Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis
Identifieur interne : 001148 ( Main/Curation ); précédent : 001147; suivant : 001149Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis
Auteurs : Hosam Zaghloul ; Mahmoud El-ShahatSource :
- World Journal of Hepatology [ 1948-5182 ] ; 2014.
Abstract
Hepatitis C virus (HCV) infection represents a significant health problem and represents a heavy load on some countries like Egypt in which about 20% of the total population are infected. Initial infection is usually asymptomatic and result in chronic hepatitis that give rise to complications including cirrhosis and hepatocellular carcinoma. The management of HCV infection should not only be focus on therapy, but also to screen carrier individuals in order to prevent transmission. In the present, molecular detection and quantification of HCV genome by real time polymerase chain reaction (PCR) represent the gold standard in HCV diagnosis and plays a crucial role in the management of therapeutic regimens. However, real time PCR is a complicated approach and of limited distribution. On the other hand, isothermal DNA amplification techniques have been developed and offer molecular diagnosis of infectious dieses at point-of-care. In this review we discuss recombinase polymerase amplification technique and illustrate its diagnostic value over both PCR and other isothermal amplification techniques.
Url:
DOI: 10.4254/wjh.v6.i12.916
PubMed: 25544878
PubMed Central: 4269910
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000A68
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :000A68
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000620
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000483
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :000483
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000483
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :001150
Links to Exploration step
PMC:4269910Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis</title>
<author><name sortKey="Zaghloul, Hosam" sort="Zaghloul, Hosam" uniqKey="Zaghloul H" first="Hosam" last="Zaghloul">Hosam Zaghloul</name>
</author>
<author><name sortKey="El Shahat, Mahmoud" sort="El Shahat, Mahmoud" uniqKey="El Shahat M" first="Mahmoud" last="El-Shahat">Mahmoud El-Shahat</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25544878</idno>
<idno type="pmc">4269910</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269910</idno>
<idno type="RBID">PMC:4269910</idno>
<idno type="doi">10.4254/wjh.v6.i12.916</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000A68</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000A68</idno>
<idno type="wicri:Area/Pmc/Curation">000A68</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000A68</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000620</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000620</idno>
<idno type="wicri:Area/Ncbi/Merge">000483</idno>
<idno type="wicri:Area/Ncbi/Curation">000483</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000483</idno>
<idno type="wicri:doubleKey">1948-5182:2014:Zaghloul H:recombinase:polymerase:amplification</idno>
<idno type="wicri:Area/Main/Merge">001150</idno>
<idno type="wicri:Area/Main/Curation">001148</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis</title>
<author><name sortKey="Zaghloul, Hosam" sort="Zaghloul, Hosam" uniqKey="Zaghloul H" first="Hosam" last="Zaghloul">Hosam Zaghloul</name>
</author>
<author><name sortKey="El Shahat, Mahmoud" sort="El Shahat, Mahmoud" uniqKey="El Shahat M" first="Mahmoud" last="El-Shahat">Mahmoud El-Shahat</name>
</author>
</analytic>
<series><title level="j">World Journal of Hepatology</title>
<idno type="ISSN">1948-5182</idno>
<idno type="eISSN">1948-5182</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Hepatitis C virus (HCV) infection represents a significant health problem and represents a heavy load on some countries like Egypt in which about 20% of the total population are infected. Initial infection is usually asymptomatic and result in chronic hepatitis that give rise to complications including cirrhosis and hepatocellular carcinoma. The management of HCV infection should not only be focus on therapy, but also to screen carrier individuals in order to prevent transmission. In the present, molecular detection and quantification of HCV genome by real time polymerase chain reaction (PCR) represent the gold standard in HCV diagnosis and plays a crucial role in the management of therapeutic regimens. However, real time PCR is a complicated approach and of limited distribution. On the other hand, isothermal DNA amplification techniques have been developed and offer molecular diagnosis of infectious dieses at point-of-care. In this review we discuss recombinase polymerase amplification technique and illustrate its diagnostic value over both PCR and other isothermal amplification techniques.</p>
</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001148 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 001148 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= CovidV2 |flux= Main |étape= Curation |type= RBID |clé= PMC:4269910 |texte= Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:25544878" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a CovidV2
This area was generated with Dilib version V0.6.33. |