yukui Zhang</name><name sortKey="Zou, Hanfa" sort="Zou, Hanfa" uniqKey="Zou H" first="Hanfa" last="Zou">Hanfa Zou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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