Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction.
Identifieur interne : 000646 ( PubMed/Corpus ); précédent : 000645; suivant : 000647Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction.
Auteurs : Mohamed S. Al-Ayed ; Ahmed M. Asaad ; Mohamed A. Qureshi ; Mohammed S. AmeenSource :
- Saudi medical journal [ 0379-5284 ] ; 2014.
English descriptors
- KwdEn :
- Child, Preschool, Coinfection (epidemiology), Coinfection (virology), Cross-Sectional Studies, DNA, Viral (analysis), Female, Humans, Infant, Infant, Newborn, Male, Molecular Epidemiology, Multiplex Polymerase Chain Reaction, RNA, Viral (analysis), Respiratory Tract Infections (epidemiology), Respiratory Tract Infections (virology), Reverse Transcriptase Polymerase Chain Reaction, Saudi Arabia (epidemiology), Virus Diseases (epidemiology), Virus Diseases (virology), Viruses (genetics), Viruses (isolation & purification).
- MESH :
- chemical , analysis : DNA, Viral, RNA, Viral.
- geographic , epidemiology : Saudi Arabia.
- epidemiology : Coinfection, Respiratory Tract Infections, Virus Diseases.
- genetics : Viruses.
- isolation & purification : Viruses.
- virology : Coinfection, Respiratory Tract Infections, Virus Diseases.
- Child, Preschool, Cross-Sectional Studies, Female, Humans, Infant, Infant, Newborn, Male, Molecular Epidemiology, Multiplex Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
To investigate 15 respiratory viruses in children with acute respiratory tract infections (ARTIs) using multiplex reverse-transcriptase polymerase chain reaction (RT-PCR), and to analyze the clinical and epidemiological features of these viruses.
PubMed: 25399211
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction.</title><author><name sortKey="Al Ayed, Mohamed S" sort="Al Ayed, Mohamed S" uniqKey="Al Ayed M" first="Mohamed S" last="Al-Ayed">Mohamed S. Al-Ayed</name><affiliation><nlm:affiliation>Department of Pediatrics, College of Medicine, Najran University, Najran, Kingdom of Saudi Arabia. E-mail. drmzayed2000@yahoo.com.</nlm:affiliation></affiliation></author><author><name sortKey="Asaad, Ahmed M" sort="Asaad, Ahmed M" uniqKey="Asaad A" first="Ahmed M" last="Asaad">Ahmed M. Asaad</name></author><author><name sortKey="Qureshi, Mohamed A" sort="Qureshi, Mohamed A" uniqKey="Qureshi M" first="Mohamed A" last="Qureshi">Mohamed A. Qureshi</name></author><author><name sortKey="Ameen, Mohammed S" sort="Ameen, Mohammed S" uniqKey="Ameen M" first="Mohammed S" last="Ameen">Mohammed S. Ameen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25399211</idno><idno type="pmid">25399211</idno><idno type="wicri:Area/PubMed/Corpus">000646</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000646</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction.</title><author><name sortKey="Al Ayed, Mohamed S" sort="Al Ayed, Mohamed S" uniqKey="Al Ayed M" first="Mohamed S" last="Al-Ayed">Mohamed S. Al-Ayed</name><affiliation><nlm:affiliation>Department of Pediatrics, College of Medicine, Najran University, Najran, Kingdom of Saudi Arabia. E-mail. drmzayed2000@yahoo.com.</nlm:affiliation></affiliation></author><author><name sortKey="Asaad, Ahmed M" sort="Asaad, Ahmed M" uniqKey="Asaad A" first="Ahmed M" last="Asaad">Ahmed M. Asaad</name></author><author><name sortKey="Qureshi, Mohamed A" sort="Qureshi, Mohamed A" uniqKey="Qureshi M" first="Mohamed A" last="Qureshi">Mohamed A. Qureshi</name></author><author><name sortKey="Ameen, Mohammed S" sort="Ameen, Mohammed S" uniqKey="Ameen M" first="Mohammed S" last="Ameen">Mohammed S. Ameen</name></author></analytic><series><title level="j">Saudi medical journal</title><idno type="ISSN">0379-5284</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Child, Preschool</term><term>Coinfection (epidemiology)</term><term>Coinfection (virology)</term><term>Cross-Sectional Studies</term><term>DNA, Viral (analysis)</term><term>Female</term><term>Humans</term><term>Infant</term><term>Infant, Newborn</term><term>Male</term><term>Molecular Epidemiology</term><term>Multiplex Polymerase Chain Reaction</term><term>RNA, Viral (analysis)</term><term>Respiratory Tract Infections (epidemiology)</term><term>Respiratory Tract Infections (virology)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Saudi Arabia (epidemiology)</term><term>Virus Diseases (epidemiology)</term><term>Virus Diseases (virology)</term><term>Viruses (genetics)</term><term>Viruses (isolation & purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Viral</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Saudi Arabia</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coinfection</term><term>Respiratory Tract Infections</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coinfection</term><term>Respiratory Tract Infections</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Child, Preschool</term><term>Cross-Sectional Studies</term><term>Female</term><term>Humans</term><term>Infant</term><term>Infant, Newborn</term><term>Male</term><term>Molecular Epidemiology</term><term>Multiplex Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To investigate 15 respiratory viruses in children with acute respiratory tract infections (ARTIs) using multiplex reverse-transcriptase polymerase chain reaction (RT-PCR), and to analyze the clinical and epidemiological features of these viruses.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25399211</PMID><DateCompleted><Year>2015</Year><Month>10</Month><Day>06</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0379-5284</ISSN><JournalIssue CitedMedium="Internet"><Volume>35</Volume><Issue>11</Issue><PubDate><Year>2014</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Saudi medical journal</Title><ISOAbbreviation>Saudi Med J</ISOAbbreviation></Journal><ArticleTitle>Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction.</ArticleTitle><Pagination><MedlinePgn>1348-53</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVES" NlmCategory="OBJECTIVE">To investigate 15 respiratory viruses in children with acute respiratory tract infections (ARTIs) using multiplex reverse-transcriptase polymerase chain reaction (RT-PCR), and to analyze the clinical and epidemiological features of these viruses.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">In a cross-sectional study, 135 children, ≤5 years of age who presented with ARTIs in Najran Maternity and Children Hospital, Najran, Saudi Arabia between October 2012 and July 2013 were included. The clinical and sociodemographic data, and the laboratory results were recorded using a standardized questionnaire. Two nasopharyngeal swabs were collected from each child: one for bacteriological examination, and the second for viral detection using multiplex RT-PCR.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">A single viral pathogen was detected in 76 patients, viral coinfections in 9, and mixed viral and bacterial pathogens in 15. Respiratory syncytial virus was isolated in 33 patients, human rhinovirus (hRV) in 22, adenovirus (AdV) in 19, human metapneumovirus in 13, influenza virus in 10, parainfluenza virus in 7, human corona virus (hCoV) in 4, and human bocavirus in one.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Respiratory syncytial virus, hRV, and AdV were the most frequent viruses, accounting for more than two-thirds of the cases. Other viruses, such as MPV, hCoV NL63, and hCoV OC43, may play a role in pediatric ARTIs. Of significance is the potential use of multiplex RT-PCR to provide epidemiological and virological data for early detection of the emergence of novel respiratory viruses in the era of the Middle East respiratory syndrome coronavirus. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Al-Ayed</LastName><ForeName>Mohamed S</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Department of Pediatrics, College of Medicine, Najran University, Najran, Kingdom of Saudi Arabia. E-mail. drmzayed2000@yahoo.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Asaad</LastName><ForeName>Ahmed M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Qureshi</LastName><ForeName>Mohamed A</ForeName><Initials>MA</Initials></Author><Author ValidYN="Y"><LastName>Ameen</LastName><ForeName>Mohammed S</ForeName><Initials>MS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Saudi Arabia</Country><MedlineTA>Saudi Med J</MedlineTA><NlmUniqueID>7909441</NlmUniqueID><ISSNLinking>0379-5284</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002675" MajorTopicYN="N">Child, Preschool</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060085" MajorTopicYN="N">Coinfection</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003430" MajorTopicYN="N">Cross-Sectional Studies</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007223" MajorTopicYN="N">Infant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007231" MajorTopicYN="N">Infant, Newborn</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017720" MajorTopicYN="N">Molecular Epidemiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060885" MajorTopicYN="N">Multiplex Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012141" MajorTopicYN="N">Respiratory Tract Infections</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="Y">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012529" MajorTopicYN="N" Type="Geographic">Saudi Arabia</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014777" MajorTopicYN="N">Virus Diseases</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="Y">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014780" MajorTopicYN="N">Viruses</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>11</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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