Polyinosine‐polycytidylic acid enhances cellular adherence of Streptococcus pneumoniae
Identifieur interne : 001421 ( Main/Exploration ); précédent : 001420; suivant : 001422Polyinosine‐polycytidylic acid enhances cellular adherence of Streptococcus pneumoniae
Auteurs : Masaki Kawabata [Japon] ; Yuichi Kurono [Japon]Source :
- The Laryngoscope [ 0023-852X ] ; 2011-11.
Abstract
Objectives/Hypothesis:: Viral upper respiratory tract infections (URIs) are often followed by secondary bacterial infections. To better understand this phenomenon, we examined the impact of the viral agent polyinosine‐polycytidylic acid [Poly (I:C)] on the adherence of Streptococcus pneumoniae (Spn) to pharyngeal epithelial cells. Study Design:: In vitro model of cultured human pharyngeal epithelial cells. Methods:: Detroit 562 cells, a human pharyngeal carcinoma cell line, were pretreated with Poly (I:C). Poly (I:C)–induced expression of platelet‐activating factor receptor (PAF‐R) was assayed using real‐time polymerase chain reaction, flow cytometry, and immunofluorescence microscopy. Bacterial adhesion to these epithelial cells was assessed using immunofluorescence microscopy and colony formation assays. Results:: Pretreatment with Poly (I:C) increased mRNA and protein expression of PAF‐R in Detroit 562 cells and enhanced the adherence of Spn to these epithelial cells. Conclusions:: RNA viral infection can enhance PAF‐R expression in epithelial cells and increase the adherence of Spn. These findings might explain in part the mechanisms that underlie the increase in bacterial infection following URIs.
Url:
DOI: 10.1002/lary.22328
Affiliations:
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Polyinosine‐polycytidylic acid enhances cellular adherence of Streptococcus pneumoniae</title><author><name sortKey="Kawabata, Masaki" sort="Kawabata, Masaki" uniqKey="Kawabata M" first="Masaki" last="Kawabata">Masaki Kawabata</name></author><author><name sortKey="Kurono, Yuichi" sort="Kurono, Yuichi" uniqKey="Kurono Y" first="Yuichi" last="Kurono">Yuichi Kurono</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8E62AB7FCFE24BB2CA6B6C5CA8A7D53ABEC56681</idno><date when="2011" year="2011">2011</date><idno type="doi">10.1002/lary.22328</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-TJP04GJ4-G/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000518</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000518</idno><idno type="wicri:Area/Istex/Curation">000485</idno><idno type="wicri:Area/Istex/Checkpoint">000081</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000081</idno><idno type="wicri:doubleKey">0023-852X:2011:Kawabata M:polyinosine:polycytidylic:acid</idno><idno type="wicri:Area/Main/Merge">001423</idno><idno type="wicri:Area/Main/Curation">001421</idno><idno type="wicri:Area/Main/Exploration">001421</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Polyinosine‐polycytidylic acid enhances cellular adherence of Streptococcus pneumoniae</title><author><name sortKey="Kawabata, Masaki" sort="Kawabata, Masaki" uniqKey="Kawabata M" first="Masaki" last="Kawabata">Masaki Kawabata</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Otolaryngology–Head and Neck Surgery, Kagoshima University, Graduate School of Medical and Dental Science, Kagoshima</wicri:regionArea><wicri:noRegion>Kagoshima</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Japon</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Correspondence address: Department of Otolaryngology, Head and Neck Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, 8‐35‐1, Kagoshima 890‐8520</wicri:regionArea><wicri:noRegion>Kagoshima 890‐8520</wicri:noRegion></affiliation></author><author><name sortKey="Kurono, Yuichi" sort="Kurono, Yuichi" uniqKey="Kurono Y" first="Yuichi" last="Kurono">Yuichi Kurono</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Otolaryngology–Head and Neck Surgery, Kagoshima University, Graduate School of Medical and Dental Science, Kagoshima</wicri:regionArea><wicri:noRegion>Kagoshima</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Laryngoscope</title><title level="j" type="alt">LARYNGOSCOPE</title><idno type="ISSN">0023-852X</idno><idno type="eISSN">1531-4995</idno><imprint><biblScope unit="vol">121</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="2443">2443</biblScope><biblScope unit="page" to="2448">2448</biblScope><biblScope unit="page-count">6</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2011-11">2011-11</date></imprint><idno type="ISSN">0023-852X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0023-852X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Objectives/Hypothesis:: Viral upper respiratory tract infections (URIs) are often followed by secondary bacterial infections. To better understand this phenomenon, we examined the impact of the viral agent polyinosine‐polycytidylic acid [Poly (I:C)] on the adherence of Streptococcus pneumoniae (Spn) to pharyngeal epithelial cells. Study Design:: In vitro model of cultured human pharyngeal epithelial cells. Methods:: Detroit 562 cells, a human pharyngeal carcinoma cell line, were pretreated with Poly (I:C). Poly (I:C)–induced expression of platelet‐activating factor receptor (PAF‐R) was assayed using real‐time polymerase chain reaction, flow cytometry, and immunofluorescence microscopy. Bacterial adhesion to these epithelial cells was assessed using immunofluorescence microscopy and colony formation assays. Results:: Pretreatment with Poly (I:C) increased mRNA and protein expression of PAF‐R in Detroit 562 cells and enhanced the adherence of Spn to these epithelial cells. Conclusions:: RNA viral infection can enhance PAF‐R expression in epithelial cells and increase the adherence of Spn. These findings might explain in part the mechanisms that underlie the increase in bacterial infection following URIs.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Kawabata, Masaki" sort="Kawabata, Masaki" uniqKey="Kawabata M" first="Masaki" last="Kawabata">Masaki Kawabata</name></noRegion><name sortKey="Kawabata, Masaki" sort="Kawabata, Masaki" uniqKey="Kawabata M" first="Masaki" last="Kawabata">Masaki Kawabata</name><name sortKey="Kawabata, Masaki" sort="Kawabata, Masaki" uniqKey="Kawabata M" first="Masaki" last="Kawabata">Masaki Kawabata</name><name sortKey="Kurono, Yuichi" sort="Kurono, Yuichi" uniqKey="Kurono Y" first="Yuichi" last="Kurono">Yuichi Kurono</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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