Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections
Identifieur interne : 001182 ( Main/Exploration ); précédent : 001181; suivant : 001183Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections
Auteurs : Nasirah Banu ; Adeline Chia ; Zi Zong Ho ; Alfonso Tan Garcia ; Komathi Paravasivam ; Gijsbert M. Grotenbreg ; Antonio Bertoletti ; Adam J. GehringSource :
- Scientific Reports [ 2045-2322 ] ; 2014.
Abstract
Restoration of antigen-specific T cell immunity has the potential to clear persistent viral infection. T cell receptor (TCR) gene therapy can reconstitute CD8 T cell immunity in chronic patients. We cloned 10 virus-specific TCRs targeting 5 different viruses, causing chronic and acute infection. All 10 TCR genetic constructs were optimized for expression using a P2A sequence, codon optimization and the addition of a non-native disulfide bond. However, maximum TCR expression was only achieved after establishing the optimal orientation of the alpha and beta chains in the expression cassette; 9/10 TCRs favored the beta-P2A-alpha orientation over alpha-P2A-beta. Optimal TCR expression was associated with a significant increase in the frequency of IFN-gamma+ T cells. In addition, activating cells for transduction in the presence of Toll-like receptor ligands further enhanced IFN-gamma production. Thus, we have built a virus-specific TCR library that has potential for therapeutic intervention in chronic viral infection or virus-related cancers.
Url:
DOI: 10.1038/srep04166
PubMed: 24566718
PubMed Central: 3933865
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000325
- to stream Pmc, to step Curation: 000325
- to stream Pmc, to step Checkpoint: 000650
- to stream Ncbi, to step Merge: 000427
- to stream Ncbi, to step Curation: 000427
- to stream Ncbi, to step Checkpoint: 000427
- to stream Main, to step Merge: 001184
- to stream Main, to step Curation: 001182
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections</title><author><name sortKey="Banu, Nasirah" sort="Banu, Nasirah" uniqKey="Banu N" first="Nasirah" last="Banu">Nasirah Banu</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Chia, Adeline" sort="Chia, Adeline" uniqKey="Chia A" first="Adeline" last="Chia">Adeline Chia</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Ho, Zi Zong" sort="Ho, Zi Zong" uniqKey="Ho Z" first="Zi Zong" last="Ho">Zi Zong Ho</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Garcia, Alfonso Tan" sort="Garcia, Alfonso Tan" uniqKey="Garcia A" first="Alfonso Tan" last="Garcia">Alfonso Tan Garcia</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Paravasivam, Komathi" sort="Paravasivam, Komathi" uniqKey="Paravasivam K" first="Komathi" last="Paravasivam">Komathi Paravasivam</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Grotenbreg, Gijsbert M" sort="Grotenbreg, Gijsbert M" uniqKey="Grotenbreg G" first="Gijsbert M." last="Grotenbreg">Gijsbert M. Grotenbreg</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.4280.e</institution-id><institution-id institution-id-type="ISNI">0000 0001 2180 6431</institution-id><institution>Departments of Microbiology and Biological Sciences,</institution><institution>Immunology Programme, National University of Singapore,</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Bertoletti, Antonio" sort="Bertoletti, Antonio" uniqKey="Bertoletti A" first="Antonio" last="Bertoletti">Antonio Bertoletti</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation><affiliation><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.4280.e</institution-id><institution-id institution-id-type="ISNI">0000 0001 2180 6431</institution-id><institution>Program of Emerging Viral Diseases, Duke-NUS Graduate Medical School, National University of Singapore,</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Gehring, Adam J" sort="Gehring, Adam J" uniqKey="Gehring A" first="Adam J." last="Gehring">Adam J. Gehring</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation><affiliation><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="GRID">grid.262962.b</institution-id><institution-id institution-id-type="ISNI">0000 0004 1936 9342</institution-id><institution>Molecular Microbiology and Immunology, Saint Louis University School of Medicine,</institution></institution-wrap>Saint Louis, Missouri USA</nlm:aff><wicri:noCountry code="subfield">Missouri USA</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="GRID">grid.262962.b</institution-id><institution-id institution-id-type="ISNI">0000 0004 1936 9342</institution-id><institution>Saint Louis University Liver Center, Saint Louis University School of Medicine,</institution></institution-wrap>Saint Louis, Missouri USA</nlm:aff><wicri:noCountry code="subfield">Missouri USA</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24566718</idno><idno type="pmc">3933865</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933865</idno><idno type="RBID">PMC:3933865</idno><idno type="doi">10.1038/srep04166</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000325</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000325</idno><idno type="wicri:Area/Pmc/Curation">000325</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000325</idno><idno type="wicri:Area/Pmc/Checkpoint">000650</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000650</idno><idno type="wicri:Area/Ncbi/Merge">000427</idno><idno type="wicri:Area/Ncbi/Curation">000427</idno><idno type="wicri:Area/Ncbi/Checkpoint">000427</idno><idno type="wicri:Area/Main/Merge">001184</idno><idno type="wicri:Area/Main/Curation">001182</idno><idno type="wicri:Area/Main/Exploration">001182</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections</title><author><name sortKey="Banu, Nasirah" sort="Banu, Nasirah" uniqKey="Banu N" first="Nasirah" last="Banu">Nasirah Banu</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Chia, Adeline" sort="Chia, Adeline" uniqKey="Chia A" first="Adeline" last="Chia">Adeline Chia</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Ho, Zi Zong" sort="Ho, Zi Zong" uniqKey="Ho Z" first="Zi Zong" last="Ho">Zi Zong Ho</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Garcia, Alfonso Tan" sort="Garcia, Alfonso Tan" uniqKey="Garcia A" first="Alfonso Tan" last="Garcia">Alfonso Tan Garcia</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Paravasivam, Komathi" sort="Paravasivam, Komathi" uniqKey="Paravasivam K" first="Komathi" last="Paravasivam">Komathi Paravasivam</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Grotenbreg, Gijsbert M" sort="Grotenbreg, Gijsbert M" uniqKey="Grotenbreg G" first="Gijsbert M." last="Grotenbreg">Gijsbert M. Grotenbreg</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.4280.e</institution-id><institution-id institution-id-type="ISNI">0000 0001 2180 6431</institution-id><institution>Departments of Microbiology and Biological Sciences,</institution><institution>Immunology Programme, National University of Singapore,</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Bertoletti, Antonio" sort="Bertoletti, Antonio" uniqKey="Bertoletti A" first="Antonio" last="Bertoletti">Antonio Bertoletti</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation><affiliation><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.4280.e</institution-id><institution-id institution-id-type="ISNI">0000 0001 2180 6431</institution-id><institution>Program of Emerging Viral Diseases, Duke-NUS Graduate Medical School, National University of Singapore,</institution></institution-wrap>Singapore</nlm:aff></affiliation></author><author><name sortKey="Gehring, Adam J" sort="Gehring, Adam J" uniqKey="Gehring A" first="Adam J." last="Gehring">Adam J. Gehring</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.452264.3</institution-id><institution-id institution-id-type="ISNI">0000 0004 0530 269X</institution-id><institution>Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A*Star),</institution></institution-wrap>Singapore</nlm:aff></affiliation><affiliation><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="GRID">grid.262962.b</institution-id><institution-id institution-id-type="ISNI">0000 0004 1936 9342</institution-id><institution>Molecular Microbiology and Immunology, Saint Louis University School of Medicine,</institution></institution-wrap>Saint Louis, Missouri USA</nlm:aff><wicri:noCountry code="subfield">Missouri USA</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="GRID">grid.262962.b</institution-id><institution-id institution-id-type="ISNI">0000 0004 1936 9342</institution-id><institution>Saint Louis University Liver Center, Saint Louis University School of Medicine,</institution></institution-wrap>Saint Louis, Missouri USA</nlm:aff><wicri:noCountry code="subfield">Missouri USA</wicri:noCountry></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Restoration of antigen-specific T cell immunity has the potential to clear persistent viral infection. T cell receptor (TCR) gene therapy can reconstitute CD8 T cell immunity in chronic patients. We cloned 10 virus-specific TCRs targeting 5 different viruses, causing chronic and acute infection. All 10 TCR genetic constructs were optimized for expression using a P2A sequence, codon optimization and the addition of a non-native disulfide bond. However, maximum TCR expression was only achieved after establishing the optimal orientation of the alpha and beta chains in the expression cassette; 9/10 TCRs favored the beta-P2A-alpha orientation over alpha-P2A-beta. Optimal TCR expression was associated with a significant increase in the frequency of IFN-gamma+ T cells. In addition, activating cells for transduction in the presence of Toll-like receptor ligands further enhanced IFN-gamma production. Thus, we have built a virus-specific TCR library that has potential for therapeutic intervention in chronic viral infection or virus-related cancers.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Bertoletti, A" uniqKey="Bertoletti A">A Bertoletti</name></author><author><name sortKey="Ferrari, C" uniqKey="Ferrari C">C Ferrari</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pagliara, D" uniqKey="Pagliara D">D Pagliara</name></author><author><name sortKey="Savoldo, B" uniqKey="Savoldo B">B Savoldo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Gk" uniqKey="Lau G">GK Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Gkk" uniqKey="Lau G">GKK Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Loggi, E" uniqKey="Loggi E">E Loggi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cobbold, M" uniqKey="Cobbold M">M Cobbold</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rooney, Cm" uniqKey="Rooney C">CM Rooney</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Sridhar, S" uniqKey="Sridhar S">S Sridhar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oh, Hlj" uniqKey="Oh H">HLJ Oh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gehring, Aj" uniqKey="Gehring A">AJ Gehring</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sung, W K" uniqKey="Sung W">W-K Sung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Szymczak, Al" uniqKey="Szymczak A">AL Szymczak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, S" uniqKey="Yang S">S Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuball, J" uniqKey="Kuball J">J Kuball</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cohen, Cj" uniqKey="Cohen C">CJ Cohen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scholten, Kbj" uniqKey="Scholten K">KBJ Scholten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Engels, B" uniqKey="Engels B">B Engels</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Leisegang, M" uniqKey="Leisegang M">M Leisegang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Caron, G" uniqKey="Caron G">G Caron</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, Q" uniqKey="Zhu Q">Q Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haga Friedman, A" uniqKey="Haga Friedman A">A Haga-Friedman</name></author><author><name sortKey="Horovitz Fried, M" uniqKey="Horovitz Fried M">M Horovitz-Fried</name></author><author><name sortKey="Cohen, Cj" uniqKey="Cohen C">CJ Cohen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Okamoto, S" uniqKey="Okamoto S">S Okamoto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aggen, Dh" uniqKey="Aggen D">DH Aggen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sommermeyer, D" uniqKey="Sommermeyer D">D Sommermeyer</name></author><author><name sortKey="Uckert, W" uniqKey="Uckert W">W Uckert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bialer, G" uniqKey="Bialer G">G Bialer</name></author><author><name sortKey="Horovitz Fried, M" uniqKey="Horovitz Fried M">M Horovitz-Fried</name></author><author><name sortKey="Ya Acobi, S" uniqKey="Ya Acobi S">S Ya'acobi</name></author><author><name sortKey="Morgan, Ra" uniqKey="Morgan R">RA Morgan</name></author><author><name sortKey="Cohen, Cj" uniqKey="Cohen C">CJ Cohen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Voss, R H" uniqKey="Voss R">R-H Voss</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sebestyen, Z" uniqKey="Sebestyen Z">Z Sebestyén</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cohen, Cj" uniqKey="Cohen C">CJ Cohen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, S" uniqKey="Yang S">S Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hinrichs, Cs" uniqKey="Hinrichs C">CS Hinrichs</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Koh, S" uniqKey="Koh S">S Koh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kearse, Kp" uniqKey="Kearse K">KP Kearse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bonifacino, Js" uniqKey="Bonifacino J">JS Bonifacino</name></author><author><name sortKey="Suzuki, Ck" uniqKey="Suzuki C">CK Suzuki</name></author><author><name sortKey="Klausner, Rd" uniqKey="Klausner R">RD Klausner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shin, J" uniqKey="Shin J">J Shin</name></author><author><name sortKey="Lee, S" uniqKey="Lee S">S Lee</name></author><author><name sortKey="Strominger, Jl" uniqKey="Strominger J">JL Strominger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Labrecque, N" uniqKey="Labrecque N">N Labrecque</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Leen, Am" uniqKey="Leen A">AM Leen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chang, Cxl" uniqKey="Chang C">CXL Chang</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Banu, Nasirah" sort="Banu, Nasirah" uniqKey="Banu N" first="Nasirah" last="Banu">Nasirah Banu</name><name sortKey="Bertoletti, Antonio" sort="Bertoletti, Antonio" uniqKey="Bertoletti A" first="Antonio" last="Bertoletti">Antonio Bertoletti</name><name sortKey="Chia, Adeline" sort="Chia, Adeline" uniqKey="Chia A" first="Adeline" last="Chia">Adeline Chia</name><name sortKey="Garcia, Alfonso Tan" sort="Garcia, Alfonso Tan" uniqKey="Garcia A" first="Alfonso Tan" last="Garcia">Alfonso Tan Garcia</name><name sortKey="Gehring, Adam J" sort="Gehring, Adam J" uniqKey="Gehring A" first="Adam J." last="Gehring">Adam J. Gehring</name><name sortKey="Grotenbreg, Gijsbert M" sort="Grotenbreg, Gijsbert M" uniqKey="Grotenbreg G" first="Gijsbert M." last="Grotenbreg">Gijsbert M. Grotenbreg</name><name sortKey="Ho, Zi Zong" sort="Ho, Zi Zong" uniqKey="Ho Z" first="Zi Zong" last="Ho">Zi Zong Ho</name><name sortKey="Paravasivam, Komathi" sort="Paravasivam, Komathi" uniqKey="Paravasivam K" first="Komathi" last="Paravasivam">Komathi Paravasivam</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001182 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001182 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3933865
|texte= Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24566718" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a CovidV2
| This area was generated with Dilib version V0.6.33. |  |