[Enhanced luminescence intensity in immunofluorescent preparations following trypsin treatment].
Identifieur interne : 000748 ( PubMed/Corpus ); précédent : 000747; suivant : 000749[Enhanced luminescence intensity in immunofluorescent preparations following trypsin treatment].
Auteurs : B. Mitov ; M. Aleksandrov ; G K Georgiev ; R. BostandzhievaSource :
- Veterinarno-meditsinski nauki [ 0324-1068 ] ; 1986.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antigens, Viral.
- chemical , pharmacology : Trypsin.
- Animals, Cattle, Fluorescent Antibody Technique, Luminescent Measurements, Temperature, Time Factors, Virus Cultivation.
Abstract
Comparative investigations were carried out on the immunofluorescent preparations of cell cultures infected with bovine viruses--rota-, corona-, respiratory-syncytial, parainfluenza-3, adeno-1, and herpes-1--to test various fixatives and the effect of trypsin in raising the sensitivity of the immunofluorescence method. The effect of trypsin was manifested in fixation with formalin, ethanol, methanol, and acetone treated immunofluorescent preparations of cell cultures infected with rota- and adeno-viruses as well as in fixation with acetone of cultures infected with respiratory syncytial virus, parainfluenza-3 virus, and corona virus. Formalin, ethanol, and partly methanol were shown to be unsuitable for the purpose of fixation of cell culture preparations infected with viruses that contained a lipoprotein envelope. It was found that the treatment of immunofluorescent preparations with trypsin following fixation and prior to their treatment with conjugated antisera enhanced considerably the number of fluorescent cells and the intensity of fluorescence itself provided 0.1 per cent trypsin was used for 5 to 10 min at 37 degrees C--for cell culture preparations, and 0.1 per cent trypsin was used for 20 to 30 min at 37 degrees C--for paraffin sections of acetone-fixed tissues.
PubMed: 3544471
Links to Exploration step
pubmed:3544471Le document en format XML
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<author><name sortKey="Mitov, B" sort="Mitov, B" uniqKey="Mitov B" first="B" last="Mitov">B. Mitov</name>
</author>
<author><name sortKey="Aleksandrov, M" sort="Aleksandrov, M" uniqKey="Aleksandrov M" first="M" last="Aleksandrov">M. Aleksandrov</name>
</author>
<author><name sortKey="Georgiev, G K" sort="Georgiev, G K" uniqKey="Georgiev G" first="G K" last="Georgiev">G K Georgiev</name>
</author>
<author><name sortKey="Bostandzhieva, R" sort="Bostandzhieva, R" uniqKey="Bostandzhieva R" first="R" last="Bostandzhieva">R. Bostandzhieva</name>
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<date when="1986">1986</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Enhanced luminescence intensity in immunofluorescent preparations following trypsin treatment].</title>
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<author><name sortKey="Aleksandrov, M" sort="Aleksandrov, M" uniqKey="Aleksandrov M" first="M" last="Aleksandrov">M. Aleksandrov</name>
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<author><name sortKey="Georgiev, G K" sort="Georgiev, G K" uniqKey="Georgiev G" first="G K" last="Georgiev">G K Georgiev</name>
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<author><name sortKey="Bostandzhieva, R" sort="Bostandzhieva, R" uniqKey="Bostandzhieva R" first="R" last="Bostandzhieva">R. Bostandzhieva</name>
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<series><title level="j">Veterinarno-meditsinski nauki</title>
<idno type="ISSN">0324-1068</idno>
<imprint><date when="1986" type="published">1986</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antigens, Viral (analysis)</term>
<term>Cattle</term>
<term>Fluorescent Antibody Technique</term>
<term>Luminescent Measurements</term>
<term>Temperature</term>
<term>Time Factors</term>
<term>Trypsin (pharmacology)</term>
<term>Virus Cultivation</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term>
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<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Trypsin</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cattle</term>
<term>Fluorescent Antibody Technique</term>
<term>Luminescent Measurements</term>
<term>Temperature</term>
<term>Time Factors</term>
<term>Virus Cultivation</term>
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<front><div type="abstract" xml:lang="en">Comparative investigations were carried out on the immunofluorescent preparations of cell cultures infected with bovine viruses--rota-, corona-, respiratory-syncytial, parainfluenza-3, adeno-1, and herpes-1--to test various fixatives and the effect of trypsin in raising the sensitivity of the immunofluorescence method. The effect of trypsin was manifested in fixation with formalin, ethanol, methanol, and acetone treated immunofluorescent preparations of cell cultures infected with rota- and adeno-viruses as well as in fixation with acetone of cultures infected with respiratory syncytial virus, parainfluenza-3 virus, and corona virus. Formalin, ethanol, and partly methanol were shown to be unsuitable for the purpose of fixation of cell culture preparations infected with viruses that contained a lipoprotein envelope. It was found that the treatment of immunofluorescent preparations with trypsin following fixation and prior to their treatment with conjugated antisera enhanced considerably the number of fluorescent cells and the intensity of fluorescence itself provided 0.1 per cent trypsin was used for 5 to 10 min at 37 degrees C--for cell culture preparations, and 0.1 per cent trypsin was used for 20 to 30 min at 37 degrees C--for paraffin sections of acetone-fixed tissues.</div>
</front>
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<DateCompleted><Year>1987</Year>
<Month>03</Month>
<Day>20</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
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<Article PubModel="Print"><Journal><ISSN IssnType="Print">0324-1068</ISSN>
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<Issue>9</Issue>
<PubDate><Year>1986</Year>
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<Title>Veterinarno-meditsinski nauki</Title>
<ISOAbbreviation>Vet Med Nauki</ISOAbbreviation>
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<ArticleTitle>[Enhanced luminescence intensity in immunofluorescent preparations following trypsin treatment].</ArticleTitle>
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<Abstract><AbstractText>Comparative investigations were carried out on the immunofluorescent preparations of cell cultures infected with bovine viruses--rota-, corona-, respiratory-syncytial, parainfluenza-3, adeno-1, and herpes-1--to test various fixatives and the effect of trypsin in raising the sensitivity of the immunofluorescence method. The effect of trypsin was manifested in fixation with formalin, ethanol, methanol, and acetone treated immunofluorescent preparations of cell cultures infected with rota- and adeno-viruses as well as in fixation with acetone of cultures infected with respiratory syncytial virus, parainfluenza-3 virus, and corona virus. Formalin, ethanol, and partly methanol were shown to be unsuitable for the purpose of fixation of cell culture preparations infected with viruses that contained a lipoprotein envelope. It was found that the treatment of immunofluorescent preparations with trypsin following fixation and prior to their treatment with conjugated antisera enhanced considerably the number of fluorescent cells and the intensity of fluorescence itself provided 0.1 per cent trypsin was used for 5 to 10 min at 37 degrees C--for cell culture preparations, and 0.1 per cent trypsin was used for 20 to 30 min at 37 degrees C--for paraffin sections of acetone-fixed tissues.</AbstractText>
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<VernacularTitle>Povishavane intenziteta na svetene pri imunofluorestsentni preparati sled tretirane s tripsin.</VernacularTitle>
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<MedlineJournalInfo><Country>Bulgaria</Country>
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<MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName>
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<MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName>
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