CovidV1, PubMed, Checkpoint, bibRecord, 000700

Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections.

Identifieur interne : 000700 ( PubMed/Checkpoint ); précédent : 000699; suivant : 000701

Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections.

Auteurs : Aleksandar Radoni [Allemagne] ; Stefanie Thulke ; Hi-Gung Bae ; Marcel A. Müller ; Wolfgang Siegert ; Andreas Nitsche

Source :

Virology journal [ 1743-422X ] ; 2005.

RBID : pubmed:15705200

Descripteurs français

English descriptors

KwdEn :
- Cell Line, Cytomegalovirus (genetics), Cytomegalovirus (isolation & purification), Genes, Viral (genetics), Herpesvirus 6, Human (genetics), Herpesvirus 6, Human (isolation & purification), Humans, Peptidylprolyl Isomerase (genetics), Peptidylprolyl Isomerase (metabolism), Polymerase Chain Reaction (methods), Polymerase Chain Reaction (standards), Poxviridae (genetics), Poxviridae (isolation & purification), Reference Standards, SARS Virus (genetics), SARS Virus (isolation & purification), TATA-Box Binding Protein (metabolism), Virus Replication, Yellow fever virus (genetics), Yellow fever virus (isolation & purification).
MESH :
- chemical , genetics : Peptidylprolyl Isomerase.
- genetics : Cytomegalovirus, Genes, Viral, Herpesvirus 6, Human, Poxviridae, SARS Virus, Yellow fever virus.
- isolation & purification : Cytomegalovirus, Herpesvirus 6, Human, Poxviridae, SARS Virus, Yellow fever virus.
- chemical , metabolism : Peptidylprolyl Isomerase, TATA-Box Binding Protein.
- methods : Polymerase Chain Reaction.
- standards : Polymerase Chain Reaction.
- Cell Line, Humans, Reference Standards, Virus Replication.

Abstract

Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, beta-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.

DOI: 10.1186/1743-422X-2-7
PubMed: 15705200

Affiliations:

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Le document en format XML

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<term>Genes, Viral (genetics)</term>
<term>Herpesvirus 6, Human (genetics)</term>
<term>Herpesvirus 6, Human (isolation & purification)</term>
<term>Humans</term>
<term>Peptidylprolyl Isomerase (genetics)</term>
<term>Peptidylprolyl Isomerase (metabolism)</term>
<term>Polymerase Chain Reaction (methods)</term>
<term>Polymerase Chain Reaction (standards)</term>
<term>Poxviridae (genetics)</term>
<term>Poxviridae (isolation & purification)</term>
<term>Reference Standards</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
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<term>Virus Replication</term>
<term>Yellow fever virus (genetics)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Cytomegalovirus (génétique)</term>
<term>Cytomegalovirus (isolement et purification)</term>
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<term>Herpèsvirus humain de type 6 (génétique)</term>
<term>Herpèsvirus humain de type 6 (isolement et purification)</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Normes de référence</term>
<term>Peptidylpropyl isomerase (génétique)</term>
<term>Peptidylpropyl isomerase (métabolisme)</term>
<term>Poxviridae (génétique)</term>
<term>Poxviridae (isolement et purification)</term>
<term>Protéine de liaison à la boite TATA (métabolisme)</term>
<term>Réaction de polymérisation en chaîne ()</term>
<term>Réaction de polymérisation en chaîne (normes)</term>
<term>Réplication virale</term>
<term>Virus de la fièvre jaune (génétique)</term>
<term>Virus de la fièvre jaune (isolement et purification)</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (isolement et purification)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptidylprolyl Isomerase</term>
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<term>Genes, Viral</term>
<term>Herpesvirus 6, Human</term>
<term>Poxviridae</term>
<term>SARS Virus</term>
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<term>Gènes viraux</term>
<term>Herpèsvirus humain de type 6</term>
<term>Peptidylpropyl isomerase</term>
<term>Poxviridae</term>
<term>Virus de la fièvre jaune</term>
<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Cytomegalovirus</term>
<term>Herpesvirus 6, Human</term>
<term>Poxviridae</term>
<term>SARS Virus</term>
<term>Yellow fever virus</term>
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<term>Protéine de liaison à la boite TATA</term>
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<term>Reference Standards</term>
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<front><div type="abstract" xml:lang="en">Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, beta-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.</div>
</front>
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<Abstract><AbstractText>Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, beta-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.</AbstractText>
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Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections.

Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections.

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