Establishment and Application of a Universal Coronavirus Screening Method Using MALDI-TOF Mass Spectrometry
Identifieur interne : 000F06 ( Main/Exploration ); précédent : 000F05; suivant : 000F07Establishment and Application of a Universal Coronavirus Screening Method Using MALDI-TOF Mass Spectrometry
Auteurs : Leshan Xiu ; Chi Zhang ; Zhiqiang Wu ; Junping PengSource :
- Frontiers in Microbiology [ 1664-302X ] ; 2017.
Abstract
There are four human coronaviruses (HCoVs), distributed worldwide, that are associated with a range of respiratory symptoms. The discovery of severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV shows that HCoVs pose a significant threat to human health. Our work aims to develop a sensitive method (mCoV-MS) which can not only identify known HCoVs accurately, but also have the ability to provide clues for the emerging HCoVs. The method was performed using a MassARRAY matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system. We developed a 17-plex analysis to detect six HCoVs in Panel A and another 17-plex analysis to detect
Url:
DOI: 10.3389/fmicb.2017.01510
PubMed: 28848521
PubMed Central: 5552709
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Establishment and Application of a Universal Coronavirus Screening Method Using MALDI-TOF Mass Spectrometry</title><author><name sortKey="Xiu, Leshan" sort="Xiu, Leshan" uniqKey="Xiu L" first="Leshan" last="Xiu">Leshan Xiu</name></author><author><name sortKey="Zhang, Chi" sort="Zhang, Chi" uniqKey="Zhang C" first="Chi" last="Zhang">Chi Zhang</name></author><author><name sortKey="Wu, Zhiqiang" sort="Wu, Zhiqiang" uniqKey="Wu Z" first="Zhiqiang" last="Wu">Zhiqiang Wu</name></author><author><name sortKey="Peng, Junping" sort="Peng, Junping" uniqKey="Peng J" first="Junping" last="Peng">Junping Peng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28848521</idno><idno type="pmc">5552709</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5552709</idno><idno type="RBID">PMC:5552709</idno><idno type="doi">10.3389/fmicb.2017.01510</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000615</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000615</idno><idno type="wicri:Area/Pmc/Curation">000615</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000615</idno><idno type="wicri:Area/Pmc/Checkpoint">000410</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000410</idno><idno type="wicri:Area/Ncbi/Merge">000732</idno><idno type="wicri:Area/Ncbi/Curation">000732</idno><idno type="wicri:Area/Ncbi/Checkpoint">000732</idno><idno type="wicri:Area/Main/Merge">000F08</idno><idno type="wicri:Area/Main/Curation">000F06</idno><idno type="wicri:Area/Main/Exploration">000F06</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Establishment and Application of a Universal Coronavirus Screening Method Using MALDI-TOF Mass Spectrometry</title><author><name sortKey="Xiu, Leshan" sort="Xiu, Leshan" uniqKey="Xiu L" first="Leshan" last="Xiu">Leshan Xiu</name></author><author><name sortKey="Zhang, Chi" sort="Zhang, Chi" uniqKey="Zhang C" first="Chi" last="Zhang">Chi Zhang</name></author><author><name sortKey="Wu, Zhiqiang" sort="Wu, Zhiqiang" uniqKey="Wu Z" first="Zhiqiang" last="Wu">Zhiqiang Wu</name></author><author><name sortKey="Peng, Junping" sort="Peng, Junping" uniqKey="Peng J" first="Junping" last="Peng">Junping Peng</name></author></analytic><series><title level="j">Frontiers in Microbiology</title><idno type="eISSN">1664-302X</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>There are four human coronaviruses (HCoVs), distributed worldwide, that are associated with a range of respiratory symptoms. The discovery of severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV shows that HCoVs pose a significant threat to human health. Our work aims to develop a sensitive method (mCoV-MS) which can not only identify known HCoVs accurately, but also have the ability to provide clues for the emerging HCoVs. The method was performed using a MassARRAY matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system. We developed a 17-plex analysis to detect six HCoVs in Panel A and another 17-plex analysis to detect <italic>Alphacoronavirus</italic> and <italic>Betacoronavirus</italic> in Panel B. All tested primers and probes for the mCoV-MS method were effective, with no cross-reactivity observed with other common respiratory viruses. To confirm the usefulness of the mCoV-MS method we screened 384 pharyngeal and/or anal swab samples collected from bats/rodents, and 131 nasal and throat swabs from human patients. The results showed good concordance with the results of metagenomic analysis or PCR-sequencing. The validation test showed mCoV-MS method can detect potentially pathogenic CoVs in <italic>Alphacoronavirus</italic> and <italic>Betacoronavirus</italic> and provide convincingly phylogenetic evidences about unknown CoVs. The mCoV-MS method is a sensitive assay that is relatively simple to carry out. We propose that this method be used to complement next generation sequencing technology for large-scale screening studies.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Alagaili, A N" uniqKey="Alagaili A">A. N. Alagaili</name></author><author><name sortKey="Briese, T" uniqKey="Briese T">T. Briese</name></author><author><name sortKey="Mishra, N" uniqKey="Mishra N">N. Mishra</name></author><author><name sortKey="Kapoor, V" uniqKey="Kapoor V">V. Kapoor</name></author><author><name sortKey="Sameroff, S C" uniqKey="Sameroff S">S. C. 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Chen</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Peng, Junping" sort="Peng, Junping" uniqKey="Peng J" first="Junping" last="Peng">Junping Peng</name><name sortKey="Wu, Zhiqiang" sort="Wu, Zhiqiang" uniqKey="Wu Z" first="Zhiqiang" last="Wu">Zhiqiang Wu</name><name sortKey="Xiu, Leshan" sort="Xiu, Leshan" uniqKey="Xiu L" first="Leshan" last="Xiu">Leshan Xiu</name><name sortKey="Zhang, Chi" sort="Zhang, Chi" uniqKey="Zhang C" first="Chi" last="Zhang">Chi Zhang</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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